Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF75 and GFF76 overlap by 4 nucleotides GFF76 and GFF77 overlap by 7 nucleotides GFF77 and GFF78 are separated by 1 nucleotides
GFF75 - ABC transporter, substrate-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 92,148 to 93,164
GFF75
GFF76 - Alkanesulfonates transport system permease protein, at 93,161 to 94,228
GFF76
GFF77 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 94,222 to 95,025
GFF77
GFF78 - Cytochrome c family protein, at 95,027 to 96,190
GFF78
Position (kb)
93
94
95 Strain fitness (log2 ratio)
-2
-1
0
1 at 92.622 kb on + strand, within GFF75 at 92.622 kb on + strand, within GFF75 at 92.623 kb on - strand, within GFF75 at 92.623 kb on - strand, within GFF75 at 92.925 kb on + strand, within GFF75 at 92.926 kb on - strand, within GFF75 at 92.926 kb on - strand, within GFF75 at 92.926 kb on - strand, within GFF75 at 92.926 kb on - strand, within GFF75 at 92.926 kb on - strand, within GFF75 at 92.970 kb on + strand, within GFF75 at 92.970 kb on + strand, within GFF75 at 92.971 kb on - strand, within GFF75 at 93.024 kb on + strand, within GFF75 at 93.024 kb on + strand, within GFF75 at 93.024 kb on + strand, within GFF75 at 93.025 kb on - strand, within GFF75 at 93.025 kb on - strand, within GFF75 at 93.909 kb on - strand, within GFF76 at 93.909 kb on - strand, within GFF76 at 93.909 kb on - strand, within GFF76 at 93.911 kb on + strand, within GFF76 at 93.911 kb on + strand, within GFF76 at 93.911 kb on + strand, within GFF76 at 93.911 kb on + strand, within GFF76 at 93.912 kb on - strand, within GFF76 at 93.912 kb on - strand, within GFF76 at 93.912 kb on - strand, within GFF76 at 94.080 kb on - strand, within GFF76 at 94.080 kb on - strand, within GFF76 at 94.109 kb on + strand, within GFF76 at 94.121 kb on + strand, within GFF76 at 94.121 kb on + strand, within GFF76 at 94.121 kb on + strand, within GFF76 at 94.121 kb on + strand, within GFF76 at 94.121 kb on + strand, within GFF76 at 94.122 kb on - strand at 94.122 kb on - strand at 94.122 kb on - strand at 94.122 kb on - strand at 94.147 kb on - strand at 94.534 kb on + strand, within GFF77 at 94.534 kb on + strand, within GFF77 at 94.535 kb on - strand, within GFF77 at 94.535 kb on - strand, within GFF77
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 92,622 + GFF75 0.47 -1.0 92,622 + GFF75 0.47 +0.1 92,623 - GFF75 0.47 +0.5 92,623 - GFF75 0.47 +0.4 92,925 + GFF75 0.76 +0.6 92,926 - GFF75 0.76 +0.1 92,926 - GFF75 0.76 -0.5 92,926 - GFF75 0.76 -0.8 92,926 - GFF75 0.76 -0.7 92,926 - GFF75 0.76 -0.2 92,970 + GFF75 0.81 -0.9 92,970 + GFF75 0.81 -0.6 92,971 - GFF75 0.81 -0.1 93,024 + GFF75 0.86 -0.2 93,024 + GFF75 0.86 -0.6 93,024 + GFF75 0.86 +0.2 93,025 - GFF75 0.86 +0.2 93,025 - GFF75 0.86 +1.1 93,909 - GFF76 0.70 +0.7 93,909 - GFF76 0.70 -0.5 93,909 - GFF76 0.70 -1.7 93,911 + GFF76 0.70 -0.9 93,911 + GFF76 0.70 +0.9 93,911 + GFF76 0.70 +0.5 93,911 + GFF76 0.70 -2.1 93,912 - GFF76 0.70 -0.2 93,912 - GFF76 0.70 +0.5 93,912 - GFF76 0.70 -2.7 94,080 - GFF76 0.86 -0.7 94,080 - GFF76 0.86 -0.1 94,109 + GFF76 0.89 -0.8 94,121 + GFF76 0.90 -0.4 94,121 + GFF76 0.90 -0.1 94,121 + GFF76 0.90 -1.6 94,121 + GFF76 0.90 +0.2 94,121 + GFF76 0.90 +0.3 94,122 - -0.3 94,122 - +0.5 94,122 - -0.3 94,122 - +0.7 94,147 - -1.1 94,534 + GFF77 0.39 -1.1 94,534 + GFF77 0.39 +0.4 94,535 - GFF77 0.39 +1.3 94,535 - GFF77 0.39 +0.9
Or see this region's nucleotide sequence