Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7241 and GFF7242 are separated by 16 nucleotides GFF7242 and GFF7243 are separated by 66 nucleotides GFF7243 and GFF7244 are separated by 226 nucleotides GFF7244 and GFF7245 are separated by 196 nucleotides
GFF7241 - no description, at 22,793 to 23,269
GFF7241
GFF7242 - hypothetical protein, at 23,286 to 23,660
GFF7242
GFF7243 - HtrA protease/chaperone protein, at 23,727 to 25,010
GFF7243
GFF7244 - no description, at 25,237 to 25,764
GFF7244
GFF7245 - universal stress protein, UspA family, at 25,961 to 26,383
GFF7245
Position (kb) 23
24
25
26 Strain fitness (log2 ratio) -2
-1
0
1 at 22.895 kb on + strand, within GFF7241 at 22.895 kb on + strand, within GFF7241 at 22.895 kb on + strand, within GFF7241 at 22.896 kb on - strand, within GFF7241 at 23.094 kb on - strand, within GFF7241 at 23.401 kb on + strand, within GFF7242 at 23.771 kb on - strand at 24.346 kb on + strand, within GFF7243 at 24.346 kb on + strand, within GFF7243 at 24.433 kb on + strand, within GFF7243 at 24.433 kb on + strand, within GFF7243 at 24.433 kb on + strand, within GFF7243 at 24.433 kb on + strand, within GFF7243 at 24.434 kb on - strand, within GFF7243 at 24.434 kb on - strand, within GFF7243 at 24.451 kb on + strand, within GFF7243 at 24.451 kb on + strand, within GFF7243 at 24.452 kb on - strand, within GFF7243 at 24.877 kb on - strand, within GFF7243 at 25.734 kb on - strand at 25.740 kb on - strand at 25.994 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 22,895 + GFF7241 0.21 -0.5 22,895 + GFF7241 0.21 -0.0 22,895 + GFF7241 0.21 -1.5 22,896 - GFF7241 0.22 +0.5 23,094 - GFF7241 0.63 +0.4 23,401 + GFF7242 0.31 -1.3 23,771 - +0.3 24,346 + GFF7243 0.48 -1.9 24,346 + GFF7243 0.48 +1.1 24,433 + GFF7243 0.55 +1.1 24,433 + GFF7243 0.55 -0.4 24,433 + GFF7243 0.55 -1.7 24,433 + GFF7243 0.55 -0.1 24,434 - GFF7243 0.55 -0.4 24,434 - GFF7243 0.55 +0.0 24,451 + GFF7243 0.56 +0.1 24,451 + GFF7243 0.56 -0.3 24,452 - GFF7243 0.56 +0.9 24,877 - GFF7243 0.90 +0.0 25,734 - -0.2 25,740 - +0.8 25,994 + -2.4
Or see this region's nucleotide sequence