Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF7103 and GFF7104 overlap by 4 nucleotides GFF7104 and GFF7105 overlap by 4 nucleotides GFF7105 and GFF7106 are separated by 81 nucleotides
GFF7103 - 2-hydroxyhepta-2,4-diene-1,7-dioate isomerase (EC 5.3.3.-), at 21,347 to 22,096
GFF7103
GFF7104 - 5-carboxymethyl-2-oxo-hex-3- ene-1,7-dioate decarboxylase (EC 4.1.1.68), at 22,093 to 22,743
GFF7104
GFF7105 - Homoprotocatechuate degradative operon repressor, at 22,740 to 23,171
GFF7105
GFF7106 - BUG/TctC family periplasmic protein, at 23,253 to 24,233
GFF7106
Position (kb)
22
23
24 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 21.795 kb on + strand, within GFF7103 at 21.796 kb on - strand, within GFF7103 at 21.796 kb on - strand, within GFF7103 at 21.796 kb on - strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.804 kb on + strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.805 kb on - strand, within GFF7103 at 21.924 kb on + strand, within GFF7103 at 21.924 kb on + strand, within GFF7103 at 21.925 kb on - strand, within GFF7103 at 21.925 kb on - strand, within GFF7103 at 21.925 kb on - strand, within GFF7103 at 21.925 kb on - strand, within GFF7103 at 21.925 kb on - strand, within GFF7103 at 21.925 kb on - strand, within GFF7103 at 22.203 kb on + strand, within GFF7104 at 22.334 kb on - strand, within GFF7104 at 22.439 kb on + strand, within GFF7104 at 22.440 kb on - strand, within GFF7104 at 22.481 kb on + strand, within GFF7104 at 22.481 kb on + strand, within GFF7104 at 22.481 kb on + strand, within GFF7104 at 22.481 kb on + strand, within GFF7104 at 22.481 kb on + strand, within GFF7104 at 22.481 kb on + strand, within GFF7104 at 22.482 kb on - strand, within GFF7104 at 22.482 kb on - strand, within GFF7104 at 22.482 kb on - strand, within GFF7104 at 22.482 kb on - strand, within GFF7104 at 22.482 kb on - strand, within GFF7104 at 22.482 kb on - strand, within GFF7104 at 22.482 kb on - strand, within GFF7104 at 22.607 kb on + strand, within GFF7104 at 22.607 kb on + strand, within GFF7104 at 22.607 kb on + strand, within GFF7104 at 22.607 kb on + strand, within GFF7104 at 22.607 kb on + strand, within GFF7104 at 22.607 kb on + strand, within GFF7104 at 22.608 kb on - strand, within GFF7104 at 22.608 kb on - strand, within GFF7104 at 22.608 kb on - strand, within GFF7104 at 22.608 kb on - strand, within GFF7104 at 22.637 kb on + strand, within GFF7104 at 22.637 kb on + strand, within GFF7104 at 22.637 kb on + strand, within GFF7104 at 22.637 kb on + strand, within GFF7104 at 22.638 kb on - strand, within GFF7104 at 22.638 kb on - strand, within GFF7104 at 22.703 kb on + strand at 22.703 kb on + strand at 22.703 kb on + strand at 22.703 kb on + strand at 22.703 kb on + strand at 22.704 kb on - strand at 22.704 kb on - strand at 22.704 kb on - strand at 22.704 kb on - strand at 22.704 kb on - strand at 22.704 kb on - strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.705 kb on + strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.706 kb on - strand at 22.707 kb on - strand at 22.766 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.780 kb on + strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.781 kb on - strand at 22.828 kb on + strand, within GFF7105 at 22.828 kb on + strand, within GFF7105 at 23.171 kb on + strand at 23.171 kb on + strand at 23.174 kb on + strand at 23.228 kb on + strand at 23.229 kb on - strand at 23.283 kb on - strand at 23.283 kb on - strand at 23.283 kb on - strand at 23.317 kb on + strand at 23.317 kb on + strand at 23.317 kb on + strand at 23.317 kb on + strand at 23.317 kb on + strand at 23.317 kb on + strand at 23.318 kb on - strand at 23.318 kb on - strand at 23.318 kb on - strand at 23.318 kb on - strand at 23.318 kb on - strand at 23.318 kb on - strand at 23.318 kb on - strand at 23.318 kb on - strand at 23.400 kb on + strand, within GFF7106 at 23.400 kb on + strand, within GFF7106 at 23.400 kb on + strand, within GFF7106 at 23.400 kb on + strand, within GFF7106 at 23.400 kb on + strand, within GFF7106 at 23.401 kb on - strand, within GFF7106 at 23.401 kb on - strand, within GFF7106 at 23.401 kb on - strand, within GFF7106 at 23.422 kb on + strand, within GFF7106 at 23.423 kb on - strand, within GFF7106 at 23.770 kb on + strand, within GFF7106 at 23.770 kb on + strand, within GFF7106 at 23.770 kb on + strand, within GFF7106 at 23.770 kb on + strand, within GFF7106 at 23.771 kb on - strand, within GFF7106 at 23.771 kb on - strand, within GFF7106 at 23.771 kb on - strand, within GFF7106 at 23.771 kb on - strand, within GFF7106 at 23.771 kb on - strand, within GFF7106 at 23.909 kb on - strand, within GFF7106 at 23.923 kb on + strand, within GFF7106
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 21,795 + GFF7103 0.60 +1.0 21,796 - GFF7103 0.60 +0.8 21,796 - GFF7103 0.60 -1.4 21,796 - GFF7103 0.60 -0.3 21,804 + GFF7103 0.61 -0.1 21,804 + GFF7103 0.61 +0.2 21,804 + GFF7103 0.61 -0.5 21,804 + GFF7103 0.61 -0.3 21,804 + GFF7103 0.61 +0.1 21,804 + GFF7103 0.61 +0.0 21,804 + GFF7103 0.61 -0.4 21,804 + GFF7103 0.61 -0.9 21,804 + GFF7103 0.61 -1.6 21,804 + GFF7103 0.61 +0.4 21,804 + GFF7103 0.61 -0.3 21,804 + GFF7103 0.61 -0.8 21,804 + GFF7103 0.61 +0.9 21,804 + GFF7103 0.61 +0.3 21,805 - GFF7103 0.61 -0.4 21,805 - GFF7103 0.61 -1.1 21,805 - GFF7103 0.61 -0.9 21,805 - GFF7103 0.61 -1.0 21,805 - GFF7103 0.61 +0.4 21,805 - GFF7103 0.61 +0.2 21,805 - GFF7103 0.61 +0.4 21,805 - GFF7103 0.61 -1.2 21,805 - GFF7103 0.61 -0.2 21,805 - GFF7103 0.61 +0.3 21,805 - GFF7103 0.61 -1.2 21,805 - GFF7103 0.61 +1.4 21,924 + GFF7103 0.77 +0.3 21,924 + GFF7103 0.77 +0.4 21,925 - GFF7103 0.77 +1.2 21,925 - GFF7103 0.77 +0.6 21,925 - GFF7103 0.77 -1.7 21,925 - GFF7103 0.77 -0.4 21,925 - GFF7103 0.77 +0.3 21,925 - GFF7103 0.77 +0.9 22,203 + GFF7104 0.17 -0.8 22,334 - GFF7104 0.37 -0.6 22,439 + GFF7104 0.53 +0.6 22,440 - GFF7104 0.53 -0.7 22,481 + GFF7104 0.60 +0.3 22,481 + GFF7104 0.60 +0.3 22,481 + GFF7104 0.60 -0.3 22,481 + GFF7104 0.60 -1.0 22,481 + GFF7104 0.60 -1.4 22,481 + GFF7104 0.60 -0.1 22,482 - GFF7104 0.60 -0.6 22,482 - GFF7104 0.60 +0.4 22,482 - GFF7104 0.60 +0.4 22,482 - GFF7104 0.60 +0.4 22,482 - GFF7104 0.60 -0.6 22,482 - GFF7104 0.60 -0.2 22,482 - GFF7104 0.60 +1.3 22,607 + GFF7104 0.79 +0.0 22,607 + GFF7104 0.79 -2.6 22,607 + GFF7104 0.79 -0.2 22,607 + GFF7104 0.79 +0.2 22,607 + GFF7104 0.79 -0.5 22,607 + GFF7104 0.79 -0.3 22,608 - GFF7104 0.79 -0.6 22,608 - GFF7104 0.79 -0.8 22,608 - GFF7104 0.79 +1.6 22,608 - GFF7104 0.79 -0.3 22,637 + GFF7104 0.84 -1.1 22,637 + GFF7104 0.84 +1.1 22,637 + GFF7104 0.84 -0.1 22,637 + GFF7104 0.84 -0.6 22,638 - GFF7104 0.84 +0.1 22,638 - GFF7104 0.84 +0.8 22,703 + +0.6 22,703 + +1.1 22,703 + -0.1 22,703 + -0.1 22,703 + -0.6 22,704 - +0.3 22,704 - -0.7 22,704 - -1.0 22,704 - +0.2 22,704 - -1.0 22,704 - +0.4 22,705 + -0.6 22,705 + +0.6 22,705 + -0.7 22,705 + -0.7 22,705 + -0.2 22,705 + +0.3 22,705 + +0.8 22,705 + -0.4 22,705 + -0.1 22,705 + -1.7 22,705 + -1.1 22,705 + -0.8 22,705 + -0.3 22,705 + -1.9 22,705 + +0.9 22,705 + -1.3 22,705 + +0.0 22,705 + -1.2 22,705 + -1.0 22,705 + -0.6 22,705 + -0.2 22,705 + -0.2 22,705 + -0.3 22,705 + -0.2 22,705 + -1.9 22,705 + -0.3 22,705 + +0.3 22,706 - -0.4 22,706 - -1.1 22,706 - +0.3 22,706 - -1.6 22,706 - +0.4 22,706 - +0.0 22,706 - -0.2 22,706 - -0.1 22,706 - -0.3 22,706 - -0.6 22,706 - +2.0 22,706 - +0.1 22,706 - -0.7 22,706 - +0.6 22,706 - -0.5 22,706 - -0.9 22,706 - -0.6 22,706 - +0.0 22,706 - -0.8 22,706 - +0.2 22,707 - +0.7 22,766 + +0.1 22,780 + +0.8 22,780 + -0.2 22,780 + +0.0 22,780 + -0.7 22,780 + +0.0 22,780 + -1.1 22,780 + -0.4 22,780 + +0.0 22,780 + -2.9 22,780 + +0.3 22,780 + +2.0 22,780 + -0.2 22,781 - -0.5 22,781 - -0.4 22,781 - -1.1 22,781 - +1.0 22,781 - -0.2 22,781 - -1.0 22,781 - -0.1 22,781 - -0.1 22,781 - -0.3 22,781 - -1.0 22,781 - -1.5 22,781 - -1.0 22,828 + GFF7105 0.20 +0.0 22,828 + GFF7105 0.20 +1.1 23,171 + -0.5 23,171 + -0.2 23,174 + -0.1 23,228 + +0.2 23,229 - +0.0 23,283 - -0.6 23,283 - -0.3 23,283 - -0.0 23,317 + +0.1 23,317 + -0.0 23,317 + -0.1 23,317 + +0.2 23,317 + +0.8 23,317 + -0.4 23,318 - -1.4 23,318 - +0.4 23,318 - -1.1 23,318 - +1.4 23,318 - -1.0 23,318 - -0.1 23,318 - -0.2 23,318 - -0.4 23,400 + GFF7106 0.15 +0.0 23,400 + GFF7106 0.15 -0.4 23,400 + GFF7106 0.15 -0.5 23,400 + GFF7106 0.15 +0.8 23,400 + GFF7106 0.15 -1.2 23,401 - GFF7106 0.15 +0.0 23,401 - GFF7106 0.15 +0.1 23,401 - GFF7106 0.15 +0.2 23,422 + GFF7106 0.17 +0.4 23,423 - GFF7106 0.17 -0.2 23,770 + GFF7106 0.53 -0.6 23,770 + GFF7106 0.53 +0.6 23,770 + GFF7106 0.53 -1.9 23,770 + GFF7106 0.53 +0.5 23,771 - GFF7106 0.53 -0.6 23,771 - GFF7106 0.53 -0.1 23,771 - GFF7106 0.53 +1.7 23,771 - GFF7106 0.53 -0.7 23,771 - GFF7106 0.53 -0.1 23,909 - GFF7106 0.67 -0.6 23,923 + GFF7106 0.68 -0.6
Or see this region's nucleotide sequence