Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6964 and GFF6965 are separated by 62 nucleotides GFF6965 and GFF6966 overlap by 1 nucleotides GFF6966 and GFF6967 are separated by 103 nucleotides
GFF6964 - Cytochrome c oxidase (cbb3-type) subunit CcoN (EC 1.9.3.1), at 36,983 to 38,425
GFF6964
GFF6965 - Type cbb3 cytochrome oxidase biogenesis protein CcoS, involved in heme b insertion, at 38,488 to 38,625
GFF6965
GFF6966 - Type cbb3 cytochrome oxidase biogenesis protein CcoI; Copper-translocating P-type ATPase (EC 3.6.3.4), at 38,625 to 40,955
GFF6966
GFF6967 - Universal stress protein family, at 41,059 to 41,502
GFF6967
Position (kb) 38
39
40
41 Strain fitness (log2 ratio) -2
-1
0
1
2 at 37.636 kb on - strand, within GFF6964 at 37.665 kb on + strand, within GFF6964 at 37.665 kb on + strand, within GFF6964 at 37.665 kb on + strand, within GFF6964 at 37.666 kb on - strand, within GFF6964 at 37.666 kb on - strand, within GFF6964 at 37.743 kb on + strand, within GFF6964 at 37.743 kb on + strand, within GFF6964 at 37.744 kb on - strand, within GFF6964 at 37.744 kb on - strand, within GFF6964 at 37.744 kb on - strand, within GFF6964 at 37.744 kb on - strand, within GFF6964 at 37.744 kb on - strand, within GFF6964 at 37.746 kb on + strand, within GFF6964 at 37.746 kb on + strand, within GFF6964 at 37.747 kb on - strand, within GFF6964 at 37.747 kb on - strand, within GFF6964 at 37.749 kb on + strand, within GFF6964 at 37.749 kb on + strand, within GFF6964 at 37.750 kb on - strand, within GFF6964 at 37.750 kb on - strand, within GFF6964 at 37.750 kb on - strand, within GFF6964 at 37.839 kb on + strand, within GFF6964 at 37.926 kb on + strand, within GFF6964 at 37.926 kb on + strand, within GFF6964 at 37.927 kb on - strand, within GFF6964 at 37.945 kb on - strand, within GFF6964 at 37.945 kb on - strand, within GFF6964 at 38.050 kb on - strand, within GFF6964 at 38.237 kb on - strand, within GFF6964 at 38.762 kb on - strand at 40.051 kb on - strand, within GFF6966 at 40.051 kb on - strand, within GFF6966 at 40.501 kb on + strand, within GFF6966 at 40.501 kb on + strand, within GFF6966 at 40.501 kb on + strand, within GFF6966 at 40.501 kb on + strand, within GFF6966 at 40.501 kb on + strand, within GFF6966 at 40.502 kb on - strand, within GFF6966 at 41.195 kb on - strand, within GFF6967 at 41.466 kb on - strand at 41.522 kb on + strand at 41.522 kb on + strand at 41.522 kb on + strand at 41.523 kb on - strand at 41.635 kb on - strand at 41.635 kb on - strand at 41.766 kb on + strand at 41.771 kb on + strand at 41.851 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 37,636 - GFF6964 0.45 -0.1 37,665 + GFF6964 0.47 -0.3 37,665 + GFF6964 0.47 +0.8 37,665 + GFF6964 0.47 -0.9 37,666 - GFF6964 0.47 -0.9 37,666 - GFF6964 0.47 +0.1 37,743 + GFF6964 0.53 +1.5 37,743 + GFF6964 0.53 -1.2 37,744 - GFF6964 0.53 +0.5 37,744 - GFF6964 0.53 +1.5 37,744 - GFF6964 0.53 -0.9 37,744 - GFF6964 0.53 -0.2 37,744 - GFF6964 0.53 -0.4 37,746 + GFF6964 0.53 -0.1 37,746 + GFF6964 0.53 -0.5 37,747 - GFF6964 0.53 +0.7 37,747 - GFF6964 0.53 +0.7 37,749 + GFF6964 0.53 -0.4 37,749 + GFF6964 0.53 +1.9 37,750 - GFF6964 0.53 -0.2 37,750 - GFF6964 0.53 -1.3 37,750 - GFF6964 0.53 -1.5 37,839 + GFF6964 0.59 -0.3 37,926 + GFF6964 0.65 -0.5 37,926 + GFF6964 0.65 +2.3 37,927 - GFF6964 0.65 -0.9 37,945 - GFF6964 0.67 +0.0 37,945 - GFF6964 0.67 +1.1 38,050 - GFF6964 0.74 -0.5 38,237 - GFF6964 0.87 -1.0 38,762 - -2.7 40,051 - GFF6966 0.61 -0.4 40,051 - GFF6966 0.61 +1.9 40,501 + GFF6966 0.80 -0.7 40,501 + GFF6966 0.80 -0.3 40,501 + GFF6966 0.80 -0.8 40,501 + GFF6966 0.80 +0.9 40,501 + GFF6966 0.80 -1.3 40,502 - GFF6966 0.81 -0.8 41,195 - GFF6967 0.31 +0.1 41,466 - -1.0 41,522 + +0.3 41,522 + -0.7 41,522 + -0.2 41,523 - -0.1 41,635 - -0.2 41,635 - +0.1 41,766 + -0.8 41,771 + -0.1 41,851 + -0.6
Or see this region's nucleotide sequence