Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF687 and GFF688 overlap by 4 nucleotides GFF688 and GFF689 overlap by 4 nucleotides GFF689 and GFF690 are separated by 56 nucleotides GFF690 and GFF691 are separated by 82 nucleotides
GFF687 - Dihydroxy-acid dehydratase (EC 4.2.1.9), at 741,288 to 742,988
GFF687
GFF688 - Uncharacterized conserved protein, at 742,985 to 743,437
GFF688
GFF689 - protein of unknown function DUF521, at 743,434 to 744,729
GFF689
GFF690 - Transcriptional regulator, IclR family, at 744,786 to 745,628
GFF690
GFF691 - hypothetical protein, at 745,711 to 747,162
GFF691
Position (kb)
743
744
745 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 742.528 kb on + strand, within GFF687 at 742.528 kb on + strand, within GFF687 at 742.528 kb on + strand, within GFF687 at 742.528 kb on + strand, within GFF687 at 742.529 kb on - strand, within GFF687 at 742.529 kb on - strand, within GFF687 at 742.529 kb on - strand, within GFF687 at 742.529 kb on - strand, within GFF687 at 742.529 kb on - strand, within GFF687 at 742.547 kb on - strand, within GFF687 at 742.711 kb on + strand, within GFF687 at 742.711 kb on + strand, within GFF687 at 742.711 kb on + strand, within GFF687 at 742.711 kb on + strand, within GFF687 at 742.711 kb on + strand, within GFF687 at 742.711 kb on + strand, within GFF687 at 742.712 kb on - strand, within GFF687 at 742.712 kb on - strand, within GFF687 at 742.948 kb on + strand at 742.948 kb on + strand at 742.948 kb on + strand at 742.948 kb on + strand at 742.948 kb on + strand at 742.949 kb on - strand at 742.949 kb on - strand at 743.441 kb on + strand at 743.442 kb on - strand at 743.442 kb on - strand at 743.531 kb on + strand at 743.531 kb on + strand at 743.531 kb on + strand at 743.771 kb on + strand, within GFF689 at 743.772 kb on - strand, within GFF689 at 743.772 kb on - strand, within GFF689 at 743.772 kb on - strand, within GFF689 at 743.772 kb on - strand, within GFF689 at 743.772 kb on - strand, within GFF689 at 743.772 kb on - strand, within GFF689 at 743.772 kb on - strand, within GFF689 at 743.795 kb on + strand, within GFF689 at 743.795 kb on + strand, within GFF689 at 743.795 kb on + strand, within GFF689 at 743.795 kb on + strand, within GFF689 at 743.795 kb on + strand, within GFF689 at 743.796 kb on - strand, within GFF689 at 743.796 kb on - strand, within GFF689 at 743.837 kb on + strand, within GFF689 at 743.838 kb on - strand, within GFF689 at 743.865 kb on - strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.945 kb on + strand, within GFF689 at 743.946 kb on - strand, within GFF689 at 743.946 kb on - strand, within GFF689 at 743.946 kb on - strand, within GFF689 at 743.946 kb on - strand, within GFF689 at 743.946 kb on - strand, within GFF689 at 743.946 kb on - strand, within GFF689 at 744.078 kb on - strand, within GFF689 at 744.174 kb on - strand, within GFF689 at 744.257 kb on + strand, within GFF689 at 744.257 kb on + strand, within GFF689 at 744.257 kb on + strand, within GFF689 at 744.257 kb on + strand, within GFF689 at 744.257 kb on + strand, within GFF689 at 744.257 kb on + strand, within GFF689 at 744.257 kb on + strand, within GFF689 at 744.258 kb on - strand, within GFF689 at 744.258 kb on - strand, within GFF689 at 744.258 kb on - strand, within GFF689 at 744.258 kb on - strand, within GFF689 at 744.258 kb on - strand, within GFF689 at 744.258 kb on - strand, within GFF689 at 744.258 kb on - strand, within GFF689 at 744.323 kb on + strand, within GFF689 at 744.323 kb on + strand, within GFF689 at 744.324 kb on - strand, within GFF689 at 744.386 kb on + strand, within GFF689 at 744.386 kb on + strand, within GFF689 at 744.386 kb on + strand, within GFF689 at 744.386 kb on + strand, within GFF689 at 744.644 kb on + strand at 744.645 kb on - strand at 744.698 kb on + strand at 744.698 kb on + strand at 744.698 kb on + strand at 744.698 kb on + strand at 744.699 kb on - strand at 744.699 kb on - strand at 744.699 kb on - strand at 744.699 kb on - strand at 744.699 kb on - strand at 744.699 kb on - strand at 744.699 kb on - strand at 744.787 kb on + strand at 744.788 kb on - strand at 744.788 kb on - strand at 744.807 kb on + strand at 744.807 kb on + strand at 744.808 kb on - strand at 745.003 kb on + strand, within GFF690 at 745.132 kb on + strand, within GFF690 at 745.132 kb on + strand, within GFF690 at 745.133 kb on - strand, within GFF690 at 745.133 kb on - strand, within GFF690 at 745.133 kb on - strand, within GFF690 at 745.133 kb on - strand, within GFF690 at 745.237 kb on + strand, within GFF690 at 745.262 kb on - strand, within GFF690 at 745.262 kb on - strand, within GFF690 at 745.667 kb on + strand at 745.667 kb on + strand at 745.667 kb on + strand at 745.667 kb on + strand at 745.667 kb on + strand at 745.668 kb on - strand at 745.668 kb on - strand at 745.668 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 742,528 + GFF687 0.73 -0.4 742,528 + GFF687 0.73 +0.3 742,528 + GFF687 0.73 -0.5 742,528 + GFF687 0.73 +0.4 742,529 - GFF687 0.73 -0.1 742,529 - GFF687 0.73 +0.9 742,529 - GFF687 0.73 +0.7 742,529 - GFF687 0.73 +0.0 742,529 - GFF687 0.73 -0.6 742,547 - GFF687 0.74 -1.4 742,711 + GFF687 0.84 -0.0 742,711 + GFF687 0.84 +0.6 742,711 + GFF687 0.84 +1.7 742,711 + GFF687 0.84 +0.4 742,711 + GFF687 0.84 -0.3 742,711 + GFF687 0.84 -2.3 742,712 - GFF687 0.84 -0.2 742,712 - GFF687 0.84 -1.4 742,948 + +0.5 742,948 + +0.2 742,948 + +0.4 742,948 + -0.1 742,948 + -0.2 742,949 - -0.1 742,949 - -0.0 743,441 + -0.7 743,442 - -1.7 743,442 - +1.6 743,531 + +0.2 743,531 + -0.9 743,531 + +0.4 743,771 + GFF689 0.26 -1.5 743,772 - GFF689 0.26 -0.6 743,772 - GFF689 0.26 -0.5 743,772 - GFF689 0.26 -0.3 743,772 - GFF689 0.26 -0.1 743,772 - GFF689 0.26 -0.6 743,772 - GFF689 0.26 -0.6 743,772 - GFF689 0.26 +0.7 743,795 + GFF689 0.28 -1.7 743,795 + GFF689 0.28 +0.0 743,795 + GFF689 0.28 -3.1 743,795 + GFF689 0.28 -0.4 743,795 + GFF689 0.28 -0.7 743,796 - GFF689 0.28 +0.4 743,796 - GFF689 0.28 +0.9 743,837 + GFF689 0.31 -0.6 743,838 - GFF689 0.31 -1.0 743,865 - GFF689 0.33 +0.3 743,945 + GFF689 0.39 -1.3 743,945 + GFF689 0.39 -0.7 743,945 + GFF689 0.39 -1.1 743,945 + GFF689 0.39 -1.1 743,945 + GFF689 0.39 -1.9 743,945 + GFF689 0.39 -0.6 743,945 + GFF689 0.39 +0.7 743,945 + GFF689 0.39 +0.1 743,945 + GFF689 0.39 -0.7 743,945 + GFF689 0.39 -2.1 743,945 + GFF689 0.39 +0.4 743,945 + GFF689 0.39 -0.3 743,946 - GFF689 0.40 -1.7 743,946 - GFF689 0.40 -0.1 743,946 - GFF689 0.40 +1.0 743,946 - GFF689 0.40 -1.4 743,946 - GFF689 0.40 +0.4 743,946 - GFF689 0.40 -0.0 744,078 - GFF689 0.50 -0.9 744,174 - GFF689 0.57 +0.1 744,257 + GFF689 0.64 +0.6 744,257 + GFF689 0.64 +1.9 744,257 + GFF689 0.64 -1.6 744,257 + GFF689 0.64 -0.4 744,257 + GFF689 0.64 +1.0 744,257 + GFF689 0.64 +0.3 744,257 + GFF689 0.64 -0.1 744,258 - GFF689 0.64 +0.1 744,258 - GFF689 0.64 -0.6 744,258 - GFF689 0.64 -0.7 744,258 - GFF689 0.64 -0.9 744,258 - GFF689 0.64 +0.4 744,258 - GFF689 0.64 -1.1 744,258 - GFF689 0.64 +0.1 744,323 + GFF689 0.69 -0.1 744,323 + GFF689 0.69 +0.4 744,324 - GFF689 0.69 -1.1 744,386 + GFF689 0.73 +0.2 744,386 + GFF689 0.73 -0.4 744,386 + GFF689 0.73 +1.0 744,386 + GFF689 0.73 +0.2 744,644 + -0.3 744,645 - -1.6 744,698 + -0.7 744,698 + -0.4 744,698 + -0.4 744,698 + -0.6 744,699 - +0.8 744,699 - +0.5 744,699 - +1.0 744,699 - -0.7 744,699 - +1.4 744,699 - +0.1 744,699 - -1.1 744,787 + -1.0 744,788 - +0.2 744,788 - -0.5 744,807 + -0.2 744,807 + -0.4 744,808 - -1.0 745,003 + GFF690 0.26 +0.4 745,132 + GFF690 0.41 +0.0 745,132 + GFF690 0.41 -0.8 745,133 - GFF690 0.41 -1.6 745,133 - GFF690 0.41 +0.4 745,133 - GFF690 0.41 -0.4 745,133 - GFF690 0.41 -1.6 745,237 + GFF690 0.53 +0.3 745,262 - GFF690 0.56 -0.8 745,262 - GFF690 0.56 -0.4 745,667 + -0.9 745,667 + +0.1 745,667 + -0.2 745,667 + -1.6 745,667 + +0.2 745,668 - +0.0 745,668 - +1.0 745,668 - -0.1
Or see this region's nucleotide sequence