Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6867 and GFF6868 overlap by 4 nucleotides GFF6868 and GFF6869 overlap by 11 nucleotides
GFF6867 - no description, at 75 to 755
GFF6867
GFF6868 - SAM-dependent methyltransferase, at 752 to 1,504
GFF6868
GFF6869 - no description, at 1,494 to 1,964
GFF6869
Position (kb)
0
1 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 0.088 kb on + strand at 0.088 kb on + strand at 0.088 kb on + strand at 0.088 kb on + strand at 0.088 kb on + strand at 0.088 kb on + strand at 0.088 kb on + strand at 0.088 kb on + strand at 0.089 kb on - strand at 0.089 kb on - strand at 0.089 kb on - strand at 0.089 kb on - strand at 0.089 kb on - strand at 0.142 kb on + strand at 0.143 kb on - strand, within GFF6867 at 0.143 kb on - strand, within GFF6867 at 0.178 kb on + strand, within GFF6867 at 0.179 kb on - strand, within GFF6867 at 0.200 kb on - strand, within GFF6867 at 0.341 kb on - strand, within GFF6867 at 0.341 kb on - strand, within GFF6867 at 0.341 kb on - strand, within GFF6867 at 0.341 kb on - strand, within GFF6867 at 0.356 kb on - strand, within GFF6867 at 0.356 kb on - strand, within GFF6867 at 0.597 kb on + strand, within GFF6867 at 0.597 kb on + strand, within GFF6867 at 0.597 kb on + strand, within GFF6867 at 0.597 kb on + strand, within GFF6867 at 0.597 kb on + strand, within GFF6867 at 0.597 kb on + strand, within GFF6867 at 0.597 kb on + strand, within GFF6867 at 0.597 kb on + strand, within GFF6867 at 0.598 kb on - strand, within GFF6867 at 0.598 kb on - strand, within GFF6867 at 0.598 kb on - strand, within GFF6867 at 0.649 kb on + strand, within GFF6867 at 0.649 kb on + strand, within GFF6867 at 0.650 kb on - strand, within GFF6867 at 0.650 kb on - strand, within GFF6867 at 0.650 kb on - strand, within GFF6867 at 0.697 kb on + strand at 0.697 kb on + strand at 0.697 kb on + strand at 0.697 kb on + strand at 0.698 kb on - strand at 0.727 kb on + strand at 0.728 kb on - strand at 0.728 kb on - strand at 0.728 kb on - strand at 0.825 kb on + strand at 0.825 kb on + strand at 0.825 kb on + strand at 0.825 kb on + strand at 0.825 kb on + strand at 0.826 kb on - strand at 0.826 kb on - strand at 0.826 kb on - strand at 0.840 kb on + strand, within GFF6868 at 0.840 kb on + strand, within GFF6868 at 0.841 kb on - strand, within GFF6868 at 0.841 kb on - strand, within GFF6868 at 0.841 kb on - strand, within GFF6868 at 0.965 kb on - strand, within GFF6868 at 0.975 kb on + strand, within GFF6868 at 0.976 kb on - strand, within GFF6868 at 0.976 kb on - strand, within GFF6868 at 0.976 kb on - strand, within GFF6868 at 0.976 kb on - strand, within GFF6868 at 1.194 kb on + strand, within GFF6868 at 1.195 kb on - strand, within GFF6868 at 1.195 kb on - strand, within GFF6868 at 1.317 kb on + strand, within GFF6868 at 1.318 kb on - strand, within GFF6868 at 1.318 kb on - strand, within GFF6868 at 1.318 kb on - strand, within GFF6868 at 1.318 kb on - strand, within GFF6868 at 1.318 kb on - strand, within GFF6868 at 1.413 kb on + strand, within GFF6868 at 1.413 kb on + strand, within GFF6868 at 1.413 kb on + strand, within GFF6868 at 1.413 kb on + strand, within GFF6868 at 1.413 kb on + strand, within GFF6868 at 1.413 kb on - strand, within GFF6868 at 1.414 kb on - strand, within GFF6868 at 1.414 kb on - strand, within GFF6868 at 1.414 kb on - strand, within GFF6868 at 1.414 kb on - strand, within GFF6868 at 1.414 kb on - strand, within GFF6868 at 1.443 kb on + strand at 1.443 kb on + strand at 1.443 kb on + strand at 1.443 kb on + strand at 1.443 kb on + strand at 1.449 kb on + strand at 1.449 kb on + strand at 1.450 kb on - strand at 1.485 kb on + strand at 1.485 kb on + strand at 1.485 kb on + strand at 1.486 kb on - strand at 1.539 kb on + strand at 1.539 kb on + strand at 1.539 kb on + strand at 1.539 kb on + strand at 1.539 kb on + strand at 1.539 kb on + strand at 1.539 kb on + strand at 1.539 kb on + strand at 1.539 kb on + strand at 1.539 kb on + strand at 1.539 kb on + strand at 1.540 kb on - strand at 1.540 kb on - strand at 1.540 kb on - strand at 1.540 kb on - strand at 1.540 kb on - strand at 1.540 kb on - strand at 1.540 kb on - strand at 1.540 kb on - strand at 1.540 kb on - strand at 1.540 kb on - strand at 1.540 kb on - strand at 1.585 kb on + strand, within GFF6869 at 1.607 kb on + strand, within GFF6869 at 1.608 kb on - strand, within GFF6869 at 1.608 kb on - strand, within GFF6869 at 1.608 kb on - strand, within GFF6869 at 1.608 kb on - strand, within GFF6869 at 1.608 kb on - strand, within GFF6869 at 1.610 kb on + strand, within GFF6869 at 1.610 kb on + strand, within GFF6869 at 1.610 kb on + strand, within GFF6869 at 1.610 kb on + strand, within GFF6869 at 1.610 kb on + strand, within GFF6869 at 1.707 kb on + strand, within GFF6869 at 1.708 kb on - strand, within GFF6869 at 1.717 kb on - strand, within GFF6869
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 88 + -0.3 88 + +0.6 88 + -0.6 88 + -0.9 88 + -1.3 88 + -0.2 88 + +0.5 88 + -0.6 89 - -1.0 89 - +1.5 89 - +0.7 89 - -0.0 89 - -0.8 142 + +0.7 143 - GFF6867 0.10 -1.5 143 - GFF6867 0.10 -1.0 178 + GFF6867 0.15 -0.2 179 - GFF6867 0.15 +0.6 200 - GFF6867 0.18 -0.8 341 - GFF6867 0.39 -0.2 341 - GFF6867 0.39 -1.0 341 - GFF6867 0.39 +0.3 341 - GFF6867 0.39 -1.6 356 - GFF6867 0.41 +1.0 356 - GFF6867 0.41 -0.7 597 + GFF6867 0.77 +0.4 597 + GFF6867 0.77 -0.3 597 + GFF6867 0.77 -0.1 597 + GFF6867 0.77 -0.8 597 + GFF6867 0.77 -0.2 597 + GFF6867 0.77 -1.6 597 + GFF6867 0.77 +0.2 597 + GFF6867 0.77 -0.6 598 - GFF6867 0.77 -1.0 598 - GFF6867 0.77 -0.1 598 - GFF6867 0.77 -0.5 649 + GFF6867 0.84 -0.7 649 + GFF6867 0.84 +2.0 650 - GFF6867 0.84 -0.1 650 - GFF6867 0.84 -0.1 650 - GFF6867 0.84 -0.0 697 + -0.8 697 + +1.6 697 + -0.8 697 + -2.7 698 - -1.0 727 + +0.2 728 - +0.4 728 - +0.2 728 - +0.9 825 + -1.0 825 + +1.1 825 + -1.7 825 + -0.1 825 + +0.7 826 - +1.0 826 - -0.6 826 - -0.8 840 + GFF6868 0.12 -1.2 840 + GFF6868 0.12 +1.4 841 - GFF6868 0.12 -0.7 841 - GFF6868 0.12 +1.0 841 - GFF6868 0.12 -0.4 965 - GFF6868 0.28 -1.2 975 + GFF6868 0.30 +0.5 976 - GFF6868 0.30 -0.0 976 - GFF6868 0.30 -0.6 976 - GFF6868 0.30 -1.0 976 - GFF6868 0.30 -2.2 1,194 + GFF6868 0.59 -1.9 1,195 - GFF6868 0.59 -0.0 1,195 - GFF6868 0.59 -0.5 1,317 + GFF6868 0.75 -1.0 1,318 - GFF6868 0.75 -0.2 1,318 - GFF6868 0.75 -1.5 1,318 - GFF6868 0.75 +3.0 1,318 - GFF6868 0.75 -0.5 1,318 - GFF6868 0.75 +0.2 1,413 + GFF6868 0.88 -1.0 1,413 + GFF6868 0.88 -0.0 1,413 + GFF6868 0.88 -2.9 1,413 + GFF6868 0.88 -0.0 1,413 + GFF6868 0.88 +0.2 1,413 - GFF6868 0.88 -0.0 1,414 - GFF6868 0.88 +1.3 1,414 - GFF6868 0.88 -0.3 1,414 - GFF6868 0.88 -0.0 1,414 - GFF6868 0.88 -0.0 1,414 - GFF6868 0.88 +0.4 1,443 + +1.0 1,443 + -1.0 1,443 + -1.9 1,443 + -2.4 1,443 + -0.6 1,449 + -1.0 1,449 + -1.2 1,450 - -0.5 1,485 + +0.4 1,485 + +0.1 1,485 + +0.4 1,486 - +1.0 1,539 + -1.4 1,539 + -0.8 1,539 + +0.8 1,539 + -0.8 1,539 + -0.7 1,539 + -0.6 1,539 + -2.1 1,539 + +1.4 1,539 + -0.6 1,539 + +0.6 1,539 + +0.1 1,540 - +0.8 1,540 - +0.2 1,540 - -1.2 1,540 - -0.1 1,540 - +0.7 1,540 - +0.2 1,540 - -0.6 1,540 - +2.0 1,540 - +0.4 1,540 - -1.7 1,540 - -0.6 1,585 + GFF6869 0.19 -0.6 1,607 + GFF6869 0.24 -0.8 1,608 - GFF6869 0.24 +0.5 1,608 - GFF6869 0.24 -1.2 1,608 - GFF6869 0.24 +2.6 1,608 - GFF6869 0.24 +1.0 1,608 - GFF6869 0.24 +1.0 1,610 + GFF6869 0.25 -0.3 1,610 + GFF6869 0.25 -0.6 1,610 + GFF6869 0.25 -1.0 1,610 + GFF6869 0.25 -1.5 1,610 + GFF6869 0.25 -1.1 1,707 + GFF6869 0.45 -1.5 1,708 - GFF6869 0.45 -0.5 1,717 - GFF6869 0.47 -1.1
Or see this region's nucleotide sequence