Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6799 and GFF6800 are separated by 15 nucleotides GFF6800 and GFF6801 overlap by 4 nucleotides GFF6801 and GFF6802 are separated by 97 nucleotides
GFF6799 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 46,395 to 47,057
GFF6799
GFF6800 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 47,073 to 47,876
GFF6800
GFF6801 - Transcriptional regulator, GntR family, at 47,873 to 48,664
GFF6801
GFF6802 - Uncharacterized MFS-type transporter, at 48,762 to 50,102
GFF6802
Position (kb)
47
48
49 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 46.879 kb on + strand, within GFF6799 at 46.879 kb on + strand, within GFF6799 at 46.879 kb on + strand, within GFF6799 at 46.879 kb on + strand, within GFF6799 at 46.879 kb on + strand, within GFF6799 at 46.879 kb on + strand, within GFF6799 at 46.880 kb on - strand, within GFF6799 at 46.880 kb on - strand, within GFF6799 at 46.880 kb on - strand, within GFF6799 at 46.880 kb on - strand, within GFF6799 at 46.880 kb on - strand, within GFF6799 at 46.880 kb on - strand, within GFF6799 at 47.047 kb on + strand at 47.365 kb on + strand, within GFF6800 at 47.365 kb on + strand, within GFF6800 at 47.365 kb on + strand, within GFF6800 at 47.485 kb on + strand, within GFF6800 at 47.485 kb on + strand, within GFF6800 at 47.486 kb on - strand, within GFF6800 at 47.596 kb on + strand, within GFF6800 at 47.876 kb on + strand at 47.876 kb on + strand at 47.876 kb on + strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.877 kb on - strand at 47.888 kb on + strand at 47.888 kb on + strand at 47.888 kb on + strand at 47.888 kb on + strand at 47.889 kb on - strand at 47.889 kb on - strand at 47.889 kb on - strand at 47.889 kb on - strand at 47.889 kb on - strand at 47.889 kb on - strand at 47.892 kb on + strand at 47.892 kb on + strand at 47.892 kb on + strand at 47.892 kb on + strand at 47.893 kb on - strand at 47.893 kb on - strand at 48.134 kb on + strand, within GFF6801 at 48.134 kb on + strand, within GFF6801 at 48.134 kb on + strand, within GFF6801 at 48.134 kb on + strand, within GFF6801 at 48.134 kb on + strand, within GFF6801 at 48.134 kb on + strand, within GFF6801 at 48.134 kb on + strand, within GFF6801 at 48.134 kb on + strand, within GFF6801 at 48.134 kb on + strand, within GFF6801 at 48.134 kb on + strand, within GFF6801 at 48.134 kb on + strand, within GFF6801 at 48.135 kb on - strand, within GFF6801 at 48.135 kb on - strand, within GFF6801 at 48.135 kb on - strand, within GFF6801 at 48.135 kb on - strand, within GFF6801 at 48.135 kb on - strand, within GFF6801 at 48.135 kb on - strand, within GFF6801 at 48.135 kb on - strand, within GFF6801 at 48.135 kb on - strand, within GFF6801 at 48.135 kb on - strand, within GFF6801 at 48.135 kb on - strand, within GFF6801 at 48.135 kb on - strand, within GFF6801 at 48.135 kb on - strand, within GFF6801 at 48.135 kb on - strand, within GFF6801 at 48.177 kb on + strand, within GFF6801 at 48.178 kb on - strand, within GFF6801 at 48.178 kb on - strand, within GFF6801 at 48.178 kb on - strand, within GFF6801 at 48.210 kb on + strand, within GFF6801 at 48.210 kb on + strand, within GFF6801 at 48.211 kb on - strand, within GFF6801 at 48.211 kb on - strand, within GFF6801 at 48.211 kb on - strand, within GFF6801 at 48.394 kb on + strand, within GFF6801 at 48.563 kb on + strand, within GFF6801 at 48.564 kb on - strand, within GFF6801 at 48.564 kb on - strand, within GFF6801 at 48.664 kb on + strand at 48.664 kb on + strand at 48.665 kb on - strand at 48.665 kb on - strand at 48.665 kb on - strand at 48.674 kb on + strand at 48.674 kb on + strand at 48.675 kb on - strand at 48.675 kb on - strand at 48.699 kb on + strand at 48.699 kb on + strand at 48.843 kb on + strand at 48.843 kb on + strand at 48.844 kb on - strand at 48.847 kb on + strand at 48.848 kb on - strand at 48.848 kb on - strand at 48.848 kb on - strand at 48.848 kb on - strand at 48.848 kb on - strand at 48.848 kb on - strand at 49.210 kb on + strand, within GFF6802 at 49.210 kb on + strand, within GFF6802 at 49.280 kb on - strand, within GFF6802 at 49.280 kb on - strand, within GFF6802 at 49.280 kb on - strand, within GFF6802
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 46,879 + GFF6799 0.73 -1.4 46,879 + GFF6799 0.73 -0.5 46,879 + GFF6799 0.73 +1.6 46,879 + GFF6799 0.73 +0.5 46,879 + GFF6799 0.73 -0.2 46,879 + GFF6799 0.73 -0.4 46,880 - GFF6799 0.73 -0.5 46,880 - GFF6799 0.73 +1.3 46,880 - GFF6799 0.73 +0.6 46,880 - GFF6799 0.73 +0.1 46,880 - GFF6799 0.73 -0.3 46,880 - GFF6799 0.73 +0.5 47,047 + -0.9 47,365 + GFF6800 0.36 -0.9 47,365 + GFF6800 0.36 -0.5 47,365 + GFF6800 0.36 +0.1 47,485 + GFF6800 0.51 -0.2 47,485 + GFF6800 0.51 +0.4 47,486 - GFF6800 0.51 -0.5 47,596 + GFF6800 0.65 -0.3 47,876 + -0.2 47,876 + -1.4 47,876 + -0.3 47,877 - +0.2 47,877 - -0.7 47,877 - +0.1 47,877 - -1.1 47,877 - +0.5 47,877 - -1.2 47,877 - -0.4 47,877 - -1.7 47,877 - -0.6 47,877 - -0.5 47,877 - -0.2 47,877 - -0.2 47,888 + -0.7 47,888 + +0.0 47,888 + +0.2 47,888 + -0.9 47,889 - +0.2 47,889 - +0.8 47,889 - -0.1 47,889 - -0.8 47,889 - -0.4 47,889 - -0.8 47,892 + -0.2 47,892 + +1.1 47,892 + +0.2 47,892 + -1.4 47,893 - -1.4 47,893 - +0.5 48,134 + GFF6801 0.33 -1.8 48,134 + GFF6801 0.33 -1.1 48,134 + GFF6801 0.33 -0.8 48,134 + GFF6801 0.33 -0.3 48,134 + GFF6801 0.33 -0.5 48,134 + GFF6801 0.33 +1.1 48,134 + GFF6801 0.33 -0.6 48,134 + GFF6801 0.33 +1.3 48,134 + GFF6801 0.33 -1.2 48,134 + GFF6801 0.33 -0.4 48,134 + GFF6801 0.33 -0.5 48,135 - GFF6801 0.33 -0.4 48,135 - GFF6801 0.33 -0.0 48,135 - GFF6801 0.33 -0.0 48,135 - GFF6801 0.33 +0.6 48,135 - GFF6801 0.33 +0.6 48,135 - GFF6801 0.33 -1.6 48,135 - GFF6801 0.33 +0.1 48,135 - GFF6801 0.33 -1.2 48,135 - GFF6801 0.33 +0.3 48,135 - GFF6801 0.33 +1.6 48,135 - GFF6801 0.33 -0.4 48,135 - GFF6801 0.33 -0.4 48,135 - GFF6801 0.33 +0.1 48,177 + GFF6801 0.38 -0.7 48,178 - GFF6801 0.39 -1.1 48,178 - GFF6801 0.39 -1.3 48,178 - GFF6801 0.39 -0.7 48,210 + GFF6801 0.43 -0.5 48,210 + GFF6801 0.43 +0.3 48,211 - GFF6801 0.43 -1.8 48,211 - GFF6801 0.43 -0.8 48,211 - GFF6801 0.43 -0.3 48,394 + GFF6801 0.66 -0.2 48,563 + GFF6801 0.87 -0.9 48,564 - GFF6801 0.87 -0.6 48,564 - GFF6801 0.87 -0.9 48,664 + +0.1 48,664 + -0.9 48,665 - +1.3 48,665 - +1.1 48,665 - +0.8 48,674 + -0.7 48,674 + +2.1 48,675 - -1.2 48,675 - +1.9 48,699 + -0.3 48,699 + +0.1 48,843 + -0.1 48,843 + -0.7 48,844 - -0.2 48,847 + +1.3 48,848 - +0.9 48,848 - -1.4 48,848 - -0.2 48,848 - +0.6 48,848 - +0.4 48,848 - -0.8 49,210 + GFF6802 0.33 +0.2 49,210 + GFF6802 0.33 +0.8 49,280 - GFF6802 0.39 -0.2 49,280 - GFF6802 0.39 -1.7 49,280 - GFF6802 0.39 -0.1
Or see this region's nucleotide sequence