Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6617 and GFF6618 are separated by 79 nucleotides GFF6618 and GFF6619 are separated by 508 nucleotides GFF6619 and GFF6620 are separated by 42 nucleotides
GFF6617 - no description, at 61,882 to 62,058
GFF6617
GFF6618 - Mlr6156 protein, at 62,138 to 62,494
GFF6618
GFF6619 - hypothetical protein, at 63,003 to 64,163
GFF6619
GFF6620 - no description, at 64,206 to 65,174
GFF6620
Position (kb) 63
64
65 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 62.178 kb on + strand, within GFF6618 at 62.208 kb on + strand, within GFF6618 at 62.209 kb on - strand, within GFF6618 at 62.209 kb on - strand, within GFF6618 at 62.685 kb on + strand at 62.686 kb on - strand at 62.858 kb on - strand at 62.905 kb on + strand at 62.906 kb on - strand at 62.921 kb on + strand at 62.922 kb on - strand at 62.922 kb on - strand at 63.008 kb on + strand at 63.008 kb on + strand at 63.008 kb on + strand at 63.009 kb on - strand at 63.009 kb on - strand at 63.009 kb on - strand at 63.009 kb on - strand at 63.167 kb on - strand, within GFF6619 at 63.355 kb on - strand, within GFF6619 at 63.355 kb on - strand, within GFF6619 at 63.355 kb on - strand, within GFF6619 at 63.355 kb on - strand, within GFF6619 at 63.355 kb on - strand, within GFF6619 at 63.396 kb on - strand, within GFF6619 at 63.435 kb on + strand, within GFF6619 at 63.436 kb on - strand, within GFF6619 at 63.536 kb on + strand, within GFF6619 at 63.536 kb on + strand, within GFF6619 at 63.536 kb on + strand, within GFF6619 at 63.536 kb on + strand, within GFF6619 at 63.537 kb on - strand, within GFF6619 at 63.537 kb on - strand, within GFF6619 at 63.573 kb on + strand, within GFF6619 at 63.573 kb on + strand, within GFF6619 at 63.573 kb on + strand, within GFF6619 at 63.573 kb on + strand, within GFF6619 at 63.573 kb on + strand, within GFF6619 at 63.573 kb on + strand, within GFF6619 at 63.574 kb on - strand, within GFF6619 at 63.574 kb on - strand, within GFF6619 at 63.574 kb on - strand, within GFF6619 at 63.574 kb on - strand, within GFF6619 at 64.088 kb on + strand at 64.088 kb on + strand at 64.193 kb on + strand at 64.193 kb on + strand at 64.193 kb on + strand at 64.193 kb on + strand at 64.193 kb on + strand at 64.193 kb on + strand at 64.193 kb on + strand at 64.193 kb on + strand at 64.193 kb on + strand at 64.193 kb on + strand at 64.193 kb on + strand at 64.193 kb on + strand at 64.193 kb on + strand at 64.194 kb on - strand at 64.194 kb on - strand at 64.194 kb on - strand at 64.194 kb on - strand at 64.194 kb on - strand at 64.194 kb on - strand at 64.194 kb on - strand at 64.194 kb on - strand at 64.194 kb on - strand at 64.194 kb on - strand at 64.194 kb on - strand at 64.241 kb on + strand at 64.241 kb on + strand at 64.242 kb on - strand at 64.334 kb on + strand, within GFF6620 at 64.380 kb on + strand, within GFF6620 at 64.380 kb on + strand, within GFF6620 at 64.380 kb on + strand, within GFF6620 at 64.380 kb on + strand, within GFF6620 at 64.381 kb on - strand, within GFF6620 at 64.396 kb on + strand, within GFF6620 at 64.397 kb on - strand, within GFF6620 at 64.615 kb on - strand, within GFF6620 at 64.741 kb on + strand, within GFF6620 at 64.741 kb on + strand, within GFF6620 at 64.957 kb on + strand, within GFF6620 at 64.995 kb on + strand, within GFF6620 at 64.996 kb on - strand, within GFF6620 at 64.996 kb on - strand, within GFF6620 at 64.996 kb on - strand, within GFF6620 at 65.004 kb on + strand, within GFF6620 at 65.004 kb on + strand, within GFF6620 at 65.101 kb on + strand at 65.102 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 62,178 + GFF6618 0.11 +0.3 62,208 + GFF6618 0.20 -0.4 62,209 - GFF6618 0.20 -1.4 62,209 - GFF6618 0.20 -0.9 62,685 + -0.1 62,686 - -0.2 62,858 - -0.4 62,905 + +0.8 62,906 - +0.2 62,921 + -0.4 62,922 - +0.2 62,922 - -0.4 63,008 + -0.7 63,008 + -1.2 63,008 + -1.2 63,009 - +0.4 63,009 - -0.0 63,009 - -0.6 63,009 - -0.4 63,167 - GFF6619 0.14 -1.4 63,355 - GFF6619 0.30 +0.2 63,355 - GFF6619 0.30 -1.7 63,355 - GFF6619 0.30 -1.0 63,355 - GFF6619 0.30 +0.6 63,355 - GFF6619 0.30 +0.5 63,396 - GFF6619 0.34 +0.4 63,435 + GFF6619 0.37 -0.5 63,436 - GFF6619 0.37 -0.6 63,536 + GFF6619 0.46 -1.0 63,536 + GFF6619 0.46 -0.6 63,536 + GFF6619 0.46 -0.0 63,536 + GFF6619 0.46 -0.1 63,537 - GFF6619 0.46 -0.8 63,537 - GFF6619 0.46 -0.1 63,573 + GFF6619 0.49 -0.6 63,573 + GFF6619 0.49 -1.1 63,573 + GFF6619 0.49 -0.5 63,573 + GFF6619 0.49 -0.7 63,573 + GFF6619 0.49 -0.3 63,573 + GFF6619 0.49 +1.3 63,574 - GFF6619 0.49 -0.7 63,574 - GFF6619 0.49 +1.2 63,574 - GFF6619 0.49 -0.5 63,574 - GFF6619 0.49 -0.0 64,088 + -1.1 64,088 + +0.7 64,193 + -1.0 64,193 + +0.2 64,193 + -0.8 64,193 + +0.1 64,193 + -1.5 64,193 + +0.3 64,193 + -0.4 64,193 + -0.2 64,193 + -0.2 64,193 + -0.6 64,193 + -1.3 64,193 + -0.8 64,193 + -0.2 64,194 - -0.6 64,194 - -0.5 64,194 - +0.3 64,194 - +0.9 64,194 - +0.0 64,194 - -0.1 64,194 - -1.0 64,194 - -2.8 64,194 - -0.4 64,194 - -2.1 64,194 - +0.2 64,241 + +0.5 64,241 + +0.7 64,242 - +0.6 64,334 + GFF6620 0.13 +0.5 64,380 + GFF6620 0.18 -0.8 64,380 + GFF6620 0.18 +2.4 64,380 + GFF6620 0.18 -1.8 64,380 + GFF6620 0.18 +1.6 64,381 - GFF6620 0.18 -1.2 64,396 + GFF6620 0.20 -2.4 64,397 - GFF6620 0.20 -1.6 64,615 - GFF6620 0.42 +1.3 64,741 + GFF6620 0.55 +0.0 64,741 + GFF6620 0.55 -0.2 64,957 + GFF6620 0.78 -0.6 64,995 + GFF6620 0.81 +1.8 64,996 - GFF6620 0.82 -0.0 64,996 - GFF6620 0.82 -0.7 64,996 - GFF6620 0.82 +0.2 65,004 + GFF6620 0.82 +0.3 65,004 + GFF6620 0.82 +0.8 65,101 + -1.4 65,102 - -1.7
Or see this region's nucleotide sequence