Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6329 and GFF6330 are separated by 148 nucleotides GFF6330 and GFF6331 overlap by 8 nucleotides
GFF6329 - Leucyl-tRNA synthetase (EC 6.1.1.4), at 121,832 to 124,462
GFF6329
GFF6330 - BUG/TctC family periplasmic protein, at 124,611 to 125,585
GFF6330
GFF6331 - D-octopine dehydrogenase (EC 1.5.1.11), at 125,578 to 126,648
GFF6331
Position (kb)
124
125
126 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 124.546 kb on - strand at 124.546 kb on - strand at 124.560 kb on + strand at 124.560 kb on + strand at 124.560 kb on + strand at 124.560 kb on + strand at 124.561 kb on - strand at 124.561 kb on - strand at 124.561 kb on - strand at 124.561 kb on - strand at 124.935 kb on + strand, within GFF6330 at 124.935 kb on + strand, within GFF6330 at 124.936 kb on - strand, within GFF6330 at 124.936 kb on - strand, within GFF6330 at 124.936 kb on - strand, within GFF6330 at 125.016 kb on + strand, within GFF6330 at 125.016 kb on + strand, within GFF6330 at 125.017 kb on - strand, within GFF6330 at 125.073 kb on + strand, within GFF6330 at 125.073 kb on + strand, within GFF6330 at 125.073 kb on + strand, within GFF6330 at 125.073 kb on + strand, within GFF6330 at 125.073 kb on + strand, within GFF6330 at 125.073 kb on + strand, within GFF6330 at 125.074 kb on - strand, within GFF6330 at 125.074 kb on - strand, within GFF6330 at 125.074 kb on - strand, within GFF6330 at 125.074 kb on - strand, within GFF6330 at 125.074 kb on - strand, within GFF6330 at 125.074 kb on - strand, within GFF6330 at 125.074 kb on - strand, within GFF6330 at 125.583 kb on + strand at 125.584 kb on - strand at 125.584 kb on - strand at 125.584 kb on - strand at 125.593 kb on + strand at 125.593 kb on + strand at 125.594 kb on - strand at 125.594 kb on - strand at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.807 kb on + strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.808 kb on - strand, within GFF6331 at 125.920 kb on + strand, within GFF6331 at 125.920 kb on + strand, within GFF6331 at 125.920 kb on + strand, within GFF6331 at 125.920 kb on + strand, within GFF6331 at 125.920 kb on + strand, within GFF6331 at 125.921 kb on - strand, within GFF6331 at 125.921 kb on - strand, within GFF6331 at 125.921 kb on - strand, within GFF6331 at 125.921 kb on - strand, within GFF6331 at 125.921 kb on - strand, within GFF6331 at 126.069 kb on + strand, within GFF6331 at 126.070 kb on - strand, within GFF6331 at 126.235 kb on + strand, within GFF6331 at 126.236 kb on - strand, within GFF6331 at 126.318 kb on - strand, within GFF6331 at 126.318 kb on - strand, within GFF6331 at 126.448 kb on + strand, within GFF6331 at 126.448 kb on + strand, within GFF6331 at 126.448 kb on + strand, within GFF6331 at 126.449 kb on - strand, within GFF6331 at 126.449 kb on - strand, within GFF6331 at 126.449 kb on - strand, within GFF6331 at 126.449 kb on - strand, within GFF6331 at 126.469 kb on + strand, within GFF6331 at 126.469 kb on + strand, within GFF6331
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 124,546 - -0.4 124,546 - -0.5 124,560 + -0.1 124,560 + +1.0 124,560 + -0.3 124,560 + -0.4 124,561 - +1.1 124,561 - -0.7 124,561 - -0.2 124,561 - -0.2 124,935 + GFF6330 0.33 +0.8 124,935 + GFF6330 0.33 +0.6 124,936 - GFF6330 0.33 -0.1 124,936 - GFF6330 0.33 +1.7 124,936 - GFF6330 0.33 +0.5 125,016 + GFF6330 0.42 +0.9 125,016 + GFF6330 0.42 +0.3 125,017 - GFF6330 0.42 +1.1 125,073 + GFF6330 0.47 +0.3 125,073 + GFF6330 0.47 -0.8 125,073 + GFF6330 0.47 +1.4 125,073 + GFF6330 0.47 -0.6 125,073 + GFF6330 0.47 +0.1 125,073 + GFF6330 0.47 +0.2 125,074 - GFF6330 0.47 -0.8 125,074 - GFF6330 0.47 -1.3 125,074 - GFF6330 0.47 -1.2 125,074 - GFF6330 0.47 -1.6 125,074 - GFF6330 0.47 -0.7 125,074 - GFF6330 0.47 +1.4 125,074 - GFF6330 0.47 -0.3 125,583 + +0.1 125,584 - +0.8 125,584 - -1.7 125,584 - -2.9 125,593 + +0.3 125,593 + +1.2 125,594 - +0.6 125,594 - +0.1 125,807 + GFF6331 0.21 -0.1 125,807 + GFF6331 0.21 -0.7 125,807 + GFF6331 0.21 +0.5 125,807 + GFF6331 0.21 +0.3 125,807 + GFF6331 0.21 +0.1 125,807 + GFF6331 0.21 -1.3 125,807 + GFF6331 0.21 +0.3 125,807 + GFF6331 0.21 -0.6 125,808 - GFF6331 0.21 -1.0 125,808 - GFF6331 0.21 +0.0 125,808 - GFF6331 0.21 -0.6 125,808 - GFF6331 0.21 +0.3 125,808 - GFF6331 0.21 -0.5 125,808 - GFF6331 0.21 +0.0 125,808 - GFF6331 0.21 +0.3 125,920 + GFF6331 0.32 +0.4 125,920 + GFF6331 0.32 +0.1 125,920 + GFF6331 0.32 -0.1 125,920 + GFF6331 0.32 -0.3 125,920 + GFF6331 0.32 -1.1 125,921 - GFF6331 0.32 -0.4 125,921 - GFF6331 0.32 -1.6 125,921 - GFF6331 0.32 -0.1 125,921 - GFF6331 0.32 +0.0 125,921 - GFF6331 0.32 -1.0 126,069 + GFF6331 0.46 +1.0 126,070 - GFF6331 0.46 +1.0 126,235 + GFF6331 0.61 +0.5 126,236 - GFF6331 0.61 +0.1 126,318 - GFF6331 0.69 +0.0 126,318 - GFF6331 0.69 +0.0 126,448 + GFF6331 0.81 +1.0 126,448 + GFF6331 0.81 -0.1 126,448 + GFF6331 0.81 +0.7 126,449 - GFF6331 0.81 +1.0 126,449 - GFF6331 0.81 +0.3 126,449 - GFF6331 0.81 +0.2 126,449 - GFF6331 0.81 -0.8 126,469 + GFF6331 0.83 -0.6 126,469 + GFF6331 0.83 -0.7
Or see this region's nucleotide sequence