Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6235 and GFF6236 are separated by 390 nucleotides GFF6236 and GFF6237 overlap by 1 nucleotides
GFF6235 - hypothetical protein, at 23,581 to 25,701
GFF6235
GFF6236 - VgrG protein, at 26,092 to 28,746
GFF6236
GFF6237 - hypothetical protein, at 28,746 to 29,813
GFF6237
Position (kb) 26
27
28
29 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 25.146 kb on - strand, within GFF6235 at 25.178 kb on + strand, within GFF6235 at 25.178 kb on + strand, within GFF6235 at 25.179 kb on - strand, within GFF6235 at 25.179 kb on - strand, within GFF6235 at 25.179 kb on - strand, within GFF6235 at 25.236 kb on + strand, within GFF6235 at 25.236 kb on + strand, within GFF6235 at 25.237 kb on - strand, within GFF6235 at 25.237 kb on - strand, within GFF6235 at 25.328 kb on + strand, within GFF6235 at 25.328 kb on + strand, within GFF6235 at 25.329 kb on - strand, within GFF6235 at 25.335 kb on - strand, within GFF6235 at 25.335 kb on - strand, within GFF6235 at 25.475 kb on + strand, within GFF6235 at 25.475 kb on + strand, within GFF6235 at 25.511 kb on + strand at 25.850 kb on + strand at 25.883 kb on - strand at 25.883 kb on - strand at 25.889 kb on + strand at 25.895 kb on + strand at 26.025 kb on - strand at 26.035 kb on - strand at 26.344 kb on + strand at 26.344 kb on + strand at 26.345 kb on - strand at 26.345 kb on - strand at 26.345 kb on - strand at 26.345 kb on - strand at 26.488 kb on + strand, within GFF6236 at 26.489 kb on - strand, within GFF6236 at 26.489 kb on - strand, within GFF6236 at 26.503 kb on + strand, within GFF6236 at 26.503 kb on + strand, within GFF6236 at 26.504 kb on - strand, within GFF6236 at 26.515 kb on + strand, within GFF6236 at 26.549 kb on + strand, within GFF6236 at 26.550 kb on - strand, within GFF6236 at 26.860 kb on + strand, within GFF6236 at 27.037 kb on + strand, within GFF6236 at 27.037 kb on + strand, within GFF6236 at 27.038 kb on - strand, within GFF6236 at 27.058 kb on + strand, within GFF6236 at 27.059 kb on - strand, within GFF6236 at 27.253 kb on + strand, within GFF6236 at 27.626 kb on + strand, within GFF6236 at 27.626 kb on + strand, within GFF6236 at 27.627 kb on - strand, within GFF6236 at 27.627 kb on - strand, within GFF6236 at 27.627 kb on - strand, within GFF6236 at 28.069 kb on - strand, within GFF6236 at 28.447 kb on + strand, within GFF6236 at 28.448 kb on - strand, within GFF6236 at 28.487 kb on - strand at 28.487 kb on - strand at 28.493 kb on - strand at 28.578 kb on - strand at 28.578 kb on - strand at 28.578 kb on - strand at 28.578 kb on - strand at 28.598 kb on - strand at 28.598 kb on - strand at 28.598 kb on - strand at 28.695 kb on - strand at 28.745 kb on - strand at 28.745 kb on - strand at 28.754 kb on - strand at 28.836 kb on + strand at 28.837 kb on - strand at 28.837 kb on - strand at 28.837 kb on - strand at 29.355 kb on + strand, within GFF6237 at 29.356 kb on - strand, within GFF6237 at 29.381 kb on - strand, within GFF6237 at 29.419 kb on - strand, within GFF6237 at 29.419 kb on - strand, within GFF6237 at 29.419 kb on - strand, within GFF6237 at 29.430 kb on + strand, within GFF6237 at 29.437 kb on - strand, within GFF6237 at 29.478 kb on + strand, within GFF6237 at 29.507 kb on + strand, within GFF6237 at 29.507 kb on + strand, within GFF6237 at 29.572 kb on - strand, within GFF6237 at 29.572 kb on - strand, within GFF6237
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 25,146 - GFF6235 0.74 -0.8 25,178 + GFF6235 0.75 +1.0 25,178 + GFF6235 0.75 -0.2 25,179 - GFF6235 0.75 +1.0 25,179 - GFF6235 0.75 +2.0 25,179 - GFF6235 0.75 -0.2 25,236 + GFF6235 0.78 -0.9 25,236 + GFF6235 0.78 -1.0 25,237 - GFF6235 0.78 -0.2 25,237 - GFF6235 0.78 -0.4 25,328 + GFF6235 0.82 -0.5 25,328 + GFF6235 0.82 +0.1 25,329 - GFF6235 0.82 -0.3 25,335 - GFF6235 0.83 -0.6 25,335 - GFF6235 0.83 -0.5 25,475 + GFF6235 0.89 +0.3 25,475 + GFF6235 0.89 -1.0 25,511 + +0.7 25,850 + -0.8 25,883 - +0.8 25,883 - -2.2 25,889 + -0.8 25,895 + +0.0 26,025 - +0.1 26,035 - -0.8 26,344 + -0.4 26,344 + -0.5 26,345 - -1.5 26,345 - -0.2 26,345 - -1.2 26,345 - -0.6 26,488 + GFF6236 0.15 +1.0 26,489 - GFF6236 0.15 -0.6 26,489 - GFF6236 0.15 -0.7 26,503 + GFF6236 0.15 -0.4 26,503 + GFF6236 0.15 -0.5 26,504 - GFF6236 0.16 -0.2 26,515 + GFF6236 0.16 +0.4 26,549 + GFF6236 0.17 +0.1 26,550 - GFF6236 0.17 -1.2 26,860 + GFF6236 0.29 +0.4 27,037 + GFF6236 0.36 +1.4 27,037 + GFF6236 0.36 -0.9 27,038 - GFF6236 0.36 +0.3 27,058 + GFF6236 0.36 -1.9 27,059 - GFF6236 0.36 -0.8 27,253 + GFF6236 0.44 +1.0 27,626 + GFF6236 0.58 -0.8 27,626 + GFF6236 0.58 -0.6 27,627 - GFF6236 0.58 -0.8 27,627 - GFF6236 0.58 -2.8 27,627 - GFF6236 0.58 -1.2 28,069 - GFF6236 0.74 -0.5 28,447 + GFF6236 0.89 -0.8 28,448 - GFF6236 0.89 -0.8 28,487 - -0.5 28,487 - -0.8 28,493 - +0.6 28,578 - +1.0 28,578 - -0.0 28,578 - -0.8 28,578 - -0.1 28,598 - +0.7 28,598 - +0.8 28,598 - +1.0 28,695 - -1.2 28,745 - -1.3 28,745 - -0.1 28,754 - +0.7 28,836 + -0.1 28,837 - +0.5 28,837 - +0.0 28,837 - +2.5 29,355 + GFF6237 0.57 -1.0 29,356 - GFF6237 0.57 -1.1 29,381 - GFF6237 0.59 +0.8 29,419 - GFF6237 0.63 -0.1 29,419 - GFF6237 0.63 +0.9 29,419 - GFF6237 0.63 +0.0 29,430 + GFF6237 0.64 +0.2 29,437 - GFF6237 0.65 -0.5 29,478 + GFF6237 0.69 -1.1 29,507 + GFF6237 0.71 -0.2 29,507 + GFF6237 0.71 -0.3 29,572 - GFF6237 0.77 -1.1 29,572 - GFF6237 0.77 +0.0
Or see this region's nucleotide sequence