Strain Fitness in Variovorax sp. SCN45 around GFF6060

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF6058 and GFF6059 are separated by 18 nucleotidesGFF6059 and GFF6060 overlap by 11 nucleotidesGFF6060 and GFF6061 overlap by 4 nucleotidesGFF6061 and GFF6062 are separated by 64 nucleotides GFF6058 - ABC transporter, permease protein 1 (cluster 1, maltose/g3p/polyamine/iron), at 119,902 to 120,732 GFF6058 GFF6059 - ABC transporter, permease protein 2 (cluster 1, maltose/g3p/polyamine/iron), at 120,751 to 121,554 GFF6059 GFF6060 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 121,544 to 122,608 GFF6060 GFF6061 - 3',5'-cyclic-nucleotide phosphodiesterase (EC 3.1.4.17), at 122,605 to 123,468 GFF6061 GFF6062 - Sodium-dependent phosphate transporter, at 123,533 to 125,194 GFF6062 Position (kb) 121 122 123Strain fitness (log2 ratio) -2 -1 0 1 2at 120.607 kb on + strand, within GFF6058at 120.608 kb on - strand, within GFF6058at 120.608 kb on - strand, within GFF6058at 120.625 kb on + strand, within GFF6058at 120.625 kb on + strand, within GFF6058at 120.625 kb on + strand, within GFF6058at 120.625 kb on + strand, within GFF6058at 120.625 kb on + strand, within GFF6058at 120.626 kb on - strand, within GFF6058at 120.626 kb on - strand, within GFF6058at 120.626 kb on - strand, within GFF6058at 120.857 kb on + strand, within GFF6059at 120.858 kb on - strand, within GFF6059at 120.858 kb on - strand, within GFF6059at 120.868 kb on + strand, within GFF6059at 120.868 kb on + strand, within GFF6059at 120.869 kb on - strand, within GFF6059at 120.928 kb on + strand, within GFF6059at 121.010 kb on - strand, within GFF6059at 121.010 kb on - strand, within GFF6059at 121.010 kb on - strand, within GFF6059at 121.333 kb on - strand, within GFF6059at 121.432 kb on + strand, within GFF6059at 121.432 kb on + strand, within GFF6059at 121.432 kb on + strand, within GFF6059at 121.432 kb on + strand, within GFF6059at 121.432 kb on + strand, within GFF6059at 121.432 kb on + strand, within GFF6059at 121.432 kb on + strand, within GFF6059at 121.432 kb on + strand, within GFF6059at 121.433 kb on - strand, within GFF6059at 121.433 kb on - strand, within GFF6059at 121.433 kb on - strand, within GFF6059at 121.433 kb on - strand, within GFF6059at 121.433 kb on - strand, within GFF6059at 121.433 kb on - strand, within GFF6059at 121.433 kb on - strand, within GFF6059at 121.465 kb on + strand, within GFF6059at 121.516 kb on + strandat 121.516 kb on + strandat 121.517 kb on - strandat 121.517 kb on - strandat 121.592 kb on + strandat 121.592 kb on + strandat 121.592 kb on + strandat 121.593 kb on - strandat 121.593 kb on - strandat 121.811 kb on + strand, within GFF6060at 121.811 kb on + strand, within GFF6060at 121.812 kb on - strand, within GFF6060at 121.812 kb on - strand, within GFF6060at 122.689 kb on + strandat 122.689 kb on + strandat 122.690 kb on - strandat 122.690 kb on - strandat 122.713 kb on + strand, within GFF6061at 122.812 kb on + strand, within GFF6061at 122.813 kb on - strand, within GFF6061at 122.857 kb on + strand, within GFF6061at 122.857 kb on + strand, within GFF6061at 122.857 kb on + strand, within GFF6061at 122.920 kb on + strand, within GFF6061at 122.920 kb on + strand, within GFF6061at 122.920 kb on + strand, within GFF6061at 122.921 kb on - strand, within GFF6061at 122.921 kb on - strand, within GFF6061at 122.921 kb on - strand, within GFF6061at 123.417 kb on + strandat 123.518 kb on + strandat 123.543 kb on + strandat 123.543 kb on + strandat 123.543 kb on + strandat 123.543 kb on + strandat 123.544 kb on - strandat 123.544 kb on - strandat 123.544 kb on - strandat 123.544 kb on - strandat 123.544 kb on - strandat 123.544 kb on - strandat 123.544 kb on - strandat 123.583 kb on + strandat 123.598 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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120,607 + GFF6058 0.85 +0.5
120,608 - GFF6058 0.85 +0.1
120,608 - GFF6058 0.85 -0.5
120,625 + GFF6058 0.87 -0.2
120,625 + GFF6058 0.87 -0.3
120,625 + GFF6058 0.87 +0.5
120,625 + GFF6058 0.87 -1.0
120,625 + GFF6058 0.87 +0.1
120,626 - GFF6058 0.87 -0.3
120,626 - GFF6058 0.87 +0.1
120,626 - GFF6058 0.87 -0.6
120,857 + GFF6059 0.13 -0.5
120,858 - GFF6059 0.13 +0.4
120,858 - GFF6059 0.13 -0.3
120,868 + GFF6059 0.15 -0.8
120,868 + GFF6059 0.15 +0.5
120,869 - GFF6059 0.15 +0.8
120,928 + GFF6059 0.22 -0.1
121,010 - GFF6059 0.32 -0.9
121,010 - GFF6059 0.32 -2.4
121,010 - GFF6059 0.32 +1.5
121,333 - GFF6059 0.72 +0.2
121,432 + GFF6059 0.85 -1.0
121,432 + GFF6059 0.85 +1.5
121,432 + GFF6059 0.85 +0.3
121,432 + GFF6059 0.85 -0.7
121,432 + GFF6059 0.85 -0.2
121,432 + GFF6059 0.85 -0.0
121,432 + GFF6059 0.85 -0.4
121,432 + GFF6059 0.85 -0.7
121,433 - GFF6059 0.85 -0.2
121,433 - GFF6059 0.85 -1.4
121,433 - GFF6059 0.85 +0.6
121,433 - GFF6059 0.85 +0.1
121,433 - GFF6059 0.85 -0.9
121,433 - GFF6059 0.85 +1.0
121,433 - GFF6059 0.85 +0.1
121,465 + GFF6059 0.89 -0.5
121,516 + +0.8
121,516 + +0.2
121,517 - -0.7
121,517 - -0.6
121,592 + -0.6
121,592 + +0.0
121,592 + +0.3
121,593 - +0.5
121,593 - -0.5
121,811 + GFF6060 0.25 +1.8
121,811 + GFF6060 0.25 -1.3
121,812 - GFF6060 0.25 -0.4
121,812 - GFF6060 0.25 -0.6
122,689 + +0.8
122,689 + -0.7
122,690 - +0.2
122,690 - +0.6
122,713 + GFF6061 0.12 -1.3
122,812 + GFF6061 0.24 -0.4
122,813 - GFF6061 0.24 -0.2
122,857 + GFF6061 0.29 +0.6
122,857 + GFF6061 0.29 +0.6
122,857 + GFF6061 0.29 -0.4
122,920 + GFF6061 0.36 +0.1
122,920 + GFF6061 0.36 -1.3
122,920 + GFF6061 0.36 -0.4
122,921 - GFF6061 0.37 -0.5
122,921 - GFF6061 0.37 -0.3
122,921 - GFF6061 0.37 -0.1
123,417 + -0.2
123,518 + -1.9
123,543 + -1.1
123,543 + -0.7
123,543 + +0.6
123,543 + -1.3
123,544 - +0.5
123,544 - +0.7
123,544 - -1.2
123,544 - +0.9
123,544 - -0.1
123,544 - -0.2
123,544 - -0.0
123,583 + +1.2
123,598 + -1.4

Or see this region's nucleotide sequence