Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5644 and GFF5645 are separated by 24 nucleotides GFF5645 and GFF5646 overlap by 4 nucleotides GFF5646 and GFF5647 overlap by 4 nucleotides GFF5647 and GFF5648 are separated by 10 nucleotides
GFF5644 - Selenophosphate-dependent tRNA 2-selenouridine synthase, at 19,318 to 20,367
GFF5644
GFF5645 - ABC transporter, permease protein (cluster 12, methionine/phosphonates) / ABC transporter, permease protein (cluster 12, methionine/phosphonates), at 20,392 to 21,201
GFF5645
GFF5646 - ABC transporter, permease protein (cluster 12, methionine/phosphonates) / ABC transporter, permease protein (cluster 12, methionine/phosphonates), at 21,198 to 22,037
GFF5646
GFF5647 - ABC transporter, ATP-binding protein (cluster 12, methionine/phosphonates), at 22,034 to 22,846
GFF5647
GFF5648 - ABC transporter, substrate-binding protein (cluster 12, methionine/phosphonates), at 22,857 to 23,717
GFF5648
Position (kb) 21
22
23 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 20.210 kb on + strand, within GFF5644 at 20.210 kb on + strand, within GFF5644 at 20.211 kb on - strand, within GFF5644 at 20.211 kb on - strand, within GFF5644 at 20.274 kb on + strand at 20.274 kb on + strand at 20.274 kb on + strand at 20.275 kb on - strand at 20.275 kb on - strand at 20.399 kb on + strand at 20.399 kb on + strand at 20.399 kb on + strand at 20.399 kb on + strand at 20.400 kb on - strand at 20.400 kb on - strand at 20.400 kb on - strand at 20.519 kb on + strand, within GFF5645 at 20.519 kb on + strand, within GFF5645 at 20.520 kb on - strand, within GFF5645 at 20.520 kb on - strand, within GFF5645 at 20.594 kb on + strand, within GFF5645 at 20.642 kb on + strand, within GFF5645 at 20.642 kb on + strand, within GFF5645 at 20.643 kb on - strand, within GFF5645 at 21.098 kb on + strand, within GFF5645 at 21.388 kb on + strand, within GFF5646 at 21.388 kb on + strand, within GFF5646 at 21.388 kb on + strand, within GFF5646 at 21.388 kb on + strand, within GFF5646 at 21.389 kb on - strand, within GFF5646 at 21.389 kb on - strand, within GFF5646 at 21.397 kb on + strand, within GFF5646 at 21.397 kb on + strand, within GFF5646 at 21.520 kb on + strand, within GFF5646 at 21.521 kb on - strand, within GFF5646 at 21.544 kb on + strand, within GFF5646 at 21.544 kb on + strand, within GFF5646 at 21.544 kb on + strand, within GFF5646 at 21.544 kb on + strand, within GFF5646 at 21.544 kb on + strand, within GFF5646 at 21.545 kb on - strand, within GFF5646 at 21.545 kb on - strand, within GFF5646 at 21.545 kb on - strand, within GFF5646 at 21.545 kb on - strand, within GFF5646 at 21.545 kb on - strand, within GFF5646 at 21.545 kb on - strand, within GFF5646 at 21.545 kb on - strand, within GFF5646 at 21.545 kb on - strand, within GFF5646 at 21.545 kb on - strand, within GFF5646 at 21.545 kb on - strand, within GFF5646 at 22.119 kb on + strand, within GFF5647 at 22.119 kb on + strand, within GFF5647 at 22.119 kb on + strand, within GFF5647 at 22.120 kb on - strand, within GFF5647 at 22.362 kb on + strand, within GFF5647 at 22.363 kb on - strand, within GFF5647 at 22.363 kb on - strand, within GFF5647 at 22.363 kb on - strand, within GFF5647 at 22.363 kb on - strand, within GFF5647 at 22.903 kb on + strand at 22.903 kb on + strand at 22.903 kb on + strand at 22.903 kb on + strand at 22.903 kb on + strand at 22.903 kb on + strand at 22.904 kb on - strand at 22.904 kb on - strand at 22.904 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 20,210 + GFF5644 0.85 +0.3 20,210 + GFF5644 0.85 -1.0 20,211 - GFF5644 0.85 -0.1 20,211 - GFF5644 0.85 -1.1 20,274 + -0.2 20,274 + -0.2 20,274 + -0.1 20,275 - +0.6 20,275 - -0.4 20,399 + -1.9 20,399 + +0.1 20,399 + -0.7 20,399 + -1.4 20,400 - +1.1 20,400 - -1.4 20,400 - -0.4 20,519 + GFF5645 0.16 -1.6 20,519 + GFF5645 0.16 +1.0 20,520 - GFF5645 0.16 +0.6 20,520 - GFF5645 0.16 +0.5 20,594 + GFF5645 0.25 -0.1 20,642 + GFF5645 0.31 -0.5 20,642 + GFF5645 0.31 -0.5 20,643 - GFF5645 0.31 -1.1 21,098 + GFF5645 0.87 -0.9 21,388 + GFF5646 0.23 +0.4 21,388 + GFF5646 0.23 -0.3 21,388 + GFF5646 0.23 -0.6 21,388 + GFF5646 0.23 +1.2 21,389 - GFF5646 0.23 -0.6 21,389 - GFF5646 0.23 +1.6 21,397 + GFF5646 0.24 -0.8 21,397 + GFF5646 0.24 -0.7 21,520 + GFF5646 0.38 +0.9 21,521 - GFF5646 0.38 +0.9 21,544 + GFF5646 0.41 +0.7 21,544 + GFF5646 0.41 +1.9 21,544 + GFF5646 0.41 -0.6 21,544 + GFF5646 0.41 -0.2 21,544 + GFF5646 0.41 +0.6 21,545 - GFF5646 0.41 -0.4 21,545 - GFF5646 0.41 +0.1 21,545 - GFF5646 0.41 +1.1 21,545 - GFF5646 0.41 +0.1 21,545 - GFF5646 0.41 +0.3 21,545 - GFF5646 0.41 +0.9 21,545 - GFF5646 0.41 +0.3 21,545 - GFF5646 0.41 -2.0 21,545 - GFF5646 0.41 -2.9 21,545 - GFF5646 0.41 -0.1 22,119 + GFF5647 0.10 -0.1 22,119 + GFF5647 0.10 -0.9 22,119 + GFF5647 0.10 -0.2 22,120 - GFF5647 0.11 -0.8 22,362 + GFF5647 0.40 -1.2 22,363 - GFF5647 0.40 +0.1 22,363 - GFF5647 0.40 -2.2 22,363 - GFF5647 0.40 -0.2 22,363 - GFF5647 0.40 +0.0 22,903 + -1.2 22,903 + +0.3 22,903 + -1.1 22,903 + +1.3 22,903 + -0.4 22,903 + -1.8 22,904 - +0.4 22,904 - -0.5 22,904 - +1.6
Or see this region's nucleotide sequence