Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5615 and GFF5616 are separated by 90 nucleotides GFF5616 and GFF5617 overlap by 10 nucleotides
GFF5615 - Citrate synthase (si) (EC 2.3.3.1), at 237,620 to 238,759
GFF5615
GFF5616 - hypothetical protein, at 238,850 to 239,887
GFF5616
GFF5617 - Coenzyme A ligase, at 239,878 to 241,128
GFF5617
Position (kb) 238
239
240 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 238.005 kb on - strand, within GFF5615 at 238.142 kb on + strand, within GFF5615 at 238.395 kb on - strand, within GFF5615 at 238.568 kb on + strand, within GFF5615 at 238.569 kb on - strand, within GFF5615 at 238.709 kb on + strand at 238.710 kb on - strand at 238.710 kb on - strand at 238.763 kb on + strand at 238.763 kb on + strand at 238.764 kb on - strand at 238.764 kb on - strand at 238.841 kb on + strand at 238.842 kb on - strand at 238.842 kb on - strand at 238.842 kb on - strand at 239.012 kb on + strand, within GFF5616 at 239.013 kb on - strand, within GFF5616 at 239.013 kb on - strand, within GFF5616 at 239.174 kb on + strand, within GFF5616 at 239.201 kb on + strand, within GFF5616 at 239.201 kb on + strand, within GFF5616 at 239.202 kb on - strand, within GFF5616 at 239.202 kb on - strand, within GFF5616 at 239.223 kb on - strand, within GFF5616 at 239.744 kb on + strand, within GFF5616 at 239.744 kb on + strand, within GFF5616 at 239.744 kb on + strand, within GFF5616 at 239.744 kb on + strand, within GFF5616 at 239.745 kb on - strand, within GFF5616 at 239.745 kb on - strand, within GFF5616 at 239.870 kb on + strand at 239.871 kb on - strand at 239.879 kb on + strand at 239.885 kb on + strand at 240.404 kb on + strand, within GFF5617 at 240.404 kb on + strand, within GFF5617 at 240.434 kb on + strand, within GFF5617 at 240.435 kb on - strand, within GFF5617 at 240.435 kb on - strand, within GFF5617 at 240.565 kb on + strand, within GFF5617 at 240.565 kb on + strand, within GFF5617 at 240.565 kb on + strand, within GFF5617 at 240.565 kb on + strand, within GFF5617 at 240.566 kb on - strand, within GFF5617 at 240.723 kb on - strand, within GFF5617 at 240.821 kb on + strand, within GFF5617 at 240.822 kb on - strand, within GFF5617
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 238,005 - GFF5615 0.34 +1.0 238,142 + GFF5615 0.46 +0.4 238,395 - GFF5615 0.68 +0.1 238,568 + GFF5615 0.83 -1.1 238,569 - GFF5615 0.83 -0.1 238,709 + +0.8 238,710 - -0.7 238,710 - -0.4 238,763 + -1.9 238,763 + -0.6 238,764 - +0.1 238,764 - -0.2 238,841 + +1.3 238,842 - +1.3 238,842 - +0.7 238,842 - +0.4 239,012 + GFF5616 0.16 +0.2 239,013 - GFF5616 0.16 +0.1 239,013 - GFF5616 0.16 -0.1 239,174 + GFF5616 0.31 -0.1 239,201 + GFF5616 0.34 -0.3 239,201 + GFF5616 0.34 -0.1 239,202 - GFF5616 0.34 -0.7 239,202 - GFF5616 0.34 +0.0 239,223 - GFF5616 0.36 +1.5 239,744 + GFF5616 0.86 +1.3 239,744 + GFF5616 0.86 -2.2 239,744 + GFF5616 0.86 +0.1 239,744 + GFF5616 0.86 -2.6 239,745 - GFF5616 0.86 -0.7 239,745 - GFF5616 0.86 +0.7 239,870 + -1.0 239,871 - +2.1 239,879 + -0.7 239,885 + -2.9 240,404 + GFF5617 0.42 +0.1 240,404 + GFF5617 0.42 -1.4 240,434 + GFF5617 0.44 -0.7 240,435 - GFF5617 0.45 +1.5 240,435 - GFF5617 0.45 -0.5 240,565 + GFF5617 0.55 +0.4 240,565 + GFF5617 0.55 -1.7 240,565 + GFF5617 0.55 -0.2 240,565 + GFF5617 0.55 -0.5 240,566 - GFF5617 0.55 -1.5 240,723 - GFF5617 0.68 -0.9 240,821 + GFF5617 0.75 +0.3 240,822 - GFF5617 0.75 -1.7
Or see this region's nucleotide sequence