Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5460 and GFF5461 are separated by 12 nucleotides GFF5461 and GFF5462 overlap by 4 nucleotides GFF5462 and GFF5463 overlap by 4 nucleotides GFF5463 and GFF5464 overlap by 8 nucleotides
GFF5460 - 3-oxo-tetronate 4-phosphate decarboxylase, at 91,087 to 91,752
GFF5460
GFF5461 - 2-dehydrotetronate isomerase (EC 5.3.1.35), at 91,765 to 92,583
GFF5461
GFF5462 - Cytosine deaminase (EC 3.5.4.1), at 92,580 to 93,776
GFF5462
GFF5463 - RidA/YER057c/UK114 superfamily protein, at 93,773 to 94,240
GFF5463
GFF5464 - Transcriptional regulator, AcrR family, at 94,233 to 94,919
GFF5464
Position (kb)
92
93
94 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 91.753 kb on + strand at 91.753 kb on + strand at 91.754 kb on - strand at 91.754 kb on - strand at 91.864 kb on + strand, within GFF5461 at 91.864 kb on + strand, within GFF5461 at 91.864 kb on + strand, within GFF5461 at 91.864 kb on + strand, within GFF5461 at 91.865 kb on - strand, within GFF5461 at 91.865 kb on - strand, within GFF5461 at 91.865 kb on - strand, within GFF5461 at 91.876 kb on + strand, within GFF5461 at 92.128 kb on + strand, within GFF5461 at 92.128 kb on + strand, within GFF5461 at 92.128 kb on + strand, within GFF5461 at 92.128 kb on + strand, within GFF5461 at 92.129 kb on - strand, within GFF5461 at 92.129 kb on - strand, within GFF5461 at 92.129 kb on - strand, within GFF5461 at 92.183 kb on + strand, within GFF5461 at 92.184 kb on - strand, within GFF5461 at 92.184 kb on - strand, within GFF5461 at 92.302 kb on + strand, within GFF5461 at 92.302 kb on + strand, within GFF5461 at 92.302 kb on + strand, within GFF5461 at 92.303 kb on - strand, within GFF5461 at 92.303 kb on - strand, within GFF5461 at 92.303 kb on - strand, within GFF5461 at 92.339 kb on - strand, within GFF5461 at 92.350 kb on + strand, within GFF5461 at 92.350 kb on + strand, within GFF5461 at 92.351 kb on - strand, within GFF5461 at 92.351 kb on - strand, within GFF5461 at 92.431 kb on + strand, within GFF5461 at 92.431 kb on + strand, within GFF5461 at 92.431 kb on + strand, within GFF5461 at 92.431 kb on + strand, within GFF5461 at 92.431 kb on + strand, within GFF5461 at 92.431 kb on + strand, within GFF5461 at 92.431 kb on + strand, within GFF5461 at 92.431 kb on + strand, within GFF5461 at 92.432 kb on - strand, within GFF5461 at 92.432 kb on - strand, within GFF5461 at 92.432 kb on - strand, within GFF5461 at 92.432 kb on - strand, within GFF5461 at 92.432 kb on - strand, within GFF5461 at 92.432 kb on - strand, within GFF5461 at 92.432 kb on - strand, within GFF5461 at 92.437 kb on + strand, within GFF5461 at 92.437 kb on + strand, within GFF5461 at 92.437 kb on + strand, within GFF5461 at 92.437 kb on + strand, within GFF5461 at 92.437 kb on + strand, within GFF5461 at 92.437 kb on + strand, within GFF5461 at 92.438 kb on - strand, within GFF5461 at 92.438 kb on - strand, within GFF5461 at 92.503 kb on + strand at 92.503 kb on + strand at 92.737 kb on + strand, within GFF5462 at 92.737 kb on + strand, within GFF5462 at 92.737 kb on + strand, within GFF5462 at 92.738 kb on - strand, within GFF5462 at 92.766 kb on + strand, within GFF5462 at 92.766 kb on + strand, within GFF5462 at 92.766 kb on + strand, within GFF5462 at 92.766 kb on + strand, within GFF5462 at 92.766 kb on + strand, within GFF5462 at 92.766 kb on + strand, within GFF5462 at 92.766 kb on + strand, within GFF5462 at 92.766 kb on + strand, within GFF5462 at 92.766 kb on + strand, within GFF5462 at 92.767 kb on - strand, within GFF5462 at 92.767 kb on - strand, within GFF5462 at 92.767 kb on - strand, within GFF5462 at 92.767 kb on - strand, within GFF5462 at 93.118 kb on - strand, within GFF5462 at 93.397 kb on - strand, within GFF5462 at 93.397 kb on - strand, within GFF5462 at 93.397 kb on - strand, within GFF5462 at 93.513 kb on + strand, within GFF5462 at 93.513 kb on + strand, within GFF5462 at 93.513 kb on + strand, within GFF5462 at 93.514 kb on - strand, within GFF5462 at 93.514 kb on - strand, within GFF5462 at 93.514 kb on - strand, within GFF5462 at 93.514 kb on - strand, within GFF5462 at 93.514 kb on - strand, within GFF5462 at 93.514 kb on - strand, within GFF5462 at 93.514 kb on - strand, within GFF5462 at 93.514 kb on - strand, within GFF5462 at 93.514 kb on - strand, within GFF5462 at 93.514 kb on - strand, within GFF5462 at 93.519 kb on + strand, within GFF5462 at 93.528 kb on + strand, within GFF5462 at 93.528 kb on + strand, within GFF5462 at 93.528 kb on + strand, within GFF5462 at 93.528 kb on + strand, within GFF5462 at 93.528 kb on + strand, within GFF5462 at 93.528 kb on + strand, within GFF5462 at 93.528 kb on + strand, within GFF5462 at 93.528 kb on + strand, within GFF5462 at 93.528 kb on + strand, within GFF5462 at 93.529 kb on - strand, within GFF5462 at 93.682 kb on + strand at 93.682 kb on + strand at 93.683 kb on - strand at 93.683 kb on - strand at 93.683 kb on - strand at 93.800 kb on + strand at 93.800 kb on + strand at 93.800 kb on + strand at 93.800 kb on + strand at 93.800 kb on + strand at 93.801 kb on - strand at 93.801 kb on - strand at 93.801 kb on - strand at 93.801 kb on - strand at 93.801 kb on - strand at 93.888 kb on - strand, within GFF5463 at 94.070 kb on + strand, within GFF5463 at 94.071 kb on - strand, within GFF5463 at 94.071 kb on - strand, within GFF5463 at 94.071 kb on - strand, within GFF5463 at 94.071 kb on - strand, within GFF5463 at 94.071 kb on - strand, within GFF5463 at 94.092 kb on - strand, within GFF5463 at 94.092 kb on - strand, within GFF5463 at 94.100 kb on + strand, within GFF5463 at 94.100 kb on + strand, within GFF5463 at 94.100 kb on + strand, within GFF5463 at 94.100 kb on + strand, within GFF5463 at 94.100 kb on + strand, within GFF5463 at 94.100 kb on + strand, within GFF5463 at 94.100 kb on + strand, within GFF5463 at 94.101 kb on - strand, within GFF5463 at 94.101 kb on - strand, within GFF5463 at 94.101 kb on - strand, within GFF5463 at 94.101 kb on - strand, within GFF5463 at 94.101 kb on - strand, within GFF5463 at 94.101 kb on - strand, within GFF5463 at 94.101 kb on - strand, within GFF5463 at 94.125 kb on - strand, within GFF5463 at 94.214 kb on + strand at 94.214 kb on + strand at 94.214 kb on + strand at 94.214 kb on + strand at 94.214 kb on + strand at 94.214 kb on + strand at 94.214 kb on + strand at 94.214 kb on + strand at 94.214 kb on + strand at 94.214 kb on + strand at 94.214 kb on + strand at 94.214 kb on + strand at 94.215 kb on - strand at 94.215 kb on - strand at 94.215 kb on - strand at 94.215 kb on - strand at 94.215 kb on - strand at 94.215 kb on - strand at 94.215 kb on - strand at 94.215 kb on - strand at 94.215 kb on - strand at 94.215 kb on - strand at 94.215 kb on - strand at 94.215 kb on - strand at 94.234 kb on + strand at 94.234 kb on + strand at 94.235 kb on - strand at 94.235 kb on - strand at 94.235 kb on - strand at 94.285 kb on + strand at 94.286 kb on - strand at 94.328 kb on - strand, within GFF5464 at 94.328 kb on - strand, within GFF5464 at 94.330 kb on + strand, within GFF5464 at 94.330 kb on + strand, within GFF5464 at 94.357 kb on + strand, within GFF5464 at 94.357 kb on + strand, within GFF5464 at 94.358 kb on - strand, within GFF5464 at 94.358 kb on - strand, within GFF5464 at 94.443 kb on + strand, within GFF5464 at 94.444 kb on - strand, within GFF5464 at 94.699 kb on + strand, within GFF5464 at 94.699 kb on + strand, within GFF5464 at 94.699 kb on + strand, within GFF5464 at 94.699 kb on + strand, within GFF5464 at 94.699 kb on + strand, within GFF5464 at 94.699 kb on + strand, within GFF5464 at 94.699 kb on + strand, within GFF5464 at 94.700 kb on - strand, within GFF5464 at 94.700 kb on - strand, within GFF5464 at 94.700 kb on - strand, within GFF5464 at 94.700 kb on - strand, within GFF5464 at 94.702 kb on + strand, within GFF5464 at 94.702 kb on + strand, within GFF5464 at 94.702 kb on + strand, within GFF5464 at 94.702 kb on + strand, within GFF5464 at 94.702 kb on + strand, within GFF5464 at 94.703 kb on - strand, within GFF5464 at 94.703 kb on - strand, within GFF5464 at 94.703 kb on - strand, within GFF5464
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 91,753 + -0.1 91,753 + -0.0 91,754 - -0.4 91,754 - -0.6 91,864 + GFF5461 0.12 +1.2 91,864 + GFF5461 0.12 -0.3 91,864 + GFF5461 0.12 +0.3 91,864 + GFF5461 0.12 -1.7 91,865 - GFF5461 0.12 -0.2 91,865 - GFF5461 0.12 -0.7 91,865 - GFF5461 0.12 -0.1 91,876 + GFF5461 0.14 +0.5 92,128 + GFF5461 0.44 +1.3 92,128 + GFF5461 0.44 -0.2 92,128 + GFF5461 0.44 -0.4 92,128 + GFF5461 0.44 -0.7 92,129 - GFF5461 0.44 -0.8 92,129 - GFF5461 0.44 -1.7 92,129 - GFF5461 0.44 +0.5 92,183 + GFF5461 0.51 -0.2 92,184 - GFF5461 0.51 +0.2 92,184 - GFF5461 0.51 -0.8 92,302 + GFF5461 0.66 +0.0 92,302 + GFF5461 0.66 +0.0 92,302 + GFF5461 0.66 +1.1 92,303 - GFF5461 0.66 +1.7 92,303 - GFF5461 0.66 -0.9 92,303 - GFF5461 0.66 +0.1 92,339 - GFF5461 0.70 -1.1 92,350 + GFF5461 0.71 -0.9 92,350 + GFF5461 0.71 -2.0 92,351 - GFF5461 0.72 -0.2 92,351 - GFF5461 0.72 +1.5 92,431 + GFF5461 0.81 +1.1 92,431 + GFF5461 0.81 -1.4 92,431 + GFF5461 0.81 -1.7 92,431 + GFF5461 0.81 +0.2 92,431 + GFF5461 0.81 -0.4 92,431 + GFF5461 0.81 +1.1 92,431 + GFF5461 0.81 -0.3 92,431 + GFF5461 0.81 -0.6 92,432 - GFF5461 0.81 +1.6 92,432 - GFF5461 0.81 +0.0 92,432 - GFF5461 0.81 -1.0 92,432 - GFF5461 0.81 +0.1 92,432 - GFF5461 0.81 -0.5 92,432 - GFF5461 0.81 -2.5 92,432 - GFF5461 0.81 +0.9 92,437 + GFF5461 0.82 +1.8 92,437 + GFF5461 0.82 -0.6 92,437 + GFF5461 0.82 +2.1 92,437 + GFF5461 0.82 +0.6 92,437 + GFF5461 0.82 +0.9 92,437 + GFF5461 0.82 -0.6 92,438 - GFF5461 0.82 +1.1 92,438 - GFF5461 0.82 -1.1 92,503 + -1.2 92,503 + +0.1 92,737 + GFF5462 0.13 +0.6 92,737 + GFF5462 0.13 +0.9 92,737 + GFF5462 0.13 -1.6 92,738 - GFF5462 0.13 -1.0 92,766 + GFF5462 0.16 -0.1 92,766 + GFF5462 0.16 -2.4 92,766 + GFF5462 0.16 -1.1 92,766 + GFF5462 0.16 -0.5 92,766 + GFF5462 0.16 -0.7 92,766 + GFF5462 0.16 -1.6 92,766 + GFF5462 0.16 -1.7 92,766 + GFF5462 0.16 -0.9 92,766 + GFF5462 0.16 +0.3 92,767 - GFF5462 0.16 -2.7 92,767 - GFF5462 0.16 +0.4 92,767 - GFF5462 0.16 +1.1 92,767 - GFF5462 0.16 -0.3 93,118 - GFF5462 0.45 -0.9 93,397 - GFF5462 0.68 -2.8 93,397 - GFF5462 0.68 -1.2 93,397 - GFF5462 0.68 -0.7 93,513 + GFF5462 0.78 -1.1 93,513 + GFF5462 0.78 -1.2 93,513 + GFF5462 0.78 +0.1 93,514 - GFF5462 0.78 -0.7 93,514 - GFF5462 0.78 -0.7 93,514 - GFF5462 0.78 +0.9 93,514 - GFF5462 0.78 +0.2 93,514 - GFF5462 0.78 -0.9 93,514 - GFF5462 0.78 -2.0 93,514 - GFF5462 0.78 -0.6 93,514 - GFF5462 0.78 -2.1 93,514 - GFF5462 0.78 +0.5 93,514 - GFF5462 0.78 -0.3 93,519 + GFF5462 0.78 +0.6 93,528 + GFF5462 0.79 +1.3 93,528 + GFF5462 0.79 -0.5 93,528 + GFF5462 0.79 -0.7 93,528 + GFF5462 0.79 +0.1 93,528 + GFF5462 0.79 -0.3 93,528 + GFF5462 0.79 +0.5 93,528 + GFF5462 0.79 -0.2 93,528 + GFF5462 0.79 +0.2 93,528 + GFF5462 0.79 -0.5 93,529 - GFF5462 0.79 -2.2 93,682 + +0.5 93,682 + +0.3 93,683 - -0.7 93,683 - +0.7 93,683 - -0.4 93,800 + +0.6 93,800 + +0.1 93,800 + -0.1 93,800 + -0.6 93,800 + +0.5 93,801 - -1.7 93,801 - -2.2 93,801 - -0.2 93,801 - -0.9 93,801 - -0.5 93,888 - GFF5463 0.25 +1.1 94,070 + GFF5463 0.63 +2.1 94,071 - GFF5463 0.64 -0.2 94,071 - GFF5463 0.64 -0.4 94,071 - GFF5463 0.64 -0.6 94,071 - GFF5463 0.64 -0.4 94,071 - GFF5463 0.64 -0.8 94,092 - GFF5463 0.68 -0.6 94,092 - GFF5463 0.68 -0.2 94,100 + GFF5463 0.70 -0.5 94,100 + GFF5463 0.70 +2.1 94,100 + GFF5463 0.70 +0.9 94,100 + GFF5463 0.70 +0.1 94,100 + GFF5463 0.70 +0.5 94,100 + GFF5463 0.70 +0.6 94,100 + GFF5463 0.70 -0.3 94,101 - GFF5463 0.70 +0.1 94,101 - GFF5463 0.70 +0.3 94,101 - GFF5463 0.70 -0.1 94,101 - GFF5463 0.70 +0.2 94,101 - GFF5463 0.70 -1.2 94,101 - GFF5463 0.70 -0.4 94,101 - GFF5463 0.70 -1.4 94,125 - GFF5463 0.75 +1.1 94,214 + -0.5 94,214 + +0.1 94,214 + -1.8 94,214 + +1.2 94,214 + +0.5 94,214 + +1.1 94,214 + -0.1 94,214 + -1.2 94,214 + +1.7 94,214 + -0.5 94,214 + -0.6 94,214 + +1.1 94,215 - +0.2 94,215 - -0.4 94,215 - -1.7 94,215 - -0.6 94,215 - +0.1 94,215 - +0.2 94,215 - -0.5 94,215 - +0.5 94,215 - -0.6 94,215 - +0.2 94,215 - +0.8 94,215 - -0.7 94,234 + -0.5 94,234 + -0.2 94,235 - -0.1 94,235 - -0.4 94,235 - +0.0 94,285 + +0.5 94,286 - +0.1 94,328 - GFF5464 0.14 +0.7 94,328 - GFF5464 0.14 +0.8 94,330 + GFF5464 0.14 +0.4 94,330 + GFF5464 0.14 -0.9 94,357 + GFF5464 0.18 -1.7 94,357 + GFF5464 0.18 +0.4 94,358 - GFF5464 0.18 +0.5 94,358 - GFF5464 0.18 +0.1 94,443 + GFF5464 0.31 -1.6 94,444 - GFF5464 0.31 +0.2 94,699 + GFF5464 0.68 +0.1 94,699 + GFF5464 0.68 +0.5 94,699 + GFF5464 0.68 +0.2 94,699 + GFF5464 0.68 +1.7 94,699 + GFF5464 0.68 +0.4 94,699 + GFF5464 0.68 +0.3 94,699 + GFF5464 0.68 +0.6 94,700 - GFF5464 0.68 +3.1 94,700 - GFF5464 0.68 +0.1 94,700 - GFF5464 0.68 +0.8 94,700 - GFF5464 0.68 +0.5 94,702 + GFF5464 0.68 -0.2 94,702 + GFF5464 0.68 +0.9 94,702 + GFF5464 0.68 +0.8 94,702 + GFF5464 0.68 +1.0 94,702 + GFF5464 0.68 +1.5 94,703 - GFF5464 0.68 +0.0 94,703 - GFF5464 0.68 +0.2 94,703 - GFF5464 0.68 +0.5
Or see this region's nucleotide sequence