Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5393 and GFF5394 are separated by 5 nucleotides GFF5394 and GFF5395 are separated by 32 nucleotides GFF5395 and GFF5396 overlap by 17 nucleotides
GFF5393 - mandelate racemase/muconate lactonizing enzyme family protein, at 18,606 to 19,715
GFF5393
GFF5394 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 19,721 to 20,548
GFF5394
GFF5395 - Glutamine ABC transporter, ATP-binding protein GlnQ, at 20,581 to 21,309
GFF5395
GFF5396 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 21,293 to 21,949
GFF5396
Position (kb) 19
20
21 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 18.757 kb on + strand, within GFF5393 at 18.757 kb on + strand, within GFF5393 at 18.757 kb on + strand, within GFF5393 at 18.757 kb on + strand, within GFF5393 at 18.757 kb on + strand, within GFF5393 at 18.757 kb on + strand, within GFF5393 at 18.757 kb on + strand, within GFF5393 at 18.757 kb on + strand, within GFF5393 at 18.757 kb on + strand, within GFF5393 at 18.757 kb on + strand, within GFF5393 at 18.757 kb on + strand, within GFF5393 at 18.757 kb on + strand, within GFF5393 at 18.757 kb on + strand, within GFF5393 at 18.757 kb on + strand, within GFF5393 at 18.758 kb on - strand, within GFF5393 at 18.758 kb on - strand, within GFF5393 at 18.758 kb on - strand, within GFF5393 at 18.758 kb on - strand, within GFF5393 at 18.758 kb on - strand, within GFF5393 at 18.758 kb on - strand, within GFF5393 at 18.758 kb on - strand, within GFF5393 at 18.758 kb on - strand, within GFF5393 at 18.758 kb on - strand, within GFF5393 at 18.758 kb on - strand, within GFF5393 at 18.758 kb on - strand, within GFF5393 at 18.758 kb on - strand, within GFF5393 at 18.758 kb on - strand, within GFF5393 at 18.758 kb on - strand, within GFF5393 at 18.758 kb on - strand, within GFF5393 at 18.758 kb on - strand, within GFF5393 at 18.758 kb on - strand, within GFF5393 at 18.758 kb on - strand, within GFF5393 at 18.758 kb on - strand, within GFF5393 at 18.758 kb on - strand, within GFF5393 at 18.758 kb on - strand, within GFF5393 at 18.937 kb on + strand, within GFF5393 at 18.937 kb on + strand, within GFF5393 at 18.937 kb on + strand, within GFF5393 at 18.937 kb on + strand, within GFF5393 at 18.937 kb on + strand, within GFF5393 at 18.937 kb on + strand, within GFF5393 at 18.937 kb on + strand, within GFF5393 at 18.937 kb on + strand, within GFF5393 at 18.937 kb on + strand, within GFF5393 at 18.938 kb on - strand, within GFF5393 at 18.938 kb on - strand, within GFF5393 at 18.938 kb on - strand, within GFF5393 at 18.938 kb on - strand, within GFF5393 at 18.938 kb on - strand, within GFF5393 at 18.938 kb on - strand, within GFF5393 at 18.938 kb on - strand, within GFF5393 at 18.961 kb on + strand, within GFF5393 at 18.961 kb on + strand, within GFF5393 at 18.961 kb on + strand, within GFF5393 at 18.961 kb on + strand, within GFF5393 at 18.961 kb on + strand, within GFF5393 at 18.962 kb on - strand, within GFF5393 at 18.962 kb on - strand, within GFF5393 at 18.962 kb on - strand, within GFF5393 at 18.962 kb on - strand, within GFF5393 at 18.962 kb on - strand, within GFF5393 at 18.962 kb on - strand, within GFF5393 at 18.962 kb on - strand, within GFF5393 at 18.962 kb on - strand, within GFF5393 at 18.962 kb on - strand, within GFF5393 at 19.075 kb on + strand, within GFF5393 at 19.075 kb on + strand, within GFF5393 at 19.076 kb on - strand, within GFF5393 at 19.076 kb on - strand, within GFF5393 at 19.076 kb on - strand, within GFF5393 at 19.309 kb on + strand, within GFF5393 at 19.309 kb on + strand, within GFF5393 at 19.309 kb on + strand, within GFF5393 at 19.309 kb on + strand, within GFF5393 at 19.309 kb on + strand, within GFF5393 at 19.309 kb on + strand, within GFF5393 at 19.309 kb on + strand, within GFF5393 at 19.309 kb on + strand, within GFF5393 at 19.309 kb on + strand, within GFF5393 at 19.310 kb on - strand, within GFF5393 at 19.310 kb on - strand, within GFF5393 at 19.310 kb on - strand, within GFF5393 at 19.310 kb on - strand, within GFF5393 at 19.480 kb on + strand, within GFF5393 at 19.480 kb on + strand, within GFF5393 at 19.481 kb on - strand, within GFF5393 at 19.567 kb on + strand, within GFF5393 at 19.567 kb on + strand, within GFF5393 at 19.567 kb on + strand, within GFF5393 at 19.567 kb on + strand, within GFF5393 at 19.567 kb on + strand, within GFF5393 at 19.567 kb on + strand, within GFF5393 at 19.567 kb on + strand, within GFF5393 at 19.567 kb on + strand, within GFF5393 at 19.567 kb on + strand, within GFF5393 at 19.567 kb on + strand, within GFF5393 at 19.567 kb on + strand, within GFF5393 at 19.567 kb on + strand, within GFF5393 at 19.567 kb on + strand, within GFF5393 at 19.567 kb on + strand, within GFF5393 at 19.567 kb on + strand, within GFF5393 at 19.567 kb on + strand, within GFF5393 at 19.567 kb on + strand, within GFF5393 at 19.568 kb on + strand, within GFF5393 at 19.568 kb on - strand, within GFF5393 at 19.568 kb on - strand, within GFF5393 at 19.568 kb on - strand, within GFF5393 at 19.568 kb on - strand, within GFF5393 at 19.568 kb on - strand, within GFF5393 at 19.568 kb on - strand, within GFF5393 at 19.568 kb on - strand, within GFF5393 at 19.568 kb on - strand, within GFF5393 at 19.568 kb on - strand, within GFF5393 at 19.568 kb on - strand, within GFF5393 at 19.568 kb on - strand, within GFF5393 at 19.568 kb on - strand, within GFF5393 at 19.568 kb on - strand, within GFF5393 at 19.568 kb on - strand, within GFF5393 at 19.568 kb on - strand, within GFF5393 at 19.568 kb on - strand, within GFF5393 at 19.568 kb on - strand, within GFF5393 at 19.573 kb on + strand, within GFF5393 at 19.573 kb on + strand, within GFF5393 at 19.573 kb on + strand, within GFF5393 at 19.574 kb on - strand, within GFF5393 at 19.776 kb on + strand at 19.776 kb on + strand at 19.776 kb on + strand at 19.776 kb on + strand at 19.776 kb on + strand at 19.776 kb on + strand at 19.776 kb on + strand at 19.776 kb on + strand at 19.776 kb on + strand at 19.776 kb on + strand at 19.777 kb on - strand at 19.777 kb on - strand at 19.777 kb on - strand at 19.777 kb on - strand at 19.777 kb on - strand at 19.777 kb on - strand at 19.777 kb on - strand at 20.169 kb on + strand, within GFF5394 at 20.169 kb on + strand, within GFF5394 at 20.169 kb on + strand, within GFF5394 at 20.169 kb on + strand, within GFF5394 at 20.380 kb on - strand, within GFF5394 at 20.380 kb on - strand, within GFF5394 at 20.380 kb on - strand, within GFF5394 at 20.678 kb on + strand, within GFF5395 at 20.678 kb on + strand, within GFF5395 at 20.678 kb on + strand, within GFF5395 at 20.678 kb on + strand, within GFF5395 at 20.678 kb on + strand, within GFF5395 at 20.678 kb on + strand, within GFF5395 at 20.678 kb on + strand, within GFF5395 at 20.678 kb on + strand, within GFF5395 at 20.678 kb on + strand, within GFF5395 at 20.678 kb on + strand, within GFF5395 at 20.678 kb on + strand, within GFF5395 at 20.678 kb on + strand, within GFF5395 at 20.678 kb on + strand, within GFF5395 at 20.678 kb on + strand, within GFF5395 at 20.678 kb on + strand, within GFF5395 at 20.679 kb on - strand, within GFF5395 at 20.679 kb on - strand, within GFF5395 at 20.679 kb on - strand, within GFF5395 at 20.679 kb on - strand, within GFF5395 at 20.679 kb on - strand, within GFF5395 at 20.679 kb on - strand, within GFF5395 at 20.679 kb on - strand, within GFF5395 at 20.679 kb on - strand, within GFF5395 at 20.679 kb on - strand, within GFF5395 at 20.679 kb on - strand, within GFF5395 at 20.679 kb on - strand, within GFF5395 at 20.679 kb on - strand, within GFF5395 at 20.679 kb on - strand, within GFF5395 at 20.679 kb on - strand, within GFF5395 at 21.044 kb on + strand, within GFF5395 at 21.044 kb on + strand, within GFF5395 at 21.044 kb on + strand, within GFF5395 at 21.044 kb on + strand, within GFF5395 at 21.044 kb on + strand, within GFF5395 at 21.044 kb on + strand, within GFF5395 at 21.044 kb on + strand, within GFF5395 at 21.044 kb on + strand, within GFF5395 at 21.045 kb on - strand, within GFF5395 at 21.045 kb on - strand, within GFF5395 at 21.045 kb on - strand, within GFF5395 at 21.045 kb on - strand, within GFF5395 at 21.045 kb on - strand, within GFF5395 at 21.045 kb on - strand, within GFF5395 at 21.045 kb on - strand, within GFF5395 at 21.045 kb on - strand, within GFF5395 at 21.366 kb on + strand, within GFF5396 at 21.366 kb on + strand, within GFF5396 at 21.366 kb on + strand, within GFF5396 at 21.367 kb on - strand, within GFF5396 at 21.367 kb on - strand, within GFF5396 at 21.367 kb on - strand, within GFF5396 at 21.390 kb on + strand, within GFF5396 at 21.390 kb on + strand, within GFF5396 at 21.435 kb on + strand, within GFF5396 at 21.435 kb on + strand, within GFF5396 at 21.435 kb on + strand, within GFF5396 at 21.435 kb on + strand, within GFF5396 at 21.435 kb on + strand, within GFF5396 at 21.435 kb on + strand, within GFF5396 at 21.435 kb on + strand, within GFF5396 at 21.436 kb on - strand, within GFF5396 at 21.436 kb on - strand, within GFF5396 at 21.436 kb on - strand, within GFF5396
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 18,757 + GFF5393 0.14 +0.1 18,757 + GFF5393 0.14 +1.3 18,757 + GFF5393 0.14 -0.2 18,757 + GFF5393 0.14 -1.3 18,757 + GFF5393 0.14 +0.5 18,757 + GFF5393 0.14 +0.2 18,757 + GFF5393 0.14 +1.1 18,757 + GFF5393 0.14 -0.5 18,757 + GFF5393 0.14 -0.7 18,757 + GFF5393 0.14 +0.0 18,757 + GFF5393 0.14 -0.4 18,757 + GFF5393 0.14 -1.7 18,757 + GFF5393 0.14 +0.8 18,757 + GFF5393 0.14 +1.2 18,758 - GFF5393 0.14 -1.1 18,758 - GFF5393 0.14 -1.5 18,758 - GFF5393 0.14 +0.1 18,758 - GFF5393 0.14 -1.1 18,758 - GFF5393 0.14 -1.6 18,758 - GFF5393 0.14 -0.5 18,758 - GFF5393 0.14 +0.9 18,758 - GFF5393 0.14 -2.5 18,758 - GFF5393 0.14 -0.4 18,758 - GFF5393 0.14 -0.6 18,758 - GFF5393 0.14 +0.9 18,758 - GFF5393 0.14 -0.6 18,758 - GFF5393 0.14 -1.4 18,758 - GFF5393 0.14 -0.0 18,758 - GFF5393 0.14 -0.2 18,758 - GFF5393 0.14 +0.6 18,758 - GFF5393 0.14 -1.9 18,758 - GFF5393 0.14 +0.4 18,758 - GFF5393 0.14 +1.6 18,758 - GFF5393 0.14 -0.6 18,758 - GFF5393 0.14 -0.9 18,937 + GFF5393 0.30 -0.9 18,937 + GFF5393 0.30 -0.2 18,937 + GFF5393 0.30 -0.1 18,937 + GFF5393 0.30 +0.5 18,937 + GFF5393 0.30 -1.0 18,937 + GFF5393 0.30 -0.8 18,937 + GFF5393 0.30 +0.6 18,937 + GFF5393 0.30 +0.1 18,937 + GFF5393 0.30 +0.3 18,938 - GFF5393 0.30 +0.6 18,938 - GFF5393 0.30 +0.3 18,938 - GFF5393 0.30 -0.9 18,938 - GFF5393 0.30 +0.1 18,938 - GFF5393 0.30 -0.8 18,938 - GFF5393 0.30 -0.6 18,938 - GFF5393 0.30 +0.4 18,961 + GFF5393 0.32 -3.0 18,961 + GFF5393 0.32 +1.7 18,961 + GFF5393 0.32 -0.6 18,961 + GFF5393 0.32 -0.0 18,961 + GFF5393 0.32 +1.5 18,962 - GFF5393 0.32 +1.1 18,962 - GFF5393 0.32 -0.2 18,962 - GFF5393 0.32 +0.7 18,962 - GFF5393 0.32 +0.1 18,962 - GFF5393 0.32 -0.5 18,962 - GFF5393 0.32 +0.1 18,962 - GFF5393 0.32 +0.6 18,962 - GFF5393 0.32 +1.1 18,962 - GFF5393 0.32 -0.8 19,075 + GFF5393 0.42 +0.8 19,075 + GFF5393 0.42 -0.7 19,076 - GFF5393 0.42 +0.2 19,076 - GFF5393 0.42 -0.2 19,076 - GFF5393 0.42 +0.2 19,309 + GFF5393 0.63 +1.2 19,309 + GFF5393 0.63 -0.4 19,309 + GFF5393 0.63 -1.5 19,309 + GFF5393 0.63 -0.5 19,309 + GFF5393 0.63 +0.0 19,309 + GFF5393 0.63 +0.2 19,309 + GFF5393 0.63 -0.0 19,309 + GFF5393 0.63 -0.6 19,309 + GFF5393 0.63 -0.7 19,310 - GFF5393 0.63 +0.2 19,310 - GFF5393 0.63 -0.5 19,310 - GFF5393 0.63 +0.5 19,310 - GFF5393 0.63 +1.3 19,480 + GFF5393 0.79 +1.1 19,480 + GFF5393 0.79 +0.1 19,481 - GFF5393 0.79 -1.1 19,567 + GFF5393 0.87 -0.6 19,567 + GFF5393 0.87 +0.1 19,567 + GFF5393 0.87 -0.1 19,567 + GFF5393 0.87 +0.6 19,567 + GFF5393 0.87 +0.8 19,567 + GFF5393 0.87 -0.5 19,567 + GFF5393 0.87 -0.6 19,567 + GFF5393 0.87 +0.6 19,567 + GFF5393 0.87 +0.0 19,567 + GFF5393 0.87 -0.6 19,567 + GFF5393 0.87 -0.7 19,567 + GFF5393 0.87 -0.8 19,567 + GFF5393 0.87 +0.7 19,567 + GFF5393 0.87 +0.7 19,567 + GFF5393 0.87 -0.1 19,567 + GFF5393 0.87 -1.1 19,567 + GFF5393 0.87 -2.2 19,568 + GFF5393 0.87 -0.8 19,568 - GFF5393 0.87 -0.3 19,568 - GFF5393 0.87 -1.2 19,568 - GFF5393 0.87 -0.6 19,568 - GFF5393 0.87 +0.3 19,568 - GFF5393 0.87 -0.3 19,568 - GFF5393 0.87 +1.5 19,568 - GFF5393 0.87 +0.6 19,568 - GFF5393 0.87 -0.1 19,568 - GFF5393 0.87 -0.5 19,568 - GFF5393 0.87 +0.3 19,568 - GFF5393 0.87 +0.9 19,568 - GFF5393 0.87 +1.1 19,568 - GFF5393 0.87 -0.2 19,568 - GFF5393 0.87 +0.6 19,568 - GFF5393 0.87 -0.8 19,568 - GFF5393 0.87 -0.8 19,568 - GFF5393 0.87 -2.6 19,573 + GFF5393 0.87 -0.4 19,573 + GFF5393 0.87 +0.2 19,573 + GFF5393 0.87 +0.2 19,574 - GFF5393 0.87 +1.1 19,776 + +0.5 19,776 + -0.2 19,776 + +0.1 19,776 + -0.0 19,776 + +0.3 19,776 + -0.7 19,776 + +0.0 19,776 + -0.5 19,776 + -0.7 19,776 + -0.3 19,777 - -0.1 19,777 - -2.2 19,777 - -1.2 19,777 - -0.4 19,777 - +0.1 19,777 - +1.1 19,777 - +0.6 20,169 + GFF5394 0.54 -1.4 20,169 + GFF5394 0.54 +0.6 20,169 + GFF5394 0.54 -0.9 20,169 + GFF5394 0.54 -0.8 20,380 - GFF5394 0.80 +0.2 20,380 - GFF5394 0.80 +0.5 20,380 - GFF5394 0.80 -0.7 20,678 + GFF5395 0.13 -0.2 20,678 + GFF5395 0.13 -0.0 20,678 + GFF5395 0.13 +0.8 20,678 + GFF5395 0.13 -0.9 20,678 + GFF5395 0.13 +0.5 20,678 + GFF5395 0.13 -1.2 20,678 + GFF5395 0.13 -1.2 20,678 + GFF5395 0.13 -0.6 20,678 + GFF5395 0.13 -1.5 20,678 + GFF5395 0.13 +0.3 20,678 + GFF5395 0.13 -0.0 20,678 + GFF5395 0.13 -0.4 20,678 + GFF5395 0.13 +1.4 20,678 + GFF5395 0.13 -0.8 20,678 + GFF5395 0.13 +0.2 20,679 - GFF5395 0.13 +2.1 20,679 - GFF5395 0.13 +1.3 20,679 - GFF5395 0.13 -1.7 20,679 - GFF5395 0.13 -0.2 20,679 - GFF5395 0.13 -0.5 20,679 - GFF5395 0.13 +0.0 20,679 - GFF5395 0.13 -0.8 20,679 - GFF5395 0.13 -0.4 20,679 - GFF5395 0.13 +0.6 20,679 - GFF5395 0.13 -1.0 20,679 - GFF5395 0.13 -0.1 20,679 - GFF5395 0.13 -0.6 20,679 - GFF5395 0.13 +0.1 20,679 - GFF5395 0.13 -0.7 21,044 + GFF5395 0.64 +0.3 21,044 + GFF5395 0.64 -1.9 21,044 + GFF5395 0.64 -0.5 21,044 + GFF5395 0.64 -0.7 21,044 + GFF5395 0.64 -0.1 21,044 + GFF5395 0.64 -0.8 21,044 + GFF5395 0.64 +0.1 21,044 + GFF5395 0.64 -1.5 21,045 - GFF5395 0.64 -0.3 21,045 - GFF5395 0.64 +0.8 21,045 - GFF5395 0.64 +0.1 21,045 - GFF5395 0.64 -1.2 21,045 - GFF5395 0.64 -0.9 21,045 - GFF5395 0.64 +0.1 21,045 - GFF5395 0.64 -0.1 21,045 - GFF5395 0.64 -0.2 21,366 + GFF5396 0.11 +1.1 21,366 + GFF5396 0.11 +1.1 21,366 + GFF5396 0.11 -1.8 21,367 - GFF5396 0.11 -0.4 21,367 - GFF5396 0.11 -0.7 21,367 - GFF5396 0.11 -2.6 21,390 + GFF5396 0.15 +0.9 21,390 + GFF5396 0.15 -0.2 21,435 + GFF5396 0.22 -2.0 21,435 + GFF5396 0.22 -0.7 21,435 + GFF5396 0.22 -0.9 21,435 + GFF5396 0.22 +0.1 21,435 + GFF5396 0.22 -0.2 21,435 + GFF5396 0.22 -0.5 21,435 + GFF5396 0.22 -0.9 21,436 - GFF5396 0.22 -0.6 21,436 - GFF5396 0.22 -0.7 21,436 - GFF5396 0.22 +0.3
Or see this region's nucleotide sequence