Strain Fitness in Variovorax sp. SCN45 around GFF5268

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF5267 and GFF5268 are separated by 141 nucleotidesGFF5268 and GFF5269 are separated by 67 nucleotides GFF5267 - Glucose-6-phosphate isomerase (EC 5.3.1.9), at 134,806 to 136,359 GFF5267 GFF5268 - protein of unknown function DUF805, at 136,501 to 136,833 GFF5268 GFF5269 - 4-hydroxythreonine-4-phosphate dehydrogenase (EC 1.1.1.262), at 136,901 to 137,950 GFF5269 Position (kb) 136 137Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 135.590 kb on - strand, within GFF5267at 135.940 kb on + strand, within GFF5267at 135.941 kb on - strand, within GFF5267at 135.941 kb on - strand, within GFF5267at 136.186 kb on + strand, within GFF5267at 136.187 kb on - strand, within GFF5267at 136.187 kb on - strand, within GFF5267at 136.187 kb on - strand, within GFF5267at 136.187 kb on - strand, within GFF5267at 136.382 kb on + strandat 136.383 kb on - strandat 136.383 kb on - strandat 136.383 kb on - strandat 136.386 kb on + strandat 136.387 kb on - strandat 136.387 kb on - strandat 136.387 kb on - strandat 136.387 kb on - strandat 136.419 kb on + strandat 136.425 kb on + strandat 136.425 kb on + strandat 136.426 kb on - strandat 136.426 kb on - strandat 136.426 kb on - strandat 136.426 kb on - strandat 136.426 kb on - strandat 136.451 kb on - strandat 136.498 kb on + strandat 136.498 kb on + strandat 136.499 kb on - strandat 136.540 kb on + strand, within GFF5268at 136.540 kb on + strand, within GFF5268at 136.541 kb on - strand, within GFF5268at 136.541 kb on - strand, within GFF5268at 136.576 kb on + strand, within GFF5268at 136.576 kb on + strand, within GFF5268at 136.576 kb on + strand, within GFF5268at 136.577 kb on - strand, within GFF5268at 136.577 kb on - strand, within GFF5268at 136.577 kb on - strand, within GFF5268at 136.577 kb on - strand, within GFF5268at 136.577 kb on - strand, within GFF5268at 136.636 kb on + strand, within GFF5268at 136.636 kb on + strand, within GFF5268at 136.636 kb on + strand, within GFF5268at 136.636 kb on + strand, within GFF5268at 136.636 kb on + strand, within GFF5268at 136.636 kb on + strand, within GFF5268at 136.636 kb on + strand, within GFF5268at 136.636 kb on + strand, within GFF5268at 136.636 kb on + strand, within GFF5268at 136.636 kb on + strand, within GFF5268at 136.636 kb on + strand, within GFF5268at 136.637 kb on - strand, within GFF5268at 136.637 kb on - strand, within GFF5268at 136.637 kb on - strand, within GFF5268at 136.637 kb on - strand, within GFF5268at 136.637 kb on - strand, within GFF5268at 136.637 kb on - strand, within GFF5268at 136.637 kb on - strand, within GFF5268at 136.637 kb on - strand, within GFF5268at 136.637 kb on - strand, within GFF5268at 136.637 kb on - strand, within GFF5268at 136.637 kb on - strand, within GFF5268at 136.637 kb on - strand, within GFF5268at 136.637 kb on - strand, within GFF5268at 136.637 kb on - strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.642 kb on + strand, within GFF5268at 136.643 kb on - strand, within GFF5268at 136.643 kb on - strand, within GFF5268at 136.643 kb on - strand, within GFF5268at 136.643 kb on - strand, within GFF5268at 136.643 kb on - strand, within GFF5268at 136.643 kb on - strand, within GFF5268at 136.771 kb on + strand, within GFF5268at 136.771 kb on + strand, within GFF5268at 136.771 kb on + strand, within GFF5268at 136.771 kb on + strand, within GFF5268at 136.772 kb on - strand, within GFF5268at 136.772 kb on - strand, within GFF5268at 136.772 kb on - strand, within GFF5268at 136.772 kb on - strand, within GFF5268at 136.772 kb on - strand, within GFF5268at 136.777 kb on + strand, within GFF5268at 136.777 kb on + strand, within GFF5268at 136.777 kb on + strand, within GFF5268at 136.778 kb on - strand, within GFF5268at 137.115 kb on + strand, within GFF5269at 137.115 kb on + strand, within GFF5269at 137.115 kb on + strand, within GFF5269at 137.116 kb on - strand, within GFF5269at 137.116 kb on - strand, within GFF5269at 137.116 kb on - strand, within GFF5269at 137.240 kb on + strand, within GFF5269at 137.240 kb on + strand, within GFF5269at 137.240 kb on + strand, within GFF5269at 137.240 kb on + strand, within GFF5269at 137.240 kb on + strand, within GFF5269at 137.240 kb on + strand, within GFF5269at 137.240 kb on + strand, within GFF5269at 137.241 kb on - strand, within GFF5269at 137.241 kb on - strand, within GFF5269at 137.241 kb on - strand, within GFF5269at 137.241 kb on - strand, within GFF5269at 137.493 kb on + strand, within GFF5269at 137.493 kb on + strand, within GFF5269at 137.494 kb on - strand, within GFF5269

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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135,590 - GFF5267 0.50 -1.1
135,940 + GFF5267 0.73 -1.8
135,941 - GFF5267 0.73 -3.3
135,941 - GFF5267 0.73 -2.1
136,186 + GFF5267 0.89 +0.3
136,187 - GFF5267 0.89 -1.8
136,187 - GFF5267 0.89 -1.6
136,187 - GFF5267 0.89 -2.4
136,187 - GFF5267 0.89 -0.1
136,382 + -1.2
136,383 - -0.0
136,383 - -0.8
136,383 - +0.5
136,386 + +0.5
136,387 - -0.6
136,387 - -0.5
136,387 - +0.9
136,387 - -0.7
136,419 + +0.3
136,425 + -0.5
136,425 + +2.1
136,426 - +0.2
136,426 - -0.2
136,426 - -0.7
136,426 - -0.2
136,426 - -2.1
136,451 - +1.1
136,498 + +1.6
136,498 + +0.6
136,499 - -1.7
136,540 + GFF5268 0.12 +0.1
136,540 + GFF5268 0.12 +0.5
136,541 - GFF5268 0.12 +1.9
136,541 - GFF5268 0.12 +0.1
136,576 + GFF5268 0.23 +0.2
136,576 + GFF5268 0.23 -0.5
136,576 + GFF5268 0.23 -0.0
136,577 - GFF5268 0.23 +0.1
136,577 - GFF5268 0.23 -0.1
136,577 - GFF5268 0.23 -1.1
136,577 - GFF5268 0.23 -0.5
136,577 - GFF5268 0.23 +0.2
136,636 + GFF5268 0.41 -0.1
136,636 + GFF5268 0.41 +2.3
136,636 + GFF5268 0.41 +1.5
136,636 + GFF5268 0.41 -1.5
136,636 + GFF5268 0.41 +0.2
136,636 + GFF5268 0.41 -0.8
136,636 + GFF5268 0.41 -0.1
136,636 + GFF5268 0.41 +0.6
136,636 + GFF5268 0.41 -0.2
136,636 + GFF5268 0.41 -0.1
136,636 + GFF5268 0.41 -0.6
136,637 - GFF5268 0.41 -0.9
136,637 - GFF5268 0.41 +0.3
136,637 - GFF5268 0.41 -0.1
136,637 - GFF5268 0.41 +0.5
136,637 - GFF5268 0.41 +0.2
136,637 - GFF5268 0.41 +0.3
136,637 - GFF5268 0.41 -0.0
136,637 - GFF5268 0.41 -0.2
136,637 - GFF5268 0.41 -0.6
136,637 - GFF5268 0.41 +1.9
136,637 - GFF5268 0.41 -1.0
136,637 - GFF5268 0.41 +1.7
136,637 - GFF5268 0.41 -1.2
136,637 - GFF5268 0.41 -0.7
136,642 + GFF5268 0.42 -0.0
136,642 + GFF5268 0.42 +0.8
136,642 + GFF5268 0.42 -0.2
136,642 + GFF5268 0.42 +0.4
136,642 + GFF5268 0.42 -0.3
136,642 + GFF5268 0.42 -1.0
136,642 + GFF5268 0.42 -0.6
136,642 + GFF5268 0.42 -0.3
136,642 + GFF5268 0.42 +0.5
136,642 + GFF5268 0.42 -0.5
136,642 + GFF5268 0.42 -1.6
136,642 + GFF5268 0.42 -0.5
136,642 + GFF5268 0.42 -0.9
136,642 + GFF5268 0.42 +1.5
136,642 + GFF5268 0.42 +0.1
136,642 + GFF5268 0.42 -0.5
136,642 + GFF5268 0.42 -0.2
136,642 + GFF5268 0.42 -0.6
136,642 + GFF5268 0.42 +0.0
136,642 + GFF5268 0.42 +1.1
136,642 + GFF5268 0.42 -1.2
136,642 + GFF5268 0.42 +0.2
136,642 + GFF5268 0.42 +0.2
136,642 + GFF5268 0.42 +0.1
136,643 - GFF5268 0.43 -0.1
136,643 - GFF5268 0.43 +0.1
136,643 - GFF5268 0.43 +0.4
136,643 - GFF5268 0.43 +2.3
136,643 - GFF5268 0.43 -1.1
136,643 - GFF5268 0.43 +0.3
136,771 + GFF5268 0.81 +0.3
136,771 + GFF5268 0.81 +0.1
136,771 + GFF5268 0.81 +0.5
136,771 + GFF5268 0.81 -0.7
136,772 - GFF5268 0.81 +2.1
136,772 - GFF5268 0.81 -0.7
136,772 - GFF5268 0.81 -0.0
136,772 - GFF5268 0.81 -0.5
136,772 - GFF5268 0.81 +1.0
136,777 + GFF5268 0.83 -0.1
136,777 + GFF5268 0.83 -1.5
136,777 + GFF5268 0.83 -1.4
136,778 - GFF5268 0.83 -0.0
137,115 + GFF5269 0.20 -0.4
137,115 + GFF5269 0.20 -0.5
137,115 + GFF5269 0.20 +0.2
137,116 - GFF5269 0.20 -0.3
137,116 - GFF5269 0.20 +1.2
137,116 - GFF5269 0.20 -0.2
137,240 + GFF5269 0.32 -0.5
137,240 + GFF5269 0.32 -1.1
137,240 + GFF5269 0.32 +0.5
137,240 + GFF5269 0.32 -0.8
137,240 + GFF5269 0.32 +0.5
137,240 + GFF5269 0.32 -0.9
137,240 + GFF5269 0.32 +0.3
137,241 - GFF5269 0.32 +0.9
137,241 - GFF5269 0.32 +2.9
137,241 - GFF5269 0.32 +0.1
137,241 - GFF5269 0.32 +1.4
137,493 + GFF5269 0.56 +1.1
137,493 + GFF5269 0.56 +0.1
137,494 - GFF5269 0.56 +0.1

Or see this region's nucleotide sequence