Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF5185 and GFF5186 overlap by 4 nucleotides GFF5186 and GFF5187 are separated by 27 nucleotides
GFF5185 - FIG00732904: hypothetical protein, at 38,479 to 43,239
GFF5185
GFF5186 - hypothetical protein, at 43,236 to 44,177
GFF5186
GFF5187 - Aldose 1-epimerase (EC 5.1.3.3), at 44,205 to 45,284
GFF5187
Position (kb)
38
39
40
41
42
43
44 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 37.534 kb on - strand at 37.534 kb on - strand at 37.681 kb on + strand at 37.681 kb on + strand at 37.719 kb on + strand at 37.720 kb on - strand at 37.720 kb on - strand at 37.720 kb on - strand at 37.790 kb on + strand at 37.905 kb on + strand at 37.931 kb on - strand at 38.053 kb on + strand at 38.149 kb on + strand at 38.150 kb on - strand at 38.150 kb on - strand at 38.150 kb on - strand at 38.152 kb on + strand at 38.159 kb on + strand at 38.160 kb on - strand at 38.265 kb on + strand at 38.265 kb on + strand at 38.266 kb on - strand at 38.307 kb on + strand at 38.308 kb on - strand at 38.815 kb on + strand at 38.816 kb on - strand at 38.816 kb on - strand at 38.816 kb on - strand at 38.896 kb on + strand at 38.896 kb on + strand at 38.896 kb on + strand at 38.896 kb on + strand at 38.896 kb on + strand at 38.897 kb on - strand at 38.897 kb on - strand at 39.031 kb on + strand, within GFF5185 at 39.031 kb on + strand, within GFF5185 at 39.031 kb on + strand, within GFF5185 at 39.031 kb on + strand, within GFF5185 at 39.032 kb on - strand, within GFF5185 at 39.032 kb on - strand, within GFF5185 at 39.032 kb on - strand, within GFF5185 at 39.148 kb on + strand, within GFF5185 at 39.148 kb on + strand, within GFF5185 at 39.148 kb on + strand, within GFF5185 at 39.148 kb on + strand, within GFF5185 at 39.149 kb on - strand, within GFF5185 at 39.187 kb on + strand, within GFF5185 at 39.187 kb on + strand, within GFF5185 at 39.188 kb on - strand, within GFF5185 at 39.188 kb on - strand, within GFF5185 at 39.188 kb on - strand, within GFF5185 at 39.226 kb on + strand, within GFF5185 at 39.226 kb on + strand, within GFF5185 at 39.226 kb on + strand, within GFF5185 at 39.226 kb on + strand, within GFF5185 at 39.226 kb on + strand, within GFF5185 at 39.227 kb on - strand, within GFF5185 at 39.256 kb on + strand, within GFF5185 at 39.257 kb on - strand, within GFF5185 at 39.257 kb on - strand, within GFF5185 at 39.257 kb on - strand, within GFF5185 at 39.385 kb on + strand, within GFF5185 at 39.454 kb on + strand, within GFF5185 at 39.454 kb on + strand, within GFF5185 at 39.454 kb on + strand, within GFF5185 at 39.454 kb on + strand, within GFF5185 at 39.454 kb on + strand, within GFF5185 at 39.455 kb on - strand, within GFF5185 at 39.455 kb on - strand, within GFF5185 at 39.455 kb on - strand, within GFF5185 at 39.455 kb on - strand, within GFF5185 at 39.455 kb on - strand, within GFF5185 at 39.604 kb on + strand, within GFF5185 at 39.604 kb on + strand, within GFF5185 at 39.605 kb on - strand, within GFF5185 at 39.605 kb on - strand, within GFF5185 at 39.605 kb on - strand, within GFF5185 at 39.605 kb on - strand, within GFF5185 at 39.607 kb on + strand, within GFF5185 at 39.608 kb on - strand, within GFF5185 at 39.608 kb on - strand, within GFF5185 at 39.644 kb on - strand, within GFF5185 at 39.745 kb on + strand, within GFF5185 at 39.787 kb on + strand, within GFF5185 at 39.976 kb on + strand, within GFF5185 at 39.977 kb on - strand, within GFF5185 at 39.977 kb on - strand, within GFF5185 at 39.977 kb on - strand, within GFF5185 at 40.111 kb on + strand, within GFF5185 at 40.111 kb on + strand, within GFF5185 at 40.111 kb on + strand, within GFF5185 at 40.112 kb on - strand, within GFF5185 at 40.124 kb on - strand, within GFF5185 at 40.124 kb on - strand, within GFF5185 at 40.124 kb on - strand, within GFF5185 at 40.132 kb on + strand, within GFF5185 at 40.132 kb on + strand, within GFF5185 at 40.381 kb on + strand, within GFF5185 at 40.382 kb on - strand, within GFF5185 at 40.382 kb on - strand, within GFF5185 at 40.382 kb on - strand, within GFF5185 at 40.382 kb on - strand, within GFF5185 at 40.384 kb on + strand, within GFF5185 at 40.384 kb on + strand, within GFF5185 at 40.384 kb on + strand, within GFF5185 at 40.385 kb on - strand, within GFF5185 at 40.385 kb on - strand, within GFF5185 at 40.472 kb on - strand, within GFF5185 at 40.888 kb on + strand, within GFF5185 at 41.545 kb on + strand, within GFF5185 at 41.926 kb on + strand, within GFF5185 at 41.927 kb on - strand, within GFF5185 at 41.953 kb on + strand, within GFF5185 at 41.954 kb on - strand, within GFF5185 at 42.133 kb on + strand, within GFF5185 at 42.134 kb on - strand, within GFF5185 at 42.244 kb on + strand, within GFF5185 at 42.244 kb on + strand, within GFF5185 at 42.245 kb on - strand, within GFF5185 at 42.245 kb on - strand, within GFF5185 at 42.245 kb on - strand, within GFF5185 at 42.245 kb on - strand, within GFF5185 at 42.445 kb on + strand, within GFF5185 at 42.446 kb on - strand, within GFF5185 at 42.667 kb on + strand, within GFF5185 at 43.138 kb on + strand at 43.235 kb on + strand at 43.235 kb on + strand at 43.235 kb on + strand at 43.235 kb on + strand at 43.236 kb on - strand at 43.236 kb on - strand at 43.236 kb on - strand at 43.236 kb on - strand at 43.236 kb on - strand at 43.236 kb on - strand at 43.236 kb on - strand at 43.419 kb on + strand, within GFF5186 at 43.419 kb on + strand, within GFF5186 at 43.420 kb on - strand, within GFF5186 at 43.650 kb on + strand, within GFF5186 at 43.650 kb on + strand, within GFF5186 at 43.651 kb on - strand, within GFF5186 at 43.651 kb on - strand, within GFF5186 at 43.651 kb on - strand, within GFF5186 at 43.929 kb on + strand, within GFF5186 at 43.929 kb on + strand, within GFF5186 at 43.930 kb on - strand, within GFF5186 at 43.930 kb on - strand, within GFF5186 at 44.106 kb on + strand at 44.200 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 37,534 - -0.3 37,534 - +0.1 37,681 + +0.3 37,681 + -1.0 37,719 + -1.7 37,720 - -2.4 37,720 - -1.4 37,720 - +0.3 37,790 + -1.6 37,905 + +1.4 37,931 - +0.3 38,053 + +0.4 38,149 + -0.3 38,150 - -0.3 38,150 - -0.3 38,150 - -0.8 38,152 + +0.8 38,159 + -0.7 38,160 - -0.7 38,265 + -0.5 38,265 + +0.9 38,266 - +1.0 38,307 + +2.9 38,308 - -2.8 38,815 + -0.6 38,816 - -0.7 38,816 - -0.2 38,816 - -0.1 38,896 + -0.8 38,896 + -0.7 38,896 + -0.6 38,896 + +1.0 38,896 + -0.4 38,897 - -0.5 38,897 - -2.8 39,031 + GFF5185 0.12 -0.0 39,031 + GFF5185 0.12 -0.3 39,031 + GFF5185 0.12 -0.5 39,031 + GFF5185 0.12 -0.2 39,032 - GFF5185 0.12 -0.2 39,032 - GFF5185 0.12 +0.1 39,032 - GFF5185 0.12 -0.0 39,148 + GFF5185 0.14 +0.4 39,148 + GFF5185 0.14 -0.3 39,148 + GFF5185 0.14 -0.1 39,148 + GFF5185 0.14 -0.6 39,149 - GFF5185 0.14 -1.7 39,187 + GFF5185 0.15 -0.5 39,187 + GFF5185 0.15 -1.2 39,188 - GFF5185 0.15 -0.7 39,188 - GFF5185 0.15 -0.6 39,188 - GFF5185 0.15 +0.3 39,226 + GFF5185 0.16 +0.6 39,226 + GFF5185 0.16 -0.6 39,226 + GFF5185 0.16 -0.2 39,226 + GFF5185 0.16 +0.3 39,226 + GFF5185 0.16 -0.1 39,227 - GFF5185 0.16 -0.4 39,256 + GFF5185 0.16 -0.5 39,257 - GFF5185 0.16 -0.8 39,257 - GFF5185 0.16 -0.1 39,257 - GFF5185 0.16 -1.6 39,385 + GFF5185 0.19 -1.7 39,454 + GFF5185 0.20 -0.8 39,454 + GFF5185 0.20 -0.5 39,454 + GFF5185 0.20 +0.4 39,454 + GFF5185 0.20 -0.7 39,454 + GFF5185 0.20 -0.5 39,455 - GFF5185 0.20 -0.2 39,455 - GFF5185 0.20 -0.4 39,455 - GFF5185 0.20 -2.9 39,455 - GFF5185 0.20 +0.4 39,455 - GFF5185 0.20 -0.9 39,604 + GFF5185 0.24 -0.8 39,604 + GFF5185 0.24 -0.1 39,605 - GFF5185 0.24 -0.0 39,605 - GFF5185 0.24 +0.1 39,605 - GFF5185 0.24 -0.5 39,605 - GFF5185 0.24 +0.6 39,607 + GFF5185 0.24 -0.2 39,608 - GFF5185 0.24 -0.0 39,608 - GFF5185 0.24 +0.5 39,644 - GFF5185 0.24 -1.3 39,745 + GFF5185 0.27 +1.0 39,787 + GFF5185 0.27 +0.9 39,976 + GFF5185 0.31 -0.5 39,977 - GFF5185 0.31 +0.6 39,977 - GFF5185 0.31 -1.3 39,977 - GFF5185 0.31 -0.5 40,111 + GFF5185 0.34 -0.5 40,111 + GFF5185 0.34 +0.5 40,111 + GFF5185 0.34 -1.1 40,112 - GFF5185 0.34 +0.1 40,124 - GFF5185 0.35 +0.0 40,124 - GFF5185 0.35 +1.2 40,124 - GFF5185 0.35 +0.1 40,132 + GFF5185 0.35 +0.2 40,132 + GFF5185 0.35 -0.6 40,381 + GFF5185 0.40 -1.1 40,382 - GFF5185 0.40 -0.2 40,382 - GFF5185 0.40 +0.1 40,382 - GFF5185 0.40 +0.0 40,382 - GFF5185 0.40 +0.1 40,384 + GFF5185 0.40 -0.6 40,384 + GFF5185 0.40 -1.5 40,384 + GFF5185 0.40 -0.5 40,385 - GFF5185 0.40 -0.3 40,385 - GFF5185 0.40 -0.5 40,472 - GFF5185 0.42 -0.1 40,888 + GFF5185 0.51 -1.7 41,545 + GFF5185 0.64 -0.8 41,926 + GFF5185 0.72 +0.3 41,927 - GFF5185 0.72 -0.3 41,953 + GFF5185 0.73 +0.3 41,954 - GFF5185 0.73 -0.1 42,133 + GFF5185 0.77 +0.1 42,134 - GFF5185 0.77 +0.5 42,244 + GFF5185 0.79 +0.5 42,244 + GFF5185 0.79 -0.1 42,245 - GFF5185 0.79 -1.7 42,245 - GFF5185 0.79 -1.1 42,245 - GFF5185 0.79 -1.3 42,245 - GFF5185 0.79 -1.1 42,445 + GFF5185 0.83 -0.4 42,446 - GFF5185 0.83 -0.4 42,667 + GFF5185 0.88 +0.5 43,138 + -0.8 43,235 + +0.7 43,235 + +0.2 43,235 + -0.2 43,235 + +0.2 43,236 - -1.6 43,236 - -0.2 43,236 - -0.7 43,236 - +0.1 43,236 - -0.5 43,236 - -0.7 43,236 - +0.9 43,419 + GFF5186 0.19 -0.5 43,419 + GFF5186 0.19 +3.2 43,420 - GFF5186 0.20 +0.4 43,650 + GFF5186 0.44 -0.9 43,650 + GFF5186 0.44 +0.9 43,651 - GFF5186 0.44 -1.7 43,651 - GFF5186 0.44 -0.3 43,651 - GFF5186 0.44 -0.5 43,929 + GFF5186 0.74 -1.2 43,929 + GFF5186 0.74 +1.5 43,930 - GFF5186 0.74 +0.0 43,930 - GFF5186 0.74 +0.1 44,106 + +0.9 44,200 + +0.3
Or see this region's nucleotide sequence