Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4793 and GFF4794 are separated by 4 nucleotides GFF4794 and GFF4795 overlap by 4 nucleotides GFF4795 and GFF4796 are separated by 116 nucleotides
GFF4793 - Homoserine dehydrogenase (EC 1.1.1.3), at 163,162 to 164,523
GFF4793
GFF4794 - Ectoine hydroxylase (EC 1.17.-.-), at 164,528 to 165,403
GFF4794
GFF4795 - predicted biotin regulatory protein BioR (GntR family), at 165,400 to 166,107
GFF4795
GFF4796 - L-threo-3-hydroxyaspartate ammonia-lyase (EC 4.3.1.16), at 166,224 to 167,183
GFF4796
Position (kb)
165
166
167 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 164.432 kb on + strand at 164.432 kb on + strand at 164.432 kb on + strand at 164.432 kb on + strand at 164.432 kb on + strand at 164.432 kb on + strand at 164.433 kb on - strand at 164.433 kb on - strand at 164.605 kb on - strand at 164.676 kb on + strand, within GFF4794 at 164.676 kb on + strand, within GFF4794 at 164.677 kb on - strand, within GFF4794 at 164.677 kb on - strand, within GFF4794 at 164.677 kb on - strand, within GFF4794 at 164.677 kb on - strand, within GFF4794 at 164.730 kb on + strand, within GFF4794 at 164.731 kb on - strand, within GFF4794 at 164.904 kb on + strand, within GFF4794 at 164.904 kb on + strand, within GFF4794 at 164.905 kb on - strand, within GFF4794 at 164.905 kb on - strand, within GFF4794 at 164.905 kb on - strand, within GFF4794 at 165.066 kb on + strand, within GFF4794 at 165.135 kb on + strand, within GFF4794 at 165.135 kb on + strand, within GFF4794 at 165.135 kb on + strand, within GFF4794 at 165.135 kb on + strand, within GFF4794 at 165.135 kb on + strand, within GFF4794 at 165.135 kb on + strand, within GFF4794 at 165.135 kb on + strand, within GFF4794 at 165.135 kb on + strand, within GFF4794 at 165.135 kb on + strand, within GFF4794 at 165.135 kb on + strand, within GFF4794 at 165.135 kb on + strand, within GFF4794 at 165.136 kb on - strand, within GFF4794 at 165.136 kb on - strand, within GFF4794 at 165.136 kb on - strand, within GFF4794 at 165.136 kb on - strand, within GFF4794 at 165.136 kb on - strand, within GFF4794 at 165.136 kb on - strand, within GFF4794 at 165.289 kb on - strand, within GFF4794 at 165.289 kb on - strand, within GFF4794 at 165.289 kb on - strand, within GFF4794 at 165.357 kb on + strand at 165.357 kb on + strand at 165.357 kb on + strand at 165.358 kb on - strand at 165.686 kb on + strand, within GFF4795 at 165.686 kb on + strand, within GFF4795 at 165.686 kb on + strand, within GFF4795 at 165.686 kb on + strand, within GFF4795 at 165.686 kb on + strand, within GFF4795 at 165.686 kb on + strand, within GFF4795 at 165.687 kb on - strand, within GFF4795 at 165.687 kb on - strand, within GFF4795 at 165.687 kb on - strand, within GFF4795 at 165.687 kb on - strand, within GFF4795 at 165.845 kb on + strand, within GFF4795 at 165.846 kb on - strand, within GFF4795 at 166.052 kb on + strand at 166.135 kb on + strand at 166.163 kb on + strand at 166.164 kb on - strand at 166.164 kb on - strand at 166.231 kb on + strand at 166.231 kb on + strand at 166.231 kb on + strand at 166.231 kb on + strand at 166.231 kb on + strand at 166.231 kb on + strand at 166.231 kb on + strand at 166.231 kb on + strand at 166.231 kb on + strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.232 kb on - strand at 166.235 kb on + strand at 166.235 kb on + strand at 166.235 kb on + strand at 166.235 kb on + strand at 166.235 kb on + strand at 166.235 kb on + strand at 166.236 kb on - strand at 166.236 kb on - strand at 166.236 kb on - strand at 166.236 kb on - strand at 166.236 kb on - strand at 166.239 kb on + strand at 166.239 kb on + strand at 166.239 kb on + strand at 166.239 kb on + strand at 166.240 kb on - strand at 166.240 kb on - strand at 166.240 kb on - strand at 166.240 kb on - strand at 166.240 kb on - strand at 166.567 kb on - strand, within GFF4796 at 166.567 kb on - strand, within GFF4796 at 166.587 kb on + strand, within GFF4796 at 166.587 kb on + strand, within GFF4796 at 166.587 kb on + strand, within GFF4796 at 166.587 kb on + strand, within GFF4796 at 166.587 kb on + strand, within GFF4796 at 166.588 kb on - strand, within GFF4796 at 166.588 kb on - strand, within GFF4796 at 166.666 kb on - strand, within GFF4796 at 166.666 kb on - strand, within GFF4796 at 166.666 kb on - strand, within GFF4796 at 166.824 kb on + strand, within GFF4796 at 166.825 kb on - strand, within GFF4796 at 166.944 kb on + strand, within GFF4796 at 166.944 kb on + strand, within GFF4796 at 166.944 kb on + strand, within GFF4796 at 166.944 kb on + strand, within GFF4796 at 166.945 kb on - strand, within GFF4796 at 166.945 kb on - strand, within GFF4796 at 166.945 kb on - strand, within GFF4796 at 166.945 kb on - strand, within GFF4796 at 166.945 kb on - strand, within GFF4796
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 164,432 + +0.1 164,432 + -1.1 164,432 + -0.2 164,432 + +0.4 164,432 + -0.4 164,432 + +0.2 164,433 - +1.0 164,433 - -1.6 164,605 - +0.6 164,676 + GFF4794 0.17 -1.5 164,676 + GFF4794 0.17 +1.1 164,677 - GFF4794 0.17 +0.3 164,677 - GFF4794 0.17 -0.2 164,677 - GFF4794 0.17 +2.6 164,677 - GFF4794 0.17 -0.2 164,730 + GFF4794 0.23 -0.7 164,731 - GFF4794 0.23 -0.2 164,904 + GFF4794 0.43 +0.3 164,904 + GFF4794 0.43 -0.6 164,905 - GFF4794 0.43 +0.2 164,905 - GFF4794 0.43 -0.4 164,905 - GFF4794 0.43 +0.4 165,066 + GFF4794 0.61 -1.0 165,135 + GFF4794 0.69 +1.1 165,135 + GFF4794 0.69 +0.5 165,135 + GFF4794 0.69 -0.0 165,135 + GFF4794 0.69 +0.6 165,135 + GFF4794 0.69 +0.8 165,135 + GFF4794 0.69 -1.0 165,135 + GFF4794 0.69 -0.8 165,135 + GFF4794 0.69 -1.4 165,135 + GFF4794 0.69 -0.3 165,135 + GFF4794 0.69 -0.2 165,135 + GFF4794 0.69 -1.0 165,136 - GFF4794 0.69 +0.6 165,136 - GFF4794 0.69 -1.8 165,136 - GFF4794 0.69 +1.2 165,136 - GFF4794 0.69 +1.6 165,136 - GFF4794 0.69 -1.0 165,136 - GFF4794 0.69 -0.2 165,289 - GFF4794 0.87 -0.2 165,289 - GFF4794 0.87 +1.1 165,289 - GFF4794 0.87 +0.6 165,357 + +0.7 165,357 + -1.1 165,357 + -1.2 165,358 - -0.2 165,686 + GFF4795 0.40 -1.3 165,686 + GFF4795 0.40 -0.3 165,686 + GFF4795 0.40 -1.0 165,686 + GFF4795 0.40 -0.3 165,686 + GFF4795 0.40 +1.0 165,686 + GFF4795 0.40 -2.2 165,687 - GFF4795 0.41 -1.6 165,687 - GFF4795 0.41 -0.1 165,687 - GFF4795 0.41 -0.3 165,687 - GFF4795 0.41 -1.8 165,845 + GFF4795 0.63 -0.0 165,846 - GFF4795 0.63 -0.0 166,052 + -2.0 166,135 + +0.2 166,163 + -1.0 166,164 - -0.4 166,164 - +1.0 166,231 + +1.1 166,231 + +1.0 166,231 + +0.8 166,231 + +0.6 166,231 + -1.1 166,231 + +0.6 166,231 + +0.8 166,231 + +0.1 166,231 + -0.2 166,232 - -0.4 166,232 - -0.8 166,232 - +0.6 166,232 - -1.8 166,232 - -0.7 166,232 - +0.1 166,232 - +0.6 166,232 - -0.8 166,232 - -0.0 166,232 - -0.1 166,232 - -2.1 166,232 - -0.3 166,232 - -1.9 166,232 - +0.8 166,235 + -2.8 166,235 + -0.1 166,235 + +0.1 166,235 + +0.3 166,235 + +1.1 166,235 + +0.2 166,236 - +0.1 166,236 - -1.1 166,236 - -2.1 166,236 - -0.8 166,236 - -1.3 166,239 + -0.0 166,239 + +0.6 166,239 + -0.2 166,239 + -0.4 166,240 - -0.5 166,240 - +0.1 166,240 - +1.6 166,240 - -1.9 166,240 - +0.1 166,567 - GFF4796 0.36 -0.1 166,567 - GFF4796 0.36 -0.0 166,587 + GFF4796 0.38 -0.4 166,587 + GFF4796 0.38 +0.1 166,587 + GFF4796 0.38 -1.4 166,587 + GFF4796 0.38 -0.2 166,587 + GFF4796 0.38 -2.0 166,588 - GFF4796 0.38 -1.0 166,588 - GFF4796 0.38 -0.0 166,666 - GFF4796 0.46 -0.7 166,666 - GFF4796 0.46 +0.9 166,666 - GFF4796 0.46 +0.1 166,824 + GFF4796 0.62 -1.5 166,825 - GFF4796 0.63 -0.6 166,944 + GFF4796 0.75 -0.2 166,944 + GFF4796 0.75 -1.0 166,944 + GFF4796 0.75 -0.3 166,944 + GFF4796 0.75 -0.8 166,945 - GFF4796 0.75 -1.2 166,945 - GFF4796 0.75 +0.1 166,945 - GFF4796 0.75 -0.7 166,945 - GFF4796 0.75 +0.2 166,945 - GFF4796 0.75 +0.1
Or see this region's nucleotide sequence