Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4731 and GFF4732 are separated by 48 nucleotides GFF4732 and GFF4733 are separated by 9 nucleotides GFF4733 and GFF4734 overlap by 4 nucleotides
GFF4731 - Assimilatory nitrate reductase large subunit (EC 1.7.99.4), at 94,717 to 97,509
GFF4731
GFF4732 - Nitrite reductase [NAD(P)H] small subunit (EC 1.7.1.4), at 97,558 to 97,953
GFF4732
GFF4733 - Nitric oxide -responding transcriptional regulator Dnr (Crp/Fnr family), at 97,963 to 98,754
GFF4733
GFF4734 - Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4), at 98,751 to 101,219
GFF4734
Position (kb) 97
98
99 Strain fitness (log2 ratio) -2
-1
0
1
2 at 97.029 kb on - strand, within GFF4731 at 97.067 kb on + strand, within GFF4731 at 97.068 kb on - strand, within GFF4731 at 97.157 kb on + strand, within GFF4731 at 97.157 kb on + strand, within GFF4731 at 97.157 kb on + strand, within GFF4731 at 97.158 kb on - strand, within GFF4731 at 97.158 kb on - strand, within GFF4731 at 97.158 kb on - strand, within GFF4731 at 97.160 kb on + strand, within GFF4731 at 97.160 kb on + strand, within GFF4731 at 97.160 kb on + strand, within GFF4731 at 97.160 kb on + strand, within GFF4731 at 97.160 kb on + strand, within GFF4731 at 97.160 kb on + strand, within GFF4731 at 97.161 kb on - strand, within GFF4731 at 97.161 kb on - strand, within GFF4731 at 97.161 kb on - strand, within GFF4731 at 97.161 kb on - strand, within GFF4731 at 97.161 kb on - strand, within GFF4731 at 97.161 kb on - strand, within GFF4731 at 97.190 kb on + strand, within GFF4731 at 97.191 kb on - strand, within GFF4731 at 97.481 kb on + strand at 97.560 kb on - strand at 97.964 kb on + strand at 97.964 kb on + strand at 97.965 kb on - strand at 97.965 kb on - strand at 97.965 kb on - strand at 97.965 kb on - strand at 97.982 kb on + strand at 97.983 kb on - strand at 98.258 kb on + strand, within GFF4733 at 98.259 kb on - strand, within GFF4733 at 98.345 kb on + strand, within GFF4733 at 98.480 kb on + strand, within GFF4733 at 98.481 kb on - strand, within GFF4733 at 98.609 kb on + strand, within GFF4733 at 98.609 kb on + strand, within GFF4733 at 98.610 kb on - strand, within GFF4733 at 98.610 kb on - strand, within GFF4733 at 98.921 kb on - strand at 98.944 kb on + strand at 98.944 kb on + strand at 98.944 kb on + strand at 98.945 kb on - strand at 98.945 kb on - strand at 98.965 kb on + strand at 98.966 kb on - strand at 99.007 kb on + strand, within GFF4734 at 99.008 kb on - strand, within GFF4734 at 99.008 kb on - strand, within GFF4734 at 99.084 kb on + strand, within GFF4734 at 99.084 kb on + strand, within GFF4734 at 99.085 kb on - strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.088 kb on + strand, within GFF4734 at 99.089 kb on - strand, within GFF4734 at 99.089 kb on - strand, within GFF4734 at 99.196 kb on + strand, within GFF4734 at 99.196 kb on + strand, within GFF4734 at 99.197 kb on - strand, within GFF4734 at 99.311 kb on - strand, within GFF4734 at 99.424 kb on + strand, within GFF4734 at 99.502 kb on + strand, within GFF4734 at 99.502 kb on + strand, within GFF4734 at 99.502 kb on + strand, within GFF4734 at 99.502 kb on + strand, within GFF4734 at 99.503 kb on - strand, within GFF4734 at 99.503 kb on - strand, within GFF4734 at 99.598 kb on + strand, within GFF4734 at 99.598 kb on + strand, within GFF4734 at 99.599 kb on - strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.601 kb on + strand, within GFF4734 at 99.602 kb on - strand, within GFF4734 at 99.602 kb on - strand, within GFF4734 at 99.682 kb on + strand, within GFF4734 at 99.682 kb on + strand, within GFF4734
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 97,029 - GFF4731 0.83 -0.5 97,067 + GFF4731 0.84 +1.3 97,068 - GFF4731 0.84 -0.3 97,157 + GFF4731 0.87 +1.7 97,157 + GFF4731 0.87 -1.9 97,157 + GFF4731 0.87 -0.1 97,158 - GFF4731 0.87 -0.1 97,158 - GFF4731 0.87 -0.4 97,158 - GFF4731 0.87 -0.0 97,160 + GFF4731 0.87 -0.4 97,160 + GFF4731 0.87 +0.4 97,160 + GFF4731 0.87 -0.8 97,160 + GFF4731 0.87 -0.3 97,160 + GFF4731 0.87 -0.0 97,160 + GFF4731 0.87 +0.1 97,161 - GFF4731 0.88 -0.5 97,161 - GFF4731 0.88 -1.2 97,161 - GFF4731 0.88 -1.1 97,161 - GFF4731 0.88 -0.2 97,161 - GFF4731 0.88 -0.5 97,161 - GFF4731 0.88 -0.6 97,190 + GFF4731 0.89 +0.2 97,191 - GFF4731 0.89 +0.2 97,481 + -2.1 97,560 - -0.5 97,964 + -1.0 97,964 + -0.0 97,965 - +0.6 97,965 - -0.6 97,965 - -1.0 97,965 - +0.7 97,982 + +0.2 97,983 - +1.0 98,258 + GFF4733 0.37 -0.0 98,259 - GFF4733 0.37 +0.6 98,345 + GFF4733 0.48 +0.2 98,480 + GFF4733 0.65 -0.6 98,481 - GFF4733 0.65 +1.6 98,609 + GFF4733 0.82 +1.6 98,609 + GFF4733 0.82 +0.0 98,610 - GFF4733 0.82 -1.4 98,610 - GFF4733 0.82 +2.0 98,921 - -0.4 98,944 + -0.2 98,944 + +1.9 98,944 + -1.4 98,945 - +1.0 98,945 - +1.6 98,965 + -1.1 98,966 - +1.9 99,007 + GFF4734 0.10 -0.0 99,008 - GFF4734 0.10 -0.5 99,008 - GFF4734 0.10 -0.3 99,084 + GFF4734 0.13 +0.0 99,084 + GFF4734 0.13 +0.1 99,085 - GFF4734 0.14 +0.6 99,088 + GFF4734 0.14 -1.3 99,088 + GFF4734 0.14 -0.9 99,088 + GFF4734 0.14 -1.0 99,088 + GFF4734 0.14 -0.3 99,088 + GFF4734 0.14 +1.0 99,088 + GFF4734 0.14 -1.0 99,089 - GFF4734 0.14 -1.0 99,089 - GFF4734 0.14 -0.5 99,196 + GFF4734 0.18 +0.6 99,196 + GFF4734 0.18 -0.3 99,197 - GFF4734 0.18 -0.1 99,311 - GFF4734 0.23 -0.6 99,424 + GFF4734 0.27 -0.0 99,502 + GFF4734 0.30 -0.4 99,502 + GFF4734 0.30 -0.7 99,502 + GFF4734 0.30 -0.3 99,502 + GFF4734 0.30 -2.3 99,503 - GFF4734 0.30 -1.0 99,503 - GFF4734 0.30 +1.2 99,598 + GFF4734 0.34 -0.5 99,598 + GFF4734 0.34 +1.6 99,599 - GFF4734 0.34 -1.5 99,601 + GFF4734 0.34 -0.0 99,601 + GFF4734 0.34 -1.1 99,601 + GFF4734 0.34 -1.0 99,601 + GFF4734 0.34 -0.2 99,601 + GFF4734 0.34 -0.0 99,601 + GFF4734 0.34 -1.4 99,601 + GFF4734 0.34 +0.8 99,602 - GFF4734 0.34 -0.6 99,602 - GFF4734 0.34 -0.2 99,682 + GFF4734 0.38 +0.2 99,682 + GFF4734 0.38 -0.2
Or see this region's nucleotide sequence