Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4700 and GFF4701 overlap by 4 nucleotides GFF4701 and GFF4702 overlap by 4 nucleotides GFF4702 and GFF4703 are separated by 29 nucleotides
GFF4700 - Various polyols ABC transporter, permease protein 1, at 64,008 to 64,892
GFF4700
GFF4701 - Various polyols ABC transporter, permease protein 2, at 64,889 to 65,731
GFF4701
GFF4702 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 65,728 to 66,867
GFF4702
GFF4703 - Xylitol dehydrogenase (EC 1.1.1.9), at 66,897 to 67,946
GFF4703
Position (kb)
65
66
67 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 64.767 kb on + strand, within GFF4700 at 64.767 kb on + strand, within GFF4700 at 64.767 kb on + strand, within GFF4700 at 64.767 kb on + strand, within GFF4700 at 64.767 kb on + strand, within GFF4700 at 64.767 kb on + strand, within GFF4700 at 64.767 kb on + strand, within GFF4700 at 64.767 kb on + strand, within GFF4700 at 64.767 kb on + strand, within GFF4700 at 64.767 kb on + strand, within GFF4700 at 64.767 kb on + strand, within GFF4700 at 64.767 kb on + strand, within GFF4700 at 64.767 kb on + strand, within GFF4700 at 64.768 kb on - strand, within GFF4700 at 64.768 kb on - strand, within GFF4700 at 64.768 kb on - strand, within GFF4700 at 64.768 kb on - strand, within GFF4700 at 64.768 kb on - strand, within GFF4700 at 64.768 kb on - strand, within GFF4700 at 64.768 kb on - strand, within GFF4700 at 64.768 kb on - strand, within GFF4700 at 64.768 kb on - strand, within GFF4700 at 64.768 kb on - strand, within GFF4700 at 64.768 kb on - strand, within GFF4700 at 64.803 kb on - strand, within GFF4700 at 64.809 kb on + strand at 64.809 kb on + strand at 64.809 kb on + strand at 64.809 kb on + strand at 64.809 kb on + strand at 64.810 kb on - strand at 64.810 kb on - strand at 64.810 kb on - strand at 64.810 kb on - strand at 64.810 kb on - strand at 64.863 kb on + strand at 64.863 kb on + strand at 64.863 kb on + strand at 64.863 kb on + strand at 64.863 kb on + strand at 64.863 kb on + strand at 64.863 kb on + strand at 64.863 kb on + strand at 64.863 kb on + strand at 64.864 kb on - strand at 64.864 kb on - strand at 64.864 kb on - strand at 64.864 kb on - strand at 64.864 kb on - strand at 64.864 kb on - strand at 64.864 kb on - strand at 64.864 kb on - strand at 64.864 kb on - strand at 64.864 kb on - strand at 65.069 kb on + strand, within GFF4701 at 65.069 kb on + strand, within GFF4701 at 65.069 kb on + strand, within GFF4701 at 65.069 kb on + strand, within GFF4701 at 65.069 kb on + strand, within GFF4701 at 65.069 kb on + strand, within GFF4701 at 65.069 kb on + strand, within GFF4701 at 65.069 kb on + strand, within GFF4701 at 65.069 kb on + strand, within GFF4701 at 65.069 kb on + strand, within GFF4701 at 65.069 kb on + strand, within GFF4701 at 65.070 kb on - strand, within GFF4701 at 65.070 kb on - strand, within GFF4701 at 65.070 kb on - strand, within GFF4701 at 65.070 kb on - strand, within GFF4701 at 65.070 kb on - strand, within GFF4701 at 65.070 kb on - strand, within GFF4701 at 65.070 kb on - strand, within GFF4701 at 65.070 kb on - strand, within GFF4701 at 65.070 kb on - strand, within GFF4701 at 65.075 kb on + strand, within GFF4701 at 65.075 kb on + strand, within GFF4701 at 65.075 kb on + strand, within GFF4701 at 65.075 kb on + strand, within GFF4701 at 65.075 kb on + strand, within GFF4701 at 65.075 kb on + strand, within GFF4701 at 65.075 kb on + strand, within GFF4701 at 65.075 kb on + strand, within GFF4701 at 65.075 kb on + strand, within GFF4701 at 65.075 kb on + strand, within GFF4701 at 65.075 kb on + strand, within GFF4701 at 65.076 kb on - strand, within GFF4701 at 65.076 kb on - strand, within GFF4701 at 65.076 kb on - strand, within GFF4701 at 65.076 kb on - strand, within GFF4701 at 65.076 kb on - strand, within GFF4701 at 65.076 kb on - strand, within GFF4701 at 65.076 kb on - strand, within GFF4701 at 65.076 kb on - strand, within GFF4701 at 65.076 kb on - strand, within GFF4701 at 65.117 kb on + strand, within GFF4701 at 65.178 kb on - strand, within GFF4701 at 65.303 kb on + strand, within GFF4701 at 65.304 kb on - strand, within GFF4701 at 65.304 kb on - strand, within GFF4701 at 65.304 kb on - strand, within GFF4701 at 65.304 kb on - strand, within GFF4701 at 65.333 kb on + strand, within GFF4701 at 65.333 kb on + strand, within GFF4701 at 65.334 kb on - strand, within GFF4701 at 65.373 kb on + strand, within GFF4701 at 65.374 kb on - strand, within GFF4701 at 65.439 kb on - strand, within GFF4701 at 66.013 kb on + strand, within GFF4702 at 66.013 kb on + strand, within GFF4702 at 66.014 kb on - strand, within GFF4702 at 66.023 kb on - strand, within GFF4702 at 66.328 kb on + strand, within GFF4702 at 66.328 kb on + strand, within GFF4702 at 66.328 kb on + strand, within GFF4702 at 66.328 kb on + strand, within GFF4702 at 66.328 kb on + strand, within GFF4702 at 66.328 kb on + strand, within GFF4702 at 66.328 kb on + strand, within GFF4702 at 66.329 kb on - strand, within GFF4702 at 66.329 kb on - strand, within GFF4702 at 66.329 kb on - strand, within GFF4702 at 66.329 kb on - strand, within GFF4702 at 66.682 kb on + strand, within GFF4702 at 67.026 kb on + strand, within GFF4703 at 67.026 kb on + strand, within GFF4703 at 67.027 kb on - strand, within GFF4703 at 67.027 kb on - strand, within GFF4703 at 67.027 kb on - strand, within GFF4703 at 67.030 kb on - strand, within GFF4703 at 67.030 kb on - strand, within GFF4703 at 67.053 kb on + strand, within GFF4703 at 67.053 kb on + strand, within GFF4703 at 67.054 kb on - strand, within GFF4703 at 67.054 kb on - strand, within GFF4703 at 67.054 kb on - strand, within GFF4703 at 67.195 kb on + strand, within GFF4703 at 67.195 kb on + strand, within GFF4703 at 67.195 kb on + strand, within GFF4703 at 67.196 kb on - strand, within GFF4703 at 67.196 kb on - strand, within GFF4703 at 67.202 kb on + strand, within GFF4703 at 67.202 kb on + strand, within GFF4703 at 67.202 kb on + strand, within GFF4703 at 67.202 kb on + strand, within GFF4703 at 67.202 kb on + strand, within GFF4703 at 67.202 kb on + strand, within GFF4703 at 67.203 kb on - strand, within GFF4703 at 67.203 kb on - strand, within GFF4703 at 67.206 kb on + strand, within GFF4703 at 67.206 kb on + strand, within GFF4703 at 67.206 kb on + strand, within GFF4703 at 67.206 kb on + strand, within GFF4703 at 67.206 kb on + strand, within GFF4703 at 67.206 kb on + strand, within GFF4703 at 67.206 kb on + strand, within GFF4703 at 67.206 kb on + strand, within GFF4703 at 67.206 kb on + strand, within GFF4703 at 67.207 kb on - strand, within GFF4703 at 67.207 kb on - strand, within GFF4703 at 67.207 kb on - strand, within GFF4703 at 67.207 kb on - strand, within GFF4703 at 67.207 kb on - strand, within GFF4703 at 67.207 kb on - strand, within GFF4703 at 67.694 kb on - strand, within GFF4703
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 64,767 + GFF4700 0.86 +0.1 64,767 + GFF4700 0.86 +0.5 64,767 + GFF4700 0.86 -1.4 64,767 + GFF4700 0.86 +0.1 64,767 + GFF4700 0.86 -0.3 64,767 + GFF4700 0.86 +0.4 64,767 + GFF4700 0.86 -2.0 64,767 + GFF4700 0.86 +0.2 64,767 + GFF4700 0.86 +0.4 64,767 + GFF4700 0.86 -2.1 64,767 + GFF4700 0.86 +0.8 64,767 + GFF4700 0.86 -0.4 64,767 + GFF4700 0.86 +0.2 64,768 - GFF4700 0.86 +0.1 64,768 - GFF4700 0.86 -0.3 64,768 - GFF4700 0.86 -0.8 64,768 - GFF4700 0.86 -0.7 64,768 - GFF4700 0.86 +0.6 64,768 - GFF4700 0.86 -0.9 64,768 - GFF4700 0.86 -2.0 64,768 - GFF4700 0.86 -1.4 64,768 - GFF4700 0.86 -1.2 64,768 - GFF4700 0.86 -0.9 64,768 - GFF4700 0.86 -0.6 64,803 - GFF4700 0.90 +0.8 64,809 + +1.1 64,809 + -0.6 64,809 + -2.0 64,809 + -0.0 64,809 + -0.7 64,810 - -0.6 64,810 - -1.4 64,810 - -1.9 64,810 - -1.5 64,810 - -0.8 64,863 + -1.0 64,863 + -1.4 64,863 + +1.1 64,863 + -0.3 64,863 + +0.8 64,863 + -2.2 64,863 + +0.2 64,863 + +0.9 64,863 + +0.2 64,864 - -1.6 64,864 - -1.5 64,864 - +0.1 64,864 - -1.3 64,864 - +1.6 64,864 - +1.2 64,864 - +0.6 64,864 - -0.2 64,864 - -0.2 64,864 - -0.1 65,069 + GFF4701 0.21 +0.7 65,069 + GFF4701 0.21 -0.0 65,069 + GFF4701 0.21 +0.2 65,069 + GFF4701 0.21 +0.1 65,069 + GFF4701 0.21 +1.1 65,069 + GFF4701 0.21 -1.1 65,069 + GFF4701 0.21 +0.7 65,069 + GFF4701 0.21 +1.1 65,069 + GFF4701 0.21 -0.0 65,069 + GFF4701 0.21 -0.5 65,069 + GFF4701 0.21 +0.7 65,070 - GFF4701 0.21 -0.1 65,070 - GFF4701 0.21 -0.7 65,070 - GFF4701 0.21 -0.5 65,070 - GFF4701 0.21 -0.6 65,070 - GFF4701 0.21 -1.6 65,070 - GFF4701 0.21 -1.2 65,070 - GFF4701 0.21 -0.4 65,070 - GFF4701 0.21 -0.8 65,070 - GFF4701 0.21 -0.2 65,075 + GFF4701 0.22 +0.3 65,075 + GFF4701 0.22 -0.1 65,075 + GFF4701 0.22 -0.3 65,075 + GFF4701 0.22 +0.6 65,075 + GFF4701 0.22 -2.1 65,075 + GFF4701 0.22 -0.5 65,075 + GFF4701 0.22 +1.1 65,075 + GFF4701 0.22 -0.2 65,075 + GFF4701 0.22 +0.1 65,075 + GFF4701 0.22 -3.4 65,075 + GFF4701 0.22 -1.6 65,076 - GFF4701 0.22 -0.5 65,076 - GFF4701 0.22 -0.4 65,076 - GFF4701 0.22 -0.1 65,076 - GFF4701 0.22 +1.0 65,076 - GFF4701 0.22 -0.5 65,076 - GFF4701 0.22 -0.5 65,076 - GFF4701 0.22 +0.6 65,076 - GFF4701 0.22 -0.7 65,076 - GFF4701 0.22 -0.3 65,117 + GFF4701 0.27 -0.4 65,178 - GFF4701 0.34 +0.2 65,303 + GFF4701 0.49 -0.7 65,304 - GFF4701 0.49 +0.4 65,304 - GFF4701 0.49 -2.0 65,304 - GFF4701 0.49 -0.8 65,304 - GFF4701 0.49 -3.0 65,333 + GFF4701 0.53 +0.6 65,333 + GFF4701 0.53 -0.6 65,334 - GFF4701 0.53 -0.5 65,373 + GFF4701 0.57 -0.6 65,374 - GFF4701 0.58 +0.0 65,439 - GFF4701 0.65 -0.1 66,013 + GFF4702 0.25 +0.4 66,013 + GFF4702 0.25 +0.2 66,014 - GFF4702 0.25 -0.0 66,023 - GFF4702 0.26 +1.6 66,328 + GFF4702 0.53 -0.0 66,328 + GFF4702 0.53 -0.7 66,328 + GFF4702 0.53 -0.8 66,328 + GFF4702 0.53 +0.1 66,328 + GFF4702 0.53 +1.1 66,328 + GFF4702 0.53 +0.1 66,328 + GFF4702 0.53 -0.3 66,329 - GFF4702 0.53 +0.7 66,329 - GFF4702 0.53 +0.2 66,329 - GFF4702 0.53 +0.1 66,329 - GFF4702 0.53 +0.5 66,682 + GFF4702 0.84 +1.8 67,026 + GFF4703 0.12 -0.1 67,026 + GFF4703 0.12 -0.1 67,027 - GFF4703 0.12 -0.1 67,027 - GFF4703 0.12 -0.3 67,027 - GFF4703 0.12 -0.2 67,030 - GFF4703 0.13 -0.6 67,030 - GFF4703 0.13 -1.2 67,053 + GFF4703 0.15 +1.6 67,053 + GFF4703 0.15 +0.3 67,054 - GFF4703 0.15 +0.4 67,054 - GFF4703 0.15 -0.0 67,054 - GFF4703 0.15 -0.2 67,195 + GFF4703 0.28 +0.2 67,195 + GFF4703 0.28 -0.8 67,195 + GFF4703 0.28 +0.3 67,196 - GFF4703 0.28 -1.8 67,196 - GFF4703 0.28 -1.3 67,202 + GFF4703 0.29 -1.9 67,202 + GFF4703 0.29 -0.1 67,202 + GFF4703 0.29 -0.7 67,202 + GFF4703 0.29 +0.8 67,202 + GFF4703 0.29 +0.0 67,202 + GFF4703 0.29 -0.8 67,203 - GFF4703 0.29 -1.1 67,203 - GFF4703 0.29 -0.5 67,206 + GFF4703 0.29 -0.6 67,206 + GFF4703 0.29 -0.1 67,206 + GFF4703 0.29 -0.5 67,206 + GFF4703 0.29 -0.3 67,206 + GFF4703 0.29 +0.7 67,206 + GFF4703 0.29 +0.4 67,206 + GFF4703 0.29 -0.1 67,206 + GFF4703 0.29 -0.2 67,206 + GFF4703 0.29 -1.1 67,207 - GFF4703 0.30 -0.2 67,207 - GFF4703 0.30 -1.0 67,207 - GFF4703 0.30 -1.1 67,207 - GFF4703 0.30 -0.1 67,207 - GFF4703 0.30 -1.1 67,207 - GFF4703 0.30 -2.1 67,694 - GFF4703 0.76 -0.6
Or see this region's nucleotide sequence