Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4674 and GFF4675 are separated by 3 nucleotides GFF4675 and GFF4676 are separated by 11 nucleotides GFF4676 and GFF4677 are separated by 131 nucleotides
GFF4674 - Amidohydrolase family protein, at 31,850 to 33,190
GFF4674
GFF4675 - no description, at 33,194 to 33,754
GFF4675
GFF4676 - hypothetical protein, at 33,766 to 34,902
GFF4676
GFF4677 - Small-conductance mechanosensitive channel, at 35,034 to 36,113
GFF4677
Position (kb) 33
34
35 Strain fitness (log2 ratio) -2
-1
0
1
2 at 32.783 kb on + strand, within GFF4674 at 32.784 kb on - strand, within GFF4674 at 32.784 kb on - strand, within GFF4674 at 32.888 kb on + strand, within GFF4674 at 32.888 kb on + strand, within GFF4674 at 32.888 kb on + strand, within GFF4674 at 32.888 kb on + strand, within GFF4674 at 32.888 kb on + strand, within GFF4674 at 32.888 kb on + strand, within GFF4674 at 32.888 kb on + strand, within GFF4674 at 32.888 kb on + strand, within GFF4674 at 32.889 kb on - strand, within GFF4674 at 33.011 kb on + strand, within GFF4674 at 33.011 kb on + strand, within GFF4674 at 33.011 kb on + strand, within GFF4674 at 33.012 kb on - strand, within GFF4674 at 33.012 kb on - strand, within GFF4674 at 33.107 kb on + strand at 33.107 kb on + strand at 33.107 kb on + strand at 33.108 kb on - strand at 33.108 kb on - strand at 33.108 kb on - strand at 33.108 kb on - strand at 33.108 kb on - strand at 33.108 kb on - strand at 33.108 kb on - strand at 33.108 kb on - strand at 33.108 kb on - strand at 33.167 kb on + strand at 33.167 kb on + strand at 33.167 kb on + strand at 33.167 kb on + strand at 33.167 kb on + strand at 33.167 kb on + strand at 33.168 kb on - strand at 33.168 kb on - strand at 33.168 kb on - strand at 33.168 kb on - strand at 33.168 kb on - strand at 33.188 kb on + strand at 33.188 kb on + strand at 33.188 kb on + strand at 33.188 kb on + strand at 33.189 kb on - strand at 33.189 kb on - strand at 33.297 kb on + strand, within GFF4675 at 33.297 kb on + strand, within GFF4675 at 33.297 kb on + strand, within GFF4675 at 33.297 kb on + strand, within GFF4675 at 33.297 kb on + strand, within GFF4675 at 33.297 kb on + strand, within GFF4675 at 33.297 kb on + strand, within GFF4675 at 33.297 kb on + strand, within GFF4675 at 33.297 kb on + strand, within GFF4675 at 33.298 kb on - strand, within GFF4675 at 33.298 kb on - strand, within GFF4675 at 33.298 kb on - strand, within GFF4675 at 33.313 kb on + strand, within GFF4675 at 33.313 kb on + strand, within GFF4675 at 33.314 kb on - strand, within GFF4675 at 33.404 kb on + strand, within GFF4675 at 33.404 kb on + strand, within GFF4675 at 33.405 kb on - strand, within GFF4675 at 33.405 kb on - strand, within GFF4675 at 33.552 kb on + strand, within GFF4675 at 33.553 kb on - strand, within GFF4675 at 33.575 kb on - strand, within GFF4675 at 33.638 kb on + strand, within GFF4675 at 33.639 kb on - strand, within GFF4675 at 33.643 kb on + strand, within GFF4675 at 33.643 kb on + strand, within GFF4675 at 33.643 kb on + strand, within GFF4675 at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.767 kb on + strand at 33.768 kb on - strand at 33.768 kb on - strand at 33.768 kb on - strand at 33.768 kb on - strand at 33.768 kb on - strand at 33.768 kb on - strand at 33.768 kb on - strand at 33.768 kb on - strand at 33.768 kb on - strand at 33.768 kb on - strand at 33.768 kb on - strand at 33.828 kb on + strand at 33.828 kb on + strand at 33.829 kb on - strand at 33.829 kb on - strand at 33.933 kb on + strand, within GFF4676 at 33.933 kb on + strand, within GFF4676 at 33.933 kb on + strand, within GFF4676 at 33.933 kb on + strand, within GFF4676 at 33.933 kb on + strand, within GFF4676 at 33.933 kb on + strand, within GFF4676 at 33.934 kb on - strand, within GFF4676 at 33.934 kb on - strand, within GFF4676 at 33.934 kb on - strand, within GFF4676 at 33.934 kb on - strand, within GFF4676 at 33.934 kb on - strand, within GFF4676 at 33.934 kb on - strand, within GFF4676 at 33.934 kb on - strand, within GFF4676 at 34.040 kb on + strand, within GFF4676 at 34.040 kb on + strand, within GFF4676 at 34.041 kb on - strand, within GFF4676 at 34.041 kb on - strand, within GFF4676 at 34.041 kb on - strand, within GFF4676 at 34.151 kb on + strand, within GFF4676 at 34.151 kb on + strand, within GFF4676 at 34.152 kb on - strand, within GFF4676 at 34.152 kb on - strand, within GFF4676 at 34.152 kb on - strand, within GFF4676 at 34.226 kb on + strand, within GFF4676 at 34.227 kb on - strand, within GFF4676 at 34.331 kb on + strand, within GFF4676 at 34.331 kb on + strand, within GFF4676 at 34.331 kb on + strand, within GFF4676 at 34.331 kb on + strand, within GFF4676 at 34.331 kb on + strand, within GFF4676 at 34.331 kb on + strand, within GFF4676 at 34.331 kb on + strand, within GFF4676 at 34.331 kb on + strand, within GFF4676 at 34.331 kb on + strand, within GFF4676 at 34.331 kb on + strand, within GFF4676 at 34.331 kb on + strand, within GFF4676 at 34.331 kb on + strand, within GFF4676 at 34.332 kb on - strand, within GFF4676 at 34.332 kb on - strand, within GFF4676 at 34.332 kb on - strand, within GFF4676 at 34.332 kb on - strand, within GFF4676 at 34.332 kb on - strand, within GFF4676 at 34.332 kb on - strand, within GFF4676 at 34.523 kb on + strand, within GFF4676 at 34.524 kb on - strand, within GFF4676 at 34.524 kb on - strand, within GFF4676 at 34.524 kb on - strand, within GFF4676 at 34.524 kb on - strand, within GFF4676 at 34.524 kb on - strand, within GFF4676 at 34.524 kb on - strand, within GFF4676 at 34.524 kb on - strand, within GFF4676 at 34.526 kb on + strand, within GFF4676 at 34.526 kb on + strand, within GFF4676 at 34.527 kb on - strand, within GFF4676 at 34.527 kb on - strand, within GFF4676 at 34.627 kb on + strand, within GFF4676 at 34.627 kb on + strand, within GFF4676 at 34.627 kb on + strand, within GFF4676 at 34.627 kb on + strand, within GFF4676 at 34.627 kb on + strand, within GFF4676 at 34.628 kb on - strand, within GFF4676 at 34.628 kb on - strand, within GFF4676 at 34.628 kb on - strand, within GFF4676 at 34.628 kb on - strand, within GFF4676 at 34.694 kb on + strand, within GFF4676 at 34.694 kb on + strand, within GFF4676 at 34.694 kb on + strand, within GFF4676 at 34.694 kb on + strand, within GFF4676 at 34.694 kb on + strand, within GFF4676 at 34.694 kb on + strand, within GFF4676 at 34.694 kb on + strand, within GFF4676 at 34.694 kb on + strand, within GFF4676 at 34.694 kb on + strand, within GFF4676 at 34.695 kb on - strand, within GFF4676 at 34.695 kb on - strand, within GFF4676 at 34.695 kb on - strand, within GFF4676 at 34.695 kb on - strand, within GFF4676 at 34.695 kb on - strand, within GFF4676 at 34.695 kb on - strand, within GFF4676 at 34.695 kb on - strand, within GFF4676 at 34.697 kb on + strand, within GFF4676 at 34.697 kb on + strand, within GFF4676 at 34.697 kb on + strand, within GFF4676 at 34.697 kb on + strand, within GFF4676 at 34.697 kb on + strand, within GFF4676 at 34.698 kb on - strand, within GFF4676 at 34.698 kb on - strand, within GFF4676 at 34.698 kb on - strand, within GFF4676 at 34.698 kb on - strand, within GFF4676 at 34.698 kb on - strand, within GFF4676 at 34.698 kb on - strand, within GFF4676 at 34.698 kb on - strand, within GFF4676 at 34.698 kb on - strand, within GFF4676 at 34.698 kb on - strand, within GFF4676 at 34.698 kb on - strand, within GFF4676 at 34.728 kb on - strand, within GFF4676 at 34.728 kb on - strand, within GFF4676 at 34.728 kb on - strand, within GFF4676 at 34.804 kb on + strand at 34.805 kb on + strand at 34.805 kb on + strand at 34.805 kb on + strand at 34.805 kb on + strand at 34.805 kb on + strand at 34.805 kb on + strand at 34.805 kb on + strand at 34.806 kb on - strand at 34.806 kb on - strand at 34.958 kb on + strand at 34.972 kb on + strand at 34.972 kb on + strand at 35.330 kb on - strand, within GFF4677 at 35.338 kb on + strand, within GFF4677 at 35.407 kb on + strand, within GFF4677 at 35.407 kb on + strand, within GFF4677 at 35.407 kb on + strand, within GFF4677 at 35.408 kb on - strand, within GFF4677 at 35.408 kb on - strand, within GFF4677 at 35.408 kb on - strand, within GFF4677 at 35.408 kb on - strand, within GFF4677 at 35.408 kb on - strand, within GFF4677 at 35.408 kb on - strand, within GFF4677 at 35.408 kb on - strand, within GFF4677 at 35.408 kb on - strand, within GFF4677 at 35.408 kb on - strand, within GFF4677 at 35.408 kb on - strand, within GFF4677
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 32,783 + GFF4674 0.70 -2.5 32,784 - GFF4674 0.70 +0.8 32,784 - GFF4674 0.70 +0.4 32,888 + GFF4674 0.77 -1.5 32,888 + GFF4674 0.77 +0.3 32,888 + GFF4674 0.77 -0.0 32,888 + GFF4674 0.77 -0.4 32,888 + GFF4674 0.77 -0.2 32,888 + GFF4674 0.77 -1.3 32,888 + GFF4674 0.77 +0.2 32,888 + GFF4674 0.77 -0.9 32,889 - GFF4674 0.77 +0.1 33,011 + GFF4674 0.87 -1.8 33,011 + GFF4674 0.87 +0.1 33,011 + GFF4674 0.87 -0.1 33,012 - GFF4674 0.87 +0.8 33,012 - GFF4674 0.87 +0.1 33,107 + -1.0 33,107 + +0.6 33,107 + -1.5 33,108 - -0.2 33,108 - -0.1 33,108 - +0.0 33,108 - -0.9 33,108 - -0.6 33,108 - -0.2 33,108 - -1.9 33,108 - +1.0 33,108 - -0.5 33,167 + -0.9 33,167 + -0.4 33,167 + -1.4 33,167 + -0.4 33,167 + -0.6 33,167 + -0.6 33,168 - -0.9 33,168 - -0.7 33,168 - -0.4 33,168 - -0.3 33,168 - +0.3 33,188 + +0.8 33,188 + -0.4 33,188 + -1.2 33,188 + +0.4 33,189 - +0.6 33,189 - -2.0 33,297 + GFF4675 0.18 -1.5 33,297 + GFF4675 0.18 -0.1 33,297 + GFF4675 0.18 -0.8 33,297 + GFF4675 0.18 -1.1 33,297 + GFF4675 0.18 -0.0 33,297 + GFF4675 0.18 -1.2 33,297 + GFF4675 0.18 -0.8 33,297 + GFF4675 0.18 +0.6 33,297 + GFF4675 0.18 +0.4 33,298 - GFF4675 0.19 -1.1 33,298 - GFF4675 0.19 +0.1 33,298 - GFF4675 0.19 -0.7 33,313 + GFF4675 0.21 -1.0 33,313 + GFF4675 0.21 -2.7 33,314 - GFF4675 0.21 -0.1 33,404 + GFF4675 0.37 +0.4 33,404 + GFF4675 0.37 -1.2 33,405 - GFF4675 0.38 -1.5 33,405 - GFF4675 0.38 -0.0 33,552 + GFF4675 0.64 -0.4 33,553 - GFF4675 0.64 +0.8 33,575 - GFF4675 0.68 -0.9 33,638 + GFF4675 0.79 -1.0 33,639 - GFF4675 0.79 +0.7 33,643 + GFF4675 0.80 +0.1 33,643 + GFF4675 0.80 -1.0 33,643 + GFF4675 0.80 -1.0 33,767 + +0.8 33,767 + -0.0 33,767 + +0.7 33,767 + +0.7 33,767 + -0.4 33,767 + +0.0 33,767 + +0.1 33,767 + -1.3 33,767 + -0.4 33,767 + -0.1 33,767 + -0.6 33,767 + +0.1 33,767 + -0.7 33,767 + -1.0 33,767 + -1.9 33,767 + -0.8 33,767 + +0.0 33,767 + -0.4 33,767 + -0.4 33,768 - -0.2 33,768 - -0.7 33,768 - +0.6 33,768 - -1.4 33,768 - +0.4 33,768 - -0.8 33,768 - +0.4 33,768 - -0.0 33,768 - +0.0 33,768 - -0.2 33,768 - -1.1 33,828 + +0.3 33,828 + -0.3 33,829 - +0.6 33,829 - -0.3 33,933 + GFF4676 0.15 -0.2 33,933 + GFF4676 0.15 -0.2 33,933 + GFF4676 0.15 -1.3 33,933 + GFF4676 0.15 -0.5 33,933 + GFF4676 0.15 -1.0 33,933 + GFF4676 0.15 -0.7 33,934 - GFF4676 0.15 +2.2 33,934 - GFF4676 0.15 -0.8 33,934 - GFF4676 0.15 +0.3 33,934 - GFF4676 0.15 +0.1 33,934 - GFF4676 0.15 -0.4 33,934 - GFF4676 0.15 +0.6 33,934 - GFF4676 0.15 +1.0 34,040 + GFF4676 0.24 +0.6 34,040 + GFF4676 0.24 +0.3 34,041 - GFF4676 0.24 +0.2 34,041 - GFF4676 0.24 -0.7 34,041 - GFF4676 0.24 +0.1 34,151 + GFF4676 0.34 +0.6 34,151 + GFF4676 0.34 +0.3 34,152 - GFF4676 0.34 -1.4 34,152 - GFF4676 0.34 -1.6 34,152 - GFF4676 0.34 -0.8 34,226 + GFF4676 0.40 +0.8 34,227 - GFF4676 0.41 -0.1 34,331 + GFF4676 0.50 +1.1 34,331 + GFF4676 0.50 +0.7 34,331 + GFF4676 0.50 -2.0 34,331 + GFF4676 0.50 -0.6 34,331 + GFF4676 0.50 -1.1 34,331 + GFF4676 0.50 -2.1 34,331 + GFF4676 0.50 +0.1 34,331 + GFF4676 0.50 +0.0 34,331 + GFF4676 0.50 -0.6 34,331 + GFF4676 0.50 -0.2 34,331 + GFF4676 0.50 +0.5 34,331 + GFF4676 0.50 -0.8 34,332 - GFF4676 0.50 +0.2 34,332 - GFF4676 0.50 +2.1 34,332 - GFF4676 0.50 -0.4 34,332 - GFF4676 0.50 -0.6 34,332 - GFF4676 0.50 -1.0 34,332 - GFF4676 0.50 -2.1 34,523 + GFF4676 0.67 -2.2 34,524 - GFF4676 0.67 +0.8 34,524 - GFF4676 0.67 +1.0 34,524 - GFF4676 0.67 -1.0 34,524 - GFF4676 0.67 -0.9 34,524 - GFF4676 0.67 -1.4 34,524 - GFF4676 0.67 -0.4 34,524 - GFF4676 0.67 -0.4 34,526 + GFF4676 0.67 -0.4 34,526 + GFF4676 0.67 -0.2 34,527 - GFF4676 0.67 -1.2 34,527 - GFF4676 0.67 -0.3 34,627 + GFF4676 0.76 -1.1 34,627 + GFF4676 0.76 -0.3 34,627 + GFF4676 0.76 -0.8 34,627 + GFF4676 0.76 -0.1 34,627 + GFF4676 0.76 -0.1 34,628 - GFF4676 0.76 -1.7 34,628 - GFF4676 0.76 +0.6 34,628 - GFF4676 0.76 -0.3 34,628 - GFF4676 0.76 -0.4 34,694 + GFF4676 0.82 -0.4 34,694 + GFF4676 0.82 -2.1 34,694 + GFF4676 0.82 -0.0 34,694 + GFF4676 0.82 -0.9 34,694 + GFF4676 0.82 -0.9 34,694 + GFF4676 0.82 -0.7 34,694 + GFF4676 0.82 -0.7 34,694 + GFF4676 0.82 -0.7 34,694 + GFF4676 0.82 -1.0 34,695 - GFF4676 0.82 -0.3 34,695 - GFF4676 0.82 -0.4 34,695 - GFF4676 0.82 -0.7 34,695 - GFF4676 0.82 -0.0 34,695 - GFF4676 0.82 +0.3 34,695 - GFF4676 0.82 -0.2 34,695 - GFF4676 0.82 -0.1 34,697 + GFF4676 0.82 +0.1 34,697 + GFF4676 0.82 +0.6 34,697 + GFF4676 0.82 +0.2 34,697 + GFF4676 0.82 +0.8 34,697 + GFF4676 0.82 +0.1 34,698 - GFF4676 0.82 -0.1 34,698 - GFF4676 0.82 -1.0 34,698 - GFF4676 0.82 +0.1 34,698 - GFF4676 0.82 -0.4 34,698 - GFF4676 0.82 -0.4 34,698 - GFF4676 0.82 -0.1 34,698 - GFF4676 0.82 -0.4 34,698 - GFF4676 0.82 -0.4 34,698 - GFF4676 0.82 +0.1 34,698 - GFF4676 0.82 -0.5 34,728 - GFF4676 0.85 -0.7 34,728 - GFF4676 0.85 -0.6 34,728 - GFF4676 0.85 -0.4 34,804 + -0.3 34,805 + +0.2 34,805 + -0.0 34,805 + -0.1 34,805 + -1.0 34,805 + -0.8 34,805 + -0.4 34,805 + +0.4 34,806 - -0.1 34,806 - -0.3 34,958 + -0.5 34,972 + -0.4 34,972 + -0.0 35,330 - GFF4677 0.27 -0.6 35,338 + GFF4677 0.28 -1.8 35,407 + GFF4677 0.35 +0.8 35,407 + GFF4677 0.35 -0.6 35,407 + GFF4677 0.35 -2.0 35,408 - GFF4677 0.35 -0.4 35,408 - GFF4677 0.35 +0.1 35,408 - GFF4677 0.35 +0.1 35,408 - GFF4677 0.35 +0.0 35,408 - GFF4677 0.35 -0.4 35,408 - GFF4677 0.35 -1.0 35,408 - GFF4677 0.35 +0.1 35,408 - GFF4677 0.35 -0.7 35,408 - GFF4677 0.35 +0.3 35,408 - GFF4677 0.35 -1.1
Or see this region's nucleotide sequence