Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4671 and GFF4672 are separated by 47 nucleotides GFF4672 and GFF4673 are separated by 34 nucleotides GFF4673 and GFF4674 are separated by 150 nucleotides
GFF4671 - putative membrane protein, at 27,956 to 29,167
GFF4671
GFF4672 - Exoenzymes regulatory protein AepA precursor, at 29,215 to 30,900
GFF4672
GFF4673 - hypothetical protein, at 30,935 to 31,699
GFF4673
GFF4674 - Amidohydrolase family protein, at 31,850 to 33,190
GFF4674
Position (kb)
29
30
31 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 28.294 kb on - strand, within GFF4671 at 28.301 kb on + strand, within GFF4671 at 28.302 kb on - strand, within GFF4671 at 28.325 kb on + strand, within GFF4671 at 28.325 kb on + strand, within GFF4671 at 28.325 kb on + strand, within GFF4671 at 28.326 kb on - strand, within GFF4671 at 28.440 kb on - strand, within GFF4671 at 28.829 kb on + strand, within GFF4671 at 28.829 kb on + strand, within GFF4671 at 28.829 kb on + strand, within GFF4671 at 28.830 kb on - strand, within GFF4671 at 28.922 kb on + strand, within GFF4671 at 28.922 kb on + strand, within GFF4671 at 28.922 kb on + strand, within GFF4671 at 28.923 kb on - strand, within GFF4671 at 29.076 kb on - strand at 29.169 kb on - strand at 29.169 kb on - strand at 29.237 kb on + strand at 29.237 kb on + strand at 29.238 kb on - strand at 29.388 kb on - strand, within GFF4672 at 29.588 kb on + strand, within GFF4672 at 29.588 kb on + strand, within GFF4672 at 29.588 kb on + strand, within GFF4672 at 29.589 kb on - strand, within GFF4672 at 29.589 kb on - strand, within GFF4672 at 29.589 kb on - strand, within GFF4672 at 29.772 kb on - strand, within GFF4672 at 29.841 kb on - strand, within GFF4672 at 30.015 kb on - strand, within GFF4672 at 30.050 kb on + strand, within GFF4672 at 30.051 kb on - strand, within GFF4672 at 30.081 kb on - strand, within GFF4672 at 30.122 kb on + strand, within GFF4672 at 30.537 kb on - strand, within GFF4672 at 30.693 kb on - strand, within GFF4672 at 30.693 kb on - strand, within GFF4672 at 30.791 kb on + strand at 30.791 kb on + strand at 30.791 kb on + strand at 30.792 kb on - strand at 30.792 kb on - strand at 30.792 kb on - strand at 30.816 kb on + strand at 30.817 kb on - strand at 30.906 kb on + strand at 30.906 kb on + strand at 30.906 kb on + strand at 30.907 kb on - strand at 30.907 kb on - strand at 30.907 kb on - strand at 30.990 kb on + strand at 30.990 kb on + strand at 30.991 kb on - strand at 30.991 kb on - strand at 30.991 kb on - strand at 31.005 kb on + strand at 31.005 kb on + strand at 31.005 kb on + strand at 31.005 kb on + strand at 31.005 kb on + strand at 31.006 kb on - strand at 31.006 kb on - strand at 31.006 kb on - strand at 31.006 kb on - strand at 31.006 kb on - strand at 31.163 kb on + strand, within GFF4673 at 31.263 kb on + strand, within GFF4673 at 31.263 kb on + strand, within GFF4673 at 31.263 kb on + strand, within GFF4673 at 31.263 kb on + strand, within GFF4673 at 31.263 kb on + strand, within GFF4673 at 31.263 kb on + strand, within GFF4673 at 31.264 kb on - strand, within GFF4673 at 31.264 kb on - strand, within GFF4673 at 31.264 kb on - strand, within GFF4673 at 31.264 kb on - strand, within GFF4673 at 31.264 kb on - strand, within GFF4673 at 31.264 kb on - strand, within GFF4673 at 31.276 kb on - strand, within GFF4673 at 31.276 kb on - strand, within GFF4673 at 31.276 kb on - strand, within GFF4673 at 31.467 kb on + strand, within GFF4673 at 31.467 kb on + strand, within GFF4673 at 31.467 kb on + strand, within GFF4673 at 31.468 kb on - strand, within GFF4673 at 31.468 kb on - strand, within GFF4673 at 31.468 kb on - strand, within GFF4673 at 31.468 kb on - strand, within GFF4673 at 31.468 kb on - strand, within GFF4673 at 31.563 kb on + strand, within GFF4673 at 31.563 kb on + strand, within GFF4673 at 31.563 kb on + strand, within GFF4673 at 31.563 kb on + strand, within GFF4673 at 31.563 kb on + strand, within GFF4673 at 31.563 kb on + strand, within GFF4673 at 31.563 kb on + strand, within GFF4673 at 31.564 kb on - strand, within GFF4673 at 31.564 kb on - strand, within GFF4673 at 31.564 kb on - strand, within GFF4673 at 31.572 kb on + strand, within GFF4673 at 31.573 kb on - strand, within GFF4673 at 31.573 kb on - strand, within GFF4673 at 31.573 kb on - strand, within GFF4673 at 31.573 kb on - strand, within GFF4673 at 31.720 kb on + strand at 31.720 kb on + strand at 31.720 kb on + strand at 31.721 kb on - strand at 31.772 kb on + strand at 31.772 kb on + strand at 31.772 kb on + strand at 31.772 kb on + strand at 31.772 kb on + strand at 31.772 kb on + strand at 31.772 kb on + strand at 31.772 kb on + strand at 31.772 kb on + strand at 31.772 kb on + strand at 31.772 kb on + strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.773 kb on - strand at 31.793 kb on + strand at 31.793 kb on + strand at 31.793 kb on + strand at 31.794 kb on - strand at 31.794 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 28,294 - GFF4671 0.28 +0.1 28,301 + GFF4671 0.28 -0.2 28,302 - GFF4671 0.29 -0.4 28,325 + GFF4671 0.30 -0.7 28,325 + GFF4671 0.30 +0.4 28,325 + GFF4671 0.30 -1.9 28,326 - GFF4671 0.31 -1.3 28,440 - GFF4671 0.40 -0.9 28,829 + GFF4671 0.72 +0.4 28,829 + GFF4671 0.72 -0.9 28,829 + GFF4671 0.72 -0.5 28,830 - GFF4671 0.72 +0.2 28,922 + GFF4671 0.80 -0.4 28,922 + GFF4671 0.80 +0.6 28,922 + GFF4671 0.80 +0.2 28,923 - GFF4671 0.80 -0.4 29,076 - +2.1 29,169 - -0.1 29,169 - -0.2 29,237 + -0.4 29,237 + -0.4 29,238 - -0.6 29,388 - GFF4672 0.10 -0.6 29,588 + GFF4672 0.22 +0.6 29,588 + GFF4672 0.22 -1.3 29,588 + GFF4672 0.22 +0.4 29,589 - GFF4672 0.22 -0.6 29,589 - GFF4672 0.22 -0.0 29,589 - GFF4672 0.22 -2.6 29,772 - GFF4672 0.33 +0.1 29,841 - GFF4672 0.37 -0.2 30,015 - GFF4672 0.47 -0.8 30,050 + GFF4672 0.50 -0.2 30,051 - GFF4672 0.50 -0.7 30,081 - GFF4672 0.51 -0.4 30,122 + GFF4672 0.54 +0.6 30,537 - GFF4672 0.78 -1.1 30,693 - GFF4672 0.88 -0.2 30,693 - GFF4672 0.88 -0.2 30,791 + -2.2 30,791 + -0.1 30,791 + -1.2 30,792 - +0.7 30,792 - -1.0 30,792 - +0.1 30,816 + -0.2 30,817 - -0.3 30,906 + -0.1 30,906 + +0.1 30,906 + +0.6 30,907 - -0.0 30,907 - +0.6 30,907 - -1.2 30,990 + +0.5 30,990 + +0.0 30,991 - -0.6 30,991 - +0.8 30,991 - -0.6 31,005 + -0.7 31,005 + -0.0 31,005 + -2.5 31,005 + -0.9 31,005 + +0.2 31,006 - -0.6 31,006 - +0.3 31,006 - +0.8 31,006 - -0.7 31,006 - -3.5 31,163 + GFF4673 0.30 +1.3 31,263 + GFF4673 0.43 +0.7 31,263 + GFF4673 0.43 -0.7 31,263 + GFF4673 0.43 -1.5 31,263 + GFF4673 0.43 -0.6 31,263 + GFF4673 0.43 -0.1 31,263 + GFF4673 0.43 +0.2 31,264 - GFF4673 0.43 +0.6 31,264 - GFF4673 0.43 -0.8 31,264 - GFF4673 0.43 +0.6 31,264 - GFF4673 0.43 -0.3 31,264 - GFF4673 0.43 -0.4 31,264 - GFF4673 0.43 +0.6 31,276 - GFF4673 0.45 +0.2 31,276 - GFF4673 0.45 -0.3 31,276 - GFF4673 0.45 +0.6 31,467 + GFF4673 0.70 -2.4 31,467 + GFF4673 0.70 -0.4 31,467 + GFF4673 0.70 -0.4 31,468 - GFF4673 0.70 -0.2 31,468 - GFF4673 0.70 +0.1 31,468 - GFF4673 0.70 -0.7 31,468 - GFF4673 0.70 -0.1 31,468 - GFF4673 0.70 +0.7 31,563 + GFF4673 0.82 -1.6 31,563 + GFF4673 0.82 -0.6 31,563 + GFF4673 0.82 +1.2 31,563 + GFF4673 0.82 -0.5 31,563 + GFF4673 0.82 +0.7 31,563 + GFF4673 0.82 -1.2 31,563 + GFF4673 0.82 +0.7 31,564 - GFF4673 0.82 -0.0 31,564 - GFF4673 0.82 -0.5 31,564 - GFF4673 0.82 -2.6 31,572 + GFF4673 0.83 -0.2 31,573 - GFF4673 0.83 +0.3 31,573 - GFF4673 0.83 -0.9 31,573 - GFF4673 0.83 -0.3 31,573 - GFF4673 0.83 -0.8 31,720 + -0.4 31,720 + +0.2 31,720 + +0.6 31,721 - +1.1 31,772 + -2.2 31,772 + +1.6 31,772 + +0.4 31,772 + -0.3 31,772 + +0.2 31,772 + -0.3 31,772 + -0.9 31,772 + -0.9 31,772 + +0.1 31,772 + +0.3 31,772 + +1.3 31,773 - +1.1 31,773 - -0.4 31,773 - -0.1 31,773 - -0.1 31,773 - -0.2 31,773 - -0.1 31,773 - +0.2 31,773 - -1.0 31,773 - +1.0 31,773 - -1.0 31,773 - -0.5 31,773 - -0.0 31,773 - -0.6 31,773 - -0.1 31,773 - -0.0 31,773 - -1.4 31,773 - -0.9 31,773 - -0.6 31,793 + +0.3 31,793 + -0.3 31,793 + -1.9 31,794 - -1.1 31,794 - +1.6
Or see this region's nucleotide sequence