Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4613 and GFF4614 are separated by 9 nucleotides GFF4614 and GFF4615 are separated by 27 nucleotides GFF4615 and GFF4616 are separated by 26 nucleotides GFF4616 and GFF4617 overlap by 4 nucleotides
GFF4613 - Transcriptional regulator, LysR family, at 255,539 to 256,426
GFF4613
GFF4614 - Transcriptional regulator, AraC family, at 256,436 to 257,287
GFF4614
GFF4615 - 5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase, at 257,315 to 258,163
GFF4615
GFF4616 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 258,190 to 258,936
GFF4616
GFF4617 - 2-keto-3-deoxy-L-fuconate dehydrogenase, at 258,933 to 259,667
GFF4617
Position (kb) 257
258
259 Strain fitness (log2 ratio) -2
-1
0
1 at 256.346 kb on + strand at 256.346 kb on + strand at 256.347 kb on - strand at 256.347 kb on - strand at 256.347 kb on - strand at 256.347 kb on - strand at 256.347 kb on - strand at 256.347 kb on - strand at 256.347 kb on - strand at 256.353 kb on - strand at 256.353 kb on - strand at 256.398 kb on - strand at 256.398 kb on - strand at 256.398 kb on - strand at 256.438 kb on - strand at 257.020 kb on - strand, within GFF4614 at 257.020 kb on - strand, within GFF4614 at 257.107 kb on - strand, within GFF4614 at 257.172 kb on + strand, within GFF4614 at 257.173 kb on - strand, within GFF4614 at 257.196 kb on + strand, within GFF4614 at 257.196 kb on + strand, within GFF4614 at 257.487 kb on + strand, within GFF4615 at 257.488 kb on - strand, within GFF4615 at 257.488 kb on - strand, within GFF4615 at 257.716 kb on - strand, within GFF4615 at 257.728 kb on - strand, within GFF4615 at 257.754 kb on + strand, within GFF4615 at 257.755 kb on - strand, within GFF4615 at 257.755 kb on - strand, within GFF4615 at 257.925 kb on + strand, within GFF4615 at 257.925 kb on + strand, within GFF4615 at 257.925 kb on + strand, within GFF4615 at 257.926 kb on - strand, within GFF4615 at 257.926 kb on - strand, within GFF4615 at 257.926 kb on - strand, within GFF4615 at 257.926 kb on - strand, within GFF4615 at 258.194 kb on + strand at 258.194 kb on + strand at 258.195 kb on - strand at 258.195 kb on - strand at 258.195 kb on - strand at 258.195 kb on - strand at 258.195 kb on - strand at 258.195 kb on - strand at 258.195 kb on - strand at 258.195 kb on - strand at 258.195 kb on - strand at 258.195 kb on - strand at 258.537 kb on - strand, within GFF4616 at 258.936 kb on + strand at 258.937 kb on - strand at 258.937 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 256,346 + +0.6 256,346 + +1.0 256,347 - -0.1 256,347 - +0.2 256,347 - -0.3 256,347 - +0.8 256,347 - -1.1 256,347 - +0.9 256,347 - -0.1 256,353 - +0.6 256,353 - +0.8 256,398 - -1.0 256,398 - -1.4 256,398 - +0.1 256,438 - -0.0 257,020 - GFF4614 0.69 -0.1 257,020 - GFF4614 0.69 +0.7 257,107 - GFF4614 0.79 +0.0 257,172 + GFF4614 0.86 -0.3 257,173 - GFF4614 0.87 -1.0 257,196 + GFF4614 0.89 -1.1 257,196 + GFF4614 0.89 -1.1 257,487 + GFF4615 0.20 -1.4 257,488 - GFF4615 0.20 -1.7 257,488 - GFF4615 0.20 +1.2 257,716 - GFF4615 0.47 -0.1 257,728 - GFF4615 0.49 -0.7 257,754 + GFF4615 0.52 -0.7 257,755 - GFF4615 0.52 +0.2 257,755 - GFF4615 0.52 -0.9 257,925 + GFF4615 0.72 -0.7 257,925 + GFF4615 0.72 +0.2 257,925 + GFF4615 0.72 -0.1 257,926 - GFF4615 0.72 -0.7 257,926 - GFF4615 0.72 -0.7 257,926 - GFF4615 0.72 -0.8 257,926 - GFF4615 0.72 -1.0 258,194 + +0.7 258,194 + +0.6 258,195 - -0.0 258,195 - -0.4 258,195 - -0.1 258,195 - -1.1 258,195 - +0.6 258,195 - -2.0 258,195 - -1.3 258,195 - +0.0 258,195 - +0.1 258,195 - -0.7 258,537 - GFF4616 0.46 +0.9 258,936 + -1.3 258,937 - -1.8 258,937 - +0.9
Or see this region's nucleotide sequence