Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4461 and GFF4462 are separated by 66 nucleotides GFF4462 and GFF4463 are separated by 1 nucleotides GFF4463 and GFF4464 are separated by 0 nucleotides GFF4464 and GFF4465 are separated by 48 nucleotides
GFF4461 - hypothetical protein, at 101,487 to 103,649
GFF4461
GFF4462 - Crossover junction endodeoxyribonuclease RuvC (EC 3.1.22.4), at 103,716 to 104,264
GFF4462
GFF4463 - alpha-methyl-L-serine aldolase, at 104,266 to 105,534
GFF4463
GFF4464 - hypothetical protein, at 105,535 to 105,660
GFF4464
GFF4465 - Transcriptional regulator, LysR family, at 105,709 to 106,665
GFF4465
Position (kb) 104
105
106 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 103.267 kb on + strand, within GFF4461 at 103.563 kb on + strand at 103.563 kb on + strand at 103.564 kb on - strand at 103.619 kb on - strand at 103.702 kb on + strand at 103.711 kb on + strand at 103.711 kb on + strand at 103.711 kb on + strand at 103.711 kb on + strand at 103.712 kb on - strand at 103.712 kb on - strand at 103.712 kb on - strand at 103.712 kb on - strand at 103.712 kb on - strand at 103.712 kb on - strand at 103.795 kb on - strand, within GFF4462 at 104.025 kb on + strand, within GFF4462 at 104.241 kb on + strand at 104.241 kb on + strand at 104.279 kb on + strand at 104.279 kb on + strand at 104.279 kb on + strand at 104.280 kb on - strand at 104.280 kb on - strand at 104.280 kb on - strand at 104.280 kb on - strand at 104.660 kb on + strand, within GFF4463 at 104.660 kb on + strand, within GFF4463 at 104.660 kb on + strand, within GFF4463 at 104.661 kb on - strand, within GFF4463 at 104.661 kb on - strand, within GFF4463 at 104.828 kb on + strand, within GFF4463 at 104.828 kb on + strand, within GFF4463 at 104.828 kb on + strand, within GFF4463 at 104.828 kb on + strand, within GFF4463 at 104.829 kb on - strand, within GFF4463 at 104.829 kb on - strand, within GFF4463 at 104.829 kb on - strand, within GFF4463 at 105.062 kb on + strand, within GFF4463 at 105.206 kb on + strand, within GFF4463 at 105.206 kb on + strand, within GFF4463 at 105.207 kb on - strand, within GFF4463 at 105.207 kb on - strand, within GFF4463 at 105.326 kb on + strand, within GFF4463 at 105.326 kb on + strand, within GFF4463 at 105.327 kb on - strand, within GFF4463 at 105.327 kb on - strand, within GFF4463 at 105.327 kb on - strand, within GFF4463 at 105.903 kb on - strand, within GFF4465 at 105.903 kb on - strand, within GFF4465 at 105.903 kb on - strand, within GFF4465 at 105.903 kb on - strand, within GFF4465 at 106.175 kb on - strand, within GFF4465 at 106.453 kb on + strand, within GFF4465 at 106.453 kb on + strand, within GFF4465
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 103,267 + GFF4461 0.82 -0.4 103,563 + -0.3 103,563 + -0.9 103,564 - +3.1 103,619 - +0.0 103,702 + -0.3 103,711 + +0.3 103,711 + +0.2 103,711 + -0.9 103,711 + -1.1 103,712 - -1.3 103,712 - -0.1 103,712 - -0.1 103,712 - +0.0 103,712 - -0.6 103,712 - +1.1 103,795 - GFF4462 0.14 -1.1 104,025 + GFF4462 0.56 -1.3 104,241 + +0.3 104,241 + +0.2 104,279 + +0.3 104,279 + +0.2 104,279 + -0.5 104,280 - +0.0 104,280 - -0.1 104,280 - +0.3 104,280 - +0.3 104,660 + GFF4463 0.31 -1.3 104,660 + GFF4463 0.31 +0.2 104,660 + GFF4463 0.31 -0.4 104,661 - GFF4463 0.31 -1.5 104,661 - GFF4463 0.31 -1.1 104,828 + GFF4463 0.44 -0.5 104,828 + GFF4463 0.44 +0.3 104,828 + GFF4463 0.44 -1.2 104,828 + GFF4463 0.44 -1.3 104,829 - GFF4463 0.44 -0.3 104,829 - GFF4463 0.44 -0.3 104,829 - GFF4463 0.44 -0.1 105,062 + GFF4463 0.63 +1.0 105,206 + GFF4463 0.74 -0.4 105,206 + GFF4463 0.74 -1.7 105,207 - GFF4463 0.74 -0.5 105,207 - GFF4463 0.74 -0.1 105,326 + GFF4463 0.84 -1.2 105,326 + GFF4463 0.84 -1.9 105,327 - GFF4463 0.84 -0.1 105,327 - GFF4463 0.84 +0.9 105,327 - GFF4463 0.84 +2.3 105,903 - GFF4465 0.20 -0.4 105,903 - GFF4465 0.20 +0.2 105,903 - GFF4465 0.20 -0.4 105,903 - GFF4465 0.20 +1.6 106,175 - GFF4465 0.49 +0.0 106,453 + GFF4465 0.78 -0.8 106,453 + GFF4465 0.78 +0.8
Or see this region's nucleotide sequence