Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4456 and GFF4457 overlap by 4 nucleotides GFF4457 and GFF4458 overlap by 35 nucleotides GFF4458 and GFF4459 are separated by 21 nucleotides GFF4459 and GFF4460 are separated by 15 nucleotides
GFF4456 - Shikimate 5-dehydrogenase I alpha (EC 1.1.1.25), at 97,598 to 98,470
GFF4456
GFF4457 - Monofunctional biosynthetic peptidoglycan transglycosylase, at 98,467 to 99,210
GFF4457
GFF4458 - hypothetical protein, at 99,176 to 99,580
GFF4458
GFF4459 - Acyl-CoA:1-acyl-sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51), at 99,602 to 100,465
GFF4459
GFF4460 - Phosphatidate cytidylyltransferase (EC 2.7.7.41), at 100,481 to 101,470
GFF4460
Position (kb) 99
100 Strain fitness (log2 ratio) -2
-1
0
1 at 98.276 kb on + strand, within GFF4456 at 98.276 kb on + strand, within GFF4456 at 98.276 kb on + strand, within GFF4456 at 98.276 kb on + strand, within GFF4456 at 98.276 kb on + strand, within GFF4456 at 98.276 kb on + strand, within GFF4456 at 98.276 kb on + strand, within GFF4456 at 98.276 kb on + strand, within GFF4456 at 98.277 kb on - strand, within GFF4456 at 98.277 kb on - strand, within GFF4456 at 98.277 kb on - strand, within GFF4456 at 98.277 kb on - strand, within GFF4456 at 98.656 kb on + strand, within GFF4457 at 98.656 kb on + strand, within GFF4457 at 98.990 kb on - strand, within GFF4457 at 99.046 kb on + strand, within GFF4457 at 99.046 kb on + strand, within GFF4457 at 99.046 kb on + strand, within GFF4457 at 99.047 kb on - strand, within GFF4457 at 99.049 kb on + strand, within GFF4457 at 99.050 kb on - strand, within GFF4457 at 99.120 kb on + strand, within GFF4457 at 99.151 kb on + strand at 99.261 kb on + strand, within GFF4458 at 99.261 kb on + strand, within GFF4458 at 99.261 kb on + strand, within GFF4458 at 99.262 kb on - strand, within GFF4458 at 99.262 kb on - strand, within GFF4458 at 99.351 kb on + strand, within GFF4458 at 99.351 kb on + strand, within GFF4458 at 99.351 kb on + strand, within GFF4458 at 99.352 kb on - strand, within GFF4458 at 99.352 kb on - strand, within GFF4458 at 99.441 kb on + strand, within GFF4458 at 99.441 kb on + strand, within GFF4458 at 99.441 kb on + strand, within GFF4458 at 99.442 kb on - strand, within GFF4458 at 99.442 kb on - strand, within GFF4458 at 99.442 kb on - strand, within GFF4458 at 99.442 kb on - strand, within GFF4458 at 99.442 kb on - strand, within GFF4458 at 99.442 kb on - strand, within GFF4458 at 99.442 kb on - strand, within GFF4458 at 99.442 kb on - strand, within GFF4458 at 99.587 kb on + strand at 99.588 kb on - strand at 99.601 kb on + strand at 99.601 kb on + strand at 99.601 kb on + strand at 99.602 kb on - strand at 99.602 kb on - strand at 99.602 kb on - strand at 99.674 kb on + strand at 99.674 kb on + strand at 99.674 kb on + strand at 99.675 kb on - strand at 99.675 kb on - strand at 99.707 kb on + strand, within GFF4459 at 99.707 kb on + strand, within GFF4459 at 99.708 kb on - strand, within GFF4459 at 99.708 kb on - strand, within GFF4459 at 99.708 kb on - strand, within GFF4459 at 99.708 kb on - strand, within GFF4459 at 99.863 kb on + strand, within GFF4459 at 100.256 kb on + strand, within GFF4459 at 100.284 kb on + strand, within GFF4459 at 100.284 kb on + strand, within GFF4459 at 100.284 kb on + strand, within GFF4459 at 100.285 kb on - strand, within GFF4459 at 100.285 kb on - strand, within GFF4459
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 98,276 + GFF4456 0.78 +0.3 98,276 + GFF4456 0.78 +0.7 98,276 + GFF4456 0.78 -1.6 98,276 + GFF4456 0.78 -2.7 98,276 + GFF4456 0.78 -1.0 98,276 + GFF4456 0.78 -1.5 98,276 + GFF4456 0.78 -0.2 98,276 + GFF4456 0.78 -0.2 98,277 - GFF4456 0.78 -0.9 98,277 - GFF4456 0.78 -2.3 98,277 - GFF4456 0.78 +0.7 98,277 - GFF4456 0.78 -0.3 98,656 + GFF4457 0.25 +0.1 98,656 + GFF4457 0.25 -1.8 98,990 - GFF4457 0.70 +0.6 99,046 + GFF4457 0.78 -0.7 99,046 + GFF4457 0.78 -0.9 99,046 + GFF4457 0.78 -0.1 99,047 - GFF4457 0.78 -1.2 99,049 + GFF4457 0.78 -0.8 99,050 - GFF4457 0.78 -0.2 99,120 + GFF4457 0.88 -0.5 99,151 + +0.3 99,261 + GFF4458 0.21 -1.8 99,261 + GFF4458 0.21 +0.7 99,261 + GFF4458 0.21 -0.1 99,262 - GFF4458 0.21 -0.1 99,262 - GFF4458 0.21 +0.5 99,351 + GFF4458 0.43 -1.3 99,351 + GFF4458 0.43 +1.1 99,351 + GFF4458 0.43 -0.7 99,352 - GFF4458 0.43 -0.2 99,352 - GFF4458 0.43 +0.6 99,441 + GFF4458 0.65 -0.1 99,441 + GFF4458 0.65 +1.4 99,441 + GFF4458 0.65 -0.7 99,442 - GFF4458 0.66 +0.9 99,442 - GFF4458 0.66 +0.4 99,442 - GFF4458 0.66 -0.4 99,442 - GFF4458 0.66 -0.1 99,442 - GFF4458 0.66 +0.2 99,442 - GFF4458 0.66 -0.5 99,442 - GFF4458 0.66 -1.3 99,442 - GFF4458 0.66 +0.2 99,587 + +0.5 99,588 - -0.1 99,601 + +1.2 99,601 + -1.8 99,601 + +0.1 99,602 - -1.0 99,602 - -0.7 99,602 - -0.2 99,674 + -0.6 99,674 + +1.1 99,674 + -0.4 99,675 - -0.3 99,675 - +0.0 99,707 + GFF4459 0.12 +0.6 99,707 + GFF4459 0.12 +0.3 99,708 - GFF4459 0.12 -0.3 99,708 - GFF4459 0.12 -0.9 99,708 - GFF4459 0.12 -0.4 99,708 - GFF4459 0.12 -1.1 99,863 + GFF4459 0.30 -1.4 100,256 + GFF4459 0.76 +0.3 100,284 + GFF4459 0.79 +0.3 100,284 + GFF4459 0.79 +0.4 100,284 + GFF4459 0.79 -0.1 100,285 - GFF4459 0.79 -1.3 100,285 - GFF4459 0.79 -0.2
Or see this region's nucleotide sequence