Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4200 and GFF4201 are separated by 3 nucleotides GFF4201 and GFF4202 overlap by 4 nucleotides GFF4202 and GFF4203 are separated by 1 nucleotides
GFF4200 - Acyl-CoA dehydrogenase, long-chain specific (EC 1.3.8.8), at 115,338 to 116,498
GFF4200
GFF4201 - Acyl-CoA dehydrogenase, at 116,502 to 117,614
GFF4201
GFF4202 - Enoyl-CoA hydratase (EC 4.2.1.17), at 117,611 to 118,372
GFF4202
GFF4203 - Acetyl-CoA synthetase (ADP-forming) alpha and beta chains, putative, at 118,374 to 120,509
GFF4203
Position (kb)
116
117
118 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 115.833 kb on + strand, within GFF4200 at 115.833 kb on + strand, within GFF4200 at 115.833 kb on + strand, within GFF4200 at 115.833 kb on + strand, within GFF4200 at 115.833 kb on + strand, within GFF4200 at 115.834 kb on - strand, within GFF4200 at 115.834 kb on - strand, within GFF4200 at 115.834 kb on - strand, within GFF4200 at 115.834 kb on - strand, within GFF4200 at 115.834 kb on - strand, within GFF4200 at 115.847 kb on + strand, within GFF4200 at 115.847 kb on + strand, within GFF4200 at 116.412 kb on + strand at 116.412 kb on + strand at 116.412 kb on + strand at 116.412 kb on + strand at 116.413 kb on - strand at 116.413 kb on - strand at 116.413 kb on - strand at 116.413 kb on - strand at 116.413 kb on - strand at 116.413 kb on - strand at 116.415 kb on - strand at 116.946 kb on + strand, within GFF4201 at 116.947 kb on - strand, within GFF4201 at 117.291 kb on + strand, within GFF4201 at 117.291 kb on + strand, within GFF4201 at 117.291 kb on + strand, within GFF4201 at 117.291 kb on + strand, within GFF4201 at 117.291 kb on + strand, within GFF4201 at 117.292 kb on - strand, within GFF4201 at 118.109 kb on + strand, within GFF4202 at 118.109 kb on + strand, within GFF4202 at 118.110 kb on - strand, within GFF4202 at 118.358 kb on + strand at 118.358 kb on + strand at 118.358 kb on + strand at 118.497 kb on + strand at 118.498 kb on - strand at 118.498 kb on - strand at 118.498 kb on - strand at 118.503 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 115,833 + GFF4200 0.43 +0.1 115,833 + GFF4200 0.43 +0.6 115,833 + GFF4200 0.43 -0.8 115,833 + GFF4200 0.43 +0.3 115,833 + GFF4200 0.43 -2.1 115,834 - GFF4200 0.43 -0.4 115,834 - GFF4200 0.43 -1.0 115,834 - GFF4200 0.43 +0.4 115,834 - GFF4200 0.43 +0.2 115,834 - GFF4200 0.43 +0.7 115,847 + GFF4200 0.44 -1.6 115,847 + GFF4200 0.44 +0.4 116,412 + -0.5 116,412 + -1.6 116,412 + +0.4 116,412 + -0.9 116,413 - -0.3 116,413 - +1.1 116,413 - -0.4 116,413 - -0.9 116,413 - -0.9 116,413 - +0.5 116,415 - -0.5 116,946 + GFF4201 0.40 -1.1 116,947 - GFF4201 0.40 +0.8 117,291 + GFF4201 0.71 -2.8 117,291 + GFF4201 0.71 -1.2 117,291 + GFF4201 0.71 -0.5 117,291 + GFF4201 0.71 -0.5 117,291 + GFF4201 0.71 +0.4 117,292 - GFF4201 0.71 -0.3 118,109 + GFF4202 0.65 -2.6 118,109 + GFF4202 0.65 +0.0 118,110 - GFF4202 0.65 +0.9 118,358 + -0.4 118,358 + +0.1 118,358 + +0.0 118,497 + -0.4 118,498 - -2.2 118,498 - +0.0 118,498 - -0.2 118,503 + -0.2
Or see this region's nucleotide sequence