Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4149 and GFF4150 are separated by 295 nucleotides GFF4150 and GFF4151 are separated by 68 nucleotides GFF4151 and GFF4152 are separated by 2 nucleotides
GFF4149 - hypothetical protein, at 55,190 to 56,152
GFF4149
GFF4150 - Salicylate hydroxylase (EC 1.14.13.1), at 56,448 to 57,704
GFF4150
GFF4151 - RidA/YER057c/UK114 superfamily, group 1, at 57,773 to 58,405
GFF4151
GFF4152 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 58,408 to 59,574
GFF4152
Position (kb) 56
57
58 Strain fitness (log2 ratio) -2
-1
0
1
2 at 56.133 kb on + strand at 56.201 kb on - strand at 56.282 kb on + strand at 56.282 kb on + strand at 56.282 kb on + strand at 56.283 kb on - strand at 56.283 kb on - strand at 56.335 kb on - strand at 56.667 kb on + strand, within GFF4150 at 56.667 kb on + strand, within GFF4150 at 56.668 kb on - strand, within GFF4150 at 56.668 kb on - strand, within GFF4150 at 56.736 kb on + strand, within GFF4150 at 56.736 kb on + strand, within GFF4150 at 56.963 kb on - strand, within GFF4150 at 57.424 kb on - strand, within GFF4150 at 57.424 kb on - strand, within GFF4150 at 57.471 kb on + strand, within GFF4150 at 57.684 kb on + strand at 57.684 kb on + strand at 57.814 kb on - strand at 57.962 kb on + strand, within GFF4151 at 57.963 kb on - strand, within GFF4151 at 57.963 kb on - strand, within GFF4151 at 57.995 kb on + strand, within GFF4151 at 57.995 kb on + strand, within GFF4151 at 58.166 kb on + strand, within GFF4151 at 58.167 kb on - strand, within GFF4151 at 58.167 kb on - strand, within GFF4151 at 58.184 kb on + strand, within GFF4151 at 58.185 kb on - strand, within GFF4151 at 58.185 kb on - strand, within GFF4151 at 58.241 kb on + strand, within GFF4151 at 58.645 kb on + strand, within GFF4152
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 56,133 + +0.2 56,201 - -0.4 56,282 + -0.5 56,282 + +0.7 56,282 + +1.1 56,283 - -0.9 56,283 - -0.6 56,335 - +1.8 56,667 + GFF4150 0.17 -1.2 56,667 + GFF4150 0.17 -1.4 56,668 - GFF4150 0.18 -1.5 56,668 - GFF4150 0.18 -0.0 56,736 + GFF4150 0.23 -0.8 56,736 + GFF4150 0.23 -0.5 56,963 - GFF4150 0.41 -0.8 57,424 - GFF4150 0.78 +0.5 57,424 - GFF4150 0.78 -1.0 57,471 + GFF4150 0.81 -0.6 57,684 + +0.2 57,684 + -2.0 57,814 - -0.3 57,962 + GFF4151 0.30 -1.4 57,963 - GFF4151 0.30 -0.9 57,963 - GFF4151 0.30 -0.5 57,995 + GFF4151 0.35 +0.8 57,995 + GFF4151 0.35 +0.1 58,166 + GFF4151 0.62 +0.2 58,167 - GFF4151 0.62 +1.4 58,167 - GFF4151 0.62 -0.5 58,184 + GFF4151 0.65 +1.4 58,185 - GFF4151 0.65 -0.1 58,185 - GFF4151 0.65 -0.5 58,241 + GFF4151 0.74 +1.8 58,645 + GFF4152 0.20 +0.4
Or see this region's nucleotide sequence