Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3975 and GFF3976 overlap by 4 nucleotides GFF3976 and GFF3977 are separated by 49 nucleotides GFF3977 and GFF3978 are separated by 1 nucleotides
GFF3975 - Metal-dependent hydrolase, at 178,587 to 179,399
GFF3975
GFF3976 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 179,396 to 180,130
GFF3976
GFF3977 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 180,180 to 181,346
GFF3977
GFF3978 - ABC-type branched-chain amino acid transport systems, periplasmic component, at 181,348 to 182,493
GFF3978
Position (kb) 180
181
182 Strain fitness (log2 ratio) -2
-1
0
1 at 179.212 kb on - strand, within GFF3975 at 179.212 kb on - strand, within GFF3975 at 179.230 kb on - strand, within GFF3975 at 179.241 kb on + strand, within GFF3975 at 179.241 kb on + strand, within GFF3975 at 179.242 kb on - strand, within GFF3975 at 179.242 kb on - strand, within GFF3975 at 179.242 kb on - strand, within GFF3975 at 179.244 kb on + strand, within GFF3975 at 179.244 kb on + strand, within GFF3975 at 179.245 kb on - strand, within GFF3975 at 179.245 kb on - strand, within GFF3975 at 179.245 kb on - strand, within GFF3975 at 179.245 kb on - strand, within GFF3975 at 179.825 kb on + strand, within GFF3976 at 179.825 kb on + strand, within GFF3976 at 179.825 kb on + strand, within GFF3976 at 179.863 kb on + strand, within GFF3976 at 179.943 kb on - strand, within GFF3976 at 180.089 kb on + strand at 180.090 kb on - strand at 180.090 kb on - strand at 180.090 kb on - strand at 180.090 kb on - strand at 180.325 kb on - strand, within GFF3977 at 180.354 kb on + strand, within GFF3977 at 180.355 kb on - strand, within GFF3977 at 180.708 kb on + strand, within GFF3977 at 180.898 kb on - strand, within GFF3977 at 180.913 kb on - strand, within GFF3977 at 180.913 kb on - strand, within GFF3977 at 181.111 kb on - strand, within GFF3977 at 181.111 kb on - strand, within GFF3977 at 181.134 kb on + strand, within GFF3977 at 181.255 kb on - strand at 181.859 kb on + strand, within GFF3978 at 181.859 kb on + strand, within GFF3978 at 181.860 kb on - strand, within GFF3978 at 181.860 kb on - strand, within GFF3978 at 181.864 kb on + strand, within GFF3978 at 182.029 kb on + strand, within GFF3978 at 182.030 kb on - strand, within GFF3978 at 182.030 kb on - strand, within GFF3978 at 182.053 kb on + strand, within GFF3978 at 182.054 kb on - strand, within GFF3978 at 182.069 kb on - strand, within GFF3978 at 182.272 kb on + strand, within GFF3978 at 182.272 kb on + strand, within GFF3978 at 182.273 kb on - strand, within GFF3978 at 182.281 kb on + strand, within GFF3978
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 179,212 - GFF3975 0.77 -0.9 179,212 - GFF3975 0.77 -0.8 179,230 - GFF3975 0.79 +1.0 179,241 + GFF3975 0.80 +1.4 179,241 + GFF3975 0.80 +0.3 179,242 - GFF3975 0.81 -0.6 179,242 - GFF3975 0.81 +0.2 179,242 - GFF3975 0.81 -0.4 179,244 + GFF3975 0.81 -0.2 179,244 + GFF3975 0.81 -1.2 179,245 - GFF3975 0.81 -0.5 179,245 - GFF3975 0.81 -0.0 179,245 - GFF3975 0.81 -0.5 179,245 - GFF3975 0.81 +0.8 179,825 + GFF3976 0.58 +0.2 179,825 + GFF3976 0.58 -1.0 179,825 + GFF3976 0.58 -0.6 179,863 + GFF3976 0.64 -0.1 179,943 - GFF3976 0.74 -0.7 180,089 + -0.7 180,090 - -0.4 180,090 - +1.2 180,090 - -0.8 180,090 - -0.2 180,325 - GFF3977 0.12 +0.0 180,354 + GFF3977 0.15 -0.4 180,355 - GFF3977 0.15 -0.1 180,708 + GFF3977 0.45 -1.2 180,898 - GFF3977 0.62 +0.2 180,913 - GFF3977 0.63 -1.2 180,913 - GFF3977 0.63 -0.2 181,111 - GFF3977 0.80 +0.7 181,111 - GFF3977 0.80 +0.7 181,134 + GFF3977 0.82 -0.2 181,255 - +0.2 181,859 + GFF3978 0.45 +0.3 181,859 + GFF3978 0.45 +0.8 181,860 - GFF3978 0.45 -0.8 181,860 - GFF3978 0.45 -0.1 181,864 + GFF3978 0.45 +0.9 182,029 + GFF3978 0.59 -1.0 182,030 - GFF3978 0.60 -0.8 182,030 - GFF3978 0.60 -0.4 182,053 + GFF3978 0.62 +0.2 182,054 - GFF3978 0.62 -2.2 182,069 - GFF3978 0.63 -1.2 182,272 + GFF3978 0.81 +0.7 182,272 + GFF3978 0.81 -0.9 182,273 - GFF3978 0.81 +0.5 182,281 + GFF3978 0.81 -0.8
Or see this region's nucleotide sequence