Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3915 and GFF3916 are separated by 147 nucleotides GFF3916 and GFF3917 overlap by 8 nucleotides GFF3917 and GFF3918 are separated by 29 nucleotides GFF3918 and GFF3919 are separated by 4 nucleotides
GFF3915 - Ferrous iron transport periplasmic protein EfeO, contains peptidase-M75 domain and (frequently) cupredoxin-like domain, at 121,176 to 122,012
GFF3915
GFF3916 - Ferrous iron transporter-associated protein FeoA, at 122,160 to 122,459
GFF3916
GFF3917 - Ferrous iron transporter FeoB, at 122,452 to 124,347
GFF3917
GFF3918 - hypothetical protein, at 124,377 to 124,679
GFF3918
GFF3919 - Putative n-hydroxybenzoate hydroxylase, at 124,684 to 125,868
GFF3919
Position (kb) 122
123
124
125 Strain fitness (log2 ratio) -2
-1
0
1
2 at 121.542 kb on + strand, within GFF3915 at 121.543 kb on - strand, within GFF3915 at 121.729 kb on - strand, within GFF3915 at 121.729 kb on - strand, within GFF3915 at 121.729 kb on - strand, within GFF3915 at 121.776 kb on + strand, within GFF3915 at 121.776 kb on + strand, within GFF3915 at 121.777 kb on - strand, within GFF3915 at 121.777 kb on - strand, within GFF3915 at 121.777 kb on - strand, within GFF3915 at 121.881 kb on + strand, within GFF3915 at 121.881 kb on + strand, within GFF3915 at 121.881 kb on + strand, within GFF3915 at 121.881 kb on + strand, within GFF3915 at 121.881 kb on + strand, within GFF3915 at 121.881 kb on + strand, within GFF3915 at 121.881 kb on + strand, within GFF3915 at 121.881 kb on + strand, within GFF3915 at 121.881 kb on + strand, within GFF3915 at 121.882 kb on - strand, within GFF3915 at 121.882 kb on - strand, within GFF3915 at 121.882 kb on - strand, within GFF3915 at 121.882 kb on - strand, within GFF3915 at 121.882 kb on - strand, within GFF3915 at 121.882 kb on - strand, within GFF3915 at 121.882 kb on - strand, within GFF3915 at 121.882 kb on - strand, within GFF3915 at 121.911 kb on + strand, within GFF3915 at 121.911 kb on + strand, within GFF3915 at 121.912 kb on - strand, within GFF3915 at 121.912 kb on - strand, within GFF3915 at 121.912 kb on - strand, within GFF3915 at 121.912 kb on - strand, within GFF3915 at 121.912 kb on - strand, within GFF3915 at 122.010 kb on + strand at 122.010 kb on + strand at 122.011 kb on - strand at 122.011 kb on - strand at 122.124 kb on + strand at 122.124 kb on + strand at 122.124 kb on + strand at 122.124 kb on + strand at 122.124 kb on + strand at 122.124 kb on + strand at 122.124 kb on + strand at 122.124 kb on + strand at 122.124 kb on + strand at 122.124 kb on + strand at 122.124 kb on + strand at 122.124 kb on + strand at 122.124 kb on + strand at 122.124 kb on + strand at 122.125 kb on - strand at 122.125 kb on - strand at 122.125 kb on - strand at 122.125 kb on - strand at 122.125 kb on - strand at 122.125 kb on - strand at 122.125 kb on - strand at 122.125 kb on - strand at 122.125 kb on - strand at 122.125 kb on - strand at 122.125 kb on - strand at 122.125 kb on - strand at 122.125 kb on - strand at 122.125 kb on - strand at 122.125 kb on - strand at 122.125 kb on - strand at 122.125 kb on - strand at 122.125 kb on - strand at 122.125 kb on - strand at 122.125 kb on - strand at 122.593 kb on + strand at 122.594 kb on - strand at 122.594 kb on - strand at 122.594 kb on - strand at 122.680 kb on + strand, within GFF3917 at 122.680 kb on + strand, within GFF3917 at 122.681 kb on - strand, within GFF3917 at 122.681 kb on - strand, within GFF3917 at 122.742 kb on + strand, within GFF3917 at 122.742 kb on + strand, within GFF3917 at 122.742 kb on + strand, within GFF3917 at 122.742 kb on + strand, within GFF3917 at 122.743 kb on - strand, within GFF3917 at 122.743 kb on - strand, within GFF3917 at 122.743 kb on - strand, within GFF3917 at 122.743 kb on - strand, within GFF3917 at 123.409 kb on + strand, within GFF3917 at 123.409 kb on + strand, within GFF3917 at 123.610 kb on + strand, within GFF3917 at 123.610 kb on + strand, within GFF3917 at 123.610 kb on + strand, within GFF3917 at 123.610 kb on + strand, within GFF3917 at 123.610 kb on + strand, within GFF3917 at 123.610 kb on + strand, within GFF3917 at 123.610 kb on + strand, within GFF3917 at 123.610 kb on + strand, within GFF3917 at 123.611 kb on - strand, within GFF3917 at 123.611 kb on - strand, within GFF3917 at 123.611 kb on - strand, within GFF3917 at 123.611 kb on - strand, within GFF3917 at 123.611 kb on - strand, within GFF3917 at 123.611 kb on - strand, within GFF3917 at 123.611 kb on - strand, within GFF3917 at 123.611 kb on - strand, within GFF3917 at 123.694 kb on + strand, within GFF3917 at 123.694 kb on + strand, within GFF3917 at 123.694 kb on + strand, within GFF3917 at 123.694 kb on + strand, within GFF3917 at 123.694 kb on + strand, within GFF3917 at 123.694 kb on + strand, within GFF3917 at 123.695 kb on - strand, within GFF3917 at 123.695 kb on - strand, within GFF3917 at 123.695 kb on - strand, within GFF3917 at 123.695 kb on - strand, within GFF3917 at 123.695 kb on - strand, within GFF3917 at 123.695 kb on - strand, within GFF3917 at 123.695 kb on - strand, within GFF3917 at 123.695 kb on - strand, within GFF3917 at 123.695 kb on - strand, within GFF3917 at 123.695 kb on - strand, within GFF3917 at 123.695 kb on - strand, within GFF3917 at 123.695 kb on - strand, within GFF3917 at 123.794 kb on - strand, within GFF3917 at 123.832 kb on + strand, within GFF3917 at 123.832 kb on + strand, within GFF3917 at 123.832 kb on + strand, within GFF3917 at 123.833 kb on - strand, within GFF3917 at 123.833 kb on - strand, within GFF3917 at 123.833 kb on - strand, within GFF3917 at 123.955 kb on + strand, within GFF3917 at 123.956 kb on - strand, within GFF3917 at 124.090 kb on + strand, within GFF3917 at 124.090 kb on + strand, within GFF3917 at 124.090 kb on + strand, within GFF3917 at 124.090 kb on + strand, within GFF3917 at 124.090 kb on + strand, within GFF3917 at 124.090 kb on + strand, within GFF3917 at 124.090 kb on + strand, within GFF3917 at 124.090 kb on + strand, within GFF3917 at 124.090 kb on + strand, within GFF3917 at 124.090 kb on + strand, within GFF3917 at 124.090 kb on + strand, within GFF3917 at 124.090 kb on + strand, within GFF3917 at 124.090 kb on - strand, within GFF3917 at 124.091 kb on - strand, within GFF3917 at 124.091 kb on - strand, within GFF3917 at 124.091 kb on - strand, within GFF3917 at 124.091 kb on - strand, within GFF3917 at 124.091 kb on - strand, within GFF3917 at 124.091 kb on - strand, within GFF3917 at 124.091 kb on - strand, within GFF3917 at 124.091 kb on - strand, within GFF3917 at 124.091 kb on - strand, within GFF3917 at 124.091 kb on - strand, within GFF3917 at 124.091 kb on - strand, within GFF3917 at 124.091 kb on - strand, within GFF3917 at 124.189 kb on + strand at 124.189 kb on + strand at 124.189 kb on + strand at 124.189 kb on + strand at 124.189 kb on + strand at 124.189 kb on + strand at 124.189 kb on + strand at 124.189 kb on + strand at 124.190 kb on - strand at 124.190 kb on - strand at 124.190 kb on - strand at 124.190 kb on - strand at 124.211 kb on + strand at 124.255 kb on + strand at 124.255 kb on + strand at 124.256 kb on - strand at 124.277 kb on - strand at 124.277 kb on - strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.294 kb on + strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.295 kb on - strand at 124.296 kb on + strand at 124.296 kb on + strand at 124.297 kb on - strand at 124.297 kb on - strand at 124.297 kb on - strand at 124.297 kb on - strand at 124.297 kb on - strand at 124.297 kb on - strand at 124.297 kb on - strand at 124.315 kb on + strand at 124.315 kb on + strand at 124.315 kb on + strand at 124.315 kb on - strand at 124.316 kb on - strand at 124.316 kb on - strand at 124.345 kb on + strand at 124.345 kb on + strand at 124.346 kb on - strand at 124.366 kb on + strand at 124.366 kb on + strand at 124.367 kb on - strand at 124.367 kb on - strand at 124.367 kb on - strand at 124.425 kb on + strand, within GFF3918
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 121,542 + GFF3915 0.44 +0.8 121,543 - GFF3915 0.44 -0.1 121,729 - GFF3915 0.66 -1.5 121,729 - GFF3915 0.66 -0.4 121,729 - GFF3915 0.66 +0.4 121,776 + GFF3915 0.72 +0.3 121,776 + GFF3915 0.72 +0.1 121,777 - GFF3915 0.72 -0.0 121,777 - GFF3915 0.72 +0.7 121,777 - GFF3915 0.72 -0.5 121,881 + GFF3915 0.84 -0.3 121,881 + GFF3915 0.84 -0.0 121,881 + GFF3915 0.84 +0.8 121,881 + GFF3915 0.84 -0.8 121,881 + GFF3915 0.84 -0.2 121,881 + GFF3915 0.84 -0.1 121,881 + GFF3915 0.84 -0.8 121,881 + GFF3915 0.84 -1.3 121,881 + GFF3915 0.84 -0.7 121,882 - GFF3915 0.84 -0.7 121,882 - GFF3915 0.84 +0.2 121,882 - GFF3915 0.84 -0.2 121,882 - GFF3915 0.84 -2.0 121,882 - GFF3915 0.84 -1.1 121,882 - GFF3915 0.84 +0.0 121,882 - GFF3915 0.84 +0.5 121,882 - GFF3915 0.84 -0.0 121,911 + GFF3915 0.88 -0.1 121,911 + GFF3915 0.88 -0.7 121,912 - GFF3915 0.88 +2.0 121,912 - GFF3915 0.88 +0.1 121,912 - GFF3915 0.88 +0.9 121,912 - GFF3915 0.88 -1.5 121,912 - GFF3915 0.88 -0.0 122,010 + -0.4 122,010 + +1.0 122,011 - +0.6 122,011 - -0.7 122,124 + +0.1 122,124 + -1.1 122,124 + -0.2 122,124 + -0.5 122,124 + +0.1 122,124 + +0.2 122,124 + +0.3 122,124 + -0.3 122,124 + -1.0 122,124 + -1.0 122,124 + +0.5 122,124 + -0.1 122,124 + -0.2 122,124 + +2.2 122,125 - -0.9 122,125 - -1.1 122,125 - -0.1 122,125 - +0.9 122,125 - +0.2 122,125 - +1.4 122,125 - +0.7 122,125 - +0.0 122,125 - +1.4 122,125 - -0.3 122,125 - -1.7 122,125 - -1.2 122,125 - +0.3 122,125 - -0.3 122,125 - +0.5 122,125 - -1.3 122,125 - -0.5 122,125 - +0.9 122,125 - +0.9 122,125 - -0.0 122,593 + +0.7 122,594 - -0.4 122,594 - -1.0 122,594 - -1.3 122,680 + GFF3917 0.12 -0.3 122,680 + GFF3917 0.12 -0.4 122,681 - GFF3917 0.12 +0.7 122,681 - GFF3917 0.12 -0.9 122,742 + GFF3917 0.15 -0.0 122,742 + GFF3917 0.15 -0.2 122,742 + GFF3917 0.15 +0.3 122,742 + GFF3917 0.15 +0.1 122,743 - GFF3917 0.15 -0.5 122,743 - GFF3917 0.15 +0.2 122,743 - GFF3917 0.15 -1.4 122,743 - GFF3917 0.15 -0.5 123,409 + GFF3917 0.50 -0.4 123,409 + GFF3917 0.50 -0.3 123,610 + GFF3917 0.61 -0.9 123,610 + GFF3917 0.61 +0.6 123,610 + GFF3917 0.61 +0.7 123,610 + GFF3917 0.61 +0.3 123,610 + GFF3917 0.61 -0.2 123,610 + GFF3917 0.61 +0.1 123,610 + GFF3917 0.61 +0.6 123,610 + GFF3917 0.61 +0.3 123,611 - GFF3917 0.61 +0.9 123,611 - GFF3917 0.61 -0.1 123,611 - GFF3917 0.61 +0.5 123,611 - GFF3917 0.61 +0.0 123,611 - GFF3917 0.61 -0.2 123,611 - GFF3917 0.61 +0.0 123,611 - GFF3917 0.61 -0.5 123,611 - GFF3917 0.61 +0.5 123,694 + GFF3917 0.66 +0.2 123,694 + GFF3917 0.66 +0.2 123,694 + GFF3917 0.66 +0.2 123,694 + GFF3917 0.66 +0.7 123,694 + GFF3917 0.66 -0.1 123,694 + GFF3917 0.66 +0.7 123,695 - GFF3917 0.66 -1.1 123,695 - GFF3917 0.66 -0.3 123,695 - GFF3917 0.66 -0.5 123,695 - GFF3917 0.66 -0.3 123,695 - GFF3917 0.66 -0.1 123,695 - GFF3917 0.66 +0.3 123,695 - GFF3917 0.66 -0.1 123,695 - GFF3917 0.66 +0.0 123,695 - GFF3917 0.66 -0.5 123,695 - GFF3917 0.66 -1.0 123,695 - GFF3917 0.66 +0.6 123,695 - GFF3917 0.66 -0.2 123,794 - GFF3917 0.71 +0.5 123,832 + GFF3917 0.73 -1.1 123,832 + GFF3917 0.73 -0.2 123,832 + GFF3917 0.73 -0.1 123,833 - GFF3917 0.73 -1.9 123,833 - GFF3917 0.73 -0.5 123,833 - GFF3917 0.73 +1.1 123,955 + GFF3917 0.79 -0.4 123,956 - GFF3917 0.79 +1.1 124,090 + GFF3917 0.86 -0.3 124,090 + GFF3917 0.86 -1.6 124,090 + GFF3917 0.86 +0.4 124,090 + GFF3917 0.86 -1.2 124,090 + GFF3917 0.86 +1.8 124,090 + GFF3917 0.86 +1.3 124,090 + GFF3917 0.86 +0.6 124,090 + GFF3917 0.86 +0.1 124,090 + GFF3917 0.86 +1.1 124,090 + GFF3917 0.86 -0.2 124,090 + GFF3917 0.86 +0.0 124,090 + GFF3917 0.86 -0.1 124,090 - GFF3917 0.86 -1.0 124,091 - GFF3917 0.86 +1.8 124,091 - GFF3917 0.86 +1.4 124,091 - GFF3917 0.86 -0.5 124,091 - GFF3917 0.86 -0.4 124,091 - GFF3917 0.86 +0.1 124,091 - GFF3917 0.86 +0.0 124,091 - GFF3917 0.86 +0.6 124,091 - GFF3917 0.86 -0.0 124,091 - GFF3917 0.86 -1.0 124,091 - GFF3917 0.86 -1.0 124,091 - GFF3917 0.86 -1.4 124,091 - GFF3917 0.86 +2.1 124,189 + -0.3 124,189 + -0.9 124,189 + -0.3 124,189 + -1.6 124,189 + +0.6 124,189 + -0.6 124,189 + -0.2 124,189 + -0.7 124,190 - -0.3 124,190 - -1.7 124,190 - -0.2 124,190 - +0.4 124,211 + +0.8 124,255 + -0.2 124,255 + -0.4 124,256 - -1.1 124,277 - -1.0 124,277 - +0.2 124,294 + -0.3 124,294 + -0.3 124,294 + +1.6 124,294 + +0.2 124,294 + -0.8 124,294 + -0.4 124,294 + -0.7 124,294 + +0.2 124,294 + -0.5 124,294 + -0.5 124,294 + -0.7 124,294 + +0.6 124,294 + +0.8 124,294 + -2.6 124,294 + -1.4 124,294 + -0.1 124,294 + +0.4 124,294 + +1.4 124,294 + +0.5 124,294 + +0.4 124,294 + +0.1 124,294 + -1.3 124,294 + +0.3 124,294 + +0.5 124,295 - +0.3 124,295 - -0.4 124,295 - +1.2 124,295 - -1.0 124,295 - -0.6 124,295 - +0.8 124,295 - -0.0 124,295 - +0.2 124,295 - +0.0 124,295 - -0.4 124,295 - +0.1 124,295 - -0.2 124,295 - -0.5 124,295 - +0.5 124,295 - +1.8 124,295 - +0.1 124,295 - +0.3 124,295 - +0.2 124,295 - -0.8 124,295 - +0.6 124,295 - +0.1 124,295 - -0.8 124,295 - +0.5 124,295 - +0.2 124,295 - -0.8 124,295 - +0.3 124,295 - -0.5 124,296 + -1.5 124,296 + +0.1 124,297 - +0.0 124,297 - +1.2 124,297 - -0.7 124,297 - -0.6 124,297 - -0.4 124,297 - +1.0 124,297 - +0.1 124,315 + +0.9 124,315 + +0.4 124,315 + -1.4 124,315 - +0.8 124,316 - +0.8 124,316 - +1.0 124,345 + -1.9 124,345 + -0.9 124,346 - +0.8 124,366 + -1.3 124,366 + -0.5 124,367 - +2.4 124,367 - +0.8 124,367 - +0.3 124,425 + GFF3918 0.16 -0.1
Or see this region's nucleotide sequence