Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF37 and GFF38 are separated by 12 nucleotides GFF38 and GFF39 are separated by 44 nucleotides GFF39 and GFF40 are separated by 76 nucleotides
GFF37 - Similar to Agmatine deiminase, at 40,242 to 41,195
GFF37
GFF38 - no description, at 41,208 to 41,465
GFF38
GFF39 - Transcriptional regulator, LysR family, at 41,510 to 42,370
GFF39
GFF40 - no description, at 42,447 to 43,592
GFF40
Position (kb) 41
42
43 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 40.630 kb on + strand, within GFF37 at 40.889 kb on + strand, within GFF37 at 40.890 kb on - strand, within GFF37 at 41.102 kb on - strand at 41.102 kb on - strand at 41.169 kb on + strand at 41.420 kb on + strand, within GFF38 at 41.420 kb on + strand, within GFF38 at 41.420 kb on + strand, within GFF38 at 41.421 kb on - strand, within GFF38 at 41.421 kb on - strand, within GFF38 at 41.423 kb on + strand, within GFF38 at 41.423 kb on + strand, within GFF38 at 41.423 kb on + strand, within GFF38 at 41.424 kb on - strand, within GFF38 at 41.424 kb on - strand, within GFF38 at 41.424 kb on - strand, within GFF38 at 41.424 kb on - strand, within GFF38 at 41.526 kb on + strand at 41.526 kb on + strand at 41.526 kb on + strand at 41.526 kb on + strand at 41.527 kb on - strand at 41.527 kb on - strand at 41.527 kb on - strand at 41.623 kb on - strand, within GFF39 at 41.739 kb on + strand, within GFF39 at 41.740 kb on - strand, within GFF39 at 41.740 kb on - strand, within GFF39 at 41.961 kb on + strand, within GFF39 at 41.961 kb on + strand, within GFF39 at 41.961 kb on + strand, within GFF39 at 41.962 kb on - strand, within GFF39 at 42.096 kb on + strand, within GFF39 at 42.096 kb on + strand, within GFF39 at 42.096 kb on + strand, within GFF39 at 42.096 kb on + strand, within GFF39 at 42.097 kb on - strand, within GFF39 at 42.097 kb on - strand, within GFF39 at 42.097 kb on - strand, within GFF39 at 42.097 kb on - strand, within GFF39 at 42.102 kb on + strand, within GFF39 at 42.297 kb on + strand at 42.297 kb on + strand at 42.297 kb on + strand at 42.298 kb on - strand at 42.298 kb on - strand at 42.408 kb on + strand at 42.408 kb on + strand at 42.408 kb on + strand at 42.408 kb on + strand at 42.408 kb on + strand at 42.409 kb on - strand at 42.409 kb on - strand at 42.409 kb on - strand at 42.409 kb on - strand at 42.409 kb on - strand at 42.640 kb on + strand, within GFF40 at 42.641 kb on - strand, within GFF40 at 42.715 kb on + strand, within GFF40 at 42.715 kb on + strand, within GFF40 at 42.715 kb on + strand, within GFF40 at 42.715 kb on + strand, within GFF40 at 42.715 kb on + strand, within GFF40 at 42.715 kb on + strand, within GFF40 at 42.716 kb on - strand, within GFF40 at 42.716 kb on - strand, within GFF40 at 42.716 kb on - strand, within GFF40 at 42.716 kb on - strand, within GFF40 at 42.716 kb on - strand, within GFF40 at 42.716 kb on - strand, within GFF40 at 42.716 kb on - strand, within GFF40 at 42.716 kb on - strand, within GFF40 at 42.721 kb on + strand, within GFF40 at 42.751 kb on - strand, within GFF40 at 42.784 kb on + strand, within GFF40 at 42.784 kb on + strand, within GFF40 at 42.970 kb on + strand, within GFF40 at 43.039 kb on + strand, within GFF40 at 43.039 kb on + strand, within GFF40 at 43.039 kb on + strand, within GFF40 at 43.039 kb on + strand, within GFF40 at 43.039 kb on + strand, within GFF40 at 43.039 kb on + strand, within GFF40 at 43.039 kb on + strand, within GFF40 at 43.039 kb on + strand, within GFF40 at 43.040 kb on - strand, within GFF40 at 43.040 kb on - strand, within GFF40 at 43.165 kb on + strand, within GFF40 at 43.166 kb on - strand, within GFF40 at 43.343 kb on - strand, within GFF40 at 43.343 kb on - strand, within GFF40
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 40,630 + GFF37 0.41 -0.6 40,889 + GFF37 0.68 +0.0 40,890 - GFF37 0.68 -0.3 41,102 - -1.5 41,102 - +0.4 41,169 + +1.2 41,420 + GFF38 0.82 -1.1 41,420 + GFF38 0.82 +0.0 41,420 + GFF38 0.82 +0.0 41,421 - GFF38 0.83 -1.7 41,421 - GFF38 0.83 +3.0 41,423 + GFF38 0.83 -0.1 41,423 + GFF38 0.83 +0.0 41,423 + GFF38 0.83 -0.7 41,424 - GFF38 0.84 -0.2 41,424 - GFF38 0.84 -0.1 41,424 - GFF38 0.84 -1.0 41,424 - GFF38 0.84 +0.5 41,526 + +0.3 41,526 + -2.9 41,526 + +0.0 41,526 + -0.6 41,527 - -0.5 41,527 - +0.1 41,527 - -0.7 41,623 - GFF39 0.13 -1.5 41,739 + GFF39 0.27 +0.3 41,740 - GFF39 0.27 -2.1 41,740 - GFF39 0.27 -0.3 41,961 + GFF39 0.52 +0.3 41,961 + GFF39 0.52 -1.4 41,961 + GFF39 0.52 -0.1 41,962 - GFF39 0.52 -0.8 42,096 + GFF39 0.68 -0.9 42,096 + GFF39 0.68 -1.5 42,096 + GFF39 0.68 +0.0 42,096 + GFF39 0.68 +1.0 42,097 - GFF39 0.68 -0.5 42,097 - GFF39 0.68 -0.6 42,097 - GFF39 0.68 -1.7 42,097 - GFF39 0.68 -0.5 42,102 + GFF39 0.69 -2.6 42,297 + -0.2 42,297 + +1.5 42,297 + +1.0 42,298 - -1.2 42,298 - +0.6 42,408 + +0.5 42,408 + -0.4 42,408 + -0.2 42,408 + +0.5 42,408 + -1.4 42,409 - -1.0 42,409 - -1.1 42,409 - +0.2 42,409 - -0.3 42,409 - -0.2 42,640 + GFF40 0.17 +0.0 42,641 - GFF40 0.17 +0.5 42,715 + GFF40 0.23 +0.4 42,715 + GFF40 0.23 +0.4 42,715 + GFF40 0.23 -0.8 42,715 + GFF40 0.23 +0.4 42,715 + GFF40 0.23 +0.4 42,715 + GFF40 0.23 -2.5 42,716 - GFF40 0.23 -0.2 42,716 - GFF40 0.23 -0.4 42,716 - GFF40 0.23 -0.3 42,716 - GFF40 0.23 +0.6 42,716 - GFF40 0.23 +0.3 42,716 - GFF40 0.23 -0.2 42,716 - GFF40 0.23 -0.2 42,716 - GFF40 0.23 +0.5 42,721 + GFF40 0.24 +0.9 42,751 - GFF40 0.27 +0.6 42,784 + GFF40 0.29 -0.2 42,784 + GFF40 0.29 -0.4 42,970 + GFF40 0.46 -1.9 43,039 + GFF40 0.52 -1.4 43,039 + GFF40 0.52 +0.0 43,039 + GFF40 0.52 -0.6 43,039 + GFF40 0.52 +0.5 43,039 + GFF40 0.52 -1.4 43,039 + GFF40 0.52 -0.4 43,039 + GFF40 0.52 -1.8 43,039 + GFF40 0.52 -0.4 43,040 - GFF40 0.52 -0.4 43,040 - GFF40 0.52 -0.5 43,165 + GFF40 0.63 -1.3 43,166 - GFF40 0.63 +0.7 43,343 - GFF40 0.78 -1.6 43,343 - GFF40 0.78 -0.3
Or see this region's nucleotide sequence