Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3573 and GFF3574 are separated by 96 nucleotides GFF3574 and GFF3575 overlap by 4 nucleotides GFF3575 and GFF3576 overlap by 4 nucleotides GFF3576 and GFF3577 overlap by 4 nucleotides
GFF3573 - Efflux ABC transporter, ATP-binding protein, at 85,350 to 86,294
GFF3573
GFF3574 - hypothetical protein, at 86,391 to 87,233
GFF3574
GFF3575 - no description, at 87,230 to 87,916
GFF3575
GFF3576 - no description, at 87,913 to 88,554
GFF3576
GFF3577 - Transcriptional regulator, LysR family, at 88,551 to 89,441
GFF3577
Position (kb) 87
88 Strain fitness (log2 ratio) -2
-1
0
1 at 86.234 kb on - strand at 86.670 kb on + strand, within GFF3574 at 86.670 kb on + strand, within GFF3574 at 86.670 kb on + strand, within GFF3574 at 86.677 kb on - strand, within GFF3574 at 86.826 kb on + strand, within GFF3574 at 86.826 kb on + strand, within GFF3574 at 86.827 kb on - strand, within GFF3574 at 86.827 kb on - strand, within GFF3574 at 86.827 kb on - strand, within GFF3574 at 86.827 kb on - strand, within GFF3574 at 86.827 kb on - strand, within GFF3574 at 86.847 kb on + strand, within GFF3574 at 86.848 kb on - strand, within GFF3574 at 86.848 kb on - strand, within GFF3574 at 86.848 kb on - strand, within GFF3574 at 86.848 kb on - strand, within GFF3574 at 86.928 kb on + strand, within GFF3574 at 86.928 kb on + strand, within GFF3574 at 86.929 kb on - strand, within GFF3574 at 87.422 kb on + strand, within GFF3575 at 87.422 kb on + strand, within GFF3575 at 87.722 kb on + strand, within GFF3575 at 87.722 kb on + strand, within GFF3575 at 87.722 kb on + strand, within GFF3575 at 87.723 kb on - strand, within GFF3575 at 87.723 kb on - strand, within GFF3575 at 87.723 kb on - strand, within GFF3575 at 88.074 kb on + strand, within GFF3576 at 88.074 kb on + strand, within GFF3576 at 88.075 kb on - strand, within GFF3576 at 88.240 kb on + strand, within GFF3576 at 88.241 kb on - strand, within GFF3576 at 88.633 kb on + strand at 88.633 kb on + strand at 88.633 kb on + strand at 88.634 kb on - strand at 88.720 kb on + strand, within GFF3577 at 88.720 kb on + strand, within GFF3577 at 88.721 kb on - strand, within GFF3577 at 88.721 kb on - strand, within GFF3577
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 86,234 - +0.5 86,670 + GFF3574 0.33 -0.4 86,670 + GFF3574 0.33 -0.2 86,670 + GFF3574 0.33 -0.9 86,677 - GFF3574 0.34 +0.6 86,826 + GFF3574 0.52 +1.1 86,826 + GFF3574 0.52 +0.1 86,827 - GFF3574 0.52 -0.3 86,827 - GFF3574 0.52 +0.7 86,827 - GFF3574 0.52 -0.8 86,827 - GFF3574 0.52 -0.6 86,827 - GFF3574 0.52 +0.6 86,847 + GFF3574 0.54 -1.2 86,848 - GFF3574 0.54 -1.1 86,848 - GFF3574 0.54 -0.5 86,848 - GFF3574 0.54 +1.6 86,848 - GFF3574 0.54 -1.4 86,928 + GFF3574 0.64 -0.5 86,928 + GFF3574 0.64 +0.1 86,929 - GFF3574 0.64 -1.1 87,422 + GFF3575 0.28 +0.3 87,422 + GFF3575 0.28 -1.3 87,722 + GFF3575 0.72 -1.3 87,722 + GFF3575 0.72 -0.5 87,722 + GFF3575 0.72 -1.2 87,723 - GFF3575 0.72 -0.2 87,723 - GFF3575 0.72 -1.9 87,723 - GFF3575 0.72 -0.5 88,074 + GFF3576 0.25 -0.3 88,074 + GFF3576 0.25 -0.3 88,075 - GFF3576 0.25 +0.1 88,240 + GFF3576 0.51 +0.1 88,241 - GFF3576 0.51 -0.8 88,633 + +0.3 88,633 + +1.1 88,633 + -1.1 88,634 - +0.6 88,720 + GFF3577 0.19 +0.6 88,720 + GFF3577 0.19 +0.1 88,721 - GFF3577 0.19 +0.0 88,721 - GFF3577 0.19 -1.2
Or see this region's nucleotide sequence