Strain Fitness in Variovorax sp. SCN45 around GFF3547

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

Per-strain Table

Position	Strand	Gene	LocusTag	Fraction	M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
50,615	-		GFF3545	0.66	-1.8
50,728	+		GFF3545	0.71	-1.5
50,728	+		GFF3545	0.71	-1.1
50,728	+		GFF3545	0.71	-1.3
50,728	+		GFF3545	0.71	-0.2
50,728	+		GFF3545	0.71	-1.8
50,728	+		GFF3545	0.71	-0.1
50,729	-		GFF3545	0.71	-0.3
50,729	-		GFF3545	0.71	-0.2
50,729	-		GFF3545	0.71	-0.5
50,729	-		GFF3545	0.71	-0.8
50,729	-		GFF3545	0.71	-0.1
50,729	-		GFF3545	0.71	-0.4
50,761	+		GFF3545	0.72	-0.0
50,761	+		GFF3545	0.72	+0.2
50,761	+		GFF3545	0.72	+0.5
50,761	+		GFF3545	0.72	+0.1
50,761	+		GFF3545	0.72	-0.5
50,761	+		GFF3545	0.72	-0.5
50,761	+		GFF3545	0.72	+0.8
50,761	+		GFF3545	0.72	+0.5
50,761	+		GFF3545	0.72	-0.8
50,761	+		GFF3545	0.72	+0.4
50,761	+		GFF3545	0.72	+0.7
50,761	+		GFF3545	0.72	+1.5
50,762	-		GFF3545	0.72	+0.3
50,762	-		GFF3545	0.72	+0.1
50,762	-		GFF3545	0.72	+0.8
50,762	-		GFF3545	0.72	-0.5
50,762	-		GFF3545	0.72	-0.6
50,762	-		GFF3545	0.72	-0.5
50,762	-		GFF3545	0.72	+0.1
50,762	-		GFF3545	0.72	-0.2
50,762	-		GFF3545	0.72	-0.6
50,762	-		GFF3545	0.72	+0.6
50,762	-		GFF3545	0.72	-0.5
50,857	+		GFF3545	0.76	-0.4
50,857	+		GFF3545	0.76	-0.9
50,857	+		GFF3545	0.76	-0.9
50,857	+		GFF3545	0.76	-1.1
50,857	+		GFF3545	0.76	+0.4
50,858	-		GFF3545	0.76	-0.2
50,858	-		GFF3545	0.76	-0.9
50,941	+		GFF3545	0.80	-0.3
50,942	-		GFF3545	0.80	+1.5
50,942	-		GFF3545	0.80	+1.5
50,942	-		GFF3545	0.80	-0.7
50,942	-		GFF3545	0.80	-0.3
50,942	-		GFF3545	0.80	-0.6
50,948	-		GFF3545	0.80	-0.1
51,136	+		GFF3545	0.88	-0.3
51,136	+		GFF3545	0.88	-0.3
51,136	+		GFF3545	0.88	-1.1
51,464	+				-0.4
51,464	+				-0.5
51,464	+				-0.9
51,465	-				-1.8
51,465	-				+0.7
51,465	-				-0.9
51,482	+				-1.4
51,482	+				-0.9
51,482	+				-1.9
51,482	+				+1.1
51,482	+				-0.4
51,482	+				+0.4
51,482	+				+0.7
51,482	+				-0.6
51,482	+				-0.1
51,483	-				-0.9
51,483	-				-0.3
51,483	-				+1.5
51,483	-				-0.1
51,483	-				-0.5
51,483	-				-0.5
51,483	-				-0.5
51,483	-				-0.8
51,568	+		GFF3547	0.18	+0.3
51,568	+		GFF3547	0.18	-0.5
51,568	+		GFF3547	0.18	-1.9
51,568	+		GFF3547	0.18	-0.8
51,568	+		GFF3547	0.18	-0.8
51,569	-		GFF3547	0.19	-0.9
51,673	+				-0.3
51,674	-				+2.1
51,703	+				-1.0
51,704	-				-3.6
51,704	-				-0.8
51,726	-				-1.5
51,765	-				+1.7
51,776	+				+2.4
51,860	+				-0.0
51,860	+				-0.1
51,860	+				-0.5
51,860	+				-0.4
51,861	-				+1.9
51,861	-				-0.8
51,861	-				+1.1
51,861	-				-0.7
51,861	-				+0.1
51,861	-				+0.3
51,861	-				-0.3
51,998	+		GFF3546	0.15	-0.0
52,073	+		GFF3546	0.19	-0.2
52,073	+		GFF3546	0.19	+0.5
52,073	+		GFF3546	0.19	+0.1
52,073	+		GFF3546	0.19	-0.4
52,073	+		GFF3546	0.19	-1.8
52,073	+		GFF3546	0.19	-0.8
52,074	-		GFF3546	0.19	-0.0
52,074	-		GFF3546	0.19	-0.9
52,074	-		GFF3546	0.19	-0.4
52,074	-		GFF3546	0.19	-0.3
52,074	-		GFF3546	0.19	-1.0
52,074	-		GFF3546	0.19	-0.4
52,250	+		GFF3546	0.28	-0.6
52,250	+		GFF3546	0.28	+0.9
52,250	+		GFF3546	0.28	-0.6
52,250	+		GFF3546	0.28	+1.1
52,250	+		GFF3546	0.28	+0.4
52,251	-		GFF3546	0.28	-0.0
52,251	-		GFF3546	0.28	-0.7
52,251	-		GFF3546	0.28	+1.1
52,289	+		GFF3546	0.30	+1.6
52,289	+		GFF3546	0.30	+0.1
52,289	+		GFF3546	0.30	-2.0
52,289	+		GFF3546	0.30	-0.6
52,290	-		GFF3546	0.30	+1.2
52,290	-		GFF3546	0.30	+1.0
52,290	-		GFF3546	0.30	-0.0
52,290	-		GFF3546	0.30	+0.5
52,290	-		GFF3546	0.30	+0.1
52,290	-		GFF3546	0.30	+0.2
52,451	+		GFF3546	0.38	+0.5
52,451	+		GFF3546	0.38	-1.1
52,451	+		GFF3546	0.38	+0.3
52,451	+		GFF3546	0.38	-0.6
52,452	-		GFF3546	0.38	+0.9
52,452	-		GFF3546	0.38	+1.1
52,463	+		GFF3546	0.38	+0.2
52,463	+		GFF3546	0.38	-0.1
52,463	+		GFF3546	0.38	-2.2
52,463	+		GFF3546	0.38	-0.9
52,464	-		GFF3546	0.38	-0.6
52,464	-		GFF3546	0.38	-1.4
52,464	-		GFF3546	0.38	-0.1
52,464	-		GFF3546	0.38	+0.5
52,464	-		GFF3546	0.38	+0.1
52,464	-		GFF3546	0.38	-0.5
52,464	-		GFF3546	0.38	-2.1
52,464	-		GFF3546	0.38	+1.0
52,464	-		GFF3546	0.38	-0.0
52,464	-		GFF3546	0.38	-0.3
52,484	+		GFF3546	0.39	-0.9
52,484	+		GFF3546	0.39	-1.1
52,484	+		GFF3546	0.39	-3.5
52,484	+		GFF3546	0.39	+0.1
52,485	-		GFF3546	0.39	-1.9
52,485	-		GFF3546	0.39	+2.1
52,485	-		GFF3546	0.39	+0.4
52,485	-		GFF3546	0.39	+0.3
52,535	+		GFF3546	0.42	-0.1
52,535	+		GFF3546	0.42	+0.5
52,535	+		GFF3546	0.42	-1.7
52,536	-		GFF3546	0.42	-1.6
52,536	-		GFF3546	0.42	-0.3
52,536	-		GFF3546	0.42	-0.4
52,536	-		GFF3546	0.42	-1.0
52,536	-		GFF3546	0.42	+0.4
52,550	+		GFF3546	0.42	-1.3
52,550	+		GFF3546	0.42	-0.5
52,550	+		GFF3546	0.42	-0.3
52,550	+		GFF3546	0.42	-1.0
52,550	+		GFF3546	0.42	+1.0
52,550	+		GFF3546	0.42	-0.5
52,550	+		GFF3546	0.42	-0.8
52,550	+		GFF3546	0.42	+0.2
52,550	+		GFF3546	0.42	-0.3
52,550	+		GFF3546	0.42	-0.6
52,550	+		GFF3546	0.42	-0.0
52,550	+		GFF3546	0.42	-1.9
52,550	+		GFF3546	0.42	+0.0
52,550	+		GFF3546	0.42	-1.3
52,550	+		GFF3546	0.42	-3.2
52,550	+		GFF3546	0.42	+0.7
52,550	+		GFF3546	0.42	-0.6
52,550	+		GFF3546	0.42	-0.2
52,550	+		GFF3546	0.42	+0.3
52,550	+		GFF3546	0.42	-0.3
52,550	+		GFF3546	0.42	+1.1
52,551	-		GFF3546	0.42	-1.8
52,551	-		GFF3546	0.42	-0.9
52,551	-		GFF3546	0.42	-0.4
52,551	-		GFF3546	0.42	-0.8
52,551	-		GFF3546	0.42	-0.8
52,551	-		GFF3546	0.42	+0.1
52,551	-		GFF3546	0.42	+0.2
52,551	-		GFF3546	0.42	-0.2
52,551	-		GFF3546	0.42	-0.3
52,551	-		GFF3546	0.42	-0.1
52,551	-		GFF3546	0.42	-2.9
52,551	-		GFF3546	0.42	-1.1
52,551	-		GFF3546	0.42	-0.8
52,551	-		GFF3546	0.42	-0.6
52,613	+		GFF3546	0.46	+1.1
52,613	+		GFF3546	0.46	-0.1
52,613	+		GFF3546	0.46	-1.1
52,613	+		GFF3546	0.46	+0.8
52,613	+		GFF3546	0.46	+0.1
52,614	-		GFF3546	0.46	+0.5
52,614	-		GFF3546	0.46	-1.1
52,614	-		GFF3546	0.46	-1.9
52,614	-		GFF3546	0.46	-0.0
52,614	-		GFF3546	0.46	+0.2
52,664	+		GFF3546	0.48	+0.1
52,664	+		GFF3546	0.48	+1.8
52,664	+		GFF3546	0.48	-0.5
52,664	+		GFF3546	0.48	-0.3
52,664	+		GFF3546	0.48	-0.5
52,664	+		GFF3546	0.48	-0.3
52,665	-		GFF3546	0.48	-0.5
52,665	-		GFF3546	0.48	-0.5