Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3495 and GFF3496 overlap by 4 nucleotides GFF3496 and GFF3497 are separated by 117 nucleotides GFF3497 and GFF3498 overlap by 4 nucleotides
GFF3495 - ABC transporter, ATP-binding protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 222 to 1,055
GFF3495
GFF3496 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 1,052 to 2,974
GFF3496
GFF3497 - Transcriptional regulator, Crp/Fnr family, at 3,092 to 3,871
GFF3497
GFF3498 - hypothetical protein, at 3,868 to 4,335
GFF3498
Position (kb)
1
2
3 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 0.241 kb on + strand at 0.242 kb on - strand at 0.308 kb on - strand, within GFF3495 at 0.830 kb on - strand, within GFF3495 at 0.830 kb on - strand, within GFF3495 at 1.179 kb on + strand at 1.204 kb on - strand at 1.405 kb on - strand, within GFF3496 at 1.460 kb on + strand, within GFF3496 at 1.551 kb on + strand, within GFF3496 at 1.551 kb on + strand, within GFF3496 at 1.551 kb on + strand, within GFF3496 at 1.551 kb on + strand, within GFF3496 at 1.552 kb on - strand, within GFF3496 at 1.552 kb on - strand, within GFF3496 at 1.552 kb on - strand, within GFF3496 at 1.662 kb on + strand, within GFF3496 at 1.707 kb on + strand, within GFF3496 at 1.707 kb on + strand, within GFF3496 at 1.708 kb on - strand, within GFF3496 at 1.708 kb on - strand, within GFF3496 at 1.708 kb on - strand, within GFF3496 at 1.836 kb on + strand, within GFF3496 at 1.837 kb on - strand, within GFF3496 at 1.837 kb on - strand, within GFF3496 at 1.861 kb on - strand, within GFF3496 at 1.861 kb on - strand, within GFF3496 at 1.893 kb on + strand, within GFF3496 at 1.935 kb on + strand, within GFF3496 at 1.936 kb on - strand, within GFF3496 at 1.984 kb on - strand, within GFF3496 at 2.007 kb on + strand, within GFF3496 at 2.091 kb on + strand, within GFF3496 at 2.091 kb on + strand, within GFF3496 at 2.178 kb on + strand, within GFF3496 at 2.178 kb on + strand, within GFF3496 at 2.179 kb on - strand, within GFF3496 at 2.179 kb on - strand, within GFF3496 at 2.181 kb on + strand, within GFF3496 at 2.310 kb on + strand, within GFF3496 at 2.311 kb on - strand, within GFF3496 at 2.311 kb on - strand, within GFF3496 at 2.430 kb on + strand, within GFF3496 at 2.431 kb on - strand, within GFF3496 at 2.481 kb on + strand, within GFF3496 at 2.481 kb on + strand, within GFF3496 at 2.481 kb on + strand, within GFF3496 at 2.481 kb on + strand, within GFF3496 at 2.481 kb on + strand, within GFF3496 at 2.482 kb on - strand, within GFF3496 at 2.482 kb on - strand, within GFF3496 at 2.482 kb on - strand, within GFF3496 at 2.485 kb on - strand, within GFF3496 at 2.535 kb on + strand, within GFF3496 at 2.535 kb on + strand, within GFF3496 at 2.536 kb on - strand, within GFF3496 at 2.536 kb on - strand, within GFF3496 at 2.560 kb on - strand, within GFF3496 at 2.560 kb on - strand, within GFF3496 at 2.586 kb on + strand, within GFF3496 at 2.586 kb on + strand, within GFF3496 at 2.587 kb on - strand, within GFF3496 at 2.736 kb on + strand, within GFF3496 at 2.736 kb on + strand, within GFF3496 at 2.787 kb on + strand at 2.787 kb on + strand at 2.787 kb on + strand at 2.787 kb on + strand at 2.788 kb on - strand at 2.788 kb on - strand at 2.788 kb on - strand at 2.788 kb on - strand at 2.788 kb on - strand at 2.788 kb on - strand at 2.788 kb on - strand at 3.021 kb on + strand at 3.021 kb on + strand at 3.021 kb on + strand at 3.021 kb on + strand at 3.021 kb on + strand at 3.022 kb on - strand at 3.022 kb on - strand at 3.022 kb on - strand at 3.072 kb on + strand at 3.073 kb on + strand at 3.073 kb on + strand at 3.073 kb on + strand at 3.073 kb on + strand at 3.073 kb on + strand at 3.073 kb on + strand at 3.074 kb on - strand at 3.074 kb on - strand at 3.074 kb on - strand at 3.074 kb on - strand at 3.296 kb on + strand, within GFF3497 at 3.296 kb on + strand, within GFF3497 at 3.296 kb on + strand, within GFF3497 at 3.296 kb on + strand, within GFF3497 at 3.297 kb on - strand, within GFF3497 at 3.297 kb on - strand, within GFF3497 at 3.297 kb on - strand, within GFF3497 at 3.297 kb on - strand, within GFF3497 at 3.297 kb on - strand, within GFF3497 at 3.297 kb on - strand, within GFF3497 at 3.365 kb on + strand, within GFF3497 at 3.365 kb on + strand, within GFF3497 at 3.365 kb on + strand, within GFF3497 at 3.365 kb on + strand, within GFF3497 at 3.366 kb on - strand, within GFF3497 at 3.366 kb on - strand, within GFF3497 at 3.366 kb on - strand, within GFF3497 at 3.431 kb on + strand, within GFF3497 at 3.432 kb on - strand, within GFF3497 at 3.432 kb on - strand, within GFF3497 at 3.647 kb on + strand, within GFF3497 at 3.647 kb on + strand, within GFF3497 at 3.647 kb on + strand, within GFF3497 at 3.695 kb on + strand, within GFF3497 at 3.695 kb on + strand, within GFF3497 at 3.696 kb on - strand, within GFF3497 at 3.696 kb on - strand, within GFF3497 at 3.773 kb on + strand, within GFF3497 at 3.773 kb on + strand, within GFF3497 at 3.774 kb on - strand, within GFF3497 at 3.833 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 241 + +0.7 242 - -0.1 308 - GFF3495 0.10 -0.3 830 - GFF3495 0.73 -0.3 830 - GFF3495 0.73 +0.2 1,179 + -0.5 1,204 - +0.6 1,405 - GFF3496 0.18 +0.1 1,460 + GFF3496 0.21 +0.6 1,551 + GFF3496 0.26 +0.3 1,551 + GFF3496 0.26 +0.5 1,551 + GFF3496 0.26 +0.5 1,551 + GFF3496 0.26 -0.2 1,552 - GFF3496 0.26 +0.5 1,552 - GFF3496 0.26 +1.5 1,552 - GFF3496 0.26 +0.4 1,662 + GFF3496 0.32 -0.3 1,707 + GFF3496 0.34 -0.0 1,707 + GFF3496 0.34 -0.3 1,708 - GFF3496 0.34 -1.3 1,708 - GFF3496 0.34 +0.9 1,708 - GFF3496 0.34 -1.1 1,836 + GFF3496 0.41 +1.5 1,837 - GFF3496 0.41 +3.4 1,837 - GFF3496 0.41 +0.2 1,861 - GFF3496 0.42 +0.5 1,861 - GFF3496 0.42 -0.2 1,893 + GFF3496 0.44 +0.5 1,935 + GFF3496 0.46 -1.2 1,936 - GFF3496 0.46 +0.7 1,984 - GFF3496 0.48 -0.9 2,007 + GFF3496 0.50 -0.7 2,091 + GFF3496 0.54 +0.6 2,091 + GFF3496 0.54 -0.2 2,178 + GFF3496 0.59 -0.2 2,178 + GFF3496 0.59 -0.9 2,179 - GFF3496 0.59 -0.6 2,179 - GFF3496 0.59 +0.9 2,181 + GFF3496 0.59 -0.2 2,310 + GFF3496 0.65 -0.3 2,311 - GFF3496 0.65 +0.0 2,311 - GFF3496 0.65 -1.1 2,430 + GFF3496 0.72 -1.0 2,431 - GFF3496 0.72 -2.0 2,481 + GFF3496 0.74 -0.2 2,481 + GFF3496 0.74 +1.1 2,481 + GFF3496 0.74 -0.8 2,481 + GFF3496 0.74 +0.3 2,481 + GFF3496 0.74 -0.0 2,482 - GFF3496 0.74 +0.7 2,482 - GFF3496 0.74 +0.0 2,482 - GFF3496 0.74 -1.3 2,485 - GFF3496 0.75 +0.0 2,535 + GFF3496 0.77 +0.8 2,535 + GFF3496 0.77 +0.9 2,536 - GFF3496 0.77 +0.5 2,536 - GFF3496 0.77 -0.4 2,560 - GFF3496 0.78 -2.2 2,560 - GFF3496 0.78 -0.6 2,586 + GFF3496 0.80 +1.8 2,586 + GFF3496 0.80 +1.1 2,587 - GFF3496 0.80 +0.7 2,736 + GFF3496 0.88 +2.8 2,736 + GFF3496 0.88 -0.5 2,787 + -0.2 2,787 + +1.5 2,787 + -0.1 2,787 + +0.5 2,788 - -0.3 2,788 - +0.7 2,788 - +0.6 2,788 - -0.1 2,788 - +0.6 2,788 - +0.1 2,788 - -0.2 3,021 + -0.5 3,021 + +0.0 3,021 + +0.5 3,021 + -1.1 3,021 + -0.1 3,022 - +0.1 3,022 - +0.9 3,022 - -0.1 3,072 + -0.2 3,073 + -0.4 3,073 + -0.5 3,073 + +0.5 3,073 + -0.9 3,073 + +1.1 3,073 + +1.8 3,074 - +1.1 3,074 - -0.5 3,074 - -0.1 3,074 - +0.5 3,296 + GFF3497 0.26 -0.5 3,296 + GFF3497 0.26 -0.9 3,296 + GFF3497 0.26 +0.1 3,296 + GFF3497 0.26 +0.1 3,297 - GFF3497 0.26 -1.0 3,297 - GFF3497 0.26 +0.1 3,297 - GFF3497 0.26 -0.4 3,297 - GFF3497 0.26 -1.5 3,297 - GFF3497 0.26 -0.5 3,297 - GFF3497 0.26 +0.3 3,365 + GFF3497 0.35 +1.5 3,365 + GFF3497 0.35 -1.8 3,365 + GFF3497 0.35 +0.1 3,365 + GFF3497 0.35 -2.1 3,366 - GFF3497 0.35 +0.4 3,366 - GFF3497 0.35 -0.5 3,366 - GFF3497 0.35 +0.0 3,431 + GFF3497 0.43 +0.1 3,432 - GFF3497 0.44 -0.9 3,432 - GFF3497 0.44 -0.4 3,647 + GFF3497 0.71 -0.4 3,647 + GFF3497 0.71 -0.8 3,647 + GFF3497 0.71 -0.1 3,695 + GFF3497 0.77 -0.9 3,695 + GFF3497 0.77 -0.1 3,696 - GFF3497 0.77 -0.3 3,696 - GFF3497 0.77 -0.1 3,773 + GFF3497 0.87 -0.6 3,773 + GFF3497 0.87 -1.2 3,774 - GFF3497 0.87 -0.0 3,833 + +0.0
Or see this region's nucleotide sequence