Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3430 and GFF3431 are separated by 186 nucleotides GFF3431 and GFF3432 are separated by 121 nucleotides
GFF3430 - Transcriptional regulator, LysR family, at 253,241 to 254,143
GFF3430
GFF3431 - Glutaryl-CoA dehydrogenase (EC 1.3.8.6), at 254,330 to 255,529
GFF3431
GFF3432 - L-carnitine dehydratase/bile acid-inducible protein F, at 255,651 to 256,889
GFF3432
Position (kb) 254
255
256 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 253.330 kb on - strand at 253.330 kb on - strand at 253.330 kb on - strand at 253.330 kb on - strand at 253.330 kb on - strand at 253.470 kb on + strand, within GFF3430 at 253.470 kb on + strand, within GFF3430 at 253.471 kb on - strand, within GFF3430 at 253.704 kb on + strand, within GFF3430 at 253.704 kb on + strand, within GFF3430 at 253.705 kb on - strand, within GFF3430 at 253.705 kb on - strand, within GFF3430 at 253.938 kb on + strand, within GFF3430 at 253.938 kb on + strand, within GFF3430 at 253.938 kb on + strand, within GFF3430 at 253.938 kb on + strand, within GFF3430 at 253.938 kb on + strand, within GFF3430 at 254.076 kb on + strand at 254.076 kb on + strand at 254.076 kb on + strand at 254.076 kb on + strand at 254.076 kb on + strand at 254.077 kb on - strand at 254.077 kb on - strand at 254.077 kb on - strand at 254.077 kb on - strand at 254.077 kb on - strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.145 kb on + strand at 254.146 kb on - strand at 254.146 kb on - strand at 254.146 kb on - strand at 254.146 kb on - strand at 254.146 kb on - strand at 254.158 kb on + strand at 254.218 kb on + strand at 254.219 kb on - strand at 254.219 kb on - strand at 254.229 kb on - strand at 254.229 kb on - strand at 254.273 kb on + strand at 254.274 kb on - strand at 254.426 kb on + strand at 254.426 kb on + strand at 254.426 kb on + strand at 254.427 kb on - strand at 254.546 kb on + strand, within GFF3431 at 254.546 kb on + strand, within GFF3431 at 254.567 kb on + strand, within GFF3431 at 254.567 kb on + strand, within GFF3431 at 254.567 kb on + strand, within GFF3431 at 254.568 kb on - strand, within GFF3431 at 254.568 kb on - strand, within GFF3431 at 254.568 kb on - strand, within GFF3431 at 254.568 kb on - strand, within GFF3431 at 254.576 kb on + strand, within GFF3431 at 254.577 kb on - strand, within GFF3431 at 255.161 kb on + strand, within GFF3431 at 255.162 kb on - strand, within GFF3431 at 255.162 kb on - strand, within GFF3431 at 255.455 kb on + strand at 255.455 kb on + strand at 255.455 kb on + strand at 255.455 kb on + strand at 255.456 kb on - strand at 255.456 kb on - strand at 255.456 kb on - strand at 255.456 kb on - strand at 255.527 kb on + strand at 255.528 kb on - strand at 255.528 kb on - strand at 255.540 kb on + strand at 255.540 kb on + strand at 255.540 kb on + strand at 255.540 kb on + strand at 255.540 kb on + strand at 255.540 kb on + strand at 255.541 kb on - strand at 255.541 kb on - strand at 255.541 kb on - strand at 255.541 kb on - strand at 255.541 kb on - strand at 255.553 kb on + strand at 255.553 kb on + strand at 255.554 kb on - strand at 255.855 kb on + strand, within GFF3432 at 255.855 kb on + strand, within GFF3432 at 255.855 kb on + strand, within GFF3432 at 255.855 kb on + strand, within GFF3432 at 255.855 kb on + strand, within GFF3432 at 255.856 kb on - strand, within GFF3432 at 255.856 kb on - strand, within GFF3432 at 255.856 kb on - strand, within GFF3432 at 255.856 kb on - strand, within GFF3432 at 255.856 kb on - strand, within GFF3432 at 255.993 kb on + strand, within GFF3432 at 255.993 kb on + strand, within GFF3432 at 255.994 kb on - strand, within GFF3432 at 255.994 kb on - strand, within GFF3432 at 256.041 kb on + strand, within GFF3432 at 256.041 kb on + strand, within GFF3432 at 256.041 kb on + strand, within GFF3432 at 256.041 kb on + strand, within GFF3432 at 256.041 kb on + strand, within GFF3432 at 256.042 kb on - strand, within GFF3432 at 256.042 kb on - strand, within GFF3432 at 256.215 kb on + strand, within GFF3432 at 256.215 kb on + strand, within GFF3432 at 256.216 kb on - strand, within GFF3432 at 256.395 kb on + strand, within GFF3432
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 253,330 - -1.1 253,330 - -0.7 253,330 - -0.3 253,330 - -0.8 253,330 - +0.7 253,470 + GFF3430 0.25 -0.6 253,470 + GFF3430 0.25 -1.2 253,471 - GFF3430 0.25 -0.5 253,704 + GFF3430 0.51 +2.1 253,704 + GFF3430 0.51 -0.4 253,705 - GFF3430 0.51 -1.0 253,705 - GFF3430 0.51 -0.1 253,938 + GFF3430 0.77 -0.8 253,938 + GFF3430 0.77 -0.1 253,938 + GFF3430 0.77 -0.8 253,938 + GFF3430 0.77 +0.5 253,938 + GFF3430 0.77 -0.9 254,076 + -0.3 254,076 + +0.1 254,076 + -0.4 254,076 + +0.4 254,076 + -1.4 254,077 - -0.1 254,077 - -1.3 254,077 - -0.6 254,077 - -2.0 254,077 - -0.1 254,145 + -1.0 254,145 + +0.7 254,145 + +0.1 254,145 + +0.3 254,145 + -1.3 254,145 + +0.7 254,145 + -0.2 254,145 + +0.5 254,145 + +1.1 254,145 + +0.6 254,145 + -2.5 254,146 - -0.7 254,146 - -0.1 254,146 - +0.1 254,146 - -1.4 254,146 - +0.4 254,158 + -0.3 254,218 + -0.2 254,219 - -0.2 254,219 - -0.7 254,229 - +2.6 254,229 - -0.1 254,273 + +0.2 254,274 - -0.3 254,426 + +0.9 254,426 + -1.2 254,426 + +0.8 254,427 - +0.4 254,546 + GFF3431 0.18 +0.9 254,546 + GFF3431 0.18 -0.3 254,567 + GFF3431 0.20 +0.3 254,567 + GFF3431 0.20 +0.9 254,567 + GFF3431 0.20 +0.3 254,568 - GFF3431 0.20 +1.2 254,568 - GFF3431 0.20 +0.0 254,568 - GFF3431 0.20 +0.3 254,568 - GFF3431 0.20 +0.1 254,576 + GFF3431 0.20 +1.3 254,577 - GFF3431 0.21 -0.3 255,161 + GFF3431 0.69 -0.1 255,162 - GFF3431 0.69 +1.1 255,162 - GFF3431 0.69 +0.5 255,455 + +0.4 255,455 + -0.1 255,455 + -0.1 255,455 + +0.2 255,456 - -0.1 255,456 - +0.1 255,456 - +0.1 255,456 - +0.5 255,527 + +0.5 255,528 - -0.5 255,528 - -0.5 255,540 + -1.2 255,540 + +1.1 255,540 + +0.3 255,540 + -1.3 255,540 + +1.1 255,540 + +0.1 255,541 - -0.6 255,541 - -0.9 255,541 - -0.6 255,541 - +0.2 255,541 - +1.7 255,553 + -1.7 255,553 + +0.2 255,554 - +0.1 255,855 + GFF3432 0.16 -1.4 255,855 + GFF3432 0.16 -0.4 255,855 + GFF3432 0.16 +0.1 255,855 + GFF3432 0.16 +0.5 255,855 + GFF3432 0.16 -0.6 255,856 - GFF3432 0.17 -2.9 255,856 - GFF3432 0.17 +0.6 255,856 - GFF3432 0.17 +0.1 255,856 - GFF3432 0.17 -0.9 255,856 - GFF3432 0.17 +0.6 255,993 + GFF3432 0.28 -0.3 255,993 + GFF3432 0.28 -0.1 255,994 - GFF3432 0.28 -1.4 255,994 - GFF3432 0.28 +0.3 256,041 + GFF3432 0.31 +0.6 256,041 + GFF3432 0.31 -0.0 256,041 + GFF3432 0.31 -1.2 256,041 + GFF3432 0.31 -1.3 256,041 + GFF3432 0.31 +0.1 256,042 - GFF3432 0.32 -0.5 256,042 - GFF3432 0.32 -1.7 256,215 + GFF3432 0.46 -0.7 256,215 + GFF3432 0.46 +0.7 256,216 - GFF3432 0.46 -0.4 256,395 + GFF3432 0.60 -0.7
Or see this region's nucleotide sequence