Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF297 and GFF298 overlap by 4 nucleotides GFF298 and GFF299 overlap by 4 nucleotides GFF299 and GFF300 overlap by 1 nucleotides GFF300 and GFF301 overlap by 4 nucleotides
GFF297 - FIG00978250: hypothetical protein, at 331,787 to 332,500
GFF297
GFF298 - 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase (EC 2.1.1.177), at 332,497 to 332,964
GFF298
GFF299 - Ribosomal silencing factor RsfA, at 332,961 to 333,647
GFF299
GFF300 - Nicotinate-nucleotide adenylyltransferase (EC 2.7.7.18), at 333,647 to 334,279
GFF300
GFF301 - Coproporphyrinogen III oxidase, aerobic (EC 1.3.3.3), at 334,276 to 335,193
GFF301
Position (kb) 332
333
334 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 332.095 kb on + strand, within GFF297 at 332.095 kb on + strand, within GFF297 at 332.095 kb on + strand, within GFF297 at 332.095 kb on + strand, within GFF297 at 332.095 kb on + strand, within GFF297 at 332.096 kb on - strand, within GFF297 at 332.096 kb on - strand, within GFF297 at 332.096 kb on - strand, within GFF297 at 332.105 kb on + strand, within GFF297 at 332.106 kb on - strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.148 kb on + strand, within GFF297 at 332.149 kb on - strand, within GFF297 at 332.149 kb on - strand, within GFF297 at 332.149 kb on - strand, within GFF297 at 332.149 kb on - strand, within GFF297 at 332.150 kb on - strand, within GFF297 at 332.150 kb on - strand, within GFF297 at 332.454 kb on + strand at 332.454 kb on + strand at 332.454 kb on + strand at 332.510 kb on + strand at 332.510 kb on + strand at 332.510 kb on + strand at 332.510 kb on + strand at 332.510 kb on + strand at 332.511 kb on - strand at 332.511 kb on - strand at 332.511 kb on - strand at 332.511 kb on - strand at 332.543 kb on + strand at 332.810 kb on + strand, within GFF298 at 332.810 kb on + strand, within GFF298 at 332.810 kb on + strand, within GFF298 at 332.810 kb on + strand, within GFF298 at 332.810 kb on + strand, within GFF298 at 332.810 kb on + strand, within GFF298 at 332.810 kb on + strand, within GFF298 at 332.811 kb on - strand, within GFF298 at 332.811 kb on - strand, within GFF298 at 332.811 kb on - strand, within GFF298 at 332.811 kb on - strand, within GFF298 at 332.897 kb on + strand, within GFF298 at 332.897 kb on + strand, within GFF298 at 332.898 kb on - strand, within GFF298 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.331 kb on + strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.332 kb on - strand, within GFF299 at 333.334 kb on + strand, within GFF299 at 333.334 kb on + strand, within GFF299 at 333.334 kb on + strand, within GFF299 at 333.334 kb on + strand, within GFF299 at 333.334 kb on + strand, within GFF299 at 333.334 kb on + strand, within GFF299 at 333.334 kb on + strand, within GFF299 at 333.334 kb on + strand, within GFF299 at 333.335 kb on - strand, within GFF299 at 333.335 kb on - strand, within GFF299 at 333.335 kb on - strand, within GFF299 at 333.335 kb on - strand, within GFF299 at 333.335 kb on - strand, within GFF299 at 333.552 kb on + strand, within GFF299 at 333.553 kb on - strand, within GFF299 at 333.553 kb on - strand, within GFF299 at 333.661 kb on - strand at 333.678 kb on + strand at 333.679 kb on + strand at 333.679 kb on + strand at 333.680 kb on - strand at 333.680 kb on - strand at 333.680 kb on - strand at 333.680 kb on - strand at 333.680 kb on - strand at 333.680 kb on - strand at 333.681 kb on + strand at 333.766 kb on + strand, within GFF300 at 333.766 kb on + strand, within GFF300 at 333.766 kb on + strand, within GFF300 at 333.767 kb on - strand, within GFF300
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 332,095 + GFF297 0.43 -0.7 332,095 + GFF297 0.43 +1.4 332,095 + GFF297 0.43 -1.5 332,095 + GFF297 0.43 -1.1 332,095 + GFF297 0.43 -1.3 332,096 - GFF297 0.43 -0.7 332,096 - GFF297 0.43 +0.4 332,096 - GFF297 0.43 -2.5 332,105 + GFF297 0.45 -0.4 332,106 - GFF297 0.45 +1.1 332,148 + GFF297 0.51 -0.4 332,148 + GFF297 0.51 -1.1 332,148 + GFF297 0.51 -0.5 332,148 + GFF297 0.51 -0.6 332,148 + GFF297 0.51 -1.4 332,148 + GFF297 0.51 +0.3 332,148 + GFF297 0.51 +1.1 332,148 + GFF297 0.51 +1.0 332,148 + GFF297 0.51 -0.1 332,148 + GFF297 0.51 -0.2 332,148 + GFF297 0.51 +0.9 332,149 - GFF297 0.51 -0.3 332,149 - GFF297 0.51 -0.6 332,149 - GFF297 0.51 -1.1 332,149 - GFF297 0.51 +0.8 332,150 - GFF297 0.51 -1.1 332,150 - GFF297 0.51 -0.7 332,454 + -0.4 332,454 + +0.2 332,454 + -1.4 332,510 + +0.4 332,510 + -0.0 332,510 + +1.0 332,510 + -1.6 332,510 + +0.3 332,511 - +0.4 332,511 - +0.2 332,511 - +0.3 332,511 - +0.3 332,543 + +0.3 332,810 + GFF298 0.67 -0.7 332,810 + GFF298 0.67 -0.6 332,810 + GFF298 0.67 +0.3 332,810 + GFF298 0.67 +0.4 332,810 + GFF298 0.67 -0.5 332,810 + GFF298 0.67 -1.0 332,810 + GFF298 0.67 -0.4 332,811 - GFF298 0.67 +0.2 332,811 - GFF298 0.67 -0.9 332,811 - GFF298 0.67 +0.8 332,811 - GFF298 0.67 +0.5 332,897 + GFF298 0.85 -0.4 332,897 + GFF298 0.85 -0.1 332,898 - GFF298 0.86 -0.1 333,331 + GFF299 0.54 +0.7 333,331 + GFF299 0.54 -1.1 333,331 + GFF299 0.54 -0.5 333,331 + GFF299 0.54 -1.0 333,331 + GFF299 0.54 -1.9 333,331 + GFF299 0.54 -1.9 333,331 + GFF299 0.54 -0.4 333,331 + GFF299 0.54 -0.3 333,331 + GFF299 0.54 +0.3 333,331 + GFF299 0.54 -0.9 333,331 + GFF299 0.54 -0.6 333,331 + GFF299 0.54 -1.5 333,331 + GFF299 0.54 -0.9 333,331 + GFF299 0.54 -0.6 333,332 - GFF299 0.54 -1.4 333,332 - GFF299 0.54 +0.4 333,332 - GFF299 0.54 +1.7 333,332 - GFF299 0.54 +0.1 333,332 - GFF299 0.54 -0.1 333,332 - GFF299 0.54 -0.6 333,332 - GFF299 0.54 -1.8 333,332 - GFF299 0.54 -0.3 333,332 - GFF299 0.54 -0.7 333,332 - GFF299 0.54 -0.4 333,332 - GFF299 0.54 -0.6 333,332 - GFF299 0.54 -0.9 333,334 + GFF299 0.54 -0.6 333,334 + GFF299 0.54 -2.8 333,334 + GFF299 0.54 -0.6 333,334 + GFF299 0.54 -1.7 333,334 + GFF299 0.54 -0.4 333,334 + GFF299 0.54 -0.6 333,334 + GFF299 0.54 -1.6 333,334 + GFF299 0.54 +0.3 333,335 - GFF299 0.54 -0.6 333,335 - GFF299 0.54 +2.4 333,335 - GFF299 0.54 -0.2 333,335 - GFF299 0.54 -0.1 333,335 - GFF299 0.54 +0.6 333,552 + GFF299 0.86 -0.3 333,553 - GFF299 0.86 -2.5 333,553 - GFF299 0.86 -0.6 333,661 - -1.5 333,678 + -1.5 333,679 + -1.9 333,679 + -0.9 333,680 - -0.9 333,680 - +1.4 333,680 - -0.1 333,680 - -1.3 333,680 - -1.1 333,680 - -2.6 333,681 + -0.6 333,766 + GFF300 0.19 -2.3 333,766 + GFF300 0.19 -1.1 333,766 + GFF300 0.19 -1.4 333,767 - GFF300 0.19 -1.6
Or see this region's nucleotide sequence