Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2907 and GFF2908 are separated by 2 nucleotides GFF2908 and GFF2909 are separated by 38 nucleotides
GFF2907 - Glucarate dehydratase (EC 4.2.1.40), at 334,535 to 335,893
GFF2907
GFF2908 - BUG/TctC family periplasmic protein, at 335,896 to 336,897
GFF2908
GFF2909 - 5-dehydro-4-deoxyglucarate dehydratase (EC 4.2.1.41), at 336,936 to 337,847
GFF2909
Position (kb) 335
336
337 Strain fitness (log2 ratio) -1
0
1
2 at 335.100 kb on + strand, within GFF2907 at 335.101 kb on - strand, within GFF2907 at 335.382 kb on + strand, within GFF2907 at 335.382 kb on + strand, within GFF2907 at 335.415 kb on + strand, within GFF2907 at 335.415 kb on + strand, within GFF2907 at 335.416 kb on - strand, within GFF2907 at 335.438 kb on + strand, within GFF2907 at 335.887 kb on + strand at 335.887 kb on + strand at 335.888 kb on - strand at 336.122 kb on + strand, within GFF2908 at 336.122 kb on + strand, within GFF2908 at 336.123 kb on - strand, within GFF2908 at 336.123 kb on - strand, within GFF2908 at 336.123 kb on - strand, within GFF2908 at 336.317 kb on + strand, within GFF2908 at 336.318 kb on - strand, within GFF2908 at 336.318 kb on - strand, within GFF2908 at 336.318 kb on - strand, within GFF2908 at 336.518 kb on + strand, within GFF2908 at 336.518 kb on + strand, within GFF2908 at 336.518 kb on + strand, within GFF2908 at 336.518 kb on + strand, within GFF2908 at 336.519 kb on - strand, within GFF2908 at 336.519 kb on - strand, within GFF2908 at 336.543 kb on - strand, within GFF2908 at 336.608 kb on + strand, within GFF2908 at 336.609 kb on - strand, within GFF2908 at 336.609 kb on - strand, within GFF2908 at 336.874 kb on - strand at 337.103 kb on - strand, within GFF2909 at 337.168 kb on + strand, within GFF2909 at 337.168 kb on + strand, within GFF2909 at 337.168 kb on + strand, within GFF2909 at 337.169 kb on - strand, within GFF2909 at 337.169 kb on - strand, within GFF2909 at 337.217 kb on - strand, within GFF2909 at 337.241 kb on - strand, within GFF2909 at 337.241 kb on - strand, within GFF2909 at 337.252 kb on + strand, within GFF2909 at 337.252 kb on + strand, within GFF2909 at 337.252 kb on + strand, within GFF2909 at 337.253 kb on - strand, within GFF2909 at 337.253 kb on - strand, within GFF2909 at 337.283 kb on - strand, within GFF2909 at 337.309 kb on + strand, within GFF2909 at 337.426 kb on + strand, within GFF2909 at 337.427 kb on - strand, within GFF2909 at 337.507 kb on + strand, within GFF2909 at 337.507 kb on + strand, within GFF2909 at 337.508 kb on - strand, within GFF2909 at 337.508 kb on - strand, within GFF2909 at 337.508 kb on - strand, within GFF2909 at 337.750 kb on + strand, within GFF2909 at 337.750 kb on + strand, within GFF2909 at 337.751 kb on - strand, within GFF2909 at 337.751 kb on - strand, within GFF2909
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 335,100 + GFF2907 0.42 -0.4 335,101 - GFF2907 0.42 -1.4 335,382 + GFF2907 0.62 +0.6 335,382 + GFF2907 0.62 +0.9 335,415 + GFF2907 0.65 -0.3 335,415 + GFF2907 0.65 +0.9 335,416 - GFF2907 0.65 -1.6 335,438 + GFF2907 0.66 +0.5 335,887 + -0.3 335,887 + +1.6 335,888 - -0.1 336,122 + GFF2908 0.23 +1.6 336,122 + GFF2908 0.23 -0.8 336,123 - GFF2908 0.23 +0.6 336,123 - GFF2908 0.23 -0.8 336,123 - GFF2908 0.23 +0.2 336,317 + GFF2908 0.42 +0.9 336,318 - GFF2908 0.42 +0.3 336,318 - GFF2908 0.42 -0.1 336,318 - GFF2908 0.42 +0.4 336,518 + GFF2908 0.62 +1.6 336,518 + GFF2908 0.62 +0.1 336,518 + GFF2908 0.62 -0.1 336,518 + GFF2908 0.62 +2.1 336,519 - GFF2908 0.62 +0.4 336,519 - GFF2908 0.62 -0.5 336,543 - GFF2908 0.65 -0.2 336,608 + GFF2908 0.71 -0.2 336,609 - GFF2908 0.71 -0.4 336,609 - GFF2908 0.71 -0.2 336,874 - -0.5 337,103 - GFF2909 0.18 -1.6 337,168 + GFF2909 0.25 +0.3 337,168 + GFF2909 0.25 -0.4 337,168 + GFF2909 0.25 +0.1 337,169 - GFF2909 0.26 -0.4 337,169 - GFF2909 0.26 -0.3 337,217 - GFF2909 0.31 +0.7 337,241 - GFF2909 0.33 +0.2 337,241 - GFF2909 0.33 -0.8 337,252 + GFF2909 0.35 +0.6 337,252 + GFF2909 0.35 -0.2 337,252 + GFF2909 0.35 -1.6 337,253 - GFF2909 0.35 +0.0 337,253 - GFF2909 0.35 -1.0 337,283 - GFF2909 0.38 -0.5 337,309 + GFF2909 0.41 -1.5 337,426 + GFF2909 0.54 -0.4 337,427 - GFF2909 0.54 +0.2 337,507 + GFF2909 0.63 -0.5 337,507 + GFF2909 0.63 -1.2 337,508 - GFF2909 0.63 +0.5 337,508 - GFF2909 0.63 -0.9 337,508 - GFF2909 0.63 -0.4 337,750 + GFF2909 0.89 +0.4 337,750 + GFF2909 0.89 -0.2 337,751 - GFF2909 0.89 +0.4 337,751 - GFF2909 0.89 +0.2
Or see this region's nucleotide sequence