Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2714 and GFF2715 are separated by 124 nucleotides GFF2715 and GFF2716 are separated by 5 nucleotides GFF2716 and GFF2717 are separated by 19 nucleotides
GFF2714 - 2-ketogluconate utilization repressor PtxS, at 147,649 to 148,674
GFF2714
GFF2715 - 2-ketogluconate 6-phosphate reductase (EC 1.1.1.43), at 148,799 to 149,782
GFF2715
GFF2716 - 2-dehydro-3-deoxygluconokinase (EC 2.7.1.45), at 149,788 to 150,741
GFF2716
GFF2717 - Epimerase KguE, at 150,761 to 151,510
GFF2717
Position (kb)
148
149
150 Strain fitness (log2 ratio)
-2
-1
0
1 at 148.115 kb on + strand, within GFF2714 at 148.116 kb on - strand, within GFF2714 at 148.116 kb on - strand, within GFF2714 at 148.116 kb on - strand, within GFF2714 at 148.389 kb on - strand, within GFF2714 at 148.389 kb on - strand, within GFF2714 at 148.524 kb on - strand, within GFF2714 at 148.524 kb on - strand, within GFF2714 at 148.818 kb on + strand at 149.262 kb on + strand, within GFF2715 at 149.262 kb on + strand, within GFF2715 at 149.262 kb on + strand, within GFF2715 at 149.263 kb on - strand, within GFF2715 at 149.544 kb on + strand, within GFF2715 at 149.544 kb on + strand, within GFF2715 at 149.544 kb on + strand, within GFF2715 at 149.545 kb on - strand, within GFF2715 at 150.023 kb on + strand, within GFF2716 at 150.023 kb on + strand, within GFF2716 at 150.024 kb on - strand, within GFF2716 at 150.024 kb on - strand, within GFF2716 at 150.024 kb on - strand, within GFF2716 at 150.026 kb on + strand, within GFF2716 at 150.414 kb on - strand, within GFF2716 at 150.414 kb on - strand, within GFF2716 at 150.729 kb on + strand at 150.729 kb on + strand at 150.729 kb on + strand at 150.762 kb on + strand at 150.763 kb on - strand at 150.763 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 148,115 + GFF2714 0.45 -0.1 148,116 - GFF2714 0.46 +0.2 148,116 - GFF2714 0.46 -0.8 148,116 - GFF2714 0.46 -1.1 148,389 - GFF2714 0.72 -0.9 148,389 - GFF2714 0.72 -1.0 148,524 - GFF2714 0.85 -0.0 148,524 - GFF2714 0.85 -0.4 148,818 + +0.9 149,262 + GFF2715 0.47 -0.2 149,262 + GFF2715 0.47 -1.8 149,262 + GFF2715 0.47 -0.4 149,263 - GFF2715 0.47 -0.4 149,544 + GFF2715 0.76 -0.1 149,544 + GFF2715 0.76 -1.3 149,544 + GFF2715 0.76 +0.2 149,545 - GFF2715 0.76 +0.8 150,023 + GFF2716 0.25 +0.2 150,023 + GFF2716 0.25 +0.2 150,024 - GFF2716 0.25 -0.8 150,024 - GFF2716 0.25 -1.7 150,024 - GFF2716 0.25 -0.1 150,026 + GFF2716 0.25 -0.3 150,414 - GFF2716 0.66 -0.4 150,414 - GFF2716 0.66 +0.3 150,729 + -0.0 150,729 + -0.7 150,729 + +1.0 150,762 + +0.7 150,763 - +0.7 150,763 - +0.2
Or see this region's nucleotide sequence