Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2654 and GFF2655 overlap by 4 nucleotides GFF2655 and GFF2656 are separated by 155 nucleotides
GFF2654 - Efflux ABC transporter for glutathione/L-cysteine, essential for assembly of bd-type respiratory oxidases => CydC subunit, at 73,777 to 75,492
GFF2654
GFF2655 - Efflux ABC transporter for glutathione/L-cysteine, essential for assembly of bd-type respiratory oxidases => CydD subunit, at 75,489 to 77,177
GFF2655
GFF2656 - Acyltransferase 3, at 77,333 to 78,508
GFF2656
Position (kb) 75
76
77
78 Strain fitness (log2 ratio) -2
-1
0
1 at 74.583 kb on + strand, within GFF2654 at 75.054 kb on - strand, within GFF2654 at 75.054 kb on - strand, within GFF2654 at 75.054 kb on - strand, within GFF2654 at 75.094 kb on + strand, within GFF2654 at 75.094 kb on + strand, within GFF2654 at 75.221 kb on + strand, within GFF2654 at 75.378 kb on + strand at 75.378 kb on + strand at 75.378 kb on + strand at 75.379 kb on - strand at 75.379 kb on - strand at 76.281 kb on + strand, within GFF2655 at 76.402 kb on + strand, within GFF2655 at 76.402 kb on + strand, within GFF2655 at 76.402 kb on + strand, within GFF2655 at 76.402 kb on + strand, within GFF2655 at 76.402 kb on + strand, within GFF2655 at 76.402 kb on + strand, within GFF2655 at 76.402 kb on + strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.403 kb on - strand, within GFF2655 at 76.724 kb on - strand, within GFF2655 at 76.724 kb on - strand, within GFF2655 at 77.348 kb on + strand at 77.349 kb on - strand at 77.349 kb on - strand at 77.425 kb on - strand at 77.565 kb on + strand, within GFF2656 at 77.805 kb on + strand, within GFF2656 at 77.805 kb on + strand, within GFF2656 at 77.806 kb on - strand, within GFF2656 at 77.806 kb on - strand, within GFF2656 at 77.806 kb on - strand, within GFF2656 at 77.821 kb on - strand, within GFF2656 at 78.015 kb on + strand, within GFF2656 at 78.016 kb on - strand, within GFF2656 at 78.042 kb on + strand, within GFF2656 at 78.043 kb on - strand, within GFF2656 at 78.094 kb on + strand, within GFF2656 at 78.094 kb on + strand, within GFF2656 at 78.094 kb on + strand, within GFF2656 at 78.094 kb on + strand, within GFF2656 at 78.094 kb on + strand, within GFF2656 at 78.094 kb on + strand, within GFF2656 at 78.095 kb on - strand, within GFF2656 at 78.095 kb on - strand, within GFF2656 at 78.095 kb on - strand, within GFF2656 at 78.095 kb on - strand, within GFF2656 at 78.095 kb on - strand, within GFF2656
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 74,583 + GFF2654 0.47 -0.7 75,054 - GFF2654 0.74 +0.9 75,054 - GFF2654 0.74 -0.5 75,054 - GFF2654 0.74 -0.0 75,094 + GFF2654 0.77 -0.1 75,094 + GFF2654 0.77 -0.0 75,221 + GFF2654 0.84 -0.6 75,378 + -0.1 75,378 + -0.6 75,378 + -0.5 75,379 - -0.1 75,379 - -0.1 76,281 + GFF2655 0.47 +0.8 76,402 + GFF2655 0.54 +0.7 76,402 + GFF2655 0.54 -0.5 76,402 + GFF2655 0.54 -0.0 76,402 + GFF2655 0.54 -1.9 76,402 + GFF2655 0.54 -0.3 76,402 + GFF2655 0.54 -0.5 76,402 + GFF2655 0.54 +0.2 76,403 - GFF2655 0.54 +0.1 76,403 - GFF2655 0.54 +1.1 76,403 - GFF2655 0.54 -0.4 76,403 - GFF2655 0.54 +0.0 76,403 - GFF2655 0.54 +0.0 76,403 - GFF2655 0.54 -0.6 76,403 - GFF2655 0.54 -0.6 76,403 - GFF2655 0.54 +1.0 76,724 - GFF2655 0.73 +0.3 76,724 - GFF2655 0.73 -0.0 77,348 + +0.3 77,349 - -0.8 77,349 - -0.1 77,425 - -0.1 77,565 + GFF2656 0.20 +0.2 77,805 + GFF2656 0.40 -1.0 77,805 + GFF2656 0.40 +0.3 77,806 - GFF2656 0.40 -0.8 77,806 - GFF2656 0.40 -0.3 77,806 - GFF2656 0.40 -0.5 77,821 - GFF2656 0.41 -0.3 78,015 + GFF2656 0.58 +0.2 78,016 - GFF2656 0.58 -0.9 78,042 + GFF2656 0.60 -0.1 78,043 - GFF2656 0.60 +0.2 78,094 + GFF2656 0.65 -1.1 78,094 + GFF2656 0.65 +0.2 78,094 + GFF2656 0.65 -1.2 78,094 + GFF2656 0.65 -0.8 78,094 + GFF2656 0.65 -0.7 78,094 + GFF2656 0.65 -0.4 78,095 - GFF2656 0.65 +0.3 78,095 - GFF2656 0.65 +0.6 78,095 - GFF2656 0.65 -0.5 78,095 - GFF2656 0.65 +0.6 78,095 - GFF2656 0.65 +0.5
Or see this region's nucleotide sequence