Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF25 and GFF26 are separated by 134 nucleotides GFF26 and GFF27 overlap by 4 nucleotides GFF27 and GFF28 are separated by 14 nucleotides
GFF25 - Alcohol dehydrogenase (EC 1.1.1.1), at 29,212 to 30,348
GFF25
GFF26 - Esterase/lipase, at 30,483 to 31,463
GFF26
GFF27 - 4-hydroxybenzoyl-CoA thioesterase family active site, at 31,460 to 32,335
GFF27
GFF28 - hypothetical protein, at 32,350 to 33,246
GFF28
Position (kb)
30
31
32 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 30.003 kb on - strand, within GFF25 at 30.003 kb on - strand, within GFF25 at 30.003 kb on - strand, within GFF25 at 30.003 kb on - strand, within GFF25 at 30.003 kb on - strand, within GFF25 at 30.110 kb on + strand, within GFF25 at 30.111 kb on - strand, within GFF25 at 30.111 kb on - strand, within GFF25 at 30.332 kb on + strand at 30.333 kb on - strand at 30.333 kb on - strand at 30.394 kb on + strand at 30.395 kb on - strand at 30.476 kb on + strand at 30.631 kb on - strand, within GFF26 at 30.735 kb on + strand, within GFF26 at 30.735 kb on + strand, within GFF26 at 30.771 kb on + strand, within GFF26 at 31.080 kb on + strand, within GFF26 at 31.081 kb on - strand, within GFF26 at 31.081 kb on - strand, within GFF26 at 31.179 kb on + strand, within GFF26 at 31.179 kb on + strand, within GFF26 at 31.180 kb on - strand, within GFF26 at 31.180 kb on - strand, within GFF26 at 31.180 kb on - strand, within GFF26 at 31.180 kb on - strand, within GFF26 at 31.309 kb on - strand, within GFF26 at 31.356 kb on + strand, within GFF26 at 31.562 kb on + strand, within GFF27 at 31.562 kb on + strand, within GFF27 at 31.563 kb on - strand, within GFF27 at 31.563 kb on - strand, within GFF27 at 31.563 kb on - strand, within GFF27 at 31.563 kb on - strand, within GFF27 at 31.563 kb on - strand, within GFF27 at 31.563 kb on - strand, within GFF27 at 31.563 kb on - strand, within GFF27 at 31.571 kb on + strand, within GFF27 at 31.595 kb on + strand, within GFF27 at 31.619 kb on + strand, within GFF27 at 31.620 kb on - strand, within GFF27 at 31.655 kb on + strand, within GFF27 at 31.655 kb on + strand, within GFF27 at 31.656 kb on - strand, within GFF27 at 31.811 kb on + strand, within GFF27 at 31.811 kb on + strand, within GFF27 at 31.811 kb on + strand, within GFF27 at 31.811 kb on + strand, within GFF27 at 31.811 kb on + strand, within GFF27 at 31.811 kb on + strand, within GFF27 at 31.811 kb on + strand, within GFF27 at 31.812 kb on - strand, within GFF27 at 31.812 kb on - strand, within GFF27 at 31.812 kb on - strand, within GFF27 at 31.812 kb on - strand, within GFF27 at 31.812 kb on - strand, within GFF27 at 31.883 kb on + strand, within GFF27 at 31.883 kb on + strand, within GFF27 at 32.249 kb on + strand at 32.249 kb on + strand at 32.249 kb on + strand at 32.249 kb on + strand at 32.250 kb on - strand at 32.250 kb on - strand at 32.250 kb on - strand at 32.250 kb on - strand at 32.250 kb on - strand at 32.264 kb on + strand at 32.264 kb on + strand at 32.264 kb on + strand at 32.265 kb on - strand at 32.379 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 30,003 - GFF25 0.70 +0.8 30,003 - GFF25 0.70 +0.2 30,003 - GFF25 0.70 +0.6 30,003 - GFF25 0.70 +0.3 30,003 - GFF25 0.70 -0.8 30,110 + GFF25 0.79 +1.0 30,111 - GFF25 0.79 -0.1 30,111 - GFF25 0.79 +0.0 30,332 + -0.3 30,333 - +1.2 30,333 - -0.1 30,394 + +0.3 30,395 - +1.1 30,476 + +1.7 30,631 - GFF26 0.15 -1.1 30,735 + GFF26 0.26 -1.5 30,735 + GFF26 0.26 -1.3 30,771 + GFF26 0.29 +0.1 31,080 + GFF26 0.61 -0.6 31,081 - GFF26 0.61 -1.9 31,081 - GFF26 0.61 -0.0 31,179 + GFF26 0.71 -0.1 31,179 + GFF26 0.71 -1.7 31,180 - GFF26 0.71 -0.7 31,180 - GFF26 0.71 +0.1 31,180 - GFF26 0.71 +1.5 31,180 - GFF26 0.71 +0.2 31,309 - GFF26 0.84 -1.1 31,356 + GFF26 0.89 -0.7 31,562 + GFF27 0.12 +0.5 31,562 + GFF27 0.12 -0.5 31,563 - GFF27 0.12 +1.7 31,563 - GFF27 0.12 +0.3 31,563 - GFF27 0.12 +0.4 31,563 - GFF27 0.12 -0.3 31,563 - GFF27 0.12 -1.2 31,563 - GFF27 0.12 -1.1 31,563 - GFF27 0.12 -1.0 31,571 + GFF27 0.13 -0.3 31,595 + GFF27 0.15 -0.7 31,619 + GFF27 0.18 +0.3 31,620 - GFF27 0.18 +0.8 31,655 + GFF27 0.22 -0.6 31,655 + GFF27 0.22 -0.9 31,656 - GFF27 0.22 +1.5 31,811 + GFF27 0.40 +0.5 31,811 + GFF27 0.40 +0.2 31,811 + GFF27 0.40 -0.1 31,811 + GFF27 0.40 -1.4 31,811 + GFF27 0.40 +1.5 31,811 + GFF27 0.40 +0.3 31,811 + GFF27 0.40 -1.0 31,812 - GFF27 0.40 -0.3 31,812 - GFF27 0.40 -0.4 31,812 - GFF27 0.40 +0.3 31,812 - GFF27 0.40 -1.9 31,812 - GFF27 0.40 +0.5 31,883 + GFF27 0.48 -0.5 31,883 + GFF27 0.48 -0.0 32,249 + -0.4 32,249 + -0.2 32,249 + +0.6 32,249 + +1.1 32,250 - -0.0 32,250 - -0.8 32,250 - -0.8 32,250 - +1.8 32,250 - -0.7 32,264 + +1.1 32,264 + -0.2 32,264 + +0.2 32,265 - +0.2 32,379 - -0.3
Or see this region's nucleotide sequence