Strain Fitness in Variovorax sp. SCN45 around GFF2499

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2498 and GFF2499 overlap by 4 nucleotidesGFF2499 and GFF2500 overlap by 4 nucleotidesGFF2500 and GFF2501 are separated by 86 nucleotides GFF2498 - Glutamate N-acetyltransferase (EC 2.3.1.35) @ N-acetylglutamate synthase (EC 2.3.1.1), at 558,378 to 559,607 GFF2498 GFF2499 - ATP/GTP-binding protein, at 559,604 to 560,479 GFF2499 GFF2500 - Mutator MutT protein (7,8-dihydro-8-oxoguanine-triphosphatase) (EC 3.6.1.-), at 560,476 to 560,910 GFF2500 GFF2501 - O-antigen acetylase, at 560,997 to 562,961 GFF2501 Position (kb) 559 560 561Strain fitness (log2 ratio) -2 -1 0 1 2 3at 559.287 kb on + strand, within GFF2498at 559.287 kb on + strand, within GFF2498at 559.287 kb on + strand, within GFF2498at 559.288 kb on - strand, within GFF2498at 559.326 kb on + strand, within GFF2498at 559.326 kb on + strand, within GFF2498at 559.326 kb on + strand, within GFF2498at 559.327 kb on - strand, within GFF2498at 559.327 kb on - strand, within GFF2498at 559.327 kb on - strand, within GFF2498at 559.327 kb on - strand, within GFF2498at 559.327 kb on - strand, within GFF2498at 559.372 kb on - strand, within GFF2498at 559.462 kb on - strand, within GFF2498at 559.575 kb on + strandat 559.575 kb on + strandat 559.576 kb on - strandat 559.576 kb on - strandat 559.576 kb on - strandat 559.576 kb on - strandat 559.576 kb on - strandat 559.937 kb on + strand, within GFF2499at 559.937 kb on + strand, within GFF2499at 559.938 kb on - strand, within GFF2499at 560.081 kb on + strand, within GFF2499at 560.081 kb on + strand, within GFF2499at 560.081 kb on + strand, within GFF2499at 560.081 kb on + strand, within GFF2499at 560.081 kb on + strand, within GFF2499at 560.082 kb on - strand, within GFF2499at 560.082 kb on - strand, within GFF2499at 560.082 kb on - strand, within GFF2499at 560.082 kb on - strand, within GFF2499at 560.144 kb on + strand, within GFF2499at 560.145 kb on - strand, within GFF2499at 560.145 kb on - strand, within GFF2499at 560.145 kb on - strand, within GFF2499at 560.180 kb on + strand, within GFF2499at 560.181 kb on - strand, within GFF2499at 560.181 kb on - strand, within GFF2499at 560.201 kb on + strand, within GFF2499at 560.201 kb on + strand, within GFF2499at 560.202 kb on - strand, within GFF2499at 560.202 kb on - strand, within GFF2499at 560.294 kb on + strand, within GFF2499at 560.294 kb on + strand, within GFF2499at 560.295 kb on - strand, within GFF2499at 560.295 kb on - strand, within GFF2499at 560.295 kb on - strand, within GFF2499at 560.295 kb on - strand, within GFF2499at 560.315 kb on + strand, within GFF2499at 560.315 kb on + strand, within GFF2499at 560.316 kb on - strand, within GFF2499at 560.316 kb on - strand, within GFF2499at 560.438 kb on + strandat 560.439 kb on - strandat 560.456 kb on + strandat 560.457 kb on - strandat 560.457 kb on - strandat 560.575 kb on + strand, within GFF2500at 560.575 kb on + strand, within GFF2500at 560.576 kb on - strand, within GFF2500at 560.584 kb on + strand, within GFF2500at 560.585 kb on - strand, within GFF2500at 560.713 kb on + strand, within GFF2500at 560.908 kb on + strandat 560.908 kb on + strandat 560.909 kb on - strandat 560.909 kb on - strandat 560.909 kb on - strandat 560.973 kb on + strandat 561.009 kb on + strandat 561.009 kb on + strandat 561.009 kb on + strandat 561.009 kb on + strandat 561.010 kb on - strandat 561.010 kb on - strandat 561.031 kb on + strandat 561.032 kb on - strandat 561.063 kb on + strandat 561.063 kb on + strandat 561.063 kb on + strandat 561.064 kb on - strandat 561.064 kb on - strandat 561.178 kb on + strandat 561.195 kb on + strand, within GFF2501at 561.195 kb on + strand, within GFF2501at 561.195 kb on + strand, within GFF2501at 561.195 kb on + strand, within GFF2501at 561.195 kb on + strand, within GFF2501at 561.196 kb on - strand, within GFF2501at 561.217 kb on + strand, within GFF2501at 561.217 kb on + strand, within GFF2501at 561.217 kb on + strand, within GFF2501at 561.218 kb on - strand, within GFF2501at 561.267 kb on + strand, within GFF2501at 561.267 kb on + strand, within GFF2501at 561.268 kb on - strand, within GFF2501at 561.268 kb on - strand, within GFF2501at 561.268 kb on - strand, within GFF2501at 561.363 kb on + strand, within GFF2501at 561.432 kb on + strand, within GFF2501at 561.433 kb on - strand, within GFF2501at 561.433 kb on - strand, within GFF2501at 561.434 kb on + strand, within GFF2501at 561.434 kb on + strand, within GFF2501at 561.435 kb on - strand, within GFF2501

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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559,287 + GFF2498 0.74 +0.5
559,287 + GFF2498 0.74 -0.2
559,287 + GFF2498 0.74 +0.9
559,288 - GFF2498 0.74 +1.1
559,326 + GFF2498 0.77 -1.4
559,326 + GFF2498 0.77 +1.0
559,326 + GFF2498 0.77 +3.1
559,327 - GFF2498 0.77 -0.8
559,327 - GFF2498 0.77 -0.1
559,327 - GFF2498 0.77 -1.7
559,327 - GFF2498 0.77 -0.8
559,327 - GFF2498 0.77 -0.4
559,372 - GFF2498 0.81 -0.2
559,462 - GFF2498 0.88 -1.1
559,575 + -0.1
559,575 + -0.9
559,576 - -0.4
559,576 - +0.3
559,576 - -2.0
559,576 - -0.6
559,576 - +0.2
559,937 + GFF2499 0.38 +2.2
559,937 + GFF2499 0.38 +0.3
559,938 - GFF2499 0.38 +0.4
560,081 + GFF2499 0.54 +0.2
560,081 + GFF2499 0.54 +0.7
560,081 + GFF2499 0.54 -1.5
560,081 + GFF2499 0.54 +0.6
560,081 + GFF2499 0.54 -1.0
560,082 - GFF2499 0.55 +0.9
560,082 - GFF2499 0.55 +0.8
560,082 - GFF2499 0.55 -0.2
560,082 - GFF2499 0.55 +0.9
560,144 + GFF2499 0.62 -0.2
560,145 - GFF2499 0.62 +0.5
560,145 - GFF2499 0.62 -0.8
560,145 - GFF2499 0.62 -0.5
560,180 + GFF2499 0.66 -0.5
560,181 - GFF2499 0.66 +0.1
560,181 - GFF2499 0.66 -1.9
560,201 + GFF2499 0.68 -0.6
560,201 + GFF2499 0.68 -0.3
560,202 - GFF2499 0.68 +0.5
560,202 - GFF2499 0.68 +0.1
560,294 + GFF2499 0.79 -2.7
560,294 + GFF2499 0.79 +1.5
560,295 - GFF2499 0.79 -2.1
560,295 - GFF2499 0.79 +0.5
560,295 - GFF2499 0.79 -0.0
560,295 - GFF2499 0.79 -0.2
560,315 + GFF2499 0.81 -0.1
560,315 + GFF2499 0.81 -0.9
560,316 - GFF2499 0.81 +0.3
560,316 - GFF2499 0.81 -0.9
560,438 + -0.6
560,439 - +1.9
560,456 + -0.2
560,457 - -1.2
560,457 - -1.1
560,575 + GFF2500 0.23 -0.1
560,575 + GFF2500 0.23 -0.7
560,576 - GFF2500 0.23 -0.1
560,584 + GFF2500 0.25 -0.4
560,585 - GFF2500 0.25 -0.8
560,713 + GFF2500 0.54 +1.5
560,908 + +0.1
560,908 + +0.2
560,909 - -1.9
560,909 - -0.9
560,909 - +0.8
560,973 + -1.2
561,009 + -0.7
561,009 + +1.6
561,009 + -1.1
561,009 + +1.9
561,010 - +1.0
561,010 - -0.5
561,031 + +0.2
561,032 - +0.5
561,063 + -0.7
561,063 + +0.3
561,063 + -1.4
561,064 - -0.1
561,064 - +0.1
561,178 + -0.6
561,195 + GFF2501 0.10 +0.0
561,195 + GFF2501 0.10 +0.8
561,195 + GFF2501 0.10 -0.6
561,195 + GFF2501 0.10 -0.1
561,195 + GFF2501 0.10 -0.0
561,196 - GFF2501 0.10 +0.7
561,217 + GFF2501 0.11 -0.7
561,217 + GFF2501 0.11 -0.0
561,217 + GFF2501 0.11 +0.9
561,218 - GFF2501 0.11 -0.2
561,267 + GFF2501 0.14 +0.8
561,267 + GFF2501 0.14 -0.1
561,268 - GFF2501 0.14 -0.4
561,268 - GFF2501 0.14 +1.0
561,268 - GFF2501 0.14 +2.2
561,363 + GFF2501 0.19 +1.1
561,432 + GFF2501 0.22 +1.2
561,433 - GFF2501 0.22 -0.5
561,433 - GFF2501 0.22 -0.6
561,434 + GFF2501 0.22 -0.8
561,434 + GFF2501 0.22 +1.2
561,435 - GFF2501 0.22 +0.3

Or see this region's nucleotide sequence