Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2282 and GFF2283 overlap by 4 nucleotides GFF2283 and GFF2284 are separated by 106 nucleotides GFF2284 and GFF2285 are separated by 5 nucleotides
GFF2282 - Trans-feruloyl-CoA synthase (EC 6.2.1.34), at 336,777 to 338,630
GFF2282
GFF2283 - Enoyl-CoA hydratase (EC 4.2.1.17), at 338,627 to 339,400
GFF2283
GFF2284 - FIG00538168: hypothetical protein, at 339,507 to 340,385
GFF2284
GFF2285 - Transcriptional regulator, RpiR family, at 340,391 to 341,281
GFF2285
Position (kb) 339
340
341 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 338.551 kb on + strand at 338.552 kb on - strand at 338.787 kb on + strand, within GFF2283 at 338.787 kb on + strand, within GFF2283 at 338.868 kb on + strand, within GFF2283 at 338.868 kb on + strand, within GFF2283 at 338.868 kb on + strand, within GFF2283 at 338.868 kb on + strand, within GFF2283 at 338.869 kb on - strand, within GFF2283 at 338.907 kb on + strand, within GFF2283 at 338.908 kb on - strand, within GFF2283 at 339.022 kb on - strand, within GFF2283 at 339.022 kb on - strand, within GFF2283 at 339.022 kb on - strand, within GFF2283 at 339.429 kb on - strand at 339.583 kb on - strand at 339.583 kb on - strand at 339.583 kb on - strand at 339.595 kb on - strand, within GFF2284 at 339.595 kb on - strand, within GFF2284 at 339.615 kb on + strand, within GFF2284 at 339.615 kb on + strand, within GFF2284 at 339.615 kb on + strand, within GFF2284 at 339.615 kb on + strand, within GFF2284 at 339.615 kb on + strand, within GFF2284 at 339.616 kb on - strand, within GFF2284 at 339.616 kb on - strand, within GFF2284 at 339.616 kb on - strand, within GFF2284 at 339.616 kb on - strand, within GFF2284 at 339.660 kb on + strand, within GFF2284 at 339.660 kb on + strand, within GFF2284 at 339.661 kb on - strand, within GFF2284 at 339.661 kb on - strand, within GFF2284 at 339.661 kb on - strand, within GFF2284 at 339.663 kb on + strand, within GFF2284 at 339.897 kb on + strand, within GFF2284 at 339.898 kb on - strand, within GFF2284 at 339.898 kb on - strand, within GFF2284 at 339.906 kb on + strand, within GFF2284 at 339.906 kb on + strand, within GFF2284 at 339.906 kb on + strand, within GFF2284 at 339.930 kb on + strand, within GFF2284 at 339.930 kb on + strand, within GFF2284 at 339.930 kb on + strand, within GFF2284 at 339.930 kb on + strand, within GFF2284 at 339.931 kb on - strand, within GFF2284 at 339.931 kb on - strand, within GFF2284 at 340.030 kb on - strand, within GFF2284 at 340.185 kb on + strand, within GFF2284 at 340.186 kb on - strand, within GFF2284 at 340.186 kb on - strand, within GFF2284 at 340.209 kb on + strand, within GFF2284 at 340.210 kb on - strand, within GFF2284 at 340.210 kb on - strand, within GFF2284 at 340.532 kb on + strand, within GFF2285 at 340.551 kb on + strand, within GFF2285 at 340.551 kb on + strand, within GFF2285 at 340.552 kb on - strand, within GFF2285 at 340.671 kb on + strand, within GFF2285 at 340.671 kb on + strand, within GFF2285 at 340.671 kb on + strand, within GFF2285 at 340.672 kb on - strand, within GFF2285 at 341.094 kb on + strand, within GFF2285 at 341.094 kb on + strand, within GFF2285 at 341.095 kb on - strand, within GFF2285 at 341.095 kb on - strand, within GFF2285 at 341.253 kb on + strand at 341.253 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 338,551 + +1.1 338,552 - -1.4 338,787 + GFF2283 0.21 -1.4 338,787 + GFF2283 0.21 -1.1 338,868 + GFF2283 0.31 -0.2 338,868 + GFF2283 0.31 +0.2 338,868 + GFF2283 0.31 -0.1 338,868 + GFF2283 0.31 -0.2 338,869 - GFF2283 0.31 -2.0 338,907 + GFF2283 0.36 -0.0 338,908 - GFF2283 0.36 -0.8 339,022 - GFF2283 0.51 -0.5 339,022 - GFF2283 0.51 -1.7 339,022 - GFF2283 0.51 +0.8 339,429 - +0.5 339,583 - +0.1 339,583 - -0.6 339,583 - -0.9 339,595 - GFF2284 0.10 -0.5 339,595 - GFF2284 0.10 -1.3 339,615 + GFF2284 0.12 -0.3 339,615 + GFF2284 0.12 -1.6 339,615 + GFF2284 0.12 +1.6 339,615 + GFF2284 0.12 +0.3 339,615 + GFF2284 0.12 -0.3 339,616 - GFF2284 0.12 +0.8 339,616 - GFF2284 0.12 +0.8 339,616 - GFF2284 0.12 +0.1 339,616 - GFF2284 0.12 -1.0 339,660 + GFF2284 0.17 +0.2 339,660 + GFF2284 0.17 +0.0 339,661 - GFF2284 0.18 +0.6 339,661 - GFF2284 0.18 +1.9 339,661 - GFF2284 0.18 +0.2 339,663 + GFF2284 0.18 +0.0 339,897 + GFF2284 0.44 +1.1 339,898 - GFF2284 0.44 +1.7 339,898 - GFF2284 0.44 -0.8 339,906 + GFF2284 0.45 -1.5 339,906 + GFF2284 0.45 +0.2 339,906 + GFF2284 0.45 -0.4 339,930 + GFF2284 0.48 -1.2 339,930 + GFF2284 0.48 +0.4 339,930 + GFF2284 0.48 -1.8 339,930 + GFF2284 0.48 +0.9 339,931 - GFF2284 0.48 -2.6 339,931 - GFF2284 0.48 -1.8 340,030 - GFF2284 0.59 +0.9 340,185 + GFF2284 0.77 +0.6 340,186 - GFF2284 0.77 +1.0 340,186 - GFF2284 0.77 +0.6 340,209 + GFF2284 0.80 -0.4 340,210 - GFF2284 0.80 -0.3 340,210 - GFF2284 0.80 -0.6 340,532 + GFF2285 0.16 +0.2 340,551 + GFF2285 0.18 -0.1 340,551 + GFF2285 0.18 +1.2 340,552 - GFF2285 0.18 +0.6 340,671 + GFF2285 0.31 +0.8 340,671 + GFF2285 0.31 -0.2 340,671 + GFF2285 0.31 +0.9 340,672 - GFF2285 0.32 +1.5 341,094 + GFF2285 0.79 -0.2 341,094 + GFF2285 0.79 -0.9 341,095 - GFF2285 0.79 -2.9 341,095 - GFF2285 0.79 +0.4 341,253 + -0.3 341,253 + +0.5
Or see this region's nucleotide sequence