Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2085 and GFF2086 overlap by 4 nucleotides GFF2086 and GFF2087 overlap by 11 nucleotides GFF2087 and GFF2088 overlap by 4 nucleotides
GFF2085 - Acyl-CoA:1-acyl-sn-glycerol-3-phosphate acyltransferase (EC 2.3.1.51), at 132,910 to 133,638
GFF2085
GFF2086 - hypothetical protein, at 133,635 to 134,084
GFF2086
GFF2087 - Ser/Thr and Tyr protein phosphatase (dual specificity), at 134,074 to 135,423
GFF2087
GFF2088 - Putative lipase in cluster with Phosphatidate cytidylyltransferase, at 135,420 to 137,186
GFF2088
Position (kb) 134
135
136 Strain fitness (log2 ratio) -2
-1
0
1
2 at 133.367 kb on + strand, within GFF2085 at 133.367 kb on + strand, within GFF2085 at 133.367 kb on + strand, within GFF2085 at 133.466 kb on + strand, within GFF2085 at 133.466 kb on + strand, within GFF2085 at 133.467 kb on - strand, within GFF2085 at 133.891 kb on + strand, within GFF2086 at 133.891 kb on + strand, within GFF2086 at 133.892 kb on - strand, within GFF2086 at 134.642 kb on + strand, within GFF2087 at 134.642 kb on + strand, within GFF2087 at 134.642 kb on + strand, within GFF2087 at 134.642 kb on + strand, within GFF2087 at 134.643 kb on - strand, within GFF2087 at 134.747 kb on + strand, within GFF2087 at 134.748 kb on - strand, within GFF2087 at 134.785 kb on - strand, within GFF2087 at 135.006 kb on + strand, within GFF2087 at 135.006 kb on + strand, within GFF2087 at 135.007 kb on - strand, within GFF2087 at 135.218 kb on + strand, within GFF2087 at 135.218 kb on + strand, within GFF2087 at 135.219 kb on - strand, within GFF2087 at 135.240 kb on - strand, within GFF2087 at 135.268 kb on + strand, within GFF2087 at 135.269 kb on - strand, within GFF2087 at 135.341 kb on + strand at 135.341 kb on + strand at 135.341 kb on + strand at 135.350 kb on + strand at 135.350 kb on + strand at 135.350 kb on + strand at 135.350 kb on + strand at 135.351 kb on - strand at 135.351 kb on - strand at 135.351 kb on - strand at 135.351 kb on - strand at 135.351 kb on - strand at 135.625 kb on + strand, within GFF2088 at 135.626 kb on - strand, within GFF2088 at 135.710 kb on - strand, within GFF2088 at 135.920 kb on - strand, within GFF2088 at 135.920 kb on - strand, within GFF2088 at 135.999 kb on + strand, within GFF2088 at 135.999 kb on + strand, within GFF2088 at 135.999 kb on + strand, within GFF2088 at 136.000 kb on - strand, within GFF2088 at 136.057 kb on + strand, within GFF2088 at 136.058 kb on - strand, within GFF2088 at 136.111 kb on + strand, within GFF2088 at 136.117 kb on + strand, within GFF2088 at 136.117 kb on + strand, within GFF2088 at 136.117 kb on + strand, within GFF2088 at 136.219 kb on + strand, within GFF2088
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 133,367 + GFF2085 0.63 +0.2 133,367 + GFF2085 0.63 -2.0 133,367 + GFF2085 0.63 -2.1 133,466 + GFF2085 0.76 -1.3 133,466 + GFF2085 0.76 +1.0 133,467 - GFF2085 0.76 -1.0 133,891 + GFF2086 0.57 +0.4 133,891 + GFF2086 0.57 +0.6 133,892 - GFF2086 0.57 -0.6 134,642 + GFF2087 0.42 -0.6 134,642 + GFF2087 0.42 -0.2 134,642 + GFF2087 0.42 -1.7 134,642 + GFF2087 0.42 +0.4 134,643 - GFF2087 0.42 -2.2 134,747 + GFF2087 0.50 -0.9 134,748 - GFF2087 0.50 -0.9 134,785 - GFF2087 0.53 -0.1 135,006 + GFF2087 0.69 -1.0 135,006 + GFF2087 0.69 -0.9 135,007 - GFF2087 0.69 -1.1 135,218 + GFF2087 0.85 -0.0 135,218 + GFF2087 0.85 +0.8 135,219 - GFF2087 0.85 -0.9 135,240 - GFF2087 0.86 -0.0 135,268 + GFF2087 0.88 -1.4 135,269 - GFF2087 0.89 +0.1 135,341 + -0.9 135,341 + -0.2 135,341 + +0.2 135,350 + -0.2 135,350 + -0.5 135,350 + +2.0 135,350 + -0.2 135,351 - -0.1 135,351 - +0.2 135,351 - +0.3 135,351 - -0.1 135,351 - -0.0 135,625 + GFF2088 0.12 -0.2 135,626 - GFF2088 0.12 +0.5 135,710 - GFF2088 0.16 -0.0 135,920 - GFF2088 0.28 -0.5 135,920 - GFF2088 0.28 +0.1 135,999 + GFF2088 0.33 +0.4 135,999 + GFF2088 0.33 -0.4 135,999 + GFF2088 0.33 +1.5 136,000 - GFF2088 0.33 -0.6 136,057 + GFF2088 0.36 -0.2 136,058 - GFF2088 0.36 -0.6 136,111 + GFF2088 0.39 -2.4 136,117 + GFF2088 0.39 -0.1 136,117 + GFF2088 0.39 -0.3 136,117 + GFF2088 0.39 +0.7 136,219 + GFF2088 0.45 +0.2
Or see this region's nucleotide sequence