Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2032 and GFF2033 are separated by 7 nucleotides GFF2033 and GFF2034 are separated by 107 nucleotides
GFF2032 - Excinuclease ABC subunit A, dimeric form, at 75,064 to 80,907
GFF2032
GFF2033 - no description, at 80,915 to 81,871
GFF2033
GFF2034 - BUG/TctC family periplasmic protein, at 81,979 to 82,965
GFF2034
Position (kb)
80
81
82 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 80.089 kb on + strand, within GFF2032 at 80.090 kb on - strand, within GFF2032 at 80.090 kb on - strand, within GFF2032 at 80.108 kb on + strand, within GFF2032 at 80.164 kb on + strand, within GFF2032 at 80.164 kb on + strand, within GFF2032 at 80.164 kb on + strand, within GFF2032 at 80.164 kb on + strand, within GFF2032 at 80.165 kb on - strand, within GFF2032 at 80.165 kb on - strand, within GFF2032 at 80.165 kb on - strand, within GFF2032 at 80.165 kb on - strand, within GFF2032 at 80.165 kb on - strand, within GFF2032 at 80.165 kb on - strand, within GFF2032 at 80.165 kb on - strand, within GFF2032 at 80.165 kb on - strand, within GFF2032 at 80.165 kb on - strand, within GFF2032 at 80.165 kb on - strand, within GFF2032 at 80.512 kb on + strand at 80.513 kb on - strand at 80.513 kb on - strand at 80.641 kb on + strand at 80.641 kb on + strand at 80.641 kb on + strand at 80.641 kb on + strand at 80.641 kb on + strand at 80.641 kb on + strand at 80.641 kb on + strand at 80.641 kb on + strand at 80.641 kb on + strand at 80.642 kb on - strand at 80.642 kb on - strand at 80.642 kb on - strand at 81.119 kb on + strand, within GFF2033 at 81.120 kb on - strand, within GFF2033 at 81.323 kb on + strand, within GFF2033 at 81.323 kb on + strand, within GFF2033 at 81.323 kb on + strand, within GFF2033 at 81.323 kb on + strand, within GFF2033 at 81.324 kb on - strand, within GFF2033 at 81.324 kb on - strand, within GFF2033 at 81.324 kb on - strand, within GFF2033 at 81.837 kb on + strand at 81.837 kb on + strand at 81.837 kb on + strand at 81.837 kb on + strand at 81.912 kb on + strand at 81.940 kb on + strand at 81.940 kb on + strand at 81.940 kb on + strand at 81.940 kb on + strand at 81.940 kb on + strand at 81.940 kb on + strand at 81.940 kb on + strand at 81.940 kb on + strand at 81.940 kb on + strand at 81.940 kb on + strand at 81.941 kb on - strand at 81.941 kb on - strand at 81.941 kb on - strand at 81.941 kb on - strand at 82.246 kb on + strand, within GFF2034 at 82.246 kb on + strand, within GFF2034 at 82.444 kb on + strand, within GFF2034 at 82.444 kb on + strand, within GFF2034 at 82.444 kb on + strand, within GFF2034 at 82.444 kb on + strand, within GFF2034 at 82.445 kb on - strand, within GFF2034 at 82.445 kb on - strand, within GFF2034 at 82.445 kb on - strand, within GFF2034 at 82.445 kb on - strand, within GFF2034 at 82.445 kb on - strand, within GFF2034 at 82.445 kb on - strand, within GFF2034 at 82.445 kb on - strand, within GFF2034 at 82.445 kb on - strand, within GFF2034 at 82.603 kb on + strand, within GFF2034 at 82.603 kb on + strand, within GFF2034 at 82.603 kb on + strand, within GFF2034 at 82.621 kb on + strand, within GFF2034 at 82.622 kb on - strand, within GFF2034 at 82.622 kb on - strand, within GFF2034 at 82.622 kb on - strand, within GFF2034
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 80,089 + GFF2032 0.86 +0.1 80,090 - GFF2032 0.86 +0.8 80,090 - GFF2032 0.86 -1.4 80,108 + GFF2032 0.86 +1.0 80,164 + GFF2032 0.87 +0.3 80,164 + GFF2032 0.87 +2.0 80,164 + GFF2032 0.87 -3.0 80,164 + GFF2032 0.87 +0.0 80,165 - GFF2032 0.87 -0.3 80,165 - GFF2032 0.87 -1.0 80,165 - GFF2032 0.87 +0.0 80,165 - GFF2032 0.87 -2.0 80,165 - GFF2032 0.87 -0.4 80,165 - GFF2032 0.87 +0.3 80,165 - GFF2032 0.87 -1.4 80,165 - GFF2032 0.87 +3.0 80,165 - GFF2032 0.87 +0.4 80,165 - GFF2032 0.87 +0.3 80,512 + +0.3 80,513 - -0.5 80,513 - -0.2 80,641 + +0.1 80,641 + -0.3 80,641 + -0.2 80,641 + +0.8 80,641 + +0.1 80,641 + -0.6 80,641 + +0.4 80,641 + +0.2 80,641 + -0.6 80,642 - +2.5 80,642 - -0.7 80,642 - -1.0 81,119 + GFF2033 0.21 +1.0 81,120 - GFF2033 0.21 +0.1 81,323 + GFF2033 0.43 -0.6 81,323 + GFF2033 0.43 -0.5 81,323 + GFF2033 0.43 +0.0 81,323 + GFF2033 0.43 +0.1 81,324 - GFF2033 0.43 +0.4 81,324 - GFF2033 0.43 +0.2 81,324 - GFF2033 0.43 +0.1 81,837 + +0.6 81,837 + -2.4 81,837 + -0.5 81,837 + -1.1 81,912 + -0.6 81,940 + +0.0 81,940 + -0.2 81,940 + -0.8 81,940 + -1.0 81,940 + +0.2 81,940 + +0.3 81,940 + +0.1 81,940 + +2.0 81,940 + -1.7 81,940 + -0.3 81,941 - -0.4 81,941 - -0.6 81,941 - -1.2 81,941 - -0.6 82,246 + GFF2034 0.27 -0.6 82,246 + GFF2034 0.27 -0.3 82,444 + GFF2034 0.47 -0.3 82,444 + GFF2034 0.47 -0.6 82,444 + GFF2034 0.47 +0.4 82,444 + GFF2034 0.47 +0.8 82,445 - GFF2034 0.47 -0.6 82,445 - GFF2034 0.47 -0.0 82,445 - GFF2034 0.47 +0.5 82,445 - GFF2034 0.47 +0.0 82,445 - GFF2034 0.47 -0.3 82,445 - GFF2034 0.47 +0.4 82,445 - GFF2034 0.47 -0.4 82,445 - GFF2034 0.47 +0.2 82,603 + GFF2034 0.63 +0.2 82,603 + GFF2034 0.63 +1.2 82,603 + GFF2034 0.63 -1.2 82,621 + GFF2034 0.65 -0.1 82,622 - GFF2034 0.65 +0.1 82,622 - GFF2034 0.65 +1.2 82,622 - GFF2034 0.65 -0.0
Or see this region's nucleotide sequence