Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1460 and GFF1461 are separated by 2 nucleotides GFF1461 and GFF1462 overlap by 4 nucleotides GFF1462 and GFF1463 are separated by 51 nucleotides
GFF1460 - Enoyl-CoA hydratase/isomerase, at 51,846 to 52,592
GFF1460
GFF1461 - Enoyl-CoA hydratase (EC 4.2.1.17), at 52,595 to 53,401
GFF1461
GFF1462 - L-carnitine dehydratase/bile acid-inducible protein F, at 53,398 to 54,600
GFF1462
GFF1463 - Spermidine/putrescine import ABC transporter ATP-binding protein PotA (TC 3.A.1.11.1), at 54,652 to 55,713
GFF1463
Position (kb) 53
54
55 Strain fitness (log2 ratio) -2
-1
0
1
2 at 52.405 kb on + strand, within GFF1460 at 52.421 kb on - strand, within GFF1460 at 52.421 kb on - strand, within GFF1460 at 52.653 kb on + strand at 52.653 kb on + strand at 52.791 kb on + strand, within GFF1461 at 52.792 kb on - strand, within GFF1461 at 52.792 kb on - strand, within GFF1461 at 52.792 kb on - strand, within GFF1461 at 52.947 kb on + strand, within GFF1461 at 52.948 kb on - strand, within GFF1461 at 52.979 kb on + strand, within GFF1461 at 53.130 kb on + strand, within GFF1461 at 53.130 kb on + strand, within GFF1461 at 53.131 kb on - strand, within GFF1461 at 53.131 kb on - strand, within GFF1461 at 53.131 kb on - strand, within GFF1461 at 53.510 kb on + strand at 53.645 kb on + strand, within GFF1462 at 53.777 kb on + strand, within GFF1462 at 53.777 kb on + strand, within GFF1462 at 53.778 kb on - strand, within GFF1462 at 53.837 kb on + strand, within GFF1462 at 53.837 kb on + strand, within GFF1462 at 53.838 kb on - strand, within GFF1462 at 53.838 kb on - strand, within GFF1462 at 53.838 kb on - strand, within GFF1462 at 53.838 kb on - strand, within GFF1462 at 53.838 kb on - strand, within GFF1462 at 53.932 kb on + strand, within GFF1462 at 53.952 kb on - strand, within GFF1462 at 54.270 kb on - strand, within GFF1462 at 54.776 kb on + strand, within GFF1463 at 54.797 kb on + strand, within GFF1463 at 54.798 kb on - strand, within GFF1463 at 55.037 kb on + strand, within GFF1463 at 55.038 kb on - strand, within GFF1463 at 55.038 kb on - strand, within GFF1463 at 55.038 kb on - strand, within GFF1463 at 55.451 kb on + strand, within GFF1463 at 55.451 kb on + strand, within GFF1463 at 55.451 kb on + strand, within GFF1463 at 55.556 kb on - strand, within GFF1463
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 52,405 + GFF1460 0.75 -0.9 52,421 - GFF1460 0.77 +0.5 52,421 - GFF1460 0.77 +0.9 52,653 + +0.7 52,653 + -0.1 52,791 + GFF1461 0.24 +0.6 52,792 - GFF1461 0.24 -0.4 52,792 - GFF1461 0.24 -1.1 52,792 - GFF1461 0.24 -2.4 52,947 + GFF1461 0.44 +0.2 52,948 - GFF1461 0.44 -1.6 52,979 + GFF1461 0.48 +0.1 53,130 + GFF1461 0.66 -1.5 53,130 + GFF1461 0.66 -0.6 53,131 - GFF1461 0.66 +0.1 53,131 - GFF1461 0.66 -0.6 53,131 - GFF1461 0.66 -0.3 53,510 + -0.7 53,645 + GFF1462 0.21 -0.4 53,777 + GFF1462 0.32 +1.2 53,777 + GFF1462 0.32 +0.3 53,778 - GFF1462 0.32 +0.5 53,837 + GFF1462 0.36 -1.0 53,837 + GFF1462 0.36 +1.7 53,838 - GFF1462 0.37 +0.8 53,838 - GFF1462 0.37 +1.7 53,838 - GFF1462 0.37 +2.7 53,838 - GFF1462 0.37 -1.3 53,838 - GFF1462 0.37 -0.4 53,932 + GFF1462 0.44 +0.7 53,952 - GFF1462 0.46 +0.2 54,270 - GFF1462 0.72 +0.3 54,776 + GFF1463 0.12 +0.0 54,797 + GFF1463 0.14 +0.3 54,798 - GFF1463 0.14 +1.6 55,037 + GFF1463 0.36 -0.1 55,038 - GFF1463 0.36 +0.0 55,038 - GFF1463 0.36 +1.6 55,038 - GFF1463 0.36 -1.0 55,451 + GFF1463 0.75 +0.8 55,451 + GFF1463 0.75 -1.1 55,451 + GFF1463 0.75 -1.1 55,556 - GFF1463 0.85 +1.1
Or see this region's nucleotide sequence