Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1309 and GFF1310 overlap by 4 nucleotides GFF1310 and GFF1311 are separated by 160 nucleotides GFF1311 and GFF1312 are separated by 135 nucleotides
GFF1309 - Phosphoglucomutase (EC 5.4.2.2), at 638,886 to 640,541
GFF1309
GFF1310 - hypothetical protein, at 640,538 to 641,215
GFF1310
GFF1311 - no description, at 641,376 to 641,711
GFF1311
GFF1312 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 641,847 to 643,247
GFF1312
Position (kb) 640
641
642 Strain fitness (log2 ratio) -2
-1
0
1
2
3
4 at 639.556 kb on + strand, within GFF1309 at 639.557 kb on - strand, within GFF1309 at 639.781 kb on + strand, within GFF1309 at 639.782 kb on - strand, within GFF1309 at 639.782 kb on - strand, within GFF1309 at 639.782 kb on - strand, within GFF1309 at 639.806 kb on - strand, within GFF1309 at 639.806 kb on - strand, within GFF1309 at 639.901 kb on + strand, within GFF1309 at 639.901 kb on + strand, within GFF1309 at 639.901 kb on + strand, within GFF1309 at 639.901 kb on + strand, within GFF1309 at 639.902 kb on - strand, within GFF1309 at 639.902 kb on - strand, within GFF1309 at 639.902 kb on - strand, within GFF1309 at 639.902 kb on - strand, within GFF1309 at 639.902 kb on - strand, within GFF1309 at 639.902 kb on - strand, within GFF1309 at 639.902 kb on - strand, within GFF1309 at 639.902 kb on - strand, within GFF1309 at 639.902 kb on - strand, within GFF1309 at 639.913 kb on + strand, within GFF1309 at 639.913 kb on + strand, within GFF1309 at 639.914 kb on - strand, within GFF1309 at 640.063 kb on + strand, within GFF1309 at 640.063 kb on + strand, within GFF1309 at 640.063 kb on + strand, within GFF1309 at 640.063 kb on + strand, within GFF1309 at 640.063 kb on + strand, within GFF1309 at 640.064 kb on - strand, within GFF1309 at 640.064 kb on - strand, within GFF1309 at 640.064 kb on - strand, within GFF1309 at 640.064 kb on - strand, within GFF1309 at 640.064 kb on - strand, within GFF1309 at 640.064 kb on - strand, within GFF1309 at 640.148 kb on - strand, within GFF1309 at 640.186 kb on + strand, within GFF1309 at 640.186 kb on + strand, within GFF1309 at 640.187 kb on - strand, within GFF1309 at 640.187 kb on - strand, within GFF1309 at 640.187 kb on - strand, within GFF1309 at 640.456 kb on + strand at 640.456 kb on + strand at 640.456 kb on + strand at 640.456 kb on + strand at 640.456 kb on + strand at 640.456 kb on + strand at 640.457 kb on - strand at 640.457 kb on - strand at 640.457 kb on - strand at 640.457 kb on - strand at 640.457 kb on - strand at 640.459 kb on + strand at 640.459 kb on + strand at 640.460 kb on - strand at 640.605 kb on + strand at 640.605 kb on + strand at 640.605 kb on + strand at 640.635 kb on + strand, within GFF1310 at 640.635 kb on + strand, within GFF1310 at 640.635 kb on + strand, within GFF1310 at 640.635 kb on + strand, within GFF1310 at 640.635 kb on + strand, within GFF1310 at 640.635 kb on + strand, within GFF1310 at 640.636 kb on - strand, within GFF1310 at 640.636 kb on - strand, within GFF1310 at 640.636 kb on - strand, within GFF1310 at 640.636 kb on - strand, within GFF1310 at 640.636 kb on - strand, within GFF1310 at 640.636 kb on - strand, within GFF1310 at 640.728 kb on + strand, within GFF1310 at 640.728 kb on + strand, within GFF1310 at 640.728 kb on + strand, within GFF1310 at 640.729 kb on - strand, within GFF1310 at 640.729 kb on - strand, within GFF1310 at 640.729 kb on - strand, within GFF1310 at 640.729 kb on - strand, within GFF1310 at 640.729 kb on - strand, within GFF1310 at 640.785 kb on + strand, within GFF1310 at 640.785 kb on + strand, within GFF1310 at 640.785 kb on + strand, within GFF1310 at 640.785 kb on + strand, within GFF1310 at 640.786 kb on - strand, within GFF1310 at 640.786 kb on - strand, within GFF1310 at 640.786 kb on - strand, within GFF1310 at 640.786 kb on - strand, within GFF1310 at 640.871 kb on + strand, within GFF1310 at 640.871 kb on + strand, within GFF1310 at 640.871 kb on + strand, within GFF1310 at 640.872 kb on - strand, within GFF1310 at 640.872 kb on - strand, within GFF1310 at 640.872 kb on - strand, within GFF1310 at 640.872 kb on - strand, within GFF1310 at 640.872 kb on - strand, within GFF1310 at 640.872 kb on - strand, within GFF1310 at 641.020 kb on - strand, within GFF1310 at 641.040 kb on + strand, within GFF1310 at 641.041 kb on - strand, within GFF1310 at 641.041 kb on - strand, within GFF1310 at 641.049 kb on + strand, within GFF1310 at 641.050 kb on - strand, within GFF1310 at 641.050 kb on - strand, within GFF1310 at 641.050 kb on - strand, within GFF1310 at 641.261 kb on + strand at 641.261 kb on + strand at 641.261 kb on + strand at 641.261 kb on + strand at 641.261 kb on + strand at 641.262 kb on - strand at 641.262 kb on - strand at 641.262 kb on - strand at 641.262 kb on - strand at 641.262 kb on - strand at 641.262 kb on - strand at 641.262 kb on - strand at 641.262 kb on - strand at 641.262 kb on - strand at 641.300 kb on + strand at 641.300 kb on + strand at 641.300 kb on + strand at 641.300 kb on + strand at 641.301 kb on - strand at 641.301 kb on - strand at 641.301 kb on - strand at 641.301 kb on - strand at 641.301 kb on - strand at 641.301 kb on - strand at 641.327 kb on + strand at 641.327 kb on + strand at 641.327 kb on + strand at 641.327 kb on + strand at 641.328 kb on - strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.381 kb on + strand at 641.382 kb on - strand at 641.382 kb on - strand at 641.382 kb on - strand at 641.382 kb on - strand at 641.382 kb on - strand at 641.382 kb on - strand at 641.382 kb on - strand at 641.382 kb on - strand at 641.382 kb on - strand at 641.398 kb on - strand at 641.700 kb on + strand at 641.700 kb on + strand at 641.700 kb on + strand at 641.700 kb on + strand at 641.700 kb on + strand at 641.700 kb on + strand at 641.700 kb on + strand at 641.700 kb on + strand at 641.700 kb on + strand at 641.700 kb on + strand at 641.701 kb on - strand at 641.701 kb on - strand at 641.701 kb on - strand at 641.701 kb on - strand at 641.701 kb on - strand at 641.701 kb on - strand at 641.701 kb on - strand at 641.701 kb on - strand at 641.701 kb on - strand at 641.701 kb on - strand at 641.701 kb on - strand at 641.701 kb on - strand at 641.710 kb on - strand at 641.817 kb on + strand at 641.817 kb on + strand at 641.817 kb on + strand at 641.817 kb on + strand at 641.817 kb on + strand at 641.817 kb on + strand at 641.817 kb on + strand at 641.817 kb on + strand at 641.817 kb on + strand at 641.817 kb on + strand at 641.817 kb on + strand at 641.817 kb on + strand at 641.817 kb on + strand at 641.817 kb on + strand at 641.817 kb on + strand at 641.817 kb on + strand at 641.817 kb on + strand at 641.817 kb on + strand at 641.817 kb on + strand at 641.817 kb on + strand at 641.817 kb on + strand at 641.818 kb on - strand at 641.818 kb on - strand at 641.818 kb on - strand at 641.818 kb on - strand at 641.818 kb on - strand at 641.818 kb on - strand at 641.818 kb on - strand at 641.818 kb on - strand at 641.886 kb on + strand at 641.886 kb on + strand at 641.886 kb on + strand at 641.887 kb on - strand at 641.887 kb on - strand at 641.979 kb on + strand at 641.979 kb on + strand at 641.980 kb on - strand at 641.980 kb on - strand at 641.980 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 639,556 + GFF1309 0.40 +0.2 639,557 - GFF1309 0.41 +0.7 639,781 + GFF1309 0.54 -1.9 639,782 - GFF1309 0.54 -1.1 639,782 - GFF1309 0.54 -0.4 639,782 - GFF1309 0.54 -1.4 639,806 - GFF1309 0.56 -0.7 639,806 - GFF1309 0.56 +0.1 639,901 + GFF1309 0.61 +1.7 639,901 + GFF1309 0.61 -1.7 639,901 + GFF1309 0.61 -0.2 639,901 + GFF1309 0.61 -1.8 639,902 - GFF1309 0.61 -0.3 639,902 - GFF1309 0.61 +0.5 639,902 - GFF1309 0.61 +0.1 639,902 - GFF1309 0.61 +0.1 639,902 - GFF1309 0.61 -0.5 639,902 - GFF1309 0.61 -0.1 639,902 - GFF1309 0.61 +1.5 639,902 - GFF1309 0.61 +0.2 639,902 - GFF1309 0.61 -0.4 639,913 + GFF1309 0.62 -0.4 639,913 + GFF1309 0.62 +1.1 639,914 - GFF1309 0.62 -0.1 640,063 + GFF1309 0.71 +0.6 640,063 + GFF1309 0.71 +0.3 640,063 + GFF1309 0.71 -0.8 640,063 + GFF1309 0.71 +1.0 640,063 + GFF1309 0.71 +0.8 640,064 - GFF1309 0.71 +2.2 640,064 - GFF1309 0.71 -0.0 640,064 - GFF1309 0.71 +0.0 640,064 - GFF1309 0.71 +0.6 640,064 - GFF1309 0.71 +0.2 640,064 - GFF1309 0.71 -0.1 640,148 - GFF1309 0.76 +0.4 640,186 + GFF1309 0.79 -0.3 640,186 + GFF1309 0.79 -0.3 640,187 - GFF1309 0.79 -1.9 640,187 - GFF1309 0.79 -0.1 640,187 - GFF1309 0.79 +1.1 640,456 + -0.1 640,456 + -0.3 640,456 + +1.5 640,456 + +1.2 640,456 + +0.7 640,456 + +1.5 640,457 - +0.4 640,457 - +0.0 640,457 - -1.4 640,457 - +0.6 640,457 - +0.5 640,459 + +0.3 640,459 + -0.9 640,460 - -0.2 640,605 + +0.0 640,605 + -0.3 640,605 + -0.3 640,635 + GFF1310 0.14 -0.5 640,635 + GFF1310 0.14 -0.1 640,635 + GFF1310 0.14 -0.6 640,635 + GFF1310 0.14 -0.2 640,635 + GFF1310 0.14 -1.5 640,635 + GFF1310 0.14 -0.2 640,636 - GFF1310 0.14 +0.2 640,636 - GFF1310 0.14 +0.3 640,636 - GFF1310 0.14 -2.3 640,636 - GFF1310 0.14 +0.4 640,636 - GFF1310 0.14 +0.9 640,636 - GFF1310 0.14 +0.6 640,728 + GFF1310 0.28 -0.2 640,728 + GFF1310 0.28 +0.8 640,728 + GFF1310 0.28 +0.2 640,729 - GFF1310 0.28 +0.4 640,729 - GFF1310 0.28 +0.2 640,729 - GFF1310 0.28 +0.3 640,729 - GFF1310 0.28 -0.1 640,729 - GFF1310 0.28 +0.7 640,785 + GFF1310 0.36 +0.2 640,785 + GFF1310 0.36 -0.8 640,785 + GFF1310 0.36 -0.1 640,785 + GFF1310 0.36 +0.1 640,786 - GFF1310 0.37 -0.2 640,786 - GFF1310 0.37 +0.0 640,786 - GFF1310 0.37 +0.2 640,786 - GFF1310 0.37 -0.1 640,871 + GFF1310 0.49 +0.2 640,871 + GFF1310 0.49 -0.2 640,871 + GFF1310 0.49 -0.5 640,872 - GFF1310 0.49 -0.1 640,872 - GFF1310 0.49 +0.4 640,872 - GFF1310 0.49 +0.1 640,872 - GFF1310 0.49 -0.1 640,872 - GFF1310 0.49 -1.1 640,872 - GFF1310 0.49 -2.6 641,020 - GFF1310 0.71 -0.7 641,040 + GFF1310 0.74 +0.4 641,041 - GFF1310 0.74 -1.1 641,041 - GFF1310 0.74 -1.3 641,049 + GFF1310 0.75 -0.5 641,050 - GFF1310 0.76 +0.3 641,050 - GFF1310 0.76 +0.3 641,050 - GFF1310 0.76 -1.2 641,261 + -0.8 641,261 + -0.6 641,261 + +0.3 641,261 + -0.8 641,261 + +0.5 641,262 - -0.7 641,262 - -0.1 641,262 - -1.7 641,262 - -1.1 641,262 - +0.4 641,262 - +0.4 641,262 - -1.5 641,262 - -0.2 641,262 - +0.2 641,300 + -1.3 641,300 + +0.6 641,300 + -0.0 641,300 + -1.5 641,301 - +1.2 641,301 - +1.7 641,301 - -0.1 641,301 - -0.2 641,301 - -0.3 641,301 - -1.5 641,327 + -0.7 641,327 + +0.4 641,327 + +1.3 641,327 + +0.5 641,328 - +0.1 641,381 + +0.4 641,381 + -1.9 641,381 + -0.2 641,381 + +0.9 641,381 + +0.7 641,381 + -0.0 641,381 + -1.4 641,381 + +0.1 641,381 + -0.6 641,381 + -0.4 641,381 + -0.8 641,381 + -1.0 641,381 + -0.4 641,381 + -0.1 641,382 - +0.3 641,382 - +0.3 641,382 - -2.3 641,382 - +0.8 641,382 - -0.1 641,382 - +0.1 641,382 - -0.3 641,382 - -0.9 641,382 - -1.4 641,398 - +0.2 641,700 + +3.8 641,700 + +0.8 641,700 + +1.0 641,700 + -0.1 641,700 + -0.5 641,700 + +0.6 641,700 + +0.2 641,700 + +0.2 641,700 + -0.8 641,700 + +0.2 641,701 - +0.3 641,701 - +0.7 641,701 - -0.8 641,701 - -0.9 641,701 - -1.9 641,701 - +0.0 641,701 - -1.3 641,701 - -0.7 641,701 - +0.0 641,701 - -0.4 641,701 - +0.2 641,701 - +0.2 641,710 - +1.3 641,817 + -1.1 641,817 + -0.4 641,817 + -0.5 641,817 + -0.1 641,817 + -0.4 641,817 + -0.5 641,817 + -1.7 641,817 + -0.4 641,817 + -0.2 641,817 + -0.9 641,817 + +0.2 641,817 + -0.8 641,817 + -0.2 641,817 + -0.9 641,817 + +0.1 641,817 + +0.3 641,817 + +0.3 641,817 + +0.0 641,817 + -1.5 641,817 + -0.1 641,817 + -0.9 641,818 - -1.7 641,818 - -0.2 641,818 - -0.7 641,818 - +0.5 641,818 - +0.1 641,818 - -1.2 641,818 - -1.6 641,818 - +1.7 641,886 + -0.9 641,886 + -0.2 641,886 + +2.0 641,887 - +0.1 641,887 - -0.9 641,979 + -0.1 641,979 + +1.1 641,980 - -0.1 641,980 - +0.6 641,980 - +0.3
Or see this region's nucleotide sequence