Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1277 and GFF1278 are separated by 52 nucleotides GFF1278 and GFF1279 overlap by 4 nucleotides GFF1279 and GFF1280 are separated by 13 nucleotides
GFF1277 - Alkanesulfonates-binding protein, at 607,159 to 608,130
GFF1277
GFF1278 - no description, at 608,183 to 608,305
GFF1278
GFF1279 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 608,302 to 609,135
GFF1279
GFF1280 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 609,149 to 609,856
GFF1280
Position (kb)
608
609 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 607.289 kb on + strand, within GFF1277 at 607.290 kb on - strand, within GFF1277 at 607.290 kb on - strand, within GFF1277 at 607.469 kb on + strand, within GFF1277 at 607.469 kb on + strand, within GFF1277 at 607.470 kb on - strand, within GFF1277 at 607.470 kb on - strand, within GFF1277 at 607.587 kb on - strand, within GFF1277 at 607.587 kb on - strand, within GFF1277 at 607.619 kb on + strand, within GFF1277 at 607.619 kb on + strand, within GFF1277 at 607.619 kb on + strand, within GFF1277 at 607.620 kb on - strand, within GFF1277 at 607.620 kb on - strand, within GFF1277 at 607.620 kb on - strand, within GFF1277 at 607.679 kb on + strand, within GFF1277 at 607.680 kb on - strand, within GFF1277 at 607.680 kb on - strand, within GFF1277 at 607.680 kb on - strand, within GFF1277 at 607.680 kb on - strand, within GFF1277 at 607.680 kb on - strand, within GFF1277 at 607.680 kb on - strand, within GFF1277 at 607.680 kb on - strand, within GFF1277 at 607.692 kb on - strand, within GFF1277 at 607.692 kb on - strand, within GFF1277 at 607.805 kb on + strand, within GFF1277 at 607.806 kb on - strand, within GFF1277 at 607.806 kb on - strand, within GFF1277 at 608.061 kb on + strand at 608.155 kb on + strand at 608.155 kb on + strand at 608.155 kb on + strand at 608.155 kb on + strand at 608.156 kb on - strand at 608.156 kb on - strand at 608.156 kb on - strand at 608.221 kb on + strand, within GFF1278 at 608.221 kb on + strand, within GFF1278 at 608.221 kb on + strand, within GFF1278 at 608.221 kb on + strand, within GFF1278 at 608.221 kb on + strand, within GFF1278 at 608.221 kb on + strand, within GFF1278 at 608.222 kb on - strand, within GFF1278 at 608.222 kb on - strand, within GFF1278 at 608.222 kb on - strand, within GFF1278 at 608.222 kb on - strand, within GFF1278 at 608.222 kb on - strand, within GFF1278 at 608.266 kb on + strand, within GFF1278 at 608.266 kb on + strand, within GFF1278 at 608.266 kb on + strand, within GFF1278 at 608.284 kb on + strand, within GFF1278 at 608.284 kb on + strand, within GFF1278 at 608.284 kb on + strand, within GFF1278 at 608.285 kb on - strand, within GFF1278 at 608.453 kb on - strand, within GFF1279 at 608.659 kb on + strand, within GFF1279 at 608.660 kb on - strand, within GFF1279 at 608.660 kb on - strand, within GFF1279 at 608.783 kb on - strand, within GFF1279 at 608.783 kb on - strand, within GFF1279 at 608.806 kb on + strand, within GFF1279 at 608.807 kb on - strand, within GFF1279 at 608.807 kb on - strand, within GFF1279 at 608.807 kb on - strand, within GFF1279 at 608.942 kb on + strand, within GFF1279 at 608.953 kb on + strand, within GFF1279 at 608.953 kb on + strand, within GFF1279 at 608.953 kb on + strand, within GFF1279 at 608.954 kb on - strand, within GFF1279 at 609.052 kb on + strand at 609.052 kb on + strand at 609.052 kb on + strand at 609.053 kb on - strand at 609.053 kb on - strand at 609.053 kb on - strand at 609.053 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 607,289 + GFF1277 0.13 -0.1 607,290 - GFF1277 0.13 +0.3 607,290 - GFF1277 0.13 -1.5 607,469 + GFF1277 0.32 -0.2 607,469 + GFF1277 0.32 -1.4 607,470 - GFF1277 0.32 -0.3 607,470 - GFF1277 0.32 +0.3 607,587 - GFF1277 0.44 -0.5 607,587 - GFF1277 0.44 -0.0 607,619 + GFF1277 0.47 -0.1 607,619 + GFF1277 0.47 +0.3 607,619 + GFF1277 0.47 -1.5 607,620 - GFF1277 0.47 -1.0 607,620 - GFF1277 0.47 -0.6 607,620 - GFF1277 0.47 -1.2 607,679 + GFF1277 0.53 -0.6 607,680 - GFF1277 0.54 +2.4 607,680 - GFF1277 0.54 -0.0 607,680 - GFF1277 0.54 -0.5 607,680 - GFF1277 0.54 -0.1 607,680 - GFF1277 0.54 -0.5 607,680 - GFF1277 0.54 -0.8 607,680 - GFF1277 0.54 -0.1 607,692 - GFF1277 0.55 -1.6 607,692 - GFF1277 0.55 -0.1 607,805 + GFF1277 0.66 +0.2 607,806 - GFF1277 0.67 +0.3 607,806 - GFF1277 0.67 -0.7 608,061 + -0.0 608,155 + -0.9 608,155 + -0.9 608,155 + +0.3 608,155 + -0.3 608,156 - -0.3 608,156 - -0.5 608,156 - +0.2 608,221 + GFF1278 0.31 +2.0 608,221 + GFF1278 0.31 -0.1 608,221 + GFF1278 0.31 +1.4 608,221 + GFF1278 0.31 -0.2 608,221 + GFF1278 0.31 -0.2 608,221 + GFF1278 0.31 -0.0 608,222 - GFF1278 0.32 -1.5 608,222 - GFF1278 0.32 -0.8 608,222 - GFF1278 0.32 -0.4 608,222 - GFF1278 0.32 -0.0 608,222 - GFF1278 0.32 +0.0 608,266 + GFF1278 0.67 -0.7 608,266 + GFF1278 0.67 -0.1 608,266 + GFF1278 0.67 +0.0 608,284 + GFF1278 0.82 +0.6 608,284 + GFF1278 0.82 +0.9 608,284 + GFF1278 0.82 -0.6 608,285 - GFF1278 0.83 +0.6 608,453 - GFF1279 0.18 +1.0 608,659 + GFF1279 0.43 +0.8 608,660 - GFF1279 0.43 -0.9 608,660 - GFF1279 0.43 -2.7 608,783 - GFF1279 0.58 +1.2 608,783 - GFF1279 0.58 -0.2 608,806 + GFF1279 0.60 +1.5 608,807 - GFF1279 0.61 -0.2 608,807 - GFF1279 0.61 -0.8 608,807 - GFF1279 0.61 +0.5 608,942 + GFF1279 0.77 -0.5 608,953 + GFF1279 0.78 -0.3 608,953 + GFF1279 0.78 +0.1 608,953 + GFF1279 0.78 -2.3 608,954 - GFF1279 0.78 +0.0 609,052 + +0.5 609,052 + -1.2 609,052 + -1.9 609,053 - +0.6 609,053 - -0.3 609,053 - -0.7 609,053 - -0.6
Or see this region's nucleotide sequence