Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1273 and GFF1274 are separated by 141 nucleotides GFF1274 and GFF1275 are separated by 4 nucleotides GFF1275 and GFF1276 are separated by 72 nucleotides
GFF1273 - no description, at 603,833 to 604,066
GFF1273
GFF1274 - Glutamate racemase (EC 5.1.1.3), at 604,208 to 604,903
GFF1274
GFF1275 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 604,908 to 605,840
GFF1275
GFF1276 - Sarcosine oxidase, at 605,913 to 607,136
GFF1276
Position (kb) 604
605
606 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 603.954 kb on + strand, within GFF1273 at 603.954 kb on + strand, within GFF1273 at 603.954 kb on + strand, within GFF1273 at 603.954 kb on + strand, within GFF1273 at 603.954 kb on + strand, within GFF1273 at 603.954 kb on + strand, within GFF1273 at 603.954 kb on + strand, within GFF1273 at 603.954 kb on + strand, within GFF1273 at 603.955 kb on - strand, within GFF1273 at 603.955 kb on - strand, within GFF1273 at 604.069 kb on + strand at 604.069 kb on + strand at 604.069 kb on + strand at 604.069 kb on + strand at 604.069 kb on + strand at 604.069 kb on + strand at 604.069 kb on + strand at 604.069 kb on + strand at 604.069 kb on + strand at 604.069 kb on + strand at 604.070 kb on - strand at 604.070 kb on - strand at 604.070 kb on - strand at 604.070 kb on - strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.147 kb on + strand at 604.148 kb on - strand at 604.148 kb on - strand at 604.148 kb on - strand at 604.148 kb on - strand at 604.148 kb on - strand at 604.148 kb on - strand at 604.148 kb on - strand at 604.148 kb on - strand at 604.148 kb on - strand at 604.154 kb on + strand at 604.154 kb on + strand at 604.154 kb on + strand at 604.154 kb on + strand at 604.154 kb on + strand at 604.154 kb on + strand at 604.154 kb on + strand at 604.155 kb on - strand at 604.155 kb on - strand at 604.155 kb on - strand at 604.155 kb on - strand at 604.155 kb on - strand at 604.155 kb on - strand at 604.209 kb on + strand at 604.209 kb on + strand at 604.210 kb on - strand at 604.301 kb on + strand, within GFF1274 at 604.301 kb on + strand, within GFF1274 at 604.301 kb on + strand, within GFF1274 at 604.301 kb on + strand, within GFF1274 at 604.301 kb on + strand, within GFF1274 at 604.301 kb on + strand, within GFF1274 at 604.302 kb on - strand, within GFF1274 at 604.419 kb on + strand, within GFF1274 at 604.728 kb on + strand, within GFF1274 at 604.729 kb on + strand, within GFF1274 at 604.729 kb on + strand, within GFF1274 at 604.729 kb on + strand, within GFF1274 at 604.729 kb on + strand, within GFF1274 at 604.729 kb on + strand, within GFF1274 at 604.729 kb on + strand, within GFF1274 at 604.729 kb on + strand, within GFF1274 at 604.729 kb on + strand, within GFF1274 at 604.729 kb on + strand, within GFF1274 at 604.730 kb on - strand, within GFF1274 at 604.730 kb on - strand, within GFF1274 at 604.730 kb on - strand, within GFF1274 at 604.730 kb on - strand, within GFF1274 at 604.730 kb on - strand, within GFF1274 at 604.730 kb on - strand, within GFF1274 at 604.730 kb on - strand, within GFF1274 at 604.939 kb on + strand at 604.939 kb on + strand at 604.939 kb on + strand at 604.939 kb on + strand at 604.939 kb on + strand at 604.939 kb on + strand at 604.939 kb on + strand at 604.939 kb on + strand at 604.940 kb on - strand at 604.940 kb on - strand at 604.957 kb on + strand at 604.957 kb on + strand at 604.957 kb on + strand at 604.957 kb on + strand at 604.957 kb on + strand at 604.957 kb on + strand at 604.957 kb on + strand at 604.958 kb on - strand at 604.958 kb on - strand at 604.958 kb on - strand at 604.958 kb on - strand at 604.958 kb on - strand at 604.958 kb on - strand at 604.958 kb on - strand at 604.958 kb on - strand at 604.958 kb on - strand at 605.068 kb on + strand, within GFF1275 at 605.068 kb on + strand, within GFF1275 at 605.068 kb on + strand, within GFF1275 at 605.068 kb on + strand, within GFF1275 at 605.068 kb on + strand, within GFF1275 at 605.068 kb on + strand, within GFF1275 at 605.069 kb on - strand, within GFF1275 at 605.069 kb on - strand, within GFF1275 at 605.069 kb on - strand, within GFF1275 at 605.069 kb on - strand, within GFF1275 at 605.069 kb on - strand, within GFF1275 at 605.069 kb on - strand, within GFF1275 at 605.069 kb on - strand, within GFF1275 at 605.069 kb on - strand, within GFF1275 at 605.069 kb on - strand, within GFF1275 at 605.069 kb on - strand, within GFF1275 at 605.399 kb on - strand, within GFF1275 at 605.466 kb on - strand, within GFF1275 at 605.605 kb on + strand, within GFF1275 at 605.605 kb on + strand, within GFF1275 at 605.605 kb on + strand, within GFF1275 at 605.605 kb on + strand, within GFF1275 at 605.606 kb on - strand, within GFF1275 at 605.606 kb on - strand, within GFF1275 at 605.606 kb on - strand, within GFF1275 at 605.606 kb on - strand, within GFF1275 at 605.636 kb on - strand, within GFF1275 at 605.800 kb on + strand at 605.801 kb on - strand at 605.985 kb on + strand at 605.985 kb on + strand at 605.985 kb on + strand at 605.985 kb on + strand at 605.985 kb on + strand at 605.985 kb on + strand at 605.985 kb on + strand at 605.985 kb on + strand at 605.985 kb on + strand at 605.986 kb on - strand at 605.986 kb on - strand at 605.986 kb on - strand at 605.986 kb on - strand at 605.986 kb on - strand at 606.118 kb on + strand, within GFF1276 at 606.118 kb on + strand, within GFF1276 at 606.118 kb on + strand, within GFF1276 at 606.118 kb on + strand, within GFF1276 at 606.118 kb on + strand, within GFF1276 at 606.118 kb on + strand, within GFF1276 at 606.118 kb on + strand, within GFF1276 at 606.118 kb on + strand, within GFF1276 at 606.119 kb on - strand, within GFF1276 at 606.119 kb on - strand, within GFF1276 at 606.119 kb on - strand, within GFF1276 at 606.119 kb on - strand, within GFF1276 at 606.119 kb on - strand, within GFF1276 at 606.119 kb on - strand, within GFF1276 at 606.119 kb on - strand, within GFF1276 at 606.119 kb on - strand, within GFF1276 at 606.119 kb on - strand, within GFF1276 at 606.119 kb on - strand, within GFF1276 at 606.119 kb on - strand, within GFF1276 at 606.188 kb on - strand, within GFF1276 at 606.188 kb on - strand, within GFF1276 at 606.188 kb on - strand, within GFF1276 at 606.188 kb on - strand, within GFF1276 at 606.188 kb on - strand, within GFF1276 at 606.253 kb on + strand, within GFF1276 at 606.253 kb on + strand, within GFF1276 at 606.253 kb on + strand, within GFF1276 at 606.253 kb on + strand, within GFF1276 at 606.253 kb on + strand, within GFF1276 at 606.253 kb on + strand, within GFF1276 at 606.253 kb on + strand, within GFF1276 at 606.253 kb on + strand, within GFF1276 at 606.254 kb on - strand, within GFF1276 at 606.254 kb on - strand, within GFF1276 at 606.254 kb on - strand, within GFF1276 at 606.254 kb on - strand, within GFF1276 at 606.254 kb on - strand, within GFF1276 at 606.254 kb on - strand, within GFF1276 at 606.254 kb on - strand, within GFF1276 at 606.309 kb on + strand, within GFF1276 at 606.310 kb on + strand, within GFF1276 at 606.310 kb on + strand, within GFF1276 at 606.310 kb on + strand, within GFF1276 at 606.310 kb on + strand, within GFF1276 at 606.310 kb on + strand, within GFF1276 at 606.311 kb on - strand, within GFF1276 at 606.311 kb on - strand, within GFF1276 at 606.311 kb on - strand, within GFF1276 at 606.311 kb on - strand, within GFF1276 at 606.311 kb on - strand, within GFF1276 at 606.311 kb on - strand, within GFF1276 at 606.316 kb on + strand, within GFF1276 at 606.316 kb on + strand, within GFF1276 at 606.316 kb on + strand, within GFF1276 at 606.316 kb on + strand, within GFF1276 at 606.316 kb on + strand, within GFF1276 at 606.316 kb on + strand, within GFF1276 at 606.316 kb on + strand, within GFF1276 at 606.316 kb on + strand, within GFF1276 at 606.317 kb on - strand, within GFF1276 at 606.317 kb on - strand, within GFF1276 at 606.317 kb on - strand, within GFF1276 at 606.317 kb on - strand, within GFF1276 at 606.692 kb on - strand, within GFF1276
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 603,954 + GFF1273 0.52 -1.2 603,954 + GFF1273 0.52 +0.3 603,954 + GFF1273 0.52 +1.4 603,954 + GFF1273 0.52 -0.3 603,954 + GFF1273 0.52 +0.2 603,954 + GFF1273 0.52 -1.3 603,954 + GFF1273 0.52 -0.6 603,954 + GFF1273 0.52 +2.5 603,955 - GFF1273 0.52 -0.1 603,955 - GFF1273 0.52 +0.4 604,069 + +1.5 604,069 + -0.5 604,069 + +0.3 604,069 + -0.6 604,069 + -1.1 604,069 + -0.2 604,069 + +0.1 604,069 + -1.3 604,069 + -0.4 604,069 + +0.1 604,070 - +1.5 604,070 - -0.4 604,070 - -1.0 604,070 - -0.2 604,147 + -0.2 604,147 + -1.8 604,147 + +0.1 604,147 + -0.9 604,147 + +0.1 604,147 + +0.7 604,147 + -1.5 604,147 + -1.0 604,147 + -0.5 604,147 + +0.2 604,147 + -0.5 604,147 + +0.7 604,148 - -0.1 604,148 - -1.5 604,148 - -0.2 604,148 - -1.7 604,148 - -2.3 604,148 - -0.8 604,148 - -0.3 604,148 - +1.3 604,148 - -1.3 604,154 + -1.2 604,154 + +0.7 604,154 + -0.2 604,154 + +0.5 604,154 + -0.7 604,154 + -0.4 604,154 + -0.4 604,155 - -1.5 604,155 - +0.1 604,155 - -0.8 604,155 - +0.9 604,155 - +0.2 604,155 - -2.9 604,209 + +0.3 604,209 + +2.2 604,210 - -0.7 604,301 + GFF1274 0.13 -0.2 604,301 + GFF1274 0.13 -0.6 604,301 + GFF1274 0.13 +0.3 604,301 + GFF1274 0.13 -1.0 604,301 + GFF1274 0.13 +0.4 604,301 + GFF1274 0.13 -0.0 604,302 - GFF1274 0.14 +0.5 604,419 + GFF1274 0.30 -0.8 604,728 + GFF1274 0.75 +0.5 604,729 + GFF1274 0.75 -0.9 604,729 + GFF1274 0.75 -1.5 604,729 + GFF1274 0.75 -0.7 604,729 + GFF1274 0.75 -1.9 604,729 + GFF1274 0.75 +0.5 604,729 + GFF1274 0.75 +1.2 604,729 + GFF1274 0.75 +0.0 604,729 + GFF1274 0.75 -0.1 604,729 + GFF1274 0.75 -0.1 604,730 - GFF1274 0.75 -1.2 604,730 - GFF1274 0.75 -3.5 604,730 - GFF1274 0.75 -0.6 604,730 - GFF1274 0.75 +0.1 604,730 - GFF1274 0.75 -0.2 604,730 - GFF1274 0.75 -0.3 604,730 - GFF1274 0.75 -1.1 604,939 + -0.1 604,939 + +1.8 604,939 + -0.9 604,939 + -1.0 604,939 + +0.8 604,939 + -0.3 604,939 + -0.5 604,939 + +0.9 604,940 - +0.5 604,940 - +1.1 604,957 + +0.0 604,957 + -0.9 604,957 + +0.0 604,957 + -2.6 604,957 + -1.3 604,957 + -1.0 604,957 + +0.1 604,958 - -0.1 604,958 - -2.4 604,958 - -1.8 604,958 - -0.6 604,958 - +0.6 604,958 - -1.1 604,958 - -0.3 604,958 - -0.5 604,958 - +0.4 605,068 + GFF1275 0.17 -0.1 605,068 + GFF1275 0.17 -0.4 605,068 + GFF1275 0.17 -0.4 605,068 + GFF1275 0.17 +0.4 605,068 + GFF1275 0.17 +0.3 605,068 + GFF1275 0.17 -1.1 605,069 - GFF1275 0.17 -0.4 605,069 - GFF1275 0.17 -0.8 605,069 - GFF1275 0.17 -1.4 605,069 - GFF1275 0.17 -0.5 605,069 - GFF1275 0.17 -0.6 605,069 - GFF1275 0.17 -2.1 605,069 - GFF1275 0.17 -0.2 605,069 - GFF1275 0.17 +0.8 605,069 - GFF1275 0.17 -1.2 605,069 - GFF1275 0.17 -0.1 605,399 - GFF1275 0.53 +0.0 605,466 - GFF1275 0.60 -0.5 605,605 + GFF1275 0.75 -0.8 605,605 + GFF1275 0.75 +0.5 605,605 + GFF1275 0.75 -1.1 605,605 + GFF1275 0.75 +0.0 605,606 - GFF1275 0.75 +0.5 605,606 - GFF1275 0.75 -0.2 605,606 - GFF1275 0.75 -0.5 605,606 - GFF1275 0.75 -0.2 605,636 - GFF1275 0.78 -1.9 605,800 + -0.6 605,801 - +0.9 605,985 + +0.4 605,985 + -0.8 605,985 + +0.9 605,985 + -0.3 605,985 + +1.1 605,985 + -1.1 605,985 + +2.1 605,985 + -0.4 605,985 + -0.9 605,986 - +0.7 605,986 - +0.1 605,986 - -0.8 605,986 - -0.8 605,986 - -1.7 606,118 + GFF1276 0.17 +0.8 606,118 + GFF1276 0.17 +0.5 606,118 + GFF1276 0.17 +0.2 606,118 + GFF1276 0.17 +0.1 606,118 + GFF1276 0.17 -1.1 606,118 + GFF1276 0.17 -1.3 606,118 + GFF1276 0.17 +0.2 606,118 + GFF1276 0.17 +0.5 606,119 - GFF1276 0.17 -1.8 606,119 - GFF1276 0.17 -0.7 606,119 - GFF1276 0.17 -0.3 606,119 - GFF1276 0.17 -0.2 606,119 - GFF1276 0.17 -0.7 606,119 - GFF1276 0.17 -0.6 606,119 - GFF1276 0.17 +1.5 606,119 - GFF1276 0.17 -0.2 606,119 - GFF1276 0.17 -0.7 606,119 - GFF1276 0.17 -1.2 606,119 - GFF1276 0.17 -0.4 606,188 - GFF1276 0.22 +0.7 606,188 - GFF1276 0.22 +0.5 606,188 - GFF1276 0.22 -0.1 606,188 - GFF1276 0.22 -0.3 606,188 - GFF1276 0.22 -0.0 606,253 + GFF1276 0.28 -0.2 606,253 + GFF1276 0.28 -1.1 606,253 + GFF1276 0.28 +0.0 606,253 + GFF1276 0.28 -0.0 606,253 + GFF1276 0.28 +0.6 606,253 + GFF1276 0.28 +1.2 606,253 + GFF1276 0.28 -1.7 606,253 + GFF1276 0.28 -0.1 606,254 - GFF1276 0.28 +0.5 606,254 - GFF1276 0.28 -0.6 606,254 - GFF1276 0.28 +0.1 606,254 - GFF1276 0.28 -0.8 606,254 - GFF1276 0.28 +1.0 606,254 - GFF1276 0.28 +1.0 606,254 - GFF1276 0.28 -0.9 606,309 + GFF1276 0.32 -1.4 606,310 + GFF1276 0.32 -0.5 606,310 + GFF1276 0.32 -0.8 606,310 + GFF1276 0.32 -0.8 606,310 + GFF1276 0.32 -0.6 606,310 + GFF1276 0.32 -0.1 606,311 - GFF1276 0.33 +0.7 606,311 - GFF1276 0.33 +1.5 606,311 - GFF1276 0.33 -0.2 606,311 - GFF1276 0.33 +0.6 606,311 - GFF1276 0.33 +1.0 606,311 - GFF1276 0.33 -1.2 606,316 + GFF1276 0.33 -1.0 606,316 + GFF1276 0.33 +1.1 606,316 + GFF1276 0.33 -1.1 606,316 + GFF1276 0.33 -0.4 606,316 + GFF1276 0.33 -0.2 606,316 + GFF1276 0.33 -0.8 606,316 + GFF1276 0.33 +0.7 606,316 + GFF1276 0.33 +0.9 606,317 - GFF1276 0.33 -0.7 606,317 - GFF1276 0.33 -0.7 606,317 - GFF1276 0.33 -1.3 606,317 - GFF1276 0.33 -0.5 606,692 - GFF1276 0.64 +1.1
Or see this region's nucleotide sequence