Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF124 and GFF125 overlap by 4 nucleotides GFF125 and GFF126 are separated by 32 nucleotides GFF126 and GFF127 are separated by 23 nucleotides
GFF124 - Beta-ureidopropionase (EC 3.5.1.6), at 152,650 to 153,930
GFF124
GFF125 - Dihydroorotase (EC 3.5.2.3), at 153,927 to 154,979
GFF125
GFF126 - Repressor homolog, at 155,012 to 155,920
GFF126
GFF127 - Orotate phosphoribosyltransferase (EC 2.4.2.10), at 155,944 to 156,627
GFF127
Position (kb) 153
154
155 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 153.067 kb on + strand, within GFF124 at 153.067 kb on + strand, within GFF124 at 153.067 kb on + strand, within GFF124 at 153.068 kb on - strand, within GFF124 at 153.068 kb on - strand, within GFF124 at 153.068 kb on - strand, within GFF124 at 153.068 kb on - strand, within GFF124 at 153.233 kb on - strand, within GFF124 at 153.233 kb on - strand, within GFF124 at 153.590 kb on - strand, within GFF124 at 154.108 kb on - strand, within GFF125 at 154.596 kb on + strand, within GFF125 at 154.596 kb on + strand, within GFF125 at 154.596 kb on + strand, within GFF125 at 154.770 kb on + strand, within GFF125 at 154.771 kb on - strand, within GFF125 at 154.975 kb on + strand at 154.976 kb on - strand at 154.977 kb on + strand at 154.978 kb on - strand at 154.978 kb on - strand at 154.978 kb on - strand at 154.978 kb on - strand at 155.238 kb on + strand, within GFF126 at 155.239 kb on - strand, within GFF126 at 155.258 kb on + strand, within GFF126 at 155.258 kb on + strand, within GFF126 at 155.259 kb on - strand, within GFF126 at 155.367 kb on - strand, within GFF126 at 155.864 kb on + strand at 155.864 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL remove 153,067 + GFF124 0.33 -1.5 153,067 + GFF124 0.33 +1.2 153,067 + GFF124 0.33 -0.9 153,068 - GFF124 0.33 -0.4 153,068 - GFF124 0.33 +0.7 153,068 - GFF124 0.33 +0.1 153,068 - GFF124 0.33 -0.4 153,233 - GFF124 0.46 -1.6 153,233 - GFF124 0.46 -0.3 153,590 - GFF124 0.73 -0.7 154,108 - GFF125 0.17 +1.3 154,596 + GFF125 0.64 -0.5 154,596 + GFF125 0.64 -3.0 154,596 + GFF125 0.64 -1.5 154,770 + GFF125 0.80 +1.8 154,771 - GFF125 0.80 +0.5 154,975 + -0.5 154,976 - -0.3 154,977 + -0.1 154,978 - -1.3 154,978 - -0.9 154,978 - -0.6 154,978 - +0.9 155,238 + GFF126 0.25 +0.9 155,239 - GFF126 0.25 -0.9 155,258 + GFF126 0.27 +0.2 155,258 + GFF126 0.27 +0.7 155,259 - GFF126 0.27 +0.2 155,367 - GFF126 0.39 -0.5 155,864 + -1.9 155,864 + +0.2
Or see this region's nucleotide sequence