Strain Fitness in Variovorax sp. SCN45 around GFF102

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF101 and GFF102 are separated by 184 nucleotidesGFF102 and GFF103 are separated by 17 nucleotides GFF101 - Phosphate-binding DING protein (related to PstS), at 126,440 to 127,729 GFF101 GFF102 - General secretion pathway protein F, at 127,914 to 129,119 GFF102 GFF103 - General secretion pathway protein E, at 129,137 to 130,489 GFF103 Position (kb) 127 128 129 130Strain fitness (log2 ratio) -3 -2 -1 0 1at 127.111 kb on - strand, within GFF101at 127.152 kb on + strand, within GFF101at 127.153 kb on - strand, within GFF101at 127.294 kb on - strand, within GFF101at 127.294 kb on - strand, within GFF101at 127.401 kb on + strand, within GFF101at 127.401 kb on + strand, within GFF101at 127.401 kb on + strand, within GFF101at 127.402 kb on - strand, within GFF101at 127.402 kb on - strand, within GFF101at 127.402 kb on - strand, within GFF101at 127.563 kb on + strand, within GFF101at 127.563 kb on + strand, within GFF101at 127.563 kb on + strand, within GFF101at 127.569 kb on + strand, within GFF101at 127.569 kb on + strand, within GFF101at 127.569 kb on + strand, within GFF101at 127.570 kb on - strand, within GFF101at 127.570 kb on - strand, within GFF101at 127.570 kb on - strand, within GFF101at 127.850 kb on - strandat 127.894 kb on - strandat 128.561 kb on - strand, within GFF102at 128.615 kb on - strand, within GFF102at 128.632 kb on - strand, within GFF102at 128.734 kb on + strand, within GFF102at 129.989 kb on + strand, within GFF103at 129.990 kb on - strand, within GFF103at 129.990 kb on - strand, within GFF103

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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127,111 - GFF101 0.52 -0.0
127,152 + GFF101 0.55 -1.9
127,153 - GFF101 0.55 -0.4
127,294 - GFF101 0.66 +0.5
127,294 - GFF101 0.66 -1.2
127,401 + GFF101 0.74 +0.2
127,401 + GFF101 0.74 -1.6
127,401 + GFF101 0.74 +0.5
127,402 - GFF101 0.75 -0.5
127,402 - GFF101 0.75 +0.2
127,402 - GFF101 0.75 -1.1
127,563 + GFF101 0.87 +0.3
127,563 + GFF101 0.87 -0.5
127,563 + GFF101 0.87 -0.4
127,569 + GFF101 0.88 -3.3
127,569 + GFF101 0.88 -1.1
127,569 + GFF101 0.88 -0.8
127,570 - GFF101 0.88 -0.2
127,570 - GFF101 0.88 -0.5
127,570 - GFF101 0.88 +0.5
127,850 - +1.0
127,894 - +0.2
128,561 - GFF102 0.54 -0.8
128,615 - GFF102 0.58 -0.3
128,632 - GFF102 0.60 -0.9
128,734 + GFF102 0.68 -0.9
129,989 + GFF103 0.63 -1.5
129,990 - GFF103 0.63 +1.5
129,990 - GFF103 0.63 -0.0

Or see this region's nucleotide sequence