Strain Fitness in Variovorax sp. SCN45 around GFF992

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF990 and GFF991 are separated by 152 nucleotidesGFF991 and GFF992 are separated by 21 nucleotidesGFF992 and GFF993 are separated by 4 nucleotidesGFF993 and GFF994 overlap by 8 nucleotides GFF990 - LSU rRNA pseudouridine(2605) synthase (EC 5.4.99.22), at 308,595 to 310,394 GFF990 GFF991 - Nucleoside diphosphate kinase (EC 2.7.4.6), at 310,547 to 310,972 GFF991 GFF992 - 23S rRNA (adenine(2503)-C(2))-methyltransferase @ tRNA (adenine(37)-C(2))-methyltransferase (EC 2.1.1.192), at 310,994 to 312,142 GFF992 GFF993 - Type IV pilus biogenesis protein PilF, at 312,147 to 313,001 GFF993 GFF994 - Cytoskeleton protein RodZ, at 312,994 to 313,959 GFF994 Position (kb) 310 311 312 313Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 310.266 kb on + strandat 310.266 kb on + strandat 310.266 kb on + strandat 310.266 kb on + strandat 310.266 kb on + strandat 310.266 kb on + strandat 310.267 kb on - strandat 310.267 kb on - strandat 310.267 kb on - strandat 310.267 kb on - strandat 310.267 kb on - strandat 310.480 kb on + strandat 310.480 kb on + strandat 310.961 kb on + strandat 310.961 kb on + strandat 310.961 kb on + strandat 310.962 kb on - strandat 310.978 kb on + strandat 310.978 kb on + strandat 310.978 kb on + strandat 310.978 kb on + strandat 310.978 kb on + strandat 310.978 kb on + strandat 310.978 kb on + strandat 310.979 kb on - strandat 310.979 kb on - strandat 310.979 kb on - strandat 310.979 kb on - strandat 311.274 kb on + strand, within GFF992at 311.274 kb on + strand, within GFF992at 311.274 kb on + strand, within GFF992at 311.275 kb on - strand, within GFF992at 311.275 kb on - strand, within GFF992at 311.275 kb on - strand, within GFF992at 311.275 kb on - strand, within GFF992at 311.465 kb on + strand, within GFF992at 311.507 kb on + strand, within GFF992at 311.507 kb on + strand, within GFF992at 311.507 kb on + strand, within GFF992at 311.507 kb on + strand, within GFF992at 311.555 kb on + strand, within GFF992at 311.708 kb on + strand, within GFF992at 311.708 kb on + strand, within GFF992at 311.708 kb on + strand, within GFF992at 311.708 kb on + strand, within GFF992at 311.709 kb on - strand, within GFF992at 311.744 kb on + strand, within GFF992at 311.744 kb on + strand, within GFF992at 311.744 kb on + strand, within GFF992at 311.783 kb on + strand, within GFF992at 311.783 kb on + strand, within GFF992at 311.783 kb on + strand, within GFF992at 311.784 kb on - strand, within GFF992at 311.784 kb on - strand, within GFF992at 311.784 kb on - strand, within GFF992at 311.784 kb on - strand, within GFF992at 312.373 kb on - strand, within GFF993at 312.453 kb on + strand, within GFF993at 312.453 kb on + strand, within GFF993at 312.453 kb on + strand, within GFF993at 312.453 kb on + strand, within GFF993at 312.453 kb on + strand, within GFF993at 312.453 kb on + strand, within GFF993at 312.453 kb on + strand, within GFF993at 312.453 kb on + strand, within GFF993at 312.454 kb on - strand, within GFF993at 312.454 kb on - strand, within GFF993at 312.454 kb on - strand, within GFF993at 312.454 kb on - strand, within GFF993at 312.454 kb on - strand, within GFF993at 312.474 kb on + strand, within GFF993at 312.474 kb on + strand, within GFF993at 312.474 kb on + strand, within GFF993at 312.474 kb on + strand, within GFF993at 312.474 kb on + strand, within GFF993at 312.474 kb on + strand, within GFF993at 312.474 kb on + strand, within GFF993at 312.475 kb on - strand, within GFF993at 312.475 kb on - strand, within GFF993at 312.475 kb on - strand, within GFF993at 312.475 kb on - strand, within GFF993at 312.475 kb on - strand, within GFF993at 312.475 kb on - strand, within GFF993at 312.475 kb on - strand, within GFF993at 312.475 kb on - strand, within GFF993at 312.475 kb on - strand, within GFF993at 312.564 kb on + strand, within GFF993at 312.564 kb on + strand, within GFF993at 312.564 kb on + strand, within GFF993at 312.564 kb on + strand, within GFF993at 312.564 kb on + strand, within GFF993at 312.564 kb on + strand, within GFF993at 312.564 kb on + strand, within GFF993at 312.564 kb on + strand, within GFF993at 312.564 kb on + strand, within GFF993at 312.564 kb on + strand, within GFF993at 312.564 kb on + strand, within GFF993at 312.564 kb on + strand, within GFF993at 312.564 kb on + strand, within GFF993at 312.564 kb on + strand, within GFF993at 312.564 kb on + strand, within GFF993at 312.564 kb on + strand, within GFF993at 312.565 kb on - strand, within GFF993at 312.565 kb on - strand, within GFF993at 312.565 kb on - strand, within GFF993at 312.565 kb on - strand, within GFF993at 312.565 kb on - strand, within GFF993at 312.565 kb on - strand, within GFF993at 312.565 kb on - strand, within GFF993at 312.565 kb on - strand, within GFF993at 312.565 kb on - strand, within GFF993at 312.565 kb on - strand, within GFF993at 312.565 kb on - strand, within GFF993at 312.565 kb on - strand, within GFF993at 312.565 kb on - strand, within GFF993at 312.565 kb on - strand, within GFF993at 312.565 kb on - strand, within GFF993at 312.565 kb on - strand, within GFF993at 312.642 kb on + strand, within GFF993at 312.642 kb on + strand, within GFF993at 312.642 kb on + strand, within GFF993at 312.643 kb on - strand, within GFF993at 312.735 kb on + strand, within GFF993at 312.735 kb on + strand, within GFF993at 312.735 kb on + strand, within GFF993at 312.736 kb on - strand, within GFF993at 312.736 kb on - strand, within GFF993at 312.736 kb on - strand, within GFF993at 312.736 kb on - strand, within GFF993at 312.822 kb on + strand, within GFF993at 312.822 kb on + strand, within GFF993at 312.822 kb on + strand, within GFF993at 312.822 kb on + strand, within GFF993at 312.822 kb on + strand, within GFF993at 312.822 kb on + strand, within GFF993at 312.822 kb on + strand, within GFF993at 312.822 kb on + strand, within GFF993at 312.822 kb on + strand, within GFF993at 312.822 kb on + strand, within GFF993at 312.823 kb on - strand, within GFF993at 312.823 kb on - strand, within GFF993at 312.823 kb on - strand, within GFF993at 312.823 kb on - strand, within GFF993at 312.823 kb on - strand, within GFF993at 312.823 kb on - strand, within GFF993at 312.975 kb on + strandat 312.975 kb on + strandat 312.975 kb on + strandat 312.976 kb on - strandat 312.976 kb on - strandat 312.976 kb on - strandat 312.976 kb on - strandat 312.976 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 10 mg/mL
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310,266 + +1.0
310,266 + -0.1
310,266 + +0.3
310,266 + +0.4
310,266 + -0.7
310,266 + +0.1
310,267 - +0.4
310,267 - -1.8
310,267 - +1.0
310,267 - +0.1
310,267 - -0.2
310,480 + -2.4
310,480 + -1.6
310,961 + -0.8
310,961 + -1.4
310,961 + -1.4
310,962 - -1.8
310,978 + -0.5
310,978 + -1.9
310,978 + -0.3
310,978 + -0.8
310,978 + -0.1
310,978 + +0.8
310,978 + -0.9
310,979 - -0.7
310,979 - -1.7
310,979 - -0.3
310,979 - +0.3
311,274 + GFF992 0.24 -0.5
311,274 + GFF992 0.24 -0.2
311,274 + GFF992 0.24 -0.8
311,275 - GFF992 0.24 -1.2
311,275 - GFF992 0.24 -0.5
311,275 - GFF992 0.24 -0.5
311,275 - GFF992 0.24 -0.4
311,465 + GFF992 0.41 -0.1
311,507 + GFF992 0.45 -0.9
311,507 + GFF992 0.45 -0.4
311,507 + GFF992 0.45 -1.6
311,507 + GFF992 0.45 -0.8
311,555 + GFF992 0.49 -1.2
311,708 + GFF992 0.62 +0.7
311,708 + GFF992 0.62 -1.1
311,708 + GFF992 0.62 -0.3
311,708 + GFF992 0.62 -0.4
311,709 - GFF992 0.62 -0.6
311,744 + GFF992 0.65 -0.5
311,744 + GFF992 0.65 -0.1
311,744 + GFF992 0.65 +0.3
311,783 + GFF992 0.69 -0.1
311,783 + GFF992 0.69 -1.1
311,783 + GFF992 0.69 -0.8
311,784 - GFF992 0.69 +0.1
311,784 - GFF992 0.69 -1.1
311,784 - GFF992 0.69 -1.1
311,784 - GFF992 0.69 -1.7
312,373 - GFF993 0.26 -1.8
312,453 + GFF993 0.36 -0.2
312,453 + GFF993 0.36 -0.4
312,453 + GFF993 0.36 -1.6
312,453 + GFF993 0.36 -0.3
312,453 + GFF993 0.36 +0.2
312,453 + GFF993 0.36 -0.9
312,453 + GFF993 0.36 -1.6
312,453 + GFF993 0.36 -1.3
312,454 - GFF993 0.36 -1.6
312,454 - GFF993 0.36 -0.5
312,454 - GFF993 0.36 -0.1
312,454 - GFF993 0.36 +0.9
312,454 - GFF993 0.36 -1.8
312,474 + GFF993 0.38 -1.4
312,474 + GFF993 0.38 -1.5
312,474 + GFF993 0.38 -0.4
312,474 + GFF993 0.38 -0.6
312,474 + GFF993 0.38 -1.0
312,474 + GFF993 0.38 -2.1
312,474 + GFF993 0.38 -0.8
312,475 - GFF993 0.38 +1.0
312,475 - GFF993 0.38 -1.3
312,475 - GFF993 0.38 -1.2
312,475 - GFF993 0.38 -2.2
312,475 - GFF993 0.38 -1.1
312,475 - GFF993 0.38 -0.8
312,475 - GFF993 0.38 -0.6
312,475 - GFF993 0.38 -0.5
312,475 - GFF993 0.38 -2.0
312,564 + GFF993 0.49 -1.1
312,564 + GFF993 0.49 -0.5
312,564 + GFF993 0.49 -2.1
312,564 + GFF993 0.49 -0.6
312,564 + GFF993 0.49 -1.5
312,564 + GFF993 0.49 -0.7
312,564 + GFF993 0.49 -0.1
312,564 + GFF993 0.49 -0.5
312,564 + GFF993 0.49 +0.5
312,564 + GFF993 0.49 -0.1
312,564 + GFF993 0.49 +0.5
312,564 + GFF993 0.49 -1.1
312,564 + GFF993 0.49 -1.3
312,564 + GFF993 0.49 -0.9
312,564 + GFF993 0.49 -0.3
312,564 + GFF993 0.49 -1.3
312,565 - GFF993 0.49 -1.5
312,565 - GFF993 0.49 +0.6
312,565 - GFF993 0.49 -1.7
312,565 - GFF993 0.49 -1.0
312,565 - GFF993 0.49 -1.3
312,565 - GFF993 0.49 -1.6
312,565 - GFF993 0.49 -1.5
312,565 - GFF993 0.49 -0.9
312,565 - GFF993 0.49 -1.3
312,565 - GFF993 0.49 -0.9
312,565 - GFF993 0.49 -0.8
312,565 - GFF993 0.49 -0.3
312,565 - GFF993 0.49 -0.5
312,565 - GFF993 0.49 +1.9
312,565 - GFF993 0.49 -2.6
312,565 - GFF993 0.49 -0.9
312,642 + GFF993 0.58 -1.7
312,642 + GFF993 0.58 -0.8
312,642 + GFF993 0.58 -1.1
312,643 - GFF993 0.58 -0.5
312,735 + GFF993 0.69 -1.8
312,735 + GFF993 0.69 -1.6
312,735 + GFF993 0.69 -1.3
312,736 - GFF993 0.69 -0.3
312,736 - GFF993 0.69 -2.0
312,736 - GFF993 0.69 -1.3
312,736 - GFF993 0.69 -2.6
312,822 + GFF993 0.79 +0.3
312,822 + GFF993 0.79 -2.0
312,822 + GFF993 0.79 -0.9
312,822 + GFF993 0.79 -1.1
312,822 + GFF993 0.79 -0.5
312,822 + GFF993 0.79 -1.6
312,822 + GFF993 0.79 -1.2
312,822 + GFF993 0.79 -1.2
312,822 + GFF993 0.79 -1.6
312,822 + GFF993 0.79 -1.4
312,823 - GFF993 0.79 +0.2
312,823 - GFF993 0.79 -1.7
312,823 - GFF993 0.79 -1.4
312,823 - GFF993 0.79 -0.8
312,823 - GFF993 0.79 -1.1
312,823 - GFF993 0.79 -0.8
312,975 + +0.0
312,975 + -0.6
312,975 + -0.1
312,976 - -1.8
312,976 - -1.5
312,976 - -3.7
312,976 - -1.3
312,976 - -1.3

Or see this region's nucleotide sequence